[ccp4bb] Beam time available now at MacCHESS

[Doletha Marian Szebenyi]

From: Doletha Marian Szebenyi 
Sent: Wednesday, April 14, 2021 10:12 AM
To: ccp4bb@jiscmail.ac.uk 
Subject: Beam time available now at MacCHESS


Rapid access beam time for macromolecular crystallography is available now at:
[cid:324d0976-5c56-4ddf-884f-4ec63b50ac92]
Features include:

  *   EIGER2 16M detector
  *   Automounter accepting MSC pucks, Unipucks, Rigaku pucks (loaner kit with 
MSC pucks available)
  *   100 µ standard beam or 15 µ minibeam (focused with CRL)
  *   Full 24-hr support for remote or in-person operation
  *   Cryo or room-temperature data collection
  *   High-pressure cryocooling option to improve diffraction quality or trap 
gases
  *   Serial crystallography option (fixed-target)
  *   High-pressure data collection in diamond anvil cell

Visit the CHESS User Portal<https://userdb.chess.cornell.edu> to request beam 
time. For more information, visit our web 
site<https://www.chess.cornell.edu/macchess> or contact me (dm...@cornell.edu).

Marian Szebenyi
Director, MacCHESS



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[ccp4bb] Staff scientist position at CHESS

[Doletha Marian Szebenyi]

We are still accepting applications for the staff scientist position described 
below. Here's a chance to work at a beamline that specializes in non-standard 
experiments, and to contribute to deciding what direction development at 
MacCHESS will take over the next few years.

Staff Scientist position at MacCHESS

The Macromolecular Diffraction Facility of the Cornell High-Energy Synchrotron 
Source (MacCHESS) has an opening for a Staff Scientist (Research Associate) to 
run the Flexible Crystallography (FlexX) station. Duties include both support 
of users performing routine macromolecular crystallography (MX) and development 
of novel techniques in X-ray scattering as applied to structural
biology. User support includes continuing developments to maintain a 
state-of-the-art facility. Novel techniques are developed in collaboration with 
scientists who need capabilities beyond the current standard methods. Areas of 
particular interest are crystallography at (1) ambient temperature, including 
serial techniques, and (2) high pressure, in a diamond anvil cell (DAC) or 
using the high pressure cryocooling technique, especially for the trapping of 
intermediate states of reactions in crystallo.

Applicants should have a Ph.D. in a relevant field (biophysics, structural 
biology etc.), and at least 3 years of experience beyond the degree.
Preference will be given to those with experience with methods related to X-ray 
scattering experiments, and to those who have worked at a synchrotron facility. 
Good clear communication skills are a must, including fluency in the English
language.

Appointments are nominally for three years and are renewable, contingent upon 
availability of funds and employee performance.

Located on an Ivy League university campus in picturesque upstate New York, the 
Cornell High-Energy Synchrotron Source (CHESS) serves a world-wide user base of 
structural biologists, chemists, physicists, and engineers. MacCHESS is an 
NIH-supported National Resource providing support for structural biology at 
CHESS.

Applications should be submitted at http://academicjobsonline.org/ (posting 
#20601) and should include a cover letter, a CV, a list of
publications, and a detailed summary of research experience and interests. 
Please arrange to have at least three letters of recommendation sent, as per 
instructions on the academicjobsonline website. The starting date is negotiable.
For more information about the position, see 
https://www.chess.cornell.edu/macchess-staff-scientist.

Cornell University requires all employees to be fully vaccinated against 
COVID-19, or to have obtained a university-approved medical or religious
exemption.

Diversity and Inclusion are a part of Cornell University’s heritage. We’re a 
recognized employer and educator valuing AA/EEO, Protected Veterans, and 
Individuals with Disabilities.



