[ccp4bb] Faculty positions at Molecular Biophysics Unit, IISc, Bangalore
Dear all Kindly make a note. Indian Institute of Science, Bangalore. Molecular Biophysics Unit (http://mbu.iisc.ernet.in/) Opening for Assistant Professor Positions. Applicants, preferably below 35 years, should have a Ph.D. with postdoctoral experience with an excellent publication record. We seek candidates in the general area of structural biology with an emphasis on understanding macromolecular systems at the molecular level. Applications with a detailed CV, research plan (not exceeding 3 pages) and names of at least 4 referees should be sent to chair...@mbu.iisc.ernet.in Best
[ccp4bb] off topic: ITC program error
Hi All Sorry for this off-topic, but I need an urgent help. We have an Origin 7 version of VP-ITC Microcal. I had shut it down 2 days back and switched it on today. When I am trying to open the VPviewer2000 where I can start my run it says that error as missing last inj. I had tried to run as administrator and it gives me the same message. Tried shutdown and restart, still the same error. Any expert advice about this is awaited. kind regards -- rashmi
Re: [ccp4bb] How to start my XDS journey after HKL2000?
ORGX=1016.0 ORGY=1066.8 !Detector origin (pixels). ORGX=NX/2; ORGY=NY/2 Have you checked the beam centre? It's usually the first thing to check when indexing doesn't give you the expected result. Huw
[ccp4bb] Post-Doctoral Position at Novartis Pharma, Basel, Switzerland
NIBR Presidential Postdoctoral Program Would you like to contribute to innovative research with the goal of improving human health? Novartis Institutes for BioMedical Research has a variety of postdoctoral positions in biology, chemistry, and computational sciences that provide excellent training in research and exposure to science in a pharmaceutical setting. The NIBR Presidential Postdoctoral Program provides scientists with a unique opportunity to conduct innovative, interdisciplinary basic research in an industry setting. Presidential Postdocs have a NIBR mentor and an academic mentor, develop their projects in consultation with both mentors, and are expected to publish their results in leading journals. PhD students in the last year of their doctoral research, as well as postdoctoral fellows with fewer than two years of research experience past their PhD, are eligible to apply. Applications are accepted on a rolling basis. The postdoctoral term is up to 4 years. Postdoctoral position in structural biology, Basel, Switzerland We are currently seeking candidates for a postdoctoral scholar to study the structural biology of cytokine receptor complexes. Qualifications: Candidates should have a thorough understanding of the applicability of biochemical, biophysical and structural techniques in the study of protein complexes and an overarching grasp of structure-function mechanisms relating to protein receptor complexes. Prior experience in at least two of the following areas: protein expression (E. coli, insect cell, mammalian), protein purification, biophysical characterization of protein-protein complexes (e.g. SPR, ITC), and protein structure (crystallization, data collection, structure determination) is essential. Prior experience with the crystallization of glycosylated proteins is also highly desirable. Candidates must be able to work independently as well as participate effectively in team efforts. Excellent verbal and written communication and presentation skills, and scientific creativity are essential. For more information and to apply, please visit http://nibr.com/careers/postdocs.shtml and reference Postdoc position - Cytokine receptor complexes in your cover letter. (Please note: we are unable to accept applications by email). Novartis is an equal opportunity employer committed to embracing and leveraging diverse backgrounds. M/F/D/V. _ CONFIDENTIALITY NOTICE The information contained in this e-mail message is intended only for the exclusive use of the individual or entity named above and may contain information that is privileged, confidential or exempt from disclosure under applicable law. If the reader of this message is not the intended recipient, or the employee or agent responsible for delivery of the message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify the sender immediately by e-mail and delete the material from any computer. Thank you.
[ccp4bb] inflluence of pH for crystallization on protein 3-D structure
Acoot You may be interested in some data-mining that we did a few years ago http://www.douglas.co.uk/PDB_data.htm#Acid-base We didn't see any obvious correlations On 28 October 2012 03:00, Acoot Brett acootbr...@yahoo.com wrote: Dear All, A protein crystal can be got at pH 5 or 8, or a pH with much extreme value. What will be the relatively extreme pH value to get the crystal on the protein structure solved based on the crystal got? I mean usually we regard the physiological pH as 7. If a crystal was got at pH 5, the structure solved may be different from the protein structure at pH 7. But it seems there is rarely analysis on the discrepancy of the protein structures when publishing 3-D structure with the protein crystal got at relatively extreme pH. I am looking forward to getting your comment on it. Cheers, Acoot -- patr...@douglas.co.ukDouglas Instruments Ltd. Douglas House, East Garston, Hungerford, Berkshire, RG17 7HD, UK Directors: Peter Baldock, Patrick Shaw Stewart http://www.douglas.co.uk Tel: 44 (0) 148-864-9090US toll-free 1-877-225-2034 Regd. England 2177994, VAT Reg. GB 480 7371 36 -- patr...@douglas.co.ukDouglas Instruments Ltd. Douglas House, East Garston, Hungerford, Berkshire, RG17 7HD, UK Directors: Peter Baldock, Patrick Shaw Stewart http://www.douglas.co.uk Tel: 44 (0) 148-864-9090US toll-free 1-877-225-2034 Regd. England 2177994, VAT Reg. GB 480 7371 36
Re: [ccp4bb] How to start my XDS journey after HKL2000?
Hi, everyone Now I can get collect result after setting ORGX and ORGY to 1024. Thank Manfred for his help. Thank everyone who helped me. Best regard Chang 2012/10/29 Manfred S. Weiss manfred.we...@helmholtz-berlin.de: The same is true for the third data. I would suggest the following. Examine the images and try to see where the origin is. If you cannot figure this out, set ORGX and ORGY both to 1024, which is the exact center of the detector. And then try again and send me the IDXREF.LP Best wishes Manfred On 29.10.2012 08:35, Chang Qing wrote: 2012/10/29 Chang Qing robie0...@gmail.com: 2012/10/29 Chang Qing robie0...@gmail.com: Thank you very much. I will send you the files through three mail. 2012/10/29 Manfred S. Weiss manfred.we...@helmholtz-berlin.de: Thank you very much. I think this is a trivial error, but I will have to investigate a little bit. Can you also send me the files IDXREF.LP and FRAME.cbf for the three data sets processed with XDS? Of course I will treat your data in confidence. With best wishes Manfred On 29.10.2012 07:54, Chang Qing wrote: 2012/10/29 Chang Qing robie0...@gmail.com: 2012/10/29 Chang Qing robie0...@gmail.com: Dear Manfred Thank you very much. I think I cannot answer your questions in detail. As I am a clinical doctor indeed, I don't know some principles in detail. I will send you all log file. Mail will be separated in three. First letter, data is collected in spring8(41xu), and can get correct result. Second and third letter, date is collected in KEK(nw12a), and space group are wrong. As my data is not publication yet, would you please keep it secret? Thank you very much. As I use macbook, XDSAPP cannot be installed on it. 2012/10/29 Manfred S. Weiss manfred.we...@helmholtz-berlin.de: Dear Chang, there is something seriously wrong with this data. It is possible that the lattice is mis-indexed. Which images did you use for indexing. In contrast to HKL2000, which indexes based on a Fourier analysis of just one images, XDS indexes based on differences vector and needs at least a few (10) images, better would be two slices of 10 images 90 degrees apart. Which XDS tutorial have you followed? Have you tried any of the automatic versions of XDS, such as XDSAPP which we are developing in our lab? Where were the data collected? If you send me some more information about your data I will help you process them using XDS. With best regards Manfred On 29.10.2012 06:03, Chang Qing wrote: And CORRECT file 2012/10/29 Chang Qing robie0...