[ccp4bb] select from a pdb file a sphere of atoms
Dear All, I am looking for an accessible programme that allows selection of atoms from a PDB file within a sphere of inputted radius from a central atom. Thanks for any help, Stephen Cusack -- ** Dr. Stephen Cusack, FRS Head of Grenoble Outstation of the European Molecular Biology Laboratory (EMBL) Group leader in structural biology of protein-RNA complexes and viral proteins ** Email: cus...@embl.fr Website: http://www.embl.fr Tel:(33) 4 76 20 7238Secretary (33) 4 76 20 7123 Fax:(33) 4 76 20 7199 Postal address: EMBL Grenoble Outstation, 71 Avenue des Martyrs, CS 90181, 38042 Grenoble Cedex 9, France Delivery address: EMBL Grenoble Outstation, Polygone Scientifique, 71 Avenue des Martyrs, 38000 Grenoble, France ** To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB=1
[ccp4bb] Postdoctoral position in structural biology of influenza virus transcription Cusack group, EMBL Grenoble
Dear All, We are looking for a highly motivated and ambitious individual to progress our understanding of the mechanism of transcription by influenza virus RNA-dependent RNA polymerase. The work is in the frame of the ANR-funded FluTranscript project that brings together the complementary expertise of the groups of Stephen Cusack (EMBL Grenoble, project coordinator) and Nadia Naffakh (Institut Pasteur) in structural biology and molecular virology, respectively. The project follows on from a recent joint publication (Structural basis of an essential interaction between influenza polymerase and Pol II CTD. Lukarska et al, Nature 2017) and aims to reconstitute and determine the structure of functional transcription complexes. A secondary goal is to use the acquired structural information to develop new approaches to anti-influenza drug development. The Cusack group uses X-ray crystallography and cryo electron-microscopy to study the structural biology of protein-RNA complexes involved in RNA virus replication, innate immunity and cellular RNA metabolism. Group website: https://www.embl.fr/research/unit/cusack/index.html The successful candidate should have a PhD in Molecular Biology or related field and a strong background and interest in biochemistry and structural biology. We are looking for a creative and ambitious person with good communication skills and keen to work on a challenging project. Experience in insect cell expression systems, reconstitution of protein-RNA complexes, X-ray crystallography or cryo-electron microscopy would be particularly beneficial. EMBL is one of the world’s leading research organizations in the life sciences. The Headquarters Laboratory is located in Heidelberg, with additional sites in Barcelona, Grenoble, Hamburg, Hinxton, and Rome. EMBL Grenoble has excellent access to state-of-the-art structural biology technologies with the in house EM facility being equipped with a T12 microscope and 200kV Glacios/Falcon III . Additionally, we have access to the Titan Krios at the adjacent ESRF as well as those in EMBL Heidelberg. Other facilities on the Grenoble Campus include the ESRF synchrotron X-ray beamlines, neutron scattering at the ILL, high-field NMR at the IBS as well as high-throughput crystallization platform, mammalian and insect cell facilities, biophysical platform and confocal microscopy. You can find out more about this vacancy at: https://www.embl.fr/jobs/searchjobs/index.php?ref=GR00133 or by contacting Stephen Cusack: cus...@embl.fr Please apply online through www.embl.org/ Stephen Cusack -- ** Dr. Stephen Cusack, FRS Head of Grenoble Outstation of the European Molecular Biology Laboratory (EMBL) Group leader in structural biology of protein-RNA complexes and viral proteins ** Email: cus...@embl.fr Website: http://www.embl.fr Tel:(33) 4 76 20 7238Secretary (33) 4 76 20 7123 Fax:(33) 4 76 20 7199 Postal address: EMBL Grenoble Outstation, 71 Avenue des Martyrs, CS 90181, 38042 Grenoble Cedex 9, France Delivery address: EMBL Grenoble Outstation, Polygone Scientifique, 71 Avenue des Martyrs, 38000 Grenoble, France ** To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB=1
[ccp4bb] Deadline 20th October: Open Group Leader position at Grenoble Outstation of EMBL
Deadline of 20th October is approaching ! Dear All, The Grenoble Outstation of the European Molecular Biology Laboratory is seeking to recruit a Group Leader in Structural Biology of Complexes. The appointed Group Leader will be an ambitious structural biologist with an original multidisciplinary research programme oriented towards structure-function relationships of macromolecular complexes in eukaryotic systems. Particular, but not exclusive, areas of interest are host-pathogen interactions, RNA biology or computational biology/modelling. He/she will benefit from the world-class environment of the EMBL Grenoble Outstation within the Partnership for Structural Biology (www.psb-grenoble.eu) which gives access to integrated state-of-the-art structural biology technologies, including ESRF synchrotron X-ray beamlines for MX and SAXS, cryo-EM/tomography and NMR as well as protein expression screening, insect cell, biophysics, confocal microscopy and high-throughput crystallization platforms. Applicants should have a Ph.D. and at least 3 years post-doctoral experience and a strong record of achievement in structural, molecular or cell biology. Further information about research at EMBL Grenoble can be found on http://www.embl.fr/index.php Further information about the position can be found on http://www.embl.fr/aboutus/jobs/searchjobs/index.php?loc=4list=1 Stephen Cusack