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https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB=1

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[ccp4bb] Director of MacCHESS facility

[Doletha Marian Szebenyi]

The Macromolecular Diffraction Facility of the Cornell High-Energy Synchrotron 
Source (MacCHESS)is seeking a Director. MacCHESS is a component of CHESS, a 
recently-upgraded synchrotron source serving a wide variety of researchers with 
X-ray science needs, jointly funded by NIH, NSF, and DOD, with assistance from 
New York State. MacCHESS provides a facility where scientists can use both 
established and emerging technologies to pursue the goals of the biomedical 
research community. Work performed at MacCHESS, using tools including, e.g., 
crystallography and small-angle X-ray scattering, yields important insights 
into fundamental biological processes. The MacCHESS Director will play a key 
role, alongside the MacCHESS PI and other members of the CHESS leadership team, 
in the management of the CHESS facility to promote scientific research.

The primary functions of the MacCHESS Director are (1) to ensure smooth 
operation of the facility (personnel management, budgeting, upgrades), (2) to
act as Co-PI on the NIH grant (preparing reports, renewal applications, etc.), 
and (3) to participate in collaborative scientific developments at MacCHESS.

Applicants should have a Ph.D. in a relevant field (biophysics, structural 
biology etc.), and experience managing a group of mixed personnel (scientific,
technical, administrative. Experience with managing grants (writing 
applications, preparing reports, etc.) from federal agencies is highly 
desirable. Experience at a user facility is a plus, as is expertise in methods 
related to X-ray science. Good clear communication skills are a must, including 
fluency in the English language.

Located on an Ivy League university campus in picturesque upstate New York, the 
Cornell High-Energy Synchrotron Source (CHESS) serves a world-wide user base of 
structural biologists, chemists, physicists, and engineers. MacCHESS is an 
NIH-supported National Resource providing support for structural biology at 
CHESS.

Applications should be submitted at http://academicjobsonline.org/ (posting 
#20602) and should include a cover letter, a CV, a list of publications, and a 
detailed summary of research experience and interests. Please arrange to have 
at least three letters of recommendation sent, as per instructions on the 
academicjobsonline website. The starting date is negotiable. For more 
information about the position, see 
https://www.chess.cornell.edu/macchess-program-director-senior-research-associate.

Cornell University requires all employees, to be fully vaccinated against 
COVID-19, or to have obtained a university-approved medical or religious 
exemption.

Diversity and Inclusion are a part of Cornell University’s heritage. We’re a 
recognized employer and educator valuing AA/EEO, Protected Veterans, and 
Individuals with Disabilities.



To unsubscribe from the CCP4BB list, click the following link:
https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB=1

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[ccp4bb] Beamline Scientist position at MacCHESS

[Doletha Marian Szebenyi]

The Macromolecular Diffraction Facility of the Cornell High-Energy Synchrotron 
Source (MacCHESS) has an opening for a Staff Scientist (Research Associate) to 
run the Flexible Crystallography (FlexX) station. Duties include both support 
of users performing routine macromolecular crystallography (MX) and development 
of novel techniques in X-ray scattering as applied to structural biology. User 
support includes continuing developments to maintain a
state-of-the-art facility. Novel techniques are developed in collaboration with 
scientists who need capabilities beyond the current standard methods. Areas of 
particular interest are crystallography at (1) ambient temperature, including 
serial techniques, and (2) high pressure, in a diamond anvil cell (DAC) or
using the high pressure cryocooling technique, especially for the trapping of 
intermediate states of reactions in crystallo.

Applicants should have a Ph.D. in a relevant field (biophysics, structural 
biology etc.), and at least 3 years of experience beyond the degree. Preference 
will be given to those with experience with methods related to X-ray scattering 
experiments, and to those who have worked at a synchrotron facility. Good clear 
communication skills are a must, including fluency in the English language.

Appointments are nominally for three years and are renewable, contingent upon 
availability of funds and employee performance.

Located on an Ivy League university campus in picturesque upstate New York, the 
Cornell High-Energy Synchrotron Source (CHESS) serves a world-wide user base of 
structural biologists, chemists, physicists, and engineers. MacCHESS is an 
NIH-supported National Resource providing support for structural biology at 
CHESS.

