Re: [ccp4bb] PhD position at University of Oslo
One more week to apply! On Wed, Sep 1, 2021 at 9:56 AM Nikolina Sekulic wrote: > Dear colleagues, > > > I wish to draw your attention to PhD position open in my laboratory. > > > A 3-year PhD research fellowship is available in the Group of Structural > Biology and Chromatin. > > The Group of Structural Biology and Chromatin (Sekulic group) investigates > chromatin biology and epigenetics with a biochemical and structural > approach. The project aims at understanding changes in chromatin during > mitosis and kinetochore formation. We use various biochemical and > biophysical approaches (cryo-EM, X-ray crystallography, SAXS, SANS, > hydrogen-deuterium exchange ...). In addition, we collaborate with cell > biology groups to link structure with function. > > For more information and application use the link > > > https://www.jobbnorge.no/en/available-jobs/job/211039/phd-research-fellowship-in-chromatin-and-structural-biology > > Best, > > Nikolina > > > Nikolina Sekulic, PhD > Group leader, Structural Biology and Chromatin > Centre for Molecular Medicine Norway, NCMM > Gaustadalléen 21 > Forskningsparken0349 OSLOOffice: +47 22840511 > > To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB=1 This message was issued to members of www.jiscmail.ac.uk/CCP4BB, a mailing list hosted by www.jiscmail.ac.uk, terms & conditions are available at https://www.jiscmail.ac.uk/policyandsecurity/
[ccp4bb] PhD position at University of Oslo
Dear colleagues, I wish to draw your attention to PhD position open in my laboratory. A 3-year PhD research fellowship is available in the Group of Structural Biology and Chromatin. The Group of Structural Biology and Chromatin (Sekulic group) investigates chromatin biology and epigenetics with a biochemical and structural approach. The project aims at understanding changes in chromatin during mitosis and kinetochore formation. We use various biochemical and biophysical approaches (cryo-EM, X-ray crystallography, SAXS, SANS, hydrogen-deuterium exchange ...). In addition, we collaborate with cell biology groups to link structure with function. For more information and application use the link https://www.jobbnorge.no/en/available-jobs/job/211039/phd-research-fellowship-in-chromatin-and-structural-biology Best, Nikolina Nikolina Sekulic, PhD Group leader, Structural Biology and Chromatin Centre for Molecular Medicine Norway, NCMM Gaustadalléen 21 Forskningsparken0349 OSLOOffice: +47 22840511 To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB=1 This message was issued to members of www.jiscmail.ac.uk/CCP4BB, a mailing list hosted by www.jiscmail.ac.uk, terms & conditions are available at https://www.jiscmail.ac.uk/policyandsecurity/
[ccp4bb] PhD studentship in Structural Biology and Chromatin available at University of Oslo
Dear all, I have an opening for PhD student in my group and I would be grateful if you can circulate this info between interested groups in your environment. Thanks in advance, Nikolina Nikolina Sekulic, PhD Group leader, Structural Biology and Chromatin Norwegian Center for Molecular Medicine (NCMM) Associate Professor, Department of Chemistry, University of Oslo Gaustadalléen 21 Forskningsparken 0349 OSLO Office: +47 22840511 --- PhD Research Fellowship in Structural Biology and Chromatin A 3 year PhD fellowship is available in the Group for Chromatin and Structural Biology. The Group for Structural Biology and Chromatin (Sekulic Group <http://www.med.uio.no/ncmm/english/groups/seculic-group/index.html>) investigates chromatin biology and epigenetics with a biochemical and structural approach. The project aims at understanding changes in chromatin during mitosis and kinetochore formation. We use various biochemical and biophysical approaches (X-ray crystallography, SAXS, SANS, hydrogen-deuterium exchange ...). In addition, we collaborate with cell biology groups to link structure with function. Sekulic group is affiliated with both the Faculty of Medicine and the Department of Chemistry at the Faculty of Mathematics and Natural Sciences. The PhD student will also benefit from career development programs offered both at UiO as well as the Nordic EMBL network of the Centre for Molecular Medicine, Norway. *PhD candidate*: A three-year position is available immediately. We seek highly motivated individuals with an excellent track record and experience in protein purification and biophysical and biochemical characterization of proteins and protein complexes. Experience in the field of structural biology and mass spectrometry techniques is an advantage. We offer stimulating environment with excellent working and social benefits. The purpose of the fellowship is research training leading to the successful completion of a PhD degree. The fellowship requires admission to the PhD program at the Faculty of Mathematics and Natural Sciences. The application to the PhD program must be submitted to the department no later than two months after taking up the position. For more information see: http://www.mn.uio.no/english/research/phd/ http://www.mn.uio.no/english/research/phd/application/application.html *The ideal candidate will have:* • A Master’s degree in biochemistry, chemistry, molecular biology or a related area • Hands-on experience with purification of proteins and assembly of protein complexes • Ability to independently perform experiments, analyze and interpret the results • Excellent interpersonal/communication skills • Excellent written and oral English • Ability to operate as a team player in a multi-disciplinary environment *The application must include:* • Cover Letter stating motivation and research interests • CV • Copies of certificates and grades with explanation of the grading system • A complete list of scientific publications • List of references (name, relation to candidate, e-mail and telephone number) Foreign applicants are advised to attach an explanation of their University’s grading system. Please remember that all documents should be submitted in English. In accordance with the University of Oslo's equal opportunities policy, we invite applications from all interested individuals regardless of gender or ethnicity. UiO has an agreement for all employees, aiming to secure rights to research results a.o. Note that no one can be appointed for more than one specified period at the same institution. *Salary:* The position will be placed as SKO 1017 (position code) with salary depending on qualifications within step 50-57 (NOK 436 900 – 490 900 per year) depending on qualifications and seniority. *Inquiries:* About the position: Group Leader, Nikolina Sekulic ( nikolina.seku...