[ccp4bb] Open Group Leader position at Grenoble Outstation of EMBL
Dear All, The Grenoble Outstation of the European Molecular Biology Laboratory is seeking to recruit a Group Leader in Structural Biology of Complexes. The appointed Group Leader will be an ambitious structural biologist with an original multidisciplinary research programme oriented towards structure-function relationships of macromolecular complexes in eukaryotic systems. Particular, but not exclusive, areas of interest are host-pathogen interactions, RNA biology or computational biology/modelling. He/she will benefit from the world-class environment of the EMBL Grenoble Outstation within the Partnership for Structural Biology (www.psb-grenoble.eu) which gives access to integrated state-of-the-art structural biology technologies, including ESRF synchrotron X-ray beamlines for MX and SAXS, cryo-EM/tomography and NMR as well as protein expression screening, insect cell, biophysics, confocal microscopy and high-throughput crystallization platforms. Applicants should have a Ph.D. and at least 3 years post-doctoral experience and a strong record of achievement in structural, molecular or cell biology. Further information about research at EMBL Grenoble can be found on http://www.embl.fr/index.php Further information about the position can be found on http://www.embl.fr/aboutus/jobs/searchjobs/index.php?loc=4list=1 Stephen Cusack --
[ccp4bb] Open Group Leader position at Grenoble Outstation of EMBL
Dear All, The Grenoble Outstation of the European Molecular Biology Laboratory is seeking to recruit a Group Leader in Structural Biology of Complexes. The appointed Group Leader will be an ambitious structural biologist with an original multidisciplinary research programme oriented towards structure-function relationships of macromolecular complexes in eukaryotic systems. Particular, but not exclusive, areas of interest are host-pathogen interactions, RNA biology or computational biology/modelling. He/she will benefit from the world-class environment of the EMBL Grenoble Outstation within the Partnership for Structural Biology (www.psb-grenoble.eu) which gives access to integrated state-of-the-art structural biology technologies, including ESRF synchrotron X-ray beamlines for MX and SAXS, cryo-EM/tomography and NMR as well as protein expression screening, insect cell, biophysics, confocal microscopy and high-throughput crystallization platforms. Applicants should have a Ph.D. and at least 3 years post-doctoral experience and a strong record of achievement in structural, molecular or cell biology. Further information about research at EMBL Grenoble can be found on http://www.embl.fr/index.php Further information about the position can be found on http://www.embl.fr/aboutus/jobs/searchjobs/index.php?loc=4list=1 Stephen Cusack -- ** Dr. Stephen Cusack, Head of Grenoble Outstation of EMBL Group leader in structural biology of protein-RNA complexes and viral proteins Joint appointment in EMBL Genome Biology Programme Director of CNRS-UJF-EMBL International Unit (UMI 3265) for Virus Host Cell Interactions (UVHCI) ** Email: cus...@embl.fr Website: http://www.embl.fr Tel:(33) 4 76 20 7238Secretary (33) 4 76 20 7123 Fax:(33) 4 76 20 7199 Postal address: EMBL Grenoble Outstation, 6 Rue Jules Horowitz, BP181, 38042 Grenoble Cedex 9, France Delivery address: EMBL Grenoble Outstation, Polygone Scientifique, 6 Rue Jules Horowitz, 38042 Grenoble, France **
[ccp4bb] PDB deposition of internally truncated protein
Dear All, I need to deposit in the PDB the co-ordinates of a protein with an internal truncation. If I do this in the normal way with consecutive numbering according to the actual polypeptide sequence in the crystal, the residue numbers after the truncation will not correspond to the equivalent residues in the untruncated protein (of which there is also a structure). Is there any good solution to this problem? thanks Stephen Cusack -- ** Dr. Stephen Cusack, Head of Grenoble Outstation of EMBL Group leader in structural biology of protein-RNA complexes and viral proteins Joint appointment in EMBL Genome Biology Programme Director of CNRS-UJF-EMBL International Unit (UMI 3265) for Virus Host Cell Interactions (UVHCI) ** Email: cus...@embl.fr Website: http://www.embl.fr Tel:(33) 4 76 20 7238Secretary (33) 4 76 20 7123 Fax:(33) 4 76 20 7199 Postal address: EMBL Grenoble Outstation, 6 Rue Jules Horowitz, BP181, 38042 Grenoble Cedex 9, France Delivery address: EMBL Grenoble Outstation, Polygone Scientifique, 6 Rue Jules Horowitz, 38042 Grenoble, France **
[ccp4bb] Deadline Nov. 6th: Post-doctoral position on RIG-I like helicases in Cusack group at EMBL Grenoble