Applications should be submitted at http://academicjobsonline.org/ (posting 
#20601) and should include a cover letter, a CV, a list of
publications, and a detailed summary of research experience and interests. 
Please arrange to have at least three letters of recommendation sent, as per
instructions on the academicjobsonline website. The starting date is 
negotiable. For more information about the position, see
https://www.chess.cornell.edu/macchess-staff-scientist.

Cornell University requires all employees, to be fully vaccinated against 
COVID-19, or to have obtained a university-approved medical or religious
exemption.

Diversity and Inclusion are a part of Cornell University’s heritage. We’re a 
recognized employer and educator valuing AA/EEO, Protected Veterans, and
Individuals with Disabilities.



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https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB=1

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[ccp4bb] Beam time available now at MacCHESS

[Doletha Marian Szebenyi]

Rapid access beam time for macromolecular crystallography is available now at:
[cid:324d0976-5c56-4ddf-884f-4ec63b50ac92]
Features include:

  *   Recently upgraded 3rd-generation synchrotron source, CHESS-U
  *   EIGER2 16M detector
  *   Automounter accepting MSC pucks, Unipucks, Rigaku pucks (loaner kit with 
MSC pucks available)
  *   100 µ standard beam or 15 µ minibeam (focused with CRL)
  *   Remote operation using NoMachine
  *   24-hr support
  *   Cryo or room-temperature data collection
  *   High-pressure cryocooling option to improve diffraction quality
  *   Serial crystallography option (fixed-target)
  *   High-pressure data collection in diamond anvil cell under development - 
talk to us about it!

Visit the CHESS User Portal to request beam 
time. For more information, visit our web 
site or contact myself 
(dm...@cornell.edu) or Beamline Scientist Aaron Finke (af...@cornell.edu).

Marian Szebenyi
Director, MacCHESS



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[ccp4bb] Postdoc position at CHESS

[Doletha Marian Szebenyi]

The Macromolecular Diffraction and High Pressure Biology resources at the
Cornell High Energy Synchrotron Source (MacCHESS and CHEXS/HP-Bio, respectively)
have an opening for a Postdoctoral Associate to develop applications of high
pressure to answer biological questions concerning enzyme mechanisms, protein
folding, adaptation to extreme environments, and the origin of life. The
primary method to be used is pressurization of macromolecular crystals in a
diamond anvil cell (HP-MX), but other techniques such as high-pressure small
angle X-ray scattering (HP-SAXS) will also be available. A Ph.D. degree in a
relevant field (e.g. structural biology or biophysics) is required.
Experience in crystallography is highly desirable, and experience working with
samples in "non-standard" environments is a plus. The ability to identify
suitable systems for study, through researching previous work and establishing
suitable collaborations, is essential. In addition to pursuing his/her own study
of pressure effects, the successful candidate will be expected to assist
research groups seeking to use high-pressure techniques at CHESS for their work.
Good clear communication skills are a must, including fluency in the English
language. Appointments are for one year at a time and are renewable for
additional years, contingent upon availability of funds and employee
performance.

Located on an Ivy League university campus in picturesque upstate New York,
the Cornell High-Energy Synchrotron Source (CHESS) serves a world-wide user
base of structural biologists, chemists, physicists, and engineers.
MacCHESS is an NIH-supported National Resource providing support for structural
biology at CHESS, and CHEXS is an NSF-supported facility incorporating several
projects, including high-pressure biology.

Applications should be submitted at http://academicjobsonline.org/
(posting #18189) and should include a cover letter, a CV, a list of
publications, and a detailed summary of research experience and interests.
Please arrange to have at least three letters of recommendation sent, as per
instructions on the academicjobsonline website. The starting date is negotiable.
For more information about the position, contact Dr. Marian Szebenyi at
dm...@cornell.edu.

