[ccp4bb] Position available: Protein biochemist at Civetta Therapeutics

2021-01-12 Thread Mike S 
https://careers-civetta.icims.com/jobs/1395/job

Overview

Civetta Therapeutics (Cambridge, MA) is recruiting a Scientist to Senior
Scientist level position in Protein Sciences. Civetta is a cutting-edge
biotechnology company, backed by Deerfield Management, and recently
launched from the Broad Institute and Dana-Farber Cancer Institute (DFCI).
Our founders, William Sellers (Broad/DFCI) and Eric Fischer (DFCI), world
leaders in genomics and protein biology, launched Civetta with the mission
to create a new precision medicine platform targeting “propeller” proteins.

We are building a team of talented, dedicated scientists who share our
vision and our passion to discover groundbreaking new drugs to treat some
of the most difficult diseases, including cancer.  If you are a talented
life sciences professional who brings energy, passion, and scientific
curiosity to the team, we welcome the opportunity to consider you for this
important position in our growing company.



*The position.*  We are seeking an experienced and creative protein
biochemist at the Scientist to Senior Scientist level to propel our
gene-to-structure (G2S) efforts in Protein Sciences, helping to build
world-class platform capabilities in discovering small molecule modulators
of beta-propeller proteins, supporting biological discovery and our drug
development programs.
Responsibilities

Key Responsibilities

   - Provide expertise and insight for construct design and purification
   protocols for protein production.
   - Perform purification of challenging beta-propeller proteins to
   establish robust protocols that can be used internally or transferred to
   external (CRO) resources as needed.
   - Specify and procure necessary equipment, enabling technologies and
   reagents.
   - Collaborate productively with biochemistry and biophysics colleagues
   to source protein reagents for assay development targeting beta-propeller
   proteins and multi-protein complexes.
   - Make intellectual contributions to all stages of drug discovery and
   project delivery.

Qualifications

Qualifications

   - Ph.D. degree in biochemistry, structural biology or related discipline
   with 5+ years of industry experience or Master’s/Bachelor’s degree with 10+
   years of experience.
   - Extensive protein biochemistry experience working with challenging
   proteins and multi-protein complexes.
   - Experience using specialized purification strategies, such as HIC and
   protein refolding.
   - Knowledge of protein structure and experience in the preparation of
   high-quality, concentrated protein samples for biophysical (SPR, ITC, DSF)
   and structural studies (NMR, X-ray, Cryo-EM).
   - Experience balancing CRO and internal resources to solve problems and
   drive progress.
   - Exceptional interpersonal and communication skills, and ability to
   effectively present data within the company and to other stakeholders.
   - Adept at working effectively within motivated cross-functional
   scientific teams to deliver project goals within timelines.


Civetta Therapeutics provides equal employment opportunities (EEO) to all
employees and applicants for employment without regard to religion, race,
creed, color, sex, sexual orientation, alienage or citizenship status,
national origin, age, marital status, pregnancy, disability, veteran or
military status, predisposing genetic characteristics or any other
characteristic protected by applicable federal, state or local law.



*Notice to Third-Party Recruiters/Staffing Agencies:* Recruitment is
managed through Civetta Therapeutics’ Human Resources department. Resumes
will only be accepted from staffing agency/recruiters if there is a signed
contract in place. Recruiters are requested to not contact our hiring
managers or employees directly to inquire about open positions or to
present candidates. In the event a staffing agency/third-party recruiter
submits a resume without a contract in place, the candidate submitted will
be considered unsolicited and treated as if the candidate submitted their
resume directly to Civetta, and no fee/payment will be paid. Recruiters
interested in working with Civetta Therapeutics can submit their
information to cont...@civettatherapeutics.com, and we will contact you if
needed.



PM18

#LI-DNI



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Re: [ccp4bb] Data 3 A

2021-01-12 Thread Ethan A Merritt 
On Tuesday, 12 January 2021 15:22:18 PST rohit kumar wrote:
> Dear all,
> 
> I am trying to solve a data with 3 A resolution, however data quality is
> very bad and mathews coffi. suggest  two molecules per ASU but It always
> gives one molecule in AU after phaser with the TFZ and RFZ score are 4.5
> and 3.5 respectively with LLG gain 121.
> And when I used that model for Refmac the final R/Rfee is 23/30 and with
> satisfactory Ramachandran statistics as well as electron density and model
> in agreement with each other  in coot. Does It mean that I have
> correct solution?

Sounds promising.
The other to check is that the model you have exhibits a set of lattice
contacts sufficient to form a believable crystal packing.  If the 
symmetry-related
molecules do not touch each other then there must be some component
missing from your model.

cheers,

Ethan


-- 
Ethan A Merritt
Biomolecular Structure Center,  K-428 Health Sciences Bldg
MS 357742,   University of Washington, Seattle 98195-7742



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Re: [ccp4bb] Data 3 A

2021-01-12 Thread Pascal Egea 
Hi,
Have you looked at the packing of your crystal with that single molecule in
the ASU. Is it enough to build a crystal as in symmetry related mates
contact each other  (with probably quite a bit a solvent) but still a
crystal.
That would not be shocking since your resolution is average (on offense
intended).