A Postdoctoral Fellowship in the Structural Biology of RIG-I like helicases is available in the group of Dr. Stephen Cusack at the EMBL Grenoble Outstation. For more details and applications see http://ig14.i-grasp.com//fe/tpl_embl01.asp?newms=jjid=46569aid=15470 The group of Stephen Cusack at the EMBL Grenoble Outstation is seeking to recruit a motivated post-doctoral fellow to work on the structural biology of RIG-I like helicases. These cytoplasmic signalling proteins are pattern recognition receptors of the innate immune system and are critical for the early detection of viral infections through recognition of non-self viral RNA molecular patterns, which triggers interferon production. The Cusack group has recently determined crystal structures of the auto-repressed and RNA/ATP activated states of RIG-I leading to the first structure-based model of the RIG-I activation mechanism. This work has just been published in Cell (http://www.cell.com/abstract/S0092-8674%2811%2901150-0). Future work will refine this model and extend structural work to the two other RIG-I like helicases, MDA5 and LGP2 and the downstream signalling partner MAVS. The EMBL Grenoble Outstation is embedded in the Grenoble Partnership for Structural Biology (PSB), which is a uniquely rich environment for structural biology providing access to state-of-the-art facilities for protein production, biophysical characterisation, crystallisation and structure determination (ESRF synchrotron, NMR, EM). Candidates should have a PhD and a record of successfully working on challenging structural biology problems. Experience in some of the following will be an advantage: protein expression in insect cells, in vitro production of RNA and reconstitution of protein-RNA complexes, synchrotron based protein crystallography, functional assays in cells. Candidates interested in biophysical mechanistic studies of RLHs in vitro or in living cells (e.g. by fluorescence techniques) are also encouraged to apply. Funding is available for an initial two year period with the possibility of staying up to five years. -- ** Dr. Stephen Cusack, Head of Grenoble Outstation of EMBL Group leader in structural biology of protein-RNA complexes and viral proteins Joint appointment in EMBL Genome Biology Programme Director of CNRS-UJF-EMBL International Unit (UMI 3265) for Virus Host Cell Interactions (UVHCI) ** Email: cus...@embl.fr Website: http://www.embl.fr Tel:(33) 4 76 20 7238Secretary (33) 4 76 20 7123 Fax:(33) 4 76 20 7199 Postal address: EMBL Grenoble Outstation, 6 Rue Jules Horowitz, BP181, 38042 Grenoble Cedex 9, France Delivery address: EMBL Grenoble Outstation, Polygone Scientifique, 6 Rue Jules Horowitz, 38042 Grenoble, France **
[ccp4bb] Post-doctoral position on RIG-I like helicases at EMBL Grenoble
A Postdoctoral Fellowship in the Structural Biology of RIG-I like helicases is available in the group of Dr. Stephen Cusack at the EMBL Grenoble Outstation. For more details and applications see http://ig14.i-grasp.com//fe/tpl_embl01.asp?newms=jjid=46569aid=15470 The group of Stephen Cusack at the EMBL Grenoble Outstation is seeking to recruit a motivated post-doctoral fellow to work on the structural biology of RIG-I like helicases. These cytoplasmic signalling proteins are pattern recognition receptors of the innate immune system and are critical for the early detection of viral infections through recognition of non-self viral RNA molecular patterns, which triggers interferon production. The Cusack group has recently determined crystal structures of the auto-repressed and RNA/ATP activated states of RIG-I leading to the first structure-based model of the RIG-I activation mechanism. This work is in press in Cell. Future work will refine this model and extend structural work to the two other RIG-I like helicases, MDA5 and LGP2 and the downstream signalling partner MAVS. The EMBL Grenoble Outstation is embedded in the Grenoble Partnership for Structural Biology (PSB), which is a uniquely rich environment for structural biology providing access to state-of-the-art facilities for protein production, biophysical characterisation, crystallisation and structure determination (ESRF synchrotron, NMR, EM). Candidates should have a PhD and a record of successfully working on challenging structural biology problems. Experience in some of the following will be an advantage: protein expression in insect cells, in vitro production of RNA and reconstitution of protein-RNA complexes, synchrotron based protein crystallography, functional assays in cells. Candidates interested in biophysical mechanistic studies of RLHs in vitro or in living cells (e.g. by fluorescence techniques) are also encouraged to apply. Funding is available for an initial two year period with the possibility of staying up to five years. -- ** Dr. Stephen Cusack, Head of Grenoble Outstation of EMBL Group leader in structural biology of protein-RNA complexes and viral proteins Joint appointment in EMBL Genome Biology Programme Director of CNRS-UJF-EMBL International Unit (UMI 3265) for Virus Host Cell Interactions (UVHCI) ** Email: cus...@embl.fr Website: http://www.embl.fr Tel:(33) 4 76 20 7238Secretary (33) 4 76 20 7123 Fax:(33) 4 76 20 7199 Postal address: EMBL Grenoble Outstation, 6 Rue Jules Horowitz, BP181, 38042 Grenoble Cedex 9, France Delivery address: EMBL Grenoble Outstation, Polygone Scientifique, 6 Rue Jules Horowitz, 38042 Grenoble, France **
[ccp4bb] identifying hinges in flexible proteins