Diversity and Inclusion are a part of Cornell University’s heritage. We’re a
recognized employer and educator valuing AA/EEO, Protected Veterans, and
Individuals with Disabilities.




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https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB=1

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[ccp4bb] Beam time available at CHESS

[Doletha Marian Szebenyi]

Beamtime for MX still available at CHESS, Nov. 1 - Dec. 21, 2017


The CHESS/MacCHESS facility, located at Cornell University in Ithaca, NY, 
invites macromolecular crystallographers to apply for time on our F1 station.

This station is well equipped for crystallographic data collection, for MR or 
Se SAD phasing. It features a Dectris Pilatus3 6M detector and an ALS-type 
automounter. We offer remote and mail-in modes of data collection, and our 
expert staff provides exceptional support 24/7. "Non-standard" experiments 
needing some special setup are welcome.

Apply for time on-line at http://userdb.chess.cornell.edu. Applications are 
accepted at any time, and turn-around time can be just a couple of weeks - less 
if you already have a proposal in our system and just need to submit a beamtime 
request.

For more information see the web site http://www.chess.cornell.edu, or contact 
administrator Kathy Dedrick, k...@cornell.edu.

= A special option ==

Pressure cryocooling of (unfrozen) crystals is available by prearrangement. 
This method can reduce the damage induced by cryocooling, often with no need 
for cryoprotectants (Kim et al., Acta Cryst. D61, 881-890 (2005)). In some 
cases, it can reduce initial disorder (high mosaicity, anisotropic diffraction, 
some kinds of twinning). Contact Marian Szebenyi (dm...@cornell.edu) if you 
have questions about pressure cryocooling.

Re: [ccp4bb] data reduction

[Doletha Marian Szebenyi]

Alex,

With regard to scalepack, be sure you use NO MERGE ORIGINAL INDEX, not
just NO MERGE.

Marian Szebenyi
MacCHESS

 Dear all:

 I am trying to solve a structure from apparently a hexagonal crystal.
 I indexed and scaled data  in P6 in Scalepack (with merging) then used
 Scalepack2mtz (with ensure unique reflections and add Rfree as well as
 the truncate procedure), and then attempted to run molecular
 replacement with Phaser. Now the problem appeared - Phaser immediately
 quits with the following error message FATAL RUNTIME ERROR:
 Reflections are not a unique set by symmetry. I do not understand
 this at all.

 I also tried running scalepack using the NO MERGE macro as people have
 indicated earlier on this bb (thank you again!, I also checked the
 scl.in that is written out and it had the NO MERGE statement), and
 then tried to run pointless to verify the spacegroup but the program
 complained the reflections are merged (that is impossible, I checked
 the number of reflections in the unmerged and merged files and they
 were different as one would expect). I repeated the procedures several
 times and I always get the same errors. I can't make any sense of this
 and I can't move forward. Any ideas?

 Many thanks,
 Alex






 On Jul 30, 2009, at 7:03 PM, CCP4BB automatic digest system wrote:

 There are 13 messages totalling 1026 lines in this issue.

 Topics of the day:

  1. refmac failed message (2)
  2. question of extra high B factor (6)
  3. Coot:findwaters in REFMAC (2)
  4. Postdoctoral position in protein crystallography at Karolinska
 Institutet
  5. OpenGL Stereo 3D on 120 Hz LCDs, at last!
  6. Foils for energy calibration

 --

 Date:Thu, 30 Jul 2009 10:50:13 GMT
 From:Elad Binshtien ela...@bgu.ac.il
 Subject: refmac failed message

 This is a multi-part message in MIME format.