@gmail.com: Here, I show some information about my data and input files of XDS. Wavelength (A) 1. Raster size (mm) 0.10240 (default) Raster size (mm) 0.10240 (default) Film width (mm) 209.72 (default) Film length (mm) 209.72 (default) Record length (pixels) 2048 (default) Number of records 2048 (default) Top limit of useful data 0. (default) Left limit of useful data 0. (default) spots rejected when pixel overflow at value : 65500.0 pixels rejected at value: 0 Oscillation starts at 0. Oscillation range 1. Lattice type: primitive Orientation axis 1 (vertical plane) 1*h 0*k 0*l (default) Orientation axis 2 (spindle) 0*h 0*k 1*l (default) Mosaicity 0.3 CrysZ (beam) axis 0. (default) CrysY (vertical) axis 0. (default) CrysX (spindle) axis 0. (default) unit cell parameters not entered Detector (mis)orientation angles: CassZ (beam) axis 0. CassY (vertical) axis 0. CassX (spindle) axis 0. Detector 2 theta 0. Detector rotation 90.000 (default) Flat detector (default) Detector to crystal distance 166.20 X beam104.80 Y beam105.10 Beam polarization 0.99000 Detector absorption 100.00 (default) Air absorption length 860.00 (default) Crossfire y 0. Crossfire x 0. Crossfire xy 0. Horizontal box size 3.6864 Vertical box size3.6864 2012/10/29 Zhijie Li zhijie...@utoronto.ca: BTW, did you supply XDS with correct beamline/detector information in your input file? Have you compared these information with your HKL2000 site file? If you show us the input file and the HKL2000 site file it might be helpful. -- From: Chang Qing robie0...@gmail.com Sent: Monday, October 29, 2012 12:06 AM To: Zhijie Li zhijie...@utoronto.ca Subject: Re: [ccp4bb] How to start my XDS journey after HKL2000? There were no obvious error messages after XDS. Just, the result of space group was wrong. I can not solve structure through wrong space group. Quality of data is very good. I thought I could get correct result even parameters were in default as recommended
Re: [ccp4bb] Orientation of the crystal and it importance in data collection
-BEGIN PGP SIGNED MESSAGE- Hash: SHA1 Dear B. Vijay, for single-wavelength (as opposed to Laue) X-ray crystallographic data collection it is in general helpful to mount your crystal in an arbitrary orientation. If you happen to mount it such that a symmetry axis is parallel to the rotation axis, you may not be able to collect fully complete data. Indexing routines figure out the orientation of your crystal. After integrating all reflections, the orientation is refined (depending on the integration program you use). For anomalous data you may want to collect in inverse beam mode which makes sure you collect Bijvoet pairs close in time and thus reduce the effect of radiation damage. As drawback you risk possible systematic errors in the Bijvoet pairs, but I am not sure this is a major drawback for MX crystals. I recomend you take a look a Zbigniew Dauter's article Data-collection strategies, Acta Cryst D55 (1999) p. 1703-1717 doi:10.1107/S0907444999008367 Best, Tim On 10/27/2012 07:58 AM, Vijayakumar.B wrote: Dear CCP4BB users, I have some basic questions in the data collection. Please give me some ideas to get clear in this part. 1)Why orientation of the crystal is importance? 2)If we mounted the crystal in arbitrary, what it leads? 3)How to find out crystal misseting angels in the data collection if we mounted arbitrary? 4)What should we make clear before collecting anomalous signal data ? Thanks in advance. With regards B. Vijay - -- Dr Tim Gruene Institut fuer anorganische Chemie Tammannstr. 4 D-37077 Goettingen GPG Key ID = A46BEE1A -BEGIN PGP SIGNATURE- Version: GnuPG v1.4.12 (GNU/Linux) Comment: Using GnuPG with Mozilla - http://enigmail.mozdev.org/ iD8DBQFQjmJQUxlJ7aRr7hoRAmZnAJ9HwuG1ITVs9qKlhUL0kz/yDP+H+ACggEPl VajYAhsCbbDzbPhYmsvArxU= =zkqj -END PGP SIGNATURE-
Re: [ccp4bb] How to start my XDS journey after HKL2000?
I have a question regarding model building. can anyone guide how to find and add water molecules in electron density map in coot. regardsSaleem Date: Mon, 29 Oct 2012 19:23:00 +0900 From: robie0...@gmail.com Subject: Re: [ccp4bb] How to start my XDS journey after HKL2000? To: CCP4BB@JISCMAIL.AC.UK Hi, everyone Now I can get collect result after setting ORGX and ORGY to 1024. Thank Manfred for his help. Thank everyone who helped me. Best regard Chang 2012/10/29 Manfred S. Weiss manfred.we...@helmholtz-berlin.de: The same is true for the third data. I would suggest the following. Examine the images and try to see where the origin is. If you cannot figure this out, set ORGX and ORGY both to 1024, which is the exact center of the detector. And then try again and send me the IDXREF.LP Best wishes Manfred On 29.10.2012 08:35, Chang Qing wrote: 2012/10/29 Chang Qing robie0...@gmail.com: 2012/10/29 Chang Qing robie0...@gmail.com: Thank you very much. I will send you the files through three mail. 2012/10/29 Manfred S. Weiss manfred.we...@helmholtz-berlin.de: Thank you very much. I think this is a trivial error, but I will have to investigate a little bit. Can you also send me the files IDXREF.LP and FRAME.cbf for the three data sets processed with XDS? Of course I will treat your data in confidence. With best wishes Manfred On 29.10.2012 07:54, Chang Qing wrote: 2012/10/29 Chang Qing robie0...@gmail.com: 2012/10/29 Chang Qing robie0...@gmail.com: Dear Manfred Thank you very much. I think I cannot answer your questions in detail. As I am a clinical doctor indeed, I don't know some principles in detail. I will send you all log file. Mail will be separated in three. First letter, data is collected in spring8(41xu), and can get correct result. Second and third letter, date is collected in KEK(nw12a), and space group are wrong. As my data is not publication yet, would you please keep it secret? Thank you very much. As I use macbook, XDSAPP cannot be installed on it. 2012/10/29 Manfred S. Weiss manfred.we...@helmholtz-berlin.de: Dear Chang, there is something seriously wrong with this data. It is possible that the lattice is mis-indexed. Which images did you use for indexing. In contrast to HKL2000, which indexes based on a Fourier analysis of just one images, XDS indexes based on differences vector and needs at least a few (10) images, better would be two slices of 10 images 90 degrees apart. Which XDS tutorial have you followed? Have you tried any of the automatic versions of XDS, such as XDSAPP which we are developing in our lab? Where were the data collected? If you send me some more information about your data I will help you process them using XDS. With best regards Manfred On 29.10.2012 06:03, Chang Qing wrote: And CORRECT file 2012/10/29 Chang Qing robie0...@gmail.com: Here, I show some information about my data and input files of XDS. Wavelength (A) 1. Raster size (mm) 0.10240 (default) Raster size (mm) 0.10240 (default) Film width (mm) 209.72 (default) Film length (mm) 209.72 (default) Record length (pixels) 2048 (default) Number of records 2048 (default) Top limit of useful data 0. (default) Left limit of useful data 0. (default) spots rejected when pixel overflow at value : 65500.0 pixels rejected at value: 0 Oscillation starts at 0. Oscillation range 1. Lattice type: primitive Orientation axis 1 (vertical plane) 1*h 0*k 0*l (default) Orientation axis 2 (spindle) 0*h 0*k 1*l (default) Mosaicity 0.3 CrysZ (beam) axis 0. (default) CrysY (vertical) axis 0. (default) CrysX (spindle) axis 0. (default) unit cell parameters not entered Detector (mis)orientation angles: CassZ (beam) axis 0. CassY (vertical) axis 0. CassX (spindle) axis 0. Detector 2 theta 0. Detector rotation 90.000 (default) Flat detector (default) Detector to crystal distance 166.20 X beam104.80 Y beam105.10 Beam polarization 0.99000 Detector absorption 100.00 (default) Air absorption length 860.00 (default) Crossfire y 0. Crossfire x 0. Crossfire xy 0. Horizontal box size 3.6864 Vertical box size3.6864 2012/10/29 Zhijie Li zhijie...@utoronto.ca: BTW, did you supply XDS with correct beamline/detector information in your input file? Have you compared these information with your HKL2000 site file? If you show us the input file and the HKL2000 site file it might be helpful.