We have structures of two states of a large multi-domain protein in which domain movements of up to 30 degrees are observed. Is there a programme available which attempts to determine rigid-body units and hinge regions in an 'unbiased' way? thanks for your suggestions, Stephen Cusack -- ** Dr. Stephen Cusack, Head of Grenoble Outstation of EMBL Group leader in structural biology of protein-RNA complexes and viral proteins Joint appointment in EMBL Genome Biology Programme Director of CNRS-UJF-EMBL International Unit (UMI 3265) for Virus Host Cell Interactions (UVHCI) ** Email: cus...@embl.fr Website: http://www.embl.fr Tel:(33) 4 76 20 7238Secretary (33) 4 76 20 7123 Fax:(33) 4 76 20 7199 Postal address: EMBL Grenoble Outstation, 6 Rue Jules Horowitz, BP181, 38042 Grenoble Cedex 9, France Delivery address: EMBL Grenoble Outstation, Polygone Scientifique, 6 Rue Jules Horowitz, 38042 Grenoble, France **
[ccp4bb] Head of the Crystallisation Facility at EMBL Grenoble
Vacancy for Head of the Crystallisation Facility at EMBL Grenoble The EMBL Grenoble Outstation runs the very successful high throughput crystallisation service of the Partnership for Structural Biology in Grenoble (PSB, http://www.psb-grenoble.eu/) http://www.psb-grenoble.eu/spip.php?rubrique16. It is one of the largest such facilities in Europe and serves over 200 PSB scientists as well as external users via the EU funded P-CUBE programme (http://www.p-cube.eu/ http://www.p-cube.eu/). We wish to recruit a dynamic scientist to take responsibility for the platform and oversee its future operations and development. The successful candidate will be expected to maintain a high-quality, state-of-the-art user service as well as develop and upgrade the facility by implementation of innovative crystallisation technologies. In particular the platform should be closely integrated with both upstream protein production facilities and downstream X-ray data collection facilities, in collaboration with other teams at the Outstation. The deadline for applications is 29th May 2011. For further information and applications see: http://www.embl.fr/aboutus/jobs/searchjobs/index.php?newlang=1newms=srsearchregion=670 Informal inquiries can be made to Stephen Cusack, cus...@embl.fr -- ** Dr. Stephen Cusack, Head of Grenoble Outstation of EMBL Group leader in structural biology of protein-RNA complexes and viral proteins Joint appointment in EMBL Genome Biology Programme Director of CNRS-UJF-EMBL International Unit (UMI 3265) for Virus Host Cell Interactions (UVHCI) ** Email: cus...@embl.fr Website:http://www.embl.fr Tel:(33) 4 76 20 7238Secretary (33) 4 76 20 7123 Fax:(33) 4 76 20 7199 Postal address: EMBL Grenoble Outstation, 6 Rue Jules Horowitz, BP181, 38042 Grenoble Cedex 9, France Delivery address: EMBL Grenoble Outstation, Polygone Scientifique, 6 Rue Jules Horowitz, 38042 Grenoble, France **
Re: [ccp4bb] FW: Refmac 5.5 vs 5.2; repost with data
Dear Sean, I have noticed this and more to the point the geometry as assessed by MOLPROBITY is much worse for the latest version of REFMAC (3.0068). I have tested a few other versions of REFMAC and the results vary quite a lot. For exactly the same run, I get the best results with an old version Refmac_5.2.0019! For my structure, exactly the same run gives the R-factors 0.218, 0.268 with 5.2.0019 (compare 0.2326 R-free 0.2915 for latest Refmac version 3.0068) and the also the best MOLPROBITY scores for (clashscore 9.03 for , compared with 22.0 for 3.0068). And the map is actually noticeably better after refining with Refmac_5.2.0019. I have been in touch with Garib about this and he is looking into it. Stephen Hi all, I have just installed the new CCP4I package for Windows, and refined a structure I was working on using an older version (CCP4I 6.0.2), and the refinement statistics are significantly worse when using the same input files. Under Refmac 5.2 the details were: $$ Ncyc Rfact Rfree FOM LLG rmsBOND rmsANGLE rmsCHIRAL $$ $$ 0 0.150 0.1890.886 481182.90.0111.2080.147 1 0.147 0.1880.887 480211.90.0101.1700.147 2 0.147 0.1880.887 480049.60.0101.1520.147 3 0.147 0.1880.887 479927.90.0101.1470.147 4 0.147 0.1880.887 479847.20.0101.1440.147 5 0.147 0.1880.887 479805.50.0101.1430.148 $$ Where Refmac 5.5 gives: $$ NcycRfactRfree FOM -LL -LLfree rmsBOND zBOND rmsANGL zANGL rmsCHIRAL $$ $$ 0 0.1738 0.2163 0.874465814. 25317.2 0.0106 0.439 1.208 0.519 0.147 1 0.1723 0.2158 0.875465007. 25296.4 0.0102 0.410 1.178 0.510 0.147 2 0.1716 0.2155 0.875464584. 25285.4 0.0102 0.403 1.158 0.506 0.147 3 0.1711 0.2155 0.875464297. 25278.2 0.0101 0.401 1.145 0.503 0.146 4 0.1708 0.2155 0.875464090. 25271.8 0.0101 0.399 1.137 0.501 0.146 5 0.1706 0.2155 0.876463961. 25269.1 0.0101 0.398 1.131 0.500 0.146 $$ I have been through the header of both files to check that all of the settings are the same. One thing I did notice is that the Estimated number of reflections is now 111528, where Refmac 5.2 had this value at 101968. I don't know whether this is relevant to the problem I'm seeing, but it does seem suspicious. The new Refmac has also apparently read one more reflection than the old (84317 vs 84316). Has anyone else seen this problem, and is there anything I can do to fix it (I was liking my old statistics!)? Thanks in advance, Sean -- ** Dr. Stephen Cusack, Head of Grenoble Outstation of EMBL Group leader in structural biology of protein-RNA complexes and viral proteins Joint appointment in EMBL Gene Expression Programme Director of CNRS-UJF-EMBL Mixed Unit (UMR 5233) for Virus Host Cell Interactions (UVHCI) ** Email: cus...@embl.fr Website: http://www.embl.fr Tel:(33) 4 76 20 7238Secretary (33) 4 76 20 7306 Fax:(33) 4 76 20 7786 or (33) 4 76 20 7199 Postal address: EMBL Grenoble Outstation, 6 Rue Jules Horowitz, BP181, 38042 Grenoble Cedex 9, France Delivery address: EMBL Grenoble Outstation, Polygone Scientifique, 6 Rue Jules Horowitz, 38042 Grenoble, France **