@biotek.uio.no). About administrative aspects of the application: Nina Modahl ( ni...@ncmm.uio.no). Please submit your application through the web-based recruitment system linked to the announcement. https://www.jobbnorge.no/en/available-jobs/job/144835/phd-research-fellowship-in-structural-biology-and-chromatin
[ccp4bb] Postdoc and PhD fellow at University of Oslo
The Group for Structural Biology and Chromatin (*Sekulic group)* investigates chromatin biology and epigenetics with biochemical and structural approaches. The project aims at understanding changes in chromatin during mitosis and kinetochore formation. We use various biochemical and biophysical approaches (X-ray crystallography, SAXS, SANS, hydrogen-deuterium exchange ...). In addition, we collaborate with cell biology groups to link structure with function. We seek highly motivated individuals with an excellent track record and experience in protein purification and biophysical and biochemical characterization of proteins and protein complexes. Knowledge of chromatin biology, structural biology and mass spec techniques is an advantage. We offer stimulating environment with excellent working and social benefits. *PhD fellow * A three-year program can be started ASAP. The fellowship requires admission to the PhD program at the Faculty of Mathematics and Natural Sciences. For more information see: http://www.mn.uio.no/english/research/phd/ http://www.mn.uio.no/english/research/phd/application/application.html *Postdoctoral fellow * A three-year position is available from August 2017 but earlier starting date can also be accommodated. Applications for both positions will be considered only through on-line system, so please use links below to apply. http://uio.easycruit.com/vacancy/1789469/70932?iso=gb http://uio.easycruit.com/vacancy/1789507/70932?iso=gb Nikolina Sekulic, PhD Group leader, Structural Biology and Chromatin Norwegian Center for Molecular Medicine (NCMM) Associate Professor, Department of Chemistry, University of Oslo Gaustadalléen 21 Forskningsparken 0349 OSLO Office: +47 22840511
Re: [ccp4bb] Examples of multiple ASU copies with different conformations
Another interesting example might be a structure of Mad2, protein essential in the process of chromosome segregation. Protein has 2 different secondary structure topologies and both of them are part of the AU in 2V64. There are other X-ray structures where only one of the conformations is captured in the crystal. Good luck, Nikolina Nikolina Sekulic, PhD Research Associate Department of Biochemistry and Biophysics University of Pennsylvania Stellar-Chance Laboratory 912 422 Curie Blvd, Philadelphia cell: 312-437-9095 lab: 215-898-4476 e-mail: seku...@mail.med.upenn.edu On Tue, Jan 28, 2014 at 8:26 AM, tamir gonen tgonen...@gmail.com wrote: Hi Shane, One example that comes to mind is aquaporin-z. Two protomers were found in the ASU, one contained the water channel in an open conformation while the other in a closed conformation. The structural differences are not large but the functional implication is. Here is the primary citation Architecture and selectivity in aquaporins: 2.5 a X-ray structure of aquaporin Z. http://www.ncbi.nlm.nih.gov/pubmed/14691544 *Savage* DF, Egea PF, Robles-Colmenares Y, O'Connell JD 3rd, *Stroud* RM. PLoS Biol. *2003* Dec;1(3):E72. Epub *2003* Dec 22. Kind regards Tamir gon...@hhmi.org On Jan 27, 2014, at 1:08 PM, Shane Caldwell shane.caldwel...@gmail.com wrote: Hi ccp4bb, I'm putting together a talk for some peers that highlights strengths and weaknesses of structural models for the outsider. For one point, I'd like to find some examples of proteins that show very different conformations between different copies in the ASU. One example I know of is c-Abl (1OPL), which crystallizes with both autoinhibited and active forms in the ASU, with dramatically different domain organization. I'd like to find some additional examples - can anyone suggest some other structures that have multiple copies with large structural variations? Thanks in advance! Shane Caldwell McGill University
Re: [ccp4bb] Angle between monomer of a dimer
Dear Appu, I had similar issue once. You can use Coot to overlay two structures and get the rotation matrix a1 a2 a3 b1 b2 b3 c1 c2 c3 from here you can calculate cosine of an angle using formula (a1+b2+c3-1)/2 Good luck, Nikolina Sekulic, PhD postdoctoral fellow University of Pennsylvania On Thu, Aug 23, 2012 at 2:30 PM, Appu kumar appu.kum...@gmail.com wrote: Dear all ccp4 users, I have solved the structure of a protein which is a dimer. On comparison with the other structure from PDB data bank, i found that structure solved by us show a tiltness in monomer-monomer arrangement of a dimer. May you please help me in findng the angle between two monomer. Do i need to calculate the centre of mass of the protein for calcualting the angle between them?. Thanks in advance please help me. Thank you Appu