 8d5c55134e491a1d1bdd
 Content-Type: text/plain; charset=utf-8
 Content-Disposition: inline
 Content-Transfer-Encoding: quoted-printable

 Dear all=2C


 I am refining a structure in Refmac at 2=2E2 A in win OS=2E=C2=A0
 Howeve=
 r=2C
 Refmac failed and send this message=3A =C2=A0 =C2=A0 =C2=A0=C2=A0
 forrtl=
 =3A error (72)=3A floating overflow=C2=A0 =


 Thank you in advance for your any helpful suggestions=2E=C2=A0 =



 Best=2C
 Elad


 Elad Binshtein
 Ph=2ED student =

 Department of Life Science =

 Ben Gurion University of the Negev
 Ph=3A 972-8-6461325=E2=80=8E

 8d5c55134e491a1d1bdd
 Content-Type: text/html; charset=utf-8
 Content-Disposition: inline
 Content-Transfer-Encoding: quoted-printable

 Dear all=2C=3Cbr=3E=3Cbr=3E=3Cbr=3EI am refining a structure in
 Refmac a=
 t 2=2E2 A in win OS=2E=26nbsp=3B However=2C
 Refmac failed and send this message=3A =26nbsp=3B =26nbsp=3B
 =26nbsp=3B=26=
 nbsp=3B forrtl=3A error (72)=3A floating overflow=26nbsp=3B
 =3Cbr=3E=3Cb=
 r=3E Thank you in advance for your any helpful
 suggestions=2E=26nbsp=3B =
 =
 3Cbr
 =3E=3Cbr=3E=3Cbr=3EBest=2C=3Cbr=3EElad=3Cbr=3E=3CBR=3E=3CBR=3EElad =
 Binshtein=3Cbr=3EPh=2ED student =3Cbr=3EDepartment of Life Science
 =3Cbr=
 =3EBen Gurion University of the Negev=3Cbr=3EPh=3A 972-8-6461325=3C/
 BR=3E=
 =3C/BR=3E=E2=80=8E

 8d5c55134e491a1d1bdd--

 --

 Date:Thu, 30 Jul 2009 13:25:43 +0100
 From:Stein, ND (Norman) norman.st...@stfc.ac.uk
 Subject: Re: refmac failed message

 This is a multi-part message in MIME format.

 --_=_NextPart_001_01CA1110.DB7CC0CB
 Content-Type: text/plain;
  charset=windows-1255
 Content-Transfer-Encoding: quoted-printable

 Hi Elad
 =20
 You don't say which version of Refmac you are using but I think the =
 first thing to do would be to try the latest version (5.5.0102). You
 can =
 pick this up from
 =20
 ftp://ftp.ccp4.ac.uk/nds/windows/refmac_5.5.0102/refmac5.exe
 =20
 Copy this exe file to the bin subdirectory of your CCP4
 installation. =
 (Probably wise to make a backup copy of the existing refmac5.exe
 first).
 =20
 Best wishes
 =20
 Norman Stein
 CCP4

 

 From: CCP4 bulletin board [mailto:ccp...@jiscmail.ac.uk] On Behalf
 Of =
 Elad Binshtien
 Sent: 30 July 2009 11:50
 To: CCP4BB@JISCMAIL.AC.UK
 Subject: [ccp4bb] refmac failed message


 Dear all,


 I am refining a structure in Refmac at 2.2 A in win OS.  However,
 Refmac =
 failed and send this message:forrtl: error (72): floating =
 overflow =20

 Thank you in advance for your any helpful suggestions. =20


 Best,
 Elad


 Elad Binshtein
 Ph.D student=20
 Department of Life Science=20
 Ben Gurion University of the Negev
 Ph: 972-8-6461325

 =FD=20

 -- =0AScanned by iCritical.=0A

 --_=_NextPart_001_01CA1110.DB7CC0CB
 Content-Type: text/html;
  charset=windows-1255
 Content-Transfer-Encoding: quoted-printable

 !DOCTYPE HTML PUBLIC -//W3C//DTD HTML 4.0 Transitional//EN
 HTMLHEAD
 META http-equiv=3DContent-Type content=3Dtext/html; =
 charset=3Dwindows-1255
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