[ccp4bb] EMBL Grenoble Staff Scientist : New SAXS Beamline on ID14-EH3 at the ESRF
EMBL Grenoble Staff Scientist : New SAXS Beamline on ID14-EH3 at the ESRF Job description: A scientist is required to join a team within the Partnership for Structural Biology (EMBL, ESRF, ILL and IBS, http://psb.esrf.fr/) which is establishing a unique and integrated SAXS-SANS platform, making use of the intense synchrotron X-ray and neutron beams of respectively the ERSF and ILL. The successful candidate will be responsible for construction, commissioning and user support of a new state-of-the-art SAXS beamline, dedicated to biology, to be built on undulator beamline ID14-EH3 at the ESRF. The beamline will cater to the growing needs of the European structural biology community who wish to combine SAXS studies with crystallography and other techniques. He/She will benefit from strong technical support from the ESRF MX and Soft Condensed Matter groups and other ESRF services as well as the EMBL Grenoble diffraction instrumentation group. In addition there will be a strong collaboration with the SAXS scientists from the EMBL Hamburg Outstation both to transfer expertise and to provide test facilities during the conception and construction phase of the future SAXS beamline at Petra III in Hamburg. Beamline construction is expected to proceed rapidly from the beginning of 2008 and be completed within nine months. *Qualifications and experience:* Applicants for this position are expected to hold a PhD in a related specialization, as well as have postdoctoral experience in instrumentation and software for small-angle scattering in physics, chemistry or biology, preferably at a synchrotron. At least some knowledge of, and demonstrable interest in, biology is required. Commencing date: As soon as possible following the closing date Contract: An initial contract of 3 years will be offered to the successful candidate. This can be renewed, depending on circumstances at the time of review. *Closing date*: 2 December 2007 *Outstation web page*: http://www.embl-grenoble.fr/ *Job advert web page*: http://www-db.embl.de/jss/servlet/de.embl.bk.emblGroups.JobsPage/07069.html To apply, please email a curriculum vitae, three references and a concise description of research interests and future plans quoting ref. no. W/07/069 in the subject line, to: [EMAIL PROTECTED] Informal enquiries to me Stephen Cusack -- ** Dr. Stephen Cusack, Head of Grenoble Outstation of EMBL Group leader in structural biology of protein-RNA complexes and viral proteins Joint appointment in EMBL Gene Expression Programme Director of CNRS-UJF-EMBL Mixed Unit (UMR 5233) for Virus Host Cell Interactions (UVHCI) ** Email: [EMAIL PROTECTED] Website: http://www.embl.fr Tel:(33) 4 76 20 7238Secretary (33) 4 76 20 7306 Fax:(33) 4 76 20 7786 or (33) 4 76 20 7199 Postal address: EMBL Grenoble Outstation, 6 Rue Jules Horowitz, BP181, 38042 Grenoble Cedex 9, France Delivery address: EMBL Grenoble Outstation, Polygone Scientifique, 6 Rue Jules Horowitz, 38042 Grenoble, France **
[ccp4bb] surface plots
Dear colleagues, I believe there is a server somewhere that takes 3D co-ordinates and displays the protein surface coloured according to sequence conservation with homologous proteins. Please can you remind the name of the website? thanks Stephen Cusack -- ** Dr. Stephen Cusack, Head of Grenoble Outstation of EMBL Group leader in structural biology of protein-RNA complexes and viral proteins Joint appointment in EMBL Gene Expression Programme Director of CNRS-UJF-EMBL Mixed Unit (UMR 5233) for Virus Host Cell Interactions (UVHCI) ** Email: [EMAIL PROTECTED] Website: http://www.embl.fr Tel:(33) 4 76 20 7238Secretary (33) 4 76 20 7306 Fax:(33) 4 76 20 7786 or (33) 4 76 20 7199 Postal address: EMBL Grenoble Outstation, 6 Rue Jules Horowitz, BP181, 38042 Grenoble Cedex 9, France Delivery address: EMBL Grenoble Outstation, Polygone Scientifique, 6 Rue Jules Horowitz, 38042 Grenoble, France **
[ccp4bb] EMBL Grenoble Team Leader: Synchrotron Methods for Structural Biology
*EMBL Grenoble Team Leader: Synchrotron Methods for Structural Biology http://www-db.embl.de/jss/servlet/de.embl.bk.emblGroups.JobsPage/07065.html?EmblGR=x Job Description*: We are looking for a structural biologist who has a focus on advanced crystallographic methods and instrumentation, particularly with relation to synchrotron radiation. The appointed Team Leader will manage, together with the Head of Outstation, the long-term collaboration between the EMBL Grenoble Outstation and the European Synchrotron Radiation Facility (ESRF) concerning the development and operation of the automated protein crystallography beamlines. He/she will oversee the activities of the three existing EMBL beamline scientists currently responsible for the dedicated microfocus beamline ID23-2, the MAD beamline ID14-EH4 and the CRG beamline BM14 (co-operated with MRC-France) as well as a SAXS beamline scientist currently under