[ccp4bb] how to find and add water molecules in electron density map in coot??
I have a question regarding model building. can anyone guide how to find and add water molecules in electron density map in coot. regardsSaleem Date: Mon, 29 Oct 2012 19:23:00 +0900 From: robie0...@gmail.com Subject: Re: [ccp4bb] How to start my XDS journey after HKL2000? To: CCP4BB@JISCMAIL.AC.UK Hi, everyone Now I can get collect result after setting ORGX and ORGY to 1024. Thank Manfred for his help. Thank everyone who helped me. Best regard Chang 2012/10/29 Manfred S. Weiss manfred.we...@helmholtz-berlin.de: The same is true for the third data. I would suggest the following. Examine the images and try to see where the origin is. If you cannot figure this out, set ORGX and ORGY both to 1024, which is the exact center of the detector. And then try again and send me the IDXREF.LP Best wishes Manfred On 29.10.2012 08:35, Chang Qing wrote: 2012/10/29 Chang Qing robie0...@gmail.com: 2012/10/29 Chang Qing robie0...@gmail.com: Thank you very much. I will send you the files through three mail. 2012/10/29 Manfred S. Weiss manfred.we...@helmholtz-berlin.de: Thank you very much. I think this is a trivial error, but I will have to investigate a little bit. Can you also send me the files IDXREF.LP and FRAME.cbf for the three data sets processed with XDS? Of course I will treat your data in confidence. With best wishes Manfred On 29.10.2012 07:54, Chang Qing wrote: 2012/10/29 Chang Qing robie0...@gmail.com: 2012/10/29 Chang Qing robie0...@gmail.com: Dear Manfred Thank you very much. I think I cannot answer your questions in detail. As I am a clinical doctor indeed, I don't know some principles in detail. I will send you all log file. Mail will be separated in three. First letter, data is collected in spring8(41xu), and can get correct result. Second and third letter, date is collected in KEK(nw12a), and space group are wrong. As my data is not publication yet, would you please keep it secret? Thank you very much. As I use macbook, XDSAPP cannot be installed on it. 2012/10/29 Manfred S. Weiss manfred.we...@helmholtz-berlin.de: Dear Chang, there is something seriously wrong with this data. It is possible that the lattice is mis-indexed. Which images did you use for indexing. In contrast to HKL2000, which indexes based on a Fourier analysis of just one images, XDS indexes based on differences vector and needs at least a few (10) images, better would be two slices of 10 images 90 degrees apart. Which XDS tutorial have you followed? Have you tried any of the automatic versions of XDS, such as XDSAPP which we are developing in our lab? Where were the data collected? If you send me some more information about your data I will help you process them using XDS. With best regards Manfred On 29.10.2012 06:03, Chang Qing wrote: And CORRECT file 2012/10/29 Chang Qing robie0...@gmail.com: Here, I show some information about my data and input files of XDS. Wavelength (A) 1. Raster size (mm) 0.10240 (default) Raster size (mm) 0.10240 (default) Film width (mm) 209.72 (default) Film length (mm) 209.72 (default) Record length (pixels) 2048 (default) Number of records 2048 (default) Top limit of useful data 0. (default) Left limit of useful data 0. (default) spots rejected when pixel overflow at value : 65500.0 pixels rejected at value: 0 Oscillation starts at 0. Oscillation range 1. Lattice type: primitive Orientation axis 1 (vertical plane) 1*h 0*k 0*l (default) Orientation axis 2 (spindle) 0*h 0*k 1*l (default) Mosaicity 0.3 CrysZ (beam) axis 0. (default) CrysY (vertical) axis 0. (default) CrysX (spindle) axis 0. (default) unit cell parameters not entered Detector (mis)orientation angles: CassZ (beam) axis 0. CassY (vertical) axis 0. CassX (spindle) axis 0. Detector 2 theta 0. Detector rotation 90.000 (default) Flat detector (default) Detector to crystal distance 166.20 X beam104.80 Y beam105.10 Beam polarization 0.99000 Detector absorption 100.00 (default) Air absorption length 860.00 (default) Crossfire y 0. Crossfire x 0. Crossfire xy 0. Horizontal box size 3.6864 Vertical box size3.6864 2012/10/29 Zhijie Li zhijie...@utoronto.ca: BTW, did you supply XDS with correct beamline/detector information in your input file? Have you compared these information with your HKL2000 site file? If you show us the input file and the HKL2000 site file it might be helpful.
Re: [ccp4bb] Orientation of the crystal and it importance in data collection
Dear Tim and B. Vijay, On Mon, 2012-10-29 at 12:02 +0100, Tim Gruene wrote: Dear B. Vijay, for single-wavelength (as opposed to Laue) X-ray crystallographic data collection it is in general helpful to mount your crystal in an arbitrary orientation. Well, this depends on the sample, and how you are going to solve the structure if you don't already know it for the crystal form that you have (i.e. MR, SAD, MAD etc). The overall oscillation range required for completeness depends on the orientation, and if your sample is radiation sensitive then achieving (near) completeness early can be helpful. If you happen to mount it such that a symmetry axis is parallel to the rotation axis, you may not be able to collect fully complete data. and also if the symmetry axis is a screw axis you won't have observed any systematic absences. So it may be a good idea to tilt the symmetry axis away from the rotation axis a bit. But (in some cases) not too far: apart from the rotation range issue, if you have one cell axis much longer than the others, putting it close to the rotation axis will reduce spot overlap, which can also be helpful. These factors (as well as others such as anisotropy of the sample) fight against each other, and the best compromise depends on the sample, the wavelength and the instrumentation that you are using. A truly arbitrary orientation risks getting it badly wrong. If you are unlucky you may then be unable to process the images and/or solve the structure (or at least have severe problems). Indexing routines figure out the orientation of your crystal. After integrating all reflections, the orientation is refined (depending on the integration program you use). For anomalous data you may want to collect in inverse beam mode which makes sure you collect Bijvoet pairs close in time and thus reduce the effect of radiation damage. As drawback you risk possible systematic errors in the Bijvoet pairs, but I am not sure this is a major drawback for MX crystals. If you can adjust the orientation so that Bijvoet pairs are on the same image, this can help here. I recomend you take a look a Zbigniew Dauter's article Data-collection strategies, Acta Cryst D55 (1999) p. 1703-1717 doi:10.1107/S0907444999008367 This is of course excellent advice. Regards, Peter. Best, Tim On 10/27/2012 07:58 AM, Vijayakumar.B wrote: Dear CCP4BB users, I have some basic questions in the data collection. Please give me some ideas to get clear in this part. 1)Why orientation of the crystal is importance? 2)If we mounted the crystal in arbitrary, what it leads? 3)How to find out crystal misseting angels in the data collection if we mounted arbitrary? 4)What should we make clear before collecting anomalous signal data ? Thanks in advance. With regards B. Vijay -- Peter Keller Tel.: +44 (0)1223 353033 Global Phasing Ltd., Fax.: +44 (0)1223 366889 Sheraton House, Castle Park, Cambridge CB3 0AX United Kingdom
[ccp4bb] Different residue type with same number in refmac?
Hello, The protein preparation I'm working with is not homogeneous. It comes from a natural source and few amino acid are not conserved. Is there a way to take this into account in refmac5. In other words having two alternative conformations with two different residue names? Thanks for your help. Fred - Frédéric Kerff Chercheur qualifié F.R.S.-FNRS Cristallographie des protéines Centre d'Ingénierie des Protéines Université de Liège 17, Allée du 6 Août - Bat B5a 4000 Liège (Belgium) Tel.: +32 (0)4 3663620 Fax: +32 (0)4 3663772
Re: [ccp4bb] Different residue type with same number in refmac?