Best,
Pascal Egea

On Tue, Jan 12, 2021 at 3:22 PM rohit kumar  wrote:

> Dear all,
>
> I am trying to solve a data with 3 A resolution, however data quality is
> very bad and mathews coffi. suggest  two molecules per ASU but It always
> gives one molecule in AU after phaser with the TFZ and RFZ score are 4.5
> and 3.5 respectively with LLG gain 121.
> And when I used that model for Refmac the final R/Rfee is 23/30 and with
> satisfactory Ramachandran statistics as well as electron density and model
> in agreement with each other  in coot. Does It mean that I have
> correct solution?
> Please suggest,
>
> --
> Regards
> Dr. Rohit Kumar Singh
> Postdoctoral fellow
>
>
>
> --
>
> To unsubscribe from the CCP4BB list, click the following link:
> https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB=1
>
-- 
Pascal F. Egea, PhD
Associate Project Scientist
UCLA, David Geffen School of Medicine
Department of Biological Chemistry
Boyer Hall room 356
611 Charles E Young Drive East
Los Angeles CA 90095
office (310)-983-3515
lab  (310)-983-3516
email pegea at mednet.ucla.edu



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[ccp4bb] Data 3 A

2021-01-12 Thread rohit kumar 
Dear all,

I am trying to solve a data with 3 A resolution, however data quality is
very bad and mathews coffi. suggest  two molecules per ASU but It always
gives one molecule in AU after phaser with the TFZ and RFZ score are 4.5
and 3.5 respectively with LLG gain 121.
And when I used that model for Refmac the final R/Rfee is 23/30 and with
satisfactory Ramachandran statistics as well as electron density and model
in agreement with each other  in coot. Does It mean that I have
correct solution?
Please suggest,

-- 
Regards
Dr. Rohit Kumar Singh
Postdoctoral fellow



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[ccp4bb] Postdoc position in computational modeling at Rice U (special fellowship opportunity if apply by this weekend)

2021-01-12 Thread George Lu 

Dear colleagues,

We are recruiting a postdoc on protein modeling and design direction, 
and if you *apply by this weekend*, we may have time to prepare an 
application for the Rice University Academy of Fellows, which will boost 
your stipend to 60,000 per year. See below for details:




The Lab for Synthetic Macromolecular Assemblies  
(PI: George J. Lu) at Rice Bioengineering is recruiting a postdoc fellow 
with expertise on *protein structure modeling and bioinformatics*. Wet 
lab experience in molecular biology and biochemistry will be a bonus. 
The recruited postdoc will lead projects in designing and evolving novel 
genetic variants of protein nanostructures, and *have opportunities to 
learn a multidisciplinary skillset *including synthetic biology, 
directed evolution and small-animal imaging.


The research in the lab focuses on a class of gas-filled protein 
nanostructures named gas vesicles, and use them to develop novel imaging 
and cellular control technologies for basic research, cell-based 
therapies, and biomaterials. Gas vesicles were recently shown to enable 
ultrasound- and MRI-based imaging of gene expressions in therapeutic 
microbes and mammalian cells Nature Materials 2018 
; Nature Protocols 2017 
; Nature 2018 
; Science 2019 
), and have potential 
applications in remote cellular control and biomaterials.


Dr. Lu was previously awarded the Young Investigator of the Year from 
the World Molecular Imaging Society for his work on the development of 
acoustically erasable MRI reporter genes. The independent lab was newly 
established in 2020 with support from the *NIH Pathway-to-independence* 
(K99/R00) and the *Cancer Prevention and Research Institute of Texas 
(CPRIT) *Scholar awards.


*Rice Bioengineering* is one of the top biomedical engineering 
departments with its graduate program consistently *ranked among the top 
10 in the country*. The Texas Medical Center across the street is the 
largest medical center in the continent with multiple leading medical 
institutes such as MD Anderson, Baylor College of Medicine and UT 
Health. The candidate will have opportunities to lead cutting-edge 
research projects and interact with outstanding scientists as 
collaborators and trainees. The candidate should have strong written and 
oral communication skills, and will also be expected to aid in the 
supervision of junior lab members and the preparation of research proposals.


The pay scale for the position follows the university guidelines. Please 
note if *applied and interviewed before Feb.1*, the selected candidate 
will be eligible to apply to Rice University Academy of Fellows 
, which will *boost the stipend to 60,000 
per year*.


Rice University is an Equal Opportunity Employer with commitment to 
diversity at all levels, and considers for employment qualified 
applicants without regard to race, color, religion, age, sex, sexual 
orientation, gender identity, national or ethnic origin, genetic 
information, disability, or protected veteran status. To apply, please 
email a cover letter and a copy of CV/resume to george...@rice.edu 
.




Best,
George


George J. Lu, PhD
Assistant Professor of Bioengineering
CPRIT Scholar in Cancer Research
Rice University
http://lulab.rice.edu 






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[ccp4bb] iNEXT-Discovery 2021 webinars

2021-01-12 Thread Anastassis Perrakis 
Dear all,

I do hope that after a full week for recovery from the CCP4 “study weekend”, 
you might want to consider joining our iNEXT-Discovery webinar series! We have 
lined up a series of new talks on X-ray tomography, cryo-EM, NMR, and more!

To see the full program and also register (even if you registered for the 2020 
series, you need to register again!) at :

https://zoom.us/webinar/register/4016104403708/WN_Ofbx1MrNS-OAK95yMbtmXA

We hope to see many of you there, and wanted to also remind you that you are 
more than welcome to apply for access for X-rays (SAXS, MX, fragment and 
ligands screens, tomography), cryo-EM (single particles, tomography, FIB, etc), 
NMR (solution, solid, fragment screens, etc, and macromolecular biophysics 
(SPR, ITC, MST, FP, Stopped Flow, etc) at any time through the iNEXT-Discovery 
web site:

https://inext-discovery.eu/network/inext-d/home

Best regards,

Tassos











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