recruitment. He/she will collaborate in particular with the Outstation Diffraction Instrumentation team led by Florent Cipriani, which has developed state-of-the-art sample handling equipment currently installed on all ESRF MX beamlines (microdiffractometer, sample changer, minikappa) and with the ESRF MX group led by Sean McSweeney. He/she will develop a research programme focused on advanced crystallographic methods and instrumentation coupled to structural biological problems or more generally on new synchrotron techniques that might have an impact in biology and which take advantage of the unique environment at the world-leading 3rd generation synchrotron site in Grenoble. *Commencing date:* As soon as possible following the closing date* *Contract:* An initial contract of 3 years will be offered to the successful candidate. This can be renewed, depending on circumstances at the time of review.* Closing date:* 8 July 2007 * Web page:* http://www.embl-grenoble.fr/ *To apply, please email a curriculum vitae, three references and a concise description of research interests and future plans quoting ref. no. W/07/065 in the subject line, to: [EMAIL PROTECTED] *Informal enquiries to me* *Stephen Cusack* ** Dr. Stephen Cusack, Head of Grenoble Outstation of EMBL Group leader in structural biology of protein-RNA complexes and viral proteins Joint appointment in EMBL Gene Expression Programme ** Email: [EMAIL PROTECTED] Website: http://www.embl-grenoble.fr Tel: (33) 4 76 20 7238 Secretary (33) 4 76 20 7306 Fax: (33) 4 76 20 7786 or (33) 4 76 20 7199 Postal address: EMBL c/o ILL, BP181, 38042 Grenoble Cedex 9, France Delivery address: EMBL c/o ILL, Polygone Scientifique, 6 Rue Jules Horowitz, 38042 Grenoble, France ** -- ** Dr. Stephen Cusack, Head of Grenoble Outstation of EMBL Group leader in structural biology of protein-RNA complexes and viral proteins Joint appointment in EMBL Gene Expression Programme Director of CNRS-UJF-EMBL Mixed Unit (UMR 5233) for Virus Host Cell Interactions (UVHCI) ** Email: [EMAIL PROTECTED] Website: http://www.embl.fr Tel:(33) 4 76 20 7238Secretary (33) 4 76 20 7306 Fax:(33) 4 76 20 7786 or (33) 4 76 20 7199 Postal address: EMBL, 6 Rue Jules Horowitz, BP181, 38042 Grenoble Cedex 9, France Delivery address: EMBL c/o ILL, Polygone Scientifique, 6 Rue Jules Horowitz, 38042 Grenoble, France **
[ccp4bb] EMBL Grenoble Staff Scientist : New SAXS Beamline on ID14-EH3 at the ESRF
*EMBL Grenoble Staff Scientist : New SAXS Beamline on ID14-EH3 at the ESRF* http://www-db.embl.de/jss/servlet/de.embl.bk.emblGroups.JobsPage/07069.html?EmblGR=x *Job description:* A scientist is required to join a team within the Partnership for Structural Biology (EMBL, ESRF, ILL and IBS, http://psb.esrf.fr/) which is establishing a unique and integrated SAXS-SANS platform, making use of the intense synchrotron X-ray and neutron beams of respectively the ERSF and ILL. The successful candidate will be responsible for construction, commissioning and user support of a new state-of-the-art SAXS beamline, dedicated to biology, to be built on undulator beamline ID14-EH3 at the ESRF. The beamline will cater to the growing needs of the European structural biology community who wish to combine SAXS studies with crystallography and other techniques. He/She will benefit from strong technical support from the ESRF MX and Soft Condensed Matter groups and other ESRF services as well as the EMBL Grenoble diffraction instrumentation group. In addition there will be a strong collaboration with the SAXS scientists from the EMBL Hamburg Outstation both to transfer expertise and to provide test facilities during the conception and construction phase of the future SAXS beamline at Petra III in Hamburg. *Qualifications and experience:* Applicants for this position are expected to hold a PhD in a related specialization, as well as have postdoctoral experience in instrumentation and software for small-angle scattering in physics, chemistry or biology, preferably at a synchrotron. At least some knowledge of, and demonstrable interest in, biology is required. *Commencing date:* As soon as possible following the closing date *Contract:* An initial contract of 3 years will be offered to the successful candidate. This can be renewed, depending on circumstances at the time of review. *Closing date*: 8 July 2007 *Web page*: http://www.embl-grenoble.fr/ To apply, please email a curriculum vitae, three references and a concise description of research interests and future plans quoting ref. no. W/07/065 in the subject line, to: [EMAIL PROTECTED] Informal enquiries to me Stephen Cusack * * http://www.embl-grenoble.fr/ ** Dr. Stephen Cusack, Head of Grenoble Outstation of EMBL Group leader in structural biology of protein-RNA complexes and viral proteins Joint appointment in EMBL Gene Expression Programme ** Email: [EMAIL PROTECTED] Website: http://www.embl-grenoble.fr Tel:(33) 4 76 20 7238Secretary (33) 4 76 20 7306 Fax:(33) 4 76 20 7786 or (33) 4 76 20 7199 Postal address: EMBL c/o ILL, BP181, 38042 Grenoble Cedex 9, France Delivery address: EMBL c/o ILL, Polygone Scientifique, 6 Rue Jules Horowitz, 38042 Grenoble, France ** -- ** Dr. Stephen Cusack, Head of Grenoble Outstation of EMBL Group leader in structural biology of protein-RNA complexes and viral proteins Joint appointment in EMBL Gene Expression Programme Director of CNRS-UJF-EMBL Mixed Unit (UMR 5233) for Virus Host Cell Interactions (UVHCI) ** Email: [EMAIL PROTECTED] Website: http://www.embl.fr Tel:(33) 4 76 20 7238Secretary (33) 4 76 20 7306 Fax:(33) 4 76 20 7786 or (33) 4 76 20 7199 Postal address: EMBL, 6 Rue Jules Horowitz, BP181, 38042 Grenoble Cedex 9, France Delivery address: EMBL c/o ILL, Polygone Scientifique, 6 Rue Jules Horowitz, 38042 Grenoble, France **
