[ccp4bb] QC Server (was Re: [ccp4bb] Another paper structure retracted)
I think you'll find it works better if you use Fasta format: http://en.wikipedia.org/wiki/FASTA_format Chris From: Phil Evans p...@mrc-lmb.cam.ac.uk To: CCP4BB@JISCMAIL.AC.UK Date: 08/12/2011 01:16 AM Subject: Re: [ccp4bb] Another paper structure retracted Sent by: CCP4 bulletin board CCP4BB@JISCMAIL.AC.UK Can anyone get this server to work? For me it keeps complaining that my sequence file is not a PIR file. The file looks OK to me, but I've never really understood what a PIR file is Phil On 12 Aug 2011, at 01:39, Kevin Jin wrote: Should we really have some crystallographers to review and qc those structures before the formal releasing? JCSG has set a very good mechanism for this issue. There is a sever for self check. http://smb.slac.stanford.edu/jcsg/QC/ ___ This is an e-mail from Danisco and may contain confidential information. If you are not the intended recipient and you receive this e-mail by mistake, you are not allowed to use the information, to copy it or distribute it further. Please notify us and return it to Danisco by e-mail and delete all attachments. Thank you for your assistance. __
[ccp4bb] heterologous seeding
Hi, I seem to recall a paper from a few years back that used heterologous proteins for macro/micro seeding, and of course now I can't find it. Seems likely that lysozyme would be involved... I've found plenty on using non-protein compounds as seeds, but nothing for protein. Does anyone know if such a reference is out there? Thanks! Chris ___ This is an e-mail from Danisco and may contain confidential information. If you are not the intended recipient and you receive this e-mail by mistake, you are not allowed to use the information, to copy it or distribute it further. Please notify us and return it to Danisco by e-mail and delete all attachments. Thank you for your assistance. __
[ccp4bb] anaerobic crystallization
Hi, Does anyone have tips or suggestions for getting started with anaerobic crystallization? Searching through the archives, I was able to find some discussion about various glove box options (http://tinyurl.com/ccp4-glovebox), but not about the process itself (we already have a box). To simplify things, would it be worthwhile to perform the initial screens in something like the pre-filled Qiagen screens (http://tinyurl.com/qiagen-xseal)? Do I need to worry about evaporation while solutions are being degassed (esp volatiles)? Better/easier to try microbatch? Thanks for any input. Chris ___ This is an e-mail from Danisco and may contain confidential information. If you are not the intended recipient and you receive this e-mail by mistake, you are not allowed to use the information, to copy it or distribute it further. Please notify us and return it to Danisco by e-mail and delete all attachments. Thank you for your assistance. __
Re: [ccp4bb] desktop models- summary
Thanks very much for the replies. Here is a list of resources: http://www.bathsheba.com/ http://www.rpc.msoe.edu/cbm/ http://www.crystalprotein.com/ http://www.bioetch.com/ http://www.luminorum.com/ http://bioetch.com/ And if you want to roll your own: http://www.rap-man.com/ http://homemade3dprinter.blogspot.com/ Chris -Original Message- From: CCP4 bulletin board [mailto:ccp...@jiscmail.ac.uk] On Behalf Of Christopher Rife Sent: Tuesday, May 05, 2009 2:42 PM To: CCP4BB@JISCMAIL.AC.UK Subject: [ccp4bb] desktop models Hi, I am looking to have a model produced from a PDB, i.e. something that we can put on the desk for everyone to admire :) Googling for such a thing is rather challenging, so I'm curious if anyone has suggestions as to where I might get something like that made. So far, I have found two options: 1. Molecular Dimensions: http://www.moleculardimensions.com/us/merchant.ihtml?new_id=223step=2 (etching in a glass block) 2. 3D Molecular Designs: http://www.3dmoleculardesigns.com/ (nylon models) I think #2 is more what we have in mind. Does anyone have any experience with their final product in terms of quality and durability? Thanks very much. Chris ___ This is an e-mail from Danisco and may contain confidential information. If you are not the intended recipient and you receive this e-mail by mistake, you are not allowed to use the information, to copy it or distribute it further. Please notify us and return it to Danisco by e-mail and delete all attachments. Thank you for your assistance. __
[ccp4bb] desktop models
Hi, I am looking to have a model produced from a PDB, i.e. something that we can put on the desk for everyone to admire :) Googling for such a thing is rather challenging, so I'm curious if anyone has suggestions as to where I might get something like that made. So far, I have found two options: 1. Molecular Dimensions: http://www.moleculardimensions.com/us/merchant.ihtml?new_id=223step=2 (etching in a glass block) 2. 3D Molecular Designs: http://www.3dmoleculardesigns.com/ (nylon models) I think #2 is more what we have in mind. Does anyone have any experience with their final product in terms of quality and durability? Thanks very much. Chris ___ This is an e-mail from Danisco and may contain confidential information. If you are not the intended recipient and you receive this e-mail by mistake, you are not allowed to use the information, to copy it or distribute it further. Please notify us and return it to Danisco by e-mail and delete all attachments. Thank you for your assistance. __