Yes. It's not different from two alternate conformations of the same residue as far as refmac is concerned. Just name one AXXX, the other BYYY and give them each occupancies less than 1. Refmac5 will handle them properly. Boaz Boaz Shaanan, Ph.D. Dept. of Life Sciences Ben-Gurion University of the Negev Beer-Sheva 84105 Israel E-mail: bshaa...@bgu.ac.il Phone: 972-8-647-2220 Skype: boaz.shaanan Fax: 972-8-647-2992 or 972-8-646-1710 From: CCP4 bulletin board [CCP4BB@JISCMAIL.AC.UK] on behalf of Fred Kerff [fke...@ulg.ac.be] Sent: Monday, October 29, 2012 3:58 PM To: CCP4BB@JISCMAIL.AC.UK Subject: [ccp4bb] Different residue type with same number in refmac? Hello, The protein preparation I'm working with is not homogeneous. It comes from a natural source and few amino acid are not conserved. Is there a way to take this into account in refmac5. In other words having two alternative conformations with two different residue names? Thanks for your help. Fred - Frédéric Kerff Chercheur qualifié F.R.S.-FNRS Cristallographie des protéines Centre d'Ingénierie des Protéines Université de Liège 17, Allée du 6 Août - Bat B5a 4000 Liège (Belgium) Tel.: +32 (0)4 3663620 Fax: +32 (0)4 3663772
Re: [ccp4bb] inflluence of pH for crystallization on protein 3-D structure
An example for how crystallization at non-physiological pH (and non-physiological concentrations and non-physiological environmental) may influence behavior of the protein is the Botulinum A LC protease. Under normal conditions (upon endocytosis of a few molecules at best into the nerve cell), it works like a canonical serine protease, but at pH 4.6 it cleaves itself in a non-canonical fashion, i.e. with a loop resembling the target peptide running opposite direction. Later isoform structures solved at neutral pH showed normal, canonical protease activity. http://www.ruppweb.org/cvs/br/Segelke_2004_PNAS_botulinum_neurotoxin.pdf BR From: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] On Behalf Of Acoot Brett Sent: Saturday, October 27, 2012 8:01 PM To: CCP4BB@JISCMAIL.AC.UK Subject: [ccp4bb] inflluence of pH for crystallization on protein 3-D structure Dear All, A protein crystal can be got at pH 5 or 8, or a pH with much extreme value. What will be the relatively extreme pH value to get the crystal on the protein structure solved based on the crystal got? I mean usually we regard the physiological pH as 7. If a crystal was got at pH 5, the structure solved may be different from the protein structure at pH 7. But it seems there is rarely analysis on the discrepancy of the protein structures when publishing 3-D structure with the protein crystal got at relatively extreme pH. I am looking forward to getting your comment on it. Cheers, Acoot
[ccp4bb] Postdoc vacancy in the Boettcher lab - Edinburgh
Dear Colleagues, I am forwarding this message from Bettina Boettcher. Please get in touch with her if you have any queries about the opening. Best regards, Laura I have a PostDoc position in my lab at the University of Edinburgh. I am looking for a good biochemist with a keen interest to work on the structure determination of the Ccr4Not complex. A detailed job description can be found at: https://www.vacancies.ed.ac.uk the vacancy ref is: 005386 (please include leading zeros) The deadline for applications is 26.11.2012 Interviews will be during the first week of December. The anticipated start date for the position is not later than 16.1.2013. Best regards, Bettina Boettcher The University of Edinburgh is a charitable body, registered in Scotland, with registration number SC005336.
Re: [ccp4bb] Faculty positions at Molecular Biophysics Unit, IISc, Bangalore
Dear Friends, There is no need to apply to this position, we suggest. It is a PREDETERMINED SELECTION, i.e. candidate is fixed and this (advertisement, screening, selection board, selection and approval) is just the procedure. It does not matter whether you apply or not. If you apply and called for interview, then you have to waste your valuable time as well as huge travel money unless some Big Boss is fixing you to the post. Interestingly Indian Institute of Science recruits and carries faculties and trains them in such a way that it has become a epicentre of recruitment scams across India and it make rest of Indian Scientists/Faculties in their path of scams and CRIME. Students also inherit the character of their boss. They do not participate in any form of fair selection in the country. Almost all cases they select and load many times inferior candidates even though candidate was not seen by anybody or interviewed. Similarly they distribute various national awards among themselves and within their group. THEY ARE NOT ASHAMED AT ALL. This is just an attempt of WASTING HUGE PUBLIC MONEY by a bunch of crooks who are good for nothing but worst for everything. Sham Date: Mon, 29 Oct 2012 11:47:06 +0530 From: vikasnavra...@gmail.com Subject: [ccp4bb] Faculty positions at Molecular Biophysics Unit, IISc, Bangalore To: CCP4BB@JISCMAIL.AC.UK Dear all Kindly make a note. Indian Institute of Science, Bangalore. Molecular Biophysics Unit (http://mbu.iisc.ernet.in/) Opening for Assistant Professor Positions. Applicants, preferably below 35 years, should have a Ph.D. with postdoctoral experience with an excellent publication record. We seek candidates in the general area of structural biology with an emphasis on understanding macromolecular systems at the molecular level. Applications with a detailed CV, research plan (not exceeding 3 pages) and names of at least 4 referees should be sent to chair...@mbu.iisc.ernet.in Best
[ccp4bb] XFEL and Synchrotron Computation at LBNL: Postdoc and Proj Scientist Positions
Dear Colleagues: Two NIH-funded positions are available immediately in the Computational Crystallography Initiative group (http://cci.lbl.gov) at Lawrence Berkeley National Laboratory. Massive datasets generated by ultrafast detectors at both conventional sources and X-ray free-electron lasers are posing profound computational challenges. In response I am seeking a Postdoctoral Fellow and a Project Scientist to develop the next generation of data reduction software. Physics-based algorithms will be derived to model the data, and multiprocessing hardware (including GPUs) will be deployed for computation. For synchrotron sources, goals include the ability to integrate data from multiple overlapping lattices, as well as marginal data from poorly diffracting samples. For XFELs, we seek to understand how to correctly merge data from serial femtosecond still shots, containing only partially recorded Bragg reflections, with sufficient accuracy to enable de novo phasing. The end product will be new open-source code to be deployed at light sources worldwide in collaboration with numerous international partners. There will be close collaboration with synchrotron beamline groups at ALS, SSRL, APS and Diamond Light Source, as well as with leading crystallographers performing XFEL experiments at LCLS. Individuals with extensive experience in computational and/or experimental crystallography are ideal, especially those who have thought about the mathematics of diffraction. Applicants are also welcome from other bioimaging backgrounds. I particularly encourage those who have a track record of bringing new methods and tools to fruition. Informal inquires may be sent directly to me. Formal job listings are posted at: Postdoctoral Fellow: https://lbl.taleo.net/careersection/2/jobdetail.ftl?lang=enjob=75266 Project Scientist: https://lbl.taleo.net/careersection/2/jobdetail.ftl?lang=enjob=75269 Best regards, Nick Sauter -- Nicholas K. Sauter, Ph. D. Computer Staff Scientist, Physical Biosciences Division Lawrence Berkeley National Laboratory 1 Cyclotron Rd., Bldg. 64R0121 Berkeley, CA 94720-8118 (510) 486-5713 Web: http://cci.lbl.gov/jobs
Re: [ccp4bb] Faculty positions at Molecular Biophysics Unit, IISc, Bangalore