[ccp4bb] humidity dependent phase transition
Dear All, We are looking for crystals to test a new device for in situ changing the humidity on the X-ray beamline. The requirements are (protein) crystals that are relatively easy to grow and that have a clear change in diffraction pattern upon change in humidity at room temperature. Any suggestions welcome, Stephen Cusack -- ** Dr. Stephen Cusack, Head of Grenoble Outstation of EMBL Group leader in structural biology of protein-RNA complexes and viral proteins Joint appointment in EMBL Gene Expression Programme Director of CNRS-UJF-EMBL Mixed Unit (UMR 5233) for Virus Host Cell Interactions (UVHCI) ** Email: [EMAIL PROTECTED] Website: http://www.embl.fr Tel:(33) 4 76 20 7238Secretary (33) 4 76 20 7306 Fax:(33) 4 76 20 7786 or (33) 4 76 20 7199 Postal address: EMBL, 6 Rue Jules Horowitz, BP181, 38042 Grenoble Cedex 9, France Delivery address: EMBL c/o ILL, Polygone Scientifique, 6 Rue Jules Horowitz, 38042 Grenoble, France **
[ccp4bb] EMBL Grenoble Team Leader: Synchrotron Methods for Structural Biology
*EMBL Grenoble Team Leader: Synchrotron Methods for Structural Biology Job Description*: We are looking for a structural biologist who has a focus on advanced crystallographic methods and instrumentation, particularly with relation to synchrotron radiation. The appointed Team Leader will manage, together with the Head of Outstation, the long-term collaboration between the EMBL Grenoble Outstation and the European Synchrotron Radiation Facility (ESRF) concerning the development and operation of the automated protein crystallography beamlines. He/she will oversee the activities of the three existing EMBL beamline scientists currently responsible for the dedicated microfocus beamline ID23-2, the MAD beamline ID14-EH4 and the CRG beamline BM14 (co-operated with MRC-France) as well as a SAXS beamline scientist currently under recruitment. He/she will collaborate in particular with the Outstation Diffraction Instrumentation team led by Florent Cipriani, which has developed state-of-the-art sample handling equipment currently installed on all ESRF MX beamlines (microdiffractometer, sample changer, minikappa) and with the ESRF MX group led by Sean McSweeney. He/she will develop a research programme focused on advanced crystallographic methods and instrumentation coupled to structural biological problems or more generally on new synchrotron techniques that might have an impact in biology and which take advantage of the unique environment at the world-leading 3rd generation synchrotron site in Grenoble. *Commencing date:* As soon as possible following the closing date* Contract:* An initial contract of 3 years will be offered to the successful candidate. This can be renewed, depending on circumstances at the time of review.* Closing date:* 8 July 2007 * Web page:* http://www.embl-grenoble.fr/ *To apply, please email a curriculum vitae, three references and a* *concise description of research interests and future plans quoting ref.* *no. W/07/065 in the subject line, to: [EMAIL PROTECTED] mailto:[EMAIL PROTECTED]* *Informal enquiries to me* *Stephen Cusack* ** Dr. Stephen Cusack, Head of Grenoble Outstation of EMBL Group leader in structural biology of protein-RNA complexes and viral proteins Joint appointment in EMBL Gene Expression Programme ** Email: [EMAIL PROTECTED] Website: http://www.embl-grenoble.fr Tel: (33) 4 76 20 7238 Secretary (33) 4 76 20 7306 Fax: (33) 4 76 20 7786 or (33) 4 76 20 7199 Postal address: EMBL c/o ILL, BP181, 38042 Grenoble Cedex 9, France Delivery address: EMBL c/o ILL, Polygone Scientifique, 6 Rue Jules Horowitz, 38042 Grenoble, France **
[ccp4bb] EMBL Grenoble Staff Scientist : New SAXS Beamline on ID14-EH3 at the ESRF
*EMBL Grenoble Staff Scientist : New SAXS Beamline on ID14-EH3 at the ESRF Job description:* A scientist is required to join a team within the Partnership for Structural Biology (EMBL, ESRF, ILL and IBS, http://psb.esrf.fr/) which is establishing a unique and integrated SAXS-SANS platform, making use of the intense synchrotron X-ray and neutron beams of respectively the ERSF and ILL. The successful candidate will be responsible for construction, commissioning and user support of a new state-of-the-art SAXS beamline, dedicated to biology, to be built on undulator beamline ID14-EH3 at the ESRF. The beamline will cater to the growing needs of the European structural biology community who wish to combine SAXS studies with crystallography and other techniques. He/She will benefit from strong technical support from the ESRF MX and Soft Condensed Matter groups and other ESRF services as well as the EMBL Grenoble diffraction instrumentation group. In addition there will be a strong collaboration with the SAXS scientists from the EMBL Hamburg Outstation both to transfer expertise and to provide test facilities during the conception and construction phase of the future SAXS beamline at Petra III in Hamburg. * Qualifications and experience:* Applicants for this position are expected to hold a PhD in a related specialization, as well as have postdoctoral experience in instrumentation and software for small-angle scattering in physics, chemistry or biology, preferably at a synchrotron. At least some knowledge of, and demonstrable interest in, biology is required. Commencing date: As soon as possible following the closing date *Contract:* An initial contract of 3 years will be offered to the successful candidate. This can be renewed, depending on circumstances at the time of review. *Closing date*: 8 July 2007 *Web page*: http://www.embl-grenoble.fr/ To apply, please email a curriculum vitae, three references and a concise description of research interests and future plans quoting ref. no. W/07/065 in the subject line, to: [EMAIL PROTECTED] Informal enquiries to me Stephen Cusack * * http://www.embl-grenoble.fr/ ** Dr. Stephen Cusack, Head of Grenoble Outstation of EMBL Group leader in structural biology of protein-RNA complexes and viral proteins Joint appointment in EMBL Gene Expression Programme ** Email: [EMAIL PROTECTED] Website: http://www.embl-grenoble.fr Tel:(33) 4 76 20 7238Secretary (33) 4 76 20 7306 Fax:(33) 4 76 20 7786 or (33) 4 76 20 7199 Postal address: EMBL c/o ILL, BP181, 38042 Grenoble Cedex 9, France Delivery address: EMBL c/o ILL, Polygone Scientifique, 6 Rue Jules Horowitz, 38042 Grenoble, France **
[ccp4bb] heavy metal labelled GTP derivatives
Does anyone know of any heavy metal labelled GTP derivatives and if so where to get them? thanks Stephen -- ** Dr. Stephen Cusack, Head of Grenoble Outstation of EMBL Group leader in structural biology of protein-RNA complexes and viral proteins Joint appointment in EMBL Gene Expression Programme ** Email: [EMAIL PROTECTED] Website: http://www.embl-grenoble.fr Tel:(33) 4 76 20 7238Secretary (33) 4 76 20 7306 Fax:(33) 4 76 20 7786 or (33) 4 76 20 7199 Postal address: EMBL c/o ILL, BP181, 38042 Grenoble Cedex 9, France Delivery address: EMBL c/o ILL, Polygone Scientifique, 6 Rue Jules Horowitz, 38042 Grenoble, France **
[ccp4bb] Post-Doctoral Fellowship: Biological Neutron Crystallography at EMBL Grenoble
*Biological Neutron Crystallography at EMBL Grenoble and the Institut Laue-Langevin* *Job description:* We seek a postdoctoral research fellow to work in the field of neutron protein crystallography using the newly upgraded neutron Laue diffractometer, LADI-III, at the Institute Laue Lamgevin (ILL). As well as developing their own research projects, the successful candidate will assist in the running of the LADI-III user program by providing support for visiting scientists in planning and performing neutron crystallography experiments and in most cases collaborating closely on the analysis and interpretation of the data. He/She will also be involved in the development and implementation of software for neutron protein crystallography. EMBL and ILL, together with the ESRF and IBS, form the Partnership for Structural Biology (PSB, http://psb.esrf.fr/index.html) a dynamic on-site collaboration between several Grenoble-based national and international structural biology institutes. The PSB runs a facility for in vivo deuteration which will give the successful candidate the opportunity to carry out his or her research in the leading environment for biological neutron diffraction in the world. The post represents an excellent opportunity for a young postdoctoral scientist to develop their expertise, broaden their experience and interact with leading scientists from around the world. *Qualifications:* The candidate must hold a PhD and have a strong interest and scientific in structural biology. Experience in either X-ray or neutron macromolecular crystallography is required and knowledge of scientific programming would be advantageous. Good communication skills and the ability to collaborate with international teams are essential. *Contract:* A contract of 2 years will be offered to the successful candidate *Closing date:* 15 April 2007 *Web page:* http://www.embl-grenoble.fr/ EMBL is an inclusive, equal opportunity employer offering attractive conditions and benefits appropriate to an international research organisation. Please note that EMBL does not return CVs or attached documents to applicants. Further information on the position from Dr Stephen Cusack([EMAIL PROTECTED] mailto:[EMAIL PROTECTED]) and Dr Matthew Blakely ([EMAIL PROTECTED] mailto:[EMAIL PROTECTED]) *To apply for this post, please send a CV, a publication list, and a list of three referees quoting ref. no. W/07/PD/07 to:[EMAIL PROTECTED] mailto:[EMAIL PROTECTED]* Personnel, EMBL, Postfach 10.2209, 69012 Heidelberg, Germany. *Fax: +49 6221 387555* *E-mail:* [EMAIL PROTECTED] mailto:[EMAIL PROTECTED] -- ** Dr. Stephen Cusack, Head of Grenoble Outstation of EMBL Group leader in structural biology of protein-RNA complexes and viral proteins Joint appointment in EMBL Gene Expression Programme ** Email: [EMAIL PROTECTED] Website: http://www.embl-grenoble.fr Tel:(33) 4 76 20 7238Secretary (33) 4 76 20 7306 Fax:(33) 4 76 20 7786 or (33) 4 76 20 7199 Postal address: EMBL c/o ILL, BP181, 38042 Grenoble Cedex 9, France Delivery address: EMBL c/o ILL, Polygone Scientifique, 6