Sham, who so ever you are, if you have such a long list of complaints, why don't you put your name clearly and complain openly, instead of hiding behind some anonymous 'SHAM' name. What you write about IISc may be all true or it may be reflection of your frustration for not getting a job at IISc! How do we know? So, grow a 'spine', if you have a complaint, say it like a man and do not hide behind and bad mouth others anonymously like spoiled child. You are not only throwing dirt on such a prestigious Indian institution, and also on many decent and capable scientists who do outstanding work and produce brilliant graduate students, some of which I was fortunate to have in my lab. Best Narayana Sthanam -- Narayana Sthanam,Ph D Professor of Structural Biology 244 CBSE 1025 18th Street South Center for Biophysical Sciences and Engineering University of Alabama at Birmingham Birmingham, Al 35294 Phone: 205 934 0119 URL: http://www.opt.uab.edu/narayanalabhttps://mail.ad.uab.edu/owa/redir.aspx?C=80a7a9ef03ea46b58d648b78f13d4272URL=http%3a%2f%2fwww.opt.uab.edu%2fnarayanalab Never let success go to your head, nor failure to your heart From: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] On Behalf Of U US Sent: Monday, October 29, 2012 12:03 PM To: CCP4BB@JISCMAIL.AC.UK Subject: Re: [ccp4bb] Faculty positions at Molecular Biophysics Unit, IISc, Bangalore Dear Friends, There is no need to apply to this position, we suggest. It is a PREDETERMINED SELECTION, i.e. candidate is fixed and this (advertisement, screening, selection board, selection and approval) is just the procedure. It does not matter whether you apply or not. If you apply and called for interview, then you have to waste your valuable time as well as huge travel money unless some Big Boss is fixing you to the post. Interestingly Indian Institute of Science recruits and carries faculties and trains them in such a way that it has become a epicentre of recruitment scams across India and it make rest of Indian Scientists/Faculties in their path of scams and CRIME. Students also inherit the character of their boss. They do not participate in any form of fair selection in the country. Almost all cases they select and load many times inferior candidates even though candidate was not seen by anybody or interviewed. Similarly they distribute various national awards among themselves and within their group. THEY ARE NOT ASHAMED AT ALL. This is just an attempt of WASTING HUGE PUBLIC MONEY by a bunch of crooks who are good for nothing but worst for everything. Sham Date: Mon, 29 Oct 2012 11:47:06 +0530 From: vikasnavra...@gmail.commailto:vikasnavra...@gmail.com Subject: [ccp4bb] Faculty positions at Molecular Biophysics Unit, IISc, Bangalore To: CCP4BB@JISCMAIL.AC.UKmailto:CCP4BB@JISCMAIL.AC.UK Dear all Kindly make a note. Indian Institute of Science, Bangalore. Molecular Biophysics Unit (http://mbu.iisc.ernet.in/) Opening for Assistant Professor Positions. Applicants, preferably below 35 years, should have a Ph.D. with postdoctoral experience with an excellent publication record. We seek candidates in the general area of structural biology with an emphasis on understanding macromolecular systems at the molecular level. Applications with a detailed CV, research plan (not exceeding 3 pages) and names of at least 4 referees should be sent to chair...@mbu.iisc.ernet.inmailto:chair...@mbu.iisc.ernet.in Best
Re: [ccp4bb] Faculty positions at Molecular Biophysics Unit, IISc, Bangalore
My dear Sham, Who ever you are, first I would like to tell you, if you are not aware, that CCP4bb is not a platform for the kind of you mail you have written. I am an alumnus of MBU (IISc) and would be happy to answer your doubts emerged of frustrations. I am sending this mail to the whole group, however, I invite you to discuss about this personally with me if you dare. Perhaps CCP4bb moderators should warn/block such anonymous individuals for writing this kind of rubbish mails. Thanks Alok On Mon, Oct 29, 2012 at 1:03 PM, U US u...@hotmail.com wrote: Dear Friends, There is no need to apply to this position, we suggest. It is a PREDETERMINED SELECTION, i.e. candidate is fixed and this (advertisement, screening, selection board, selection and approval) is just the procedure. It does not matter whether you apply or not. If you apply and called for interview, then you have to waste your valuable time as well as huge travel money unless some Big Boss is fixing you to the post. Interestingly Indian Institute of Science recruits and carries faculties and trains them in such a way that it has become a epicentre of recruitment scams across India and it make rest of Indian Scientists/Faculties in their path of scams and CRIME. Students also inherit the character of their boss. They do not participate in any form of fair selection in the country. Almost all cases they select and load many times inferior candidates even though candidate was not seen by anybody or interviewed. Similarly they distribute various national awards among themselves and within their group. THEY ARE NOT ASHAMED AT ALL. This is just an attempt of WASTING HUGE PUBLIC MONEY by a bunch of crooks who are good for nothing but worst for everything. Sham -- Date: Mon, 29 Oct 2012 11:47:06 +0530 From: vikasnavra...@gmail.com Subject: [ccp4bb] Faculty positions at Molecular Biophysics Unit, IISc, Bangalore To: CCP4BB@JISCMAIL.AC.UK Dear all Kindly make a note. Indian Institute of Science, Bangalore. Molecular Biophysics Unit (http://mbu.iisc.ernet.in/) Opening for Assistant Professor Positions. Applicants, preferably below 35 years, should have a Ph.D. with postdoctoral experience with an excellent publication record. We seek candidates in the general area of structural biology with an emphasis on understanding macromolecular systems at the molecular level. Applications with a detailed CV, research plan (not exceeding 3 pages) and names of at least 4 referees should be sent to chair...@mbu.iisc.ernet.in Best
Re: [ccp4bb] Faculty positions at Molecular Biophysics Unit, IISc, Bangalore
This is not a reply to the post by some one called Sham or the original post calling for a faculty position. Everyone here who ever had an experience to be an IIScian and especially any one who been fortunate to experience Molecualr Biophysics Unit will disagree with what was writen about the faculty and the quality of science of MBU. I strongly disagree with students who are a part of MBU at one time turned unfortunately to be thugs to write such deleterious mails. My experiecne about MBU is definitely not same as what is written here. All this mail intend to do is (and i am sure all MBUians will agree) to express strong support for MBU and its great tradition. Padayatti PS Polgenix Inc., Cleveland, Ohio On Mon, Oct 29, 2012 at 2:17 AM, vikas navratna vikasnavra...@gmail.comwrote: Dear all Kindly make a note. Indian Institute of Science, Bangalore. Molecular Biophysics Unit (http://mbu.iisc.ernet.in/) Opening for Assistant Professor Positions. Applicants, preferably below 35 years, should have a Ph.D. with postdoctoral experience with an excellent publication record. We seek candidates in the general area of structural biology with an emphasis on understanding macromolecular systems at the molecular level. Applications with a detailed CV, research plan (not exceeding 3 pages) and names of at least 4 referees should be sent to chair...@mbu.iisc.ernet.in Best -- PSP
[ccp4bb] Very sorry for one more message == RE: [ccp4bb] Faculty positions at Molecular Biophysics Unit, IISc, Bangalore
I fully agree with Pius. I am very sorry other CCP users for sending one more message and filling your mail box for some crazy guys reply. Thanks, Mathews -Original Message- From: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] On Behalf Of Pius Padayatti Sent: Monday, October 29, 2012 10:58 AM To: CCP4BB@JISCMAIL.AC.UK Subject: Re: [ccp4bb] Faculty positions at Molecular Biophysics Unit, IISc, Bangalore This is not a reply to the post by some one called Sham or the original post calling for a faculty position. Everyone here who ever had an experience to be an IIScian and especially any one who been fortunate to experience Molecualr Biophysics Unit will disagree with what was writen about the faculty and the quality of science of MBU. I strongly disagree with students who are a part of MBU at one time turned unfortunately to be thugs to write such deleterious mails. My experiecne about MBU is definitely not same as what is written here. All this mail intend to do is (and i am sure all MBUians will agree) to express strong support for MBU and its great tradition. Padayatti PS Polgenix Inc., Cleveland, Ohio On Mon, Oct 29, 2012 at 2:17 AM, vikas navratna vikasnavra...@gmail.com wrote: Dear all Kindly make a note. Indian Institute of Science, Bangalore. Molecular Biophysics Unit (http://mbu.iisc.ernet.in/) Opening for Assistant Professor Positions. Applicants, preferably below 35 years, should have a Ph.D. with postdoctoral experience with an excellent publication record. We seek candidates in the general area of structural biology with an emphasis on understanding macromolecular systems at the molecular level. Applications with a detailed CV, research plan (not exceeding 3 pages) and names of at least 4 referees should be sent to chair...@mbu.iisc.ernet.in Best -- PSP
Re: [ccp4bb] Very sorry for one more message == RE: [ccp4bb] Faculty positions at Molecular Biophysics Unit, IISc, Bangalore
As an IISc alumnus and Ex-MBUite, I outright reject the contents of the email posted by Sham. It is sad to see how a platform such as CCP4BB, which is so educational and informative is being misused by one individual to spread false message and discredit one of the esteemed institute of higher learning. Regrettably, Sridhar Prasad On 10/29/2012 11:09 AM, Mathews, Irimpan I. wrote: I fully agree with Pius. I am very sorry other CCP users for sending one more message and filling your mail box for some crazy guys reply. Thanks, Mathews -Original Message- From: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] On Behalf Of Pius Padayatti Sent: Monday, October 29, 2012 10:58 AM To: CCP4BB@JISCMAIL.AC.UK Subject: Re: [ccp4bb] Faculty positions at Molecular Biophysics Unit, IISc, Bangalore This is not a reply to the post by some one called Sham or the original post calling for a faculty position. Everyone here who ever had an experience to be an IIScian and especially any one who been fortunate to experience Molecualr Biophysics Unit will disagree with what was writen about the faculty and the quality of science of MBU. I strongly disagree with students who are a part of MBU at one time turned unfortunately to be thugs to write such deleterious mails. My experiecne about MBU is definitely not same as what is written here. All this mail intend to do is (and i am sure all MBUians will agree) to express strong support for MBU and its great tradition. Padayatti PS Polgenix Inc., Cleveland, Ohio On Mon, Oct 29, 2012 at 2:17 AM, vikas navratna vikasnavra...@gmail.com wrote: Dear all Kindly make a note. Indian Institute of Science, Bangalore. Molecular Biophysics Unit (http://mbu.iisc.ernet.in/) Opening for Assistant Professor Positions. Applicants, preferably below 35 years, should have a Ph.D. with postdoctoral experience with an excellent publication record. We seek candidates in the general area of structural biology with an emphasis on understanding macromolecular systems at the molecular level. Applications with a detailed CV, research plan (not exceeding 3 pages) and names of at least 4 referees should be sent to chair...@mbu.iisc.ernet.in Best attachment: sprasad.vcf
Re: [ccp4bb] Faculty positions at Molecular Biophysics Unit, IISc, Bangalore
Sham is very right about the institute. Narayana Sthanam's illustrious former colleague Krishna Murthy who faked so many structures retracted quite a few papers was an alumnus of the institute. On Mon, Oct 29, 2012 at 12:21 PM, James Stroud xtald...@gmail.com wrote: The agism in the advertisement doesn't do the institute much credit. I'm inclined to believe Sham, given the Institutes stated policies: Applicants, preferably below 35 years James On Oct 29, 2012, at 11:28 AM, Narayana VL Sthanam wrote: Sham, who so ever you are, if you have such a long list of complaints, why don’t you put your name clearly and complain openly, instead of hiding behind some anonymous ‘SHAM’ name. What you write about IISc may be all true or it may be reflection of your frustration for not getting a job at IISc!*** * How do we know? So, grow a ‘spine’, if you have a complaint, say it like a man and do not hide behind and bad mouth others anonymously like spoiled child. You are not only throwing dirt on such a prestigious Indian institution, and also on many decent and capable scientists who do outstanding work and produce brilliant graduate students, some of which I was fortunate to have in my lab. ** ** Best Narayana Sthanam ** ** *--* *Narayana Sthanam,Ph D* *Professor of Structural Biology* *244 CBSE 1025 18th Street South* *Center for Biophysical Sciences and Engineering* *University of Alabama at Birmingham* *Birmingham, Al 35294* *Phone: 205 934 0119* *URL: **http://www.opt.uab.edu/narayanalab*https://mail.ad.uab.edu/owa/redir.aspx?C=80a7a9ef03ea46b58d648b78f13d4272URL=http%3a%2f%2fwww.opt.uab.edu%2fnarayanalab “Never let success go to your head, nor failure to your heart ” ** ** ** ** *From:* CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] *On Behalf Of *U US *Sent:* Monday, October 29, 2012 12:03 PM *To:* CCP4BB@JISCMAIL.AC.UK *Subject:* Re: [ccp4bb] Faculty positions at Molecular Biophysics Unit, IISc, Bangalore ** ** Dear Friends, There is no need to apply to this position, we suggest. It is a PREDETERMINED SELECTION, i.e. candidate is fixed and this (advertisement, screening, selection board, selection and approval) is just the procedure. It does not matter whether you apply or not. If you apply and called for interview, then you have to waste your valuable time as well as huge travel money unless some Big Boss is fixing you to the post. Interestingly Indian Institute of Science recruits and carries faculties and trains them in such a way that it has become a epicentre of recruitment scams across India and it make rest of Indian Scientists/Faculties in their path of scams and CRIME. Students also inherit the character of their boss. They do not participate in any form of fair selection in the country. Almost all cases they select and load many times inferior candidates even though candidate was not seen by anybody or interviewed. Similarly they distribute various national awards among themselves and within their group. THEY ARE NOT ASHAMED AT ALL. This is just an attempt of WASTING HUGE PUBLIC MONEY by a bunch of crooks who are good for nothing but worst for everything. Sham ** ** -- Date: Mon, 29 Oct 2012 11:47:06 +0530 From: vikasnavra...@gmail.com Subject: [ccp4bb] Faculty positions at Molecular Biophysics Unit, IISc, Bangalore To: CCP4BB@JISCMAIL.AC.UK Dear all Kindly make a note. Indian Institute of Science, Bangalore. ** ** Molecular Biophysics Unit (http://mbu.iisc.ernet.in/) ** ** Opening for Assistant Professor Positions. ** ** Applicants, preferably below 35 years, should have a Ph.D. with postdoctoral experience with an excellent publication record. ** ** We seek candidates in the general area of structural biology with an emphasis on understanding macromolecular systems at the molecular level. ** ** Applications with a detailed CV, research plan (not exceeding 3 pages) and names of at least 4 referees should be sent to chair...@mbu.iisc.ernet.in Best
Re: [ccp4bb] Faculty positions at Molecular Biophysics Unit, IISc, Bangalore
Please allow me to share with you some history here. Late Prof. GN Ramachandran (of Ramachandran Plot) was a faculty of MBU, IISC. If you want to find out yourself how good/bad it is, please register for the upcoming conference on International Conference on Biomolecular Forms and Functions: celebrating 50 years of the Ramachandran Map. Chitta P.S. The agism means: 'we prefer assistant professors who are not too old' (35 years according to Indian life expectancy is 45 years in the US). Chitta Das Assistant Professor of Chemistry Department of Chemistry Purdue University 560 Oval Drive West Lafayette IN 47907 Ph. 765 494 5478 http://www.chem.purdue.edu/das/ - Original Message - From: James Stroud xtald...@gmail.com To: CCP4BB@JISCMAIL.AC.UK Sent: Monday, October 29, 2012 3:21:51 PM Subject: Re: [ccp4bb] Faculty positions at Molecular Biophysics Unit, IISc, Bangalore The agism in the advertisement doesn't do the institute much credit. I'm inclined to believe Sham, given the Institutes stated policies: Applicants, preferably below 35 years James On Oct 29, 2012, at 11:28 AM, Narayana VL Sthanam wrote: Sham, who so ever you are, if you have such a long list of complaints, why don’t you put your name clearly and complain openly, instead of hiding behind some anonymous ‘SHAM’ name. What you write about IISc may be all true or it may be reflection of your frustration for not getting a job at IISc! How do we know? So, grow a ‘spine’, if you have a complaint, say it like a man and do not hide behind and bad mouth others anonymously like spoiled child. You are not only throwing dirt on such a prestigious Indian institution, and also on many decent and capable scientists who do outstanding work and produce brilliant graduate students, some of which I was fortunate to have in my lab. Best Narayana Sthanam -- Narayana Sthanam,Ph D Professor of Structural Biology 244 CBSE 1025 18th Street South Center for Biophysical Sciences and Engineering University of Alabama at Birmingham Birmingham, Al 35294 Phone: 205 934 0119 URL: http://www.opt.uab.edu/narayanalab “ Never let success go to your head, nor failure to your heart ” From: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] On Behalf Of U US Sent: Monday, October 29, 2012 12:03 PM To: CCP4BB@JISCMAIL.AC.UK Subject: Re: [ccp4bb] Faculty positions at Molecular Biophysics Unit, IISc, Bangalore Dear Friends, There is no need to apply to this position, we suggest. It is a PREDETERMINED SELECTION, i.e. candidate is fixed and this (advertisement, screening, selection board, selection and approval) is just the procedure. It does not matter whether you apply or not. If you apply and called for interview, then you have to waste your valuable time as well as huge travel money unless some Big Boss is fixing you to the post. Interestingly Indian Institute of Science recruits and carries faculties and trains them in such a way that it has become a epicentre of recruitment scams across India and it make rest of Indian Scientists/Faculties in their path of scams and CRIME. Students also inherit the character of their boss. They do not participate in any form of fair selection in the country. Almost all cases they select and load many times inferior candidates even though candidate was not seen by anybody or interviewed. Similarly they distribute various national awards among themselves and within their group. THEY ARE NOT ASHAMED AT ALL. This is just an attempt of WASTING HUGE PUBLIC MONEY by a bunch of crooks who are good for nothing but worst for everything. Sham Date: Mon, 29 Oct 2012 11:47:06 +0530 From: vikasnavra...@gmail.com Subject: [ccp4bb] Faculty positions at Molecular Biophysics Unit, IISc, Bangalore To: CCP4BB@JISCMAIL.AC.UK Dear all Kindly make a note. Indian Institute of Science, Bangalore. Molecular Biophysics Unit ( http://mbu.iisc.ernet.in/ ) Opening for Assistant Professor Positions. Applicants, preferably below 35 years, should have a Ph.D. with postdoctoral experience with an excellent publication record. We seek candidates in the general area of structural biology with an emphasis on understanding macromolecular systems at the molecular level. Applications with a detailed CV, research plan (not exceeding 3 pages) and names of at least 4 referees should be sent to chair...@mbu.iisc.ernet.in Best
Re: [ccp4bb] Faculty positions at Molecular Biophysics Unit, IISc, Bangalore
Just to let Narayan Viswam know more about HM Krishnamurthy. He did his PhD from IISc long long back in 1979 on small molecule crystallography. Then he did his postdoc in protein crystallography from two top most Universities of USA. He had been mentored by really really great protein crystallographers in USA. Everybody will agree that neither the Institute nor the mentors should be blamed for such acts. This is individual's character and act. I am sure that there are many more frustrated people who did not get through the prestigious institute at some level of their life. Such comments deserve nothing more than the ignorance. Seems grapes are sour :) MT On Tue, Oct 30, 2012 at 1:08 AM, Narayan Viswam nvisw...@gmail.com wrote: Sham is very right about the institute. Narayana Sthanam's illustrious former colleague Krishna Murthy who faked so many structures retracted quite a few papers was an alumnus of the institute. On Mon, Oct 29, 2012 at 12:21 PM, James Stroud xtald...@gmail.com wrote: The agism in the advertisement doesn't do the institute much credit. I'm inclined to believe Sham, given the Institutes stated policies: Applicants, preferably below 35 years James On Oct 29, 2012, at 11:28 AM, Narayana VL Sthanam wrote: Sham, who so ever you are, if you have such a long list of complaints, why don’t you put your name clearly and complain openly, instead of hiding behind some anonymous ‘SHAM’ name. What you write about IISc may be all true or it may be reflection of your frustration for not getting a job at IISc!** ** How do we know? So, grow a ‘spine’, if you have a complaint, say it like a man and do not hide behind and bad mouth others anonymously like spoiled child. You are not only throwing dirt on such a prestigious Indian institution, and also on many decent and capable scientists who do outstanding work and produce brilliant graduate students, some of which I was fortunate to have in my lab. ** ** Best Narayana Sthanam ** ** *--* *Narayana Sthanam,Ph D* *Professor of Structural Biology* *244 CBSE 1025 18th Street South* *Center for Biophysical Sciences and Engineering* *University of Alabama at Birmingham* *Birmingham, Al 35294* *Phone: 205 934 0119* *URL: **http://www.opt.uab.edu/narayanalab*https://mail.ad.uab.edu/owa/redir.aspx?C=80a7a9ef03ea46b58d648b78f13d4272URL=http%3a%2f%2fwww.opt.uab.edu%2fnarayanalab “Never let success go to your head, nor failure to your heart” ** ** ** ** *From:* CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] *On Behalf Of *U US *Sent:* Monday, October 29, 2012 12:03 PM *To:* CCP4BB@JISCMAIL.AC.UK *Subject:* Re: [ccp4bb] Faculty positions at Molecular Biophysics Unit, IISc, Bangalore ** ** Dear Friends, There is no need to apply to this position, we suggest. It is a PREDETERMINED SELECTION, i.e. candidate is fixed and this (advertisement, screening, selection board, selection and approval) is just the procedure. It does not matter whether you apply or not. If you apply and called for interview, then you have to waste your valuable time as well as huge travel money unless some Big Boss is fixing you to the post. Interestingly Indian Institute of Science recruits and carries faculties and trains them in such a way that it has become a epicentre of recruitment scams across India and it make rest of Indian Scientists/Faculties in their path of scams and CRIME. Students also inherit the character of their boss. They do not participate in any form of fair selection in the country. Almost all cases they select and load many times inferior candidates even though candidate was not seen by anybody or interviewed. Similarly they distribute various national awards among themselves and within their group. THEY ARE NOT ASHAMED AT ALL. This is just an attempt of WASTING HUGE PUBLIC MONEY by a bunch of crooks who are good for nothing but worst for everything. Sham ** ** -- Date: Mon, 29 Oct 2012 11:47:06 +0530 From: vikasnavra...@gmail.com Subject: [ccp4bb] Faculty positions at Molecular Biophysics Unit, IISc, Bangalore To: CCP4BB@JISCMAIL.AC.UK Dear all Kindly make a note. Indian Institute of Science, Bangalore. ** ** Molecular Biophysics Unit (http://mbu.iisc.ernet.in/) ** ** Opening for Assistant Professor Positions. ** ** Applicants, preferably below 35 years, should have a Ph.D. with postdoctoral experience with an excellent publication record. ** ** We seek candidates in the general area of structural biology with an emphasis on understanding macromolecular systems at the molecular level. ** ** Applications with a detailed CV, research plan (not exceeding 3 pages) and names of at least 4 referees should be sent to
Re: [ccp4bb] Faculty positions at Molecular Biophysics Unit, IISc, Bangalore
I have no idea about Bangalore, but I know from personal experience that already-filled job ads exist and waste everybody's time. One of the junior faculty jobs I intereviewed for in the 90s at a prestigious US medical school turned out to be just such a thing - before I went people in the know told me which inside post-doc would get the position, faculty turnout to my presentation was low, and even during the visit somebody told me they thought it would be an inside deal. And in the end, it was. I had much better things to do with my time. ++ Phoebe A. Rice Dept. of Biochemistry Molecular Biology The University of Chicago 773 834 1723; pr...@uchicago.edumailto:pr...@uchicago.edu http://bmb.bsd.uchicago.edu/Faculty_and_Research/ http://www.rsc.org/shop/books/2008/9780854042722.asp From: CCP4 bulletin board [CCP4BB@JISCMAIL.AC.UK] on behalf of Manoj Tiwari [diffracti...@gmail.com] Sent: Monday, October 29, 2012 4:28 PM To: CCP4BB@JISCMAIL.AC.UK Subject: Re: [ccp4bb] Faculty positions at Molecular Biophysics Unit, IISc, Bangalore Just to let Narayan Viswam know more about HM Krishnamurthy. He did his PhD from IISc long long back in 1979 on small molecule crystallography. Then he did his postdoc in protein crystallography from two top most Universities of USA. He had been mentored by really really great protein crystallographers in USA. Everybody will agree that neither the Institute nor the mentors should be blamed for such acts. This is individual's character and act. I am sure that there are many more frustrated people who did not get through the prestigious institute at some level of their life. Such comments deserve nothing more than the ignorance. Seems grapes are sour :) MT On Tue, Oct 30, 2012 at 1:08 AM, Narayan Viswam nvisw...@gmail.commailto:nvisw...@gmail.com wrote: Sham is very right about the institute. Narayana Sthanam's illustrious former colleague Krishna Murthy who faked so many structures retracted quite a few papers was an alumnus of the institute. On Mon, Oct 29, 2012 at 12:21 PM, James Stroud xtald...@gmail.commailto:xtald...@gmail.com wrote: The agism in the advertisement doesn't do the institute much credit. I'm inclined to believe Sham, given the Institutes stated policies: Applicants, preferably below 35 years James On Oct 29, 2012, at 11:28 AM, Narayana VL Sthanam wrote: Sham, who so ever you are, if you have such a long list of complaints, why don’t you put your name clearly and complain openly, instead of hiding behind some anonymous ‘SHAM’ name. What you write about IISc may be all true or it may be reflection of your frustration for not getting a job at IISc! How do we know? So, grow a ‘spine’, if you have a complaint, say it like a man and do not hide behind and bad mouth others anonymously like spoiled child. You are not only throwing dirt on such a prestigious Indian institution, and also on many decent and capable scientists who do outstanding work and produce brilliant graduate students, some of which I was fortunate to have in my lab. Best Narayana Sthanam -- Narayana Sthanam,Ph D Professor of Structural Biology 244 CBSE 1025 18th Street South Center for Biophysical Sciences and Engineering University of Alabama at Birmingham Birmingham, Al 35294 Phone: 205 934 0119tel:205%20934%200119 URL: http://www.opt.uab.edu/narayanalabhttps://mail.ad.uab.edu/owa/redir.aspx?C=80a7a9ef03ea46b58d648b78f13d4272URL=http%3a%2f%2fwww.opt.uab.edu%2fnarayanalab “Never let success go to your head, nor failure to your heart” From: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UKmailto:CCP4BB@JISCMAIL.AC.UK] On Behalf Of U US Sent: Monday, October 29, 2012 12:03 PM To: CCP4BB@JISCMAIL.AC.UKmailto:CCP4BB@JISCMAIL.AC.UK Subject: Re: [ccp4bb] Faculty positions at Molecular Biophysics Unit, IISc, Bangalore Dear Friends, There is no need to apply to this position, we suggest. It is a PREDETERMINED SELECTION, i.e. candidate is fixed and this (advertisement, screening, selection board, selection and approval) is just the procedure. It does not matter whether you apply or not. If you apply and called for interview, then you have to waste your valuable time as well as huge travel money unless some Big Boss is fixing you to the post. Interestingly Indian Institute of Science recruits and carries faculties and trains them in such a way that it has become a epicentre of recruitment scams across India and it make rest of Indian Scientists/Faculties in their path of scams and CRIME. Students also inherit the character of their boss. They do not participate in any form of fair selection in the country. Almost all cases they select and load many times inferior candidates even though candidate was not seen by anybody or interviewed. Similarly they distribute various national awards among themselves and within
Re: [ccp4bb] Faculty positions at Molecular Biophysics Unit, IISc, Bangalore
Dear Patrick, Thanks and Nice query. As stated, Almost all cases they select and load many times inferior candidates even though candidate was not seen by anybody (selectors) or interviewed. This means all what they are. In all cases it is their mutual understanding to fix their own people, through the back-door deal. Here those people will disagree who got into a position through the same means. Some scientists are excellent in science, and some students are really genius. Purpose is not to defame Indian Institute of Science (IISc). But there have been enormous number of garbage loaded who are not only defaming IISc but also entire India. Unfortunately they are heading and dominating entire India with some of their supporting partners. If Faculties/Scientists of this Institute of repute do not stand bold against the wrong doing and illegal activities within the Institute and outside where they are called for as a selector but actively participate in all shorts of illegal activities including closed room deal, then those elements needed to be cleaned. They are not only destroying repute of IISc, but also entire system including scientific community and outcome (Cheating, retraction, plagiarism and so on). World suffers not because of violence of the bad people, but because of silence of good people. If good people remain silent during the crisis then they cannot claim as good people. Formation of Good crystal depends on formation of good nucleation. Good crystal gives good diffraction and good structure in the end. In entire India (IISERs, IITs, NIPERs, NITs, Universities) substandard people has been loaded by the substandard people acting as a selector and IISc takes its leading role. All through the back door deal and predetermined. So quality of diffraction (i.e. outcome) one can easily imagine. India is probably going though the worst kind of regime of scams (many trillions $) and lowest moral. In India there is nothing called fair selection, all are predetermined. Any kind of predetermined execution is nothing but crime - be it in Wall Street, London, or Europe and any other civilized place. If they have a better or best candidate of their own choice, they should not do all the drama of advertisement, screening, selection board, selection and approval by the selection committee etc. wasting huge public money. Moreover, they do not pay travel or accommodation cost in general to the candidate(s). Because if candidates do not appear, selection is fixed; if candidates appear, then also selection is fixed. Age restriction only applies to those who do not have back up of big boss. If they want to feed somebody they will do at any age. If age factor is meaningful then retired person should not get the extension. But there are Old-homes for which public expenditure is enormous without much outcome. Therefore, bureaucracy is of worst kind. So, no hope of natural evolution of talents, though they deliver fake speeches. Again, India is probably going though the worst kind of regime of scams (many trillions $) and lowest moral (Political as well as social). In stead of being emotional and attacking us, IISc alumni must come forward, unitedly address the core issues (stated) for the goodness of every Indians and scientific community overall. You will not do that, because you all like to enjoy the wine from the same bottle your bosses made for you. Students also inherit the character of their boss. We did not post anything against the Inst., we just made people aware that there is not nothing in India which is not predetermined selection. Thanks.Sham Date: Mon, 29 Oct 2012 19:00:50 + Subject: Re: [ccp4bb] Faculty positions at Molecular Biophysics Unit, IISc, Bangalore From: patr...@douglas.co.uk To: u...@hotmail.com Dear Sham Out of curiosity, why do you say all that? What you say may be true, but how can you tell? Do they always take their own people? Maybe they have someone in mind, but maybe they will change their minds if someone even better turns up. Just interested. Patrick On 29 October 2012 17:03, U US u...@hotmail.com wrote: Dear Friends, There is no need to apply to this position, we suggest. It is a PREDETERMINED SELECTION, i.e. candidate is fixed and this (advertisement, screening, selection board, selection and approval) is just the procedure. It does not matter whether you apply or not. If you apply and called for interview, then you have to waste your valuable time as well as huge travel money unless some Big Boss is fixing you to the post. Interestingly Indian Institute of Science recruits and carries faculties and trains them in such a way that it has become a epicentre of recruitment scams across India and it make rest of Indian Scientists/Faculties in their path of scams and CRIME. Students also inherit the character of their boss. They do not participate in any form of fair selection in the country. Almost all cases
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Dear CCP4 users, It is extremely sad that CCP4BB has failed to moderate/screen for such spam mails! Thanks kavya Dear Friends, There is no need to apply to this position, we suggest. It is a PREDETERMINED SELECTION, i.e. candidate is fixed and this (advertisement, screening, selection board, selection and approval) is just the procedure. It does not matter whether you apply or not. If you apply and called for interview, then you have to waste your valuable time as well as huge travel money unless some Big Boss is fixing you to the post. Interestingly Indian Institute of Science recruits and carries faculties and trains them in such a way that it has become a epicentre of recruitment scams across India and it make rest of Indian Scientists/Faculties in their path of scams and CRIME. Students also inherit the character of their boss. They do not participate in any form of fair selection in the country. Almost all cases they select and load many times inferior candidates even though candidate was not seen by anybody or interviewed. Similarly they distribute various national awards among themselves and within their group. THEY ARE NOT ASHAMED AT ALL. This is just an attempt of WASTING HUGE PUBLIC MONEY by a bunch of crooks who are good for nothing but worst for everything. Sham -- This message has been scanned for viruses and dangerous content by MailScanner, and is believed to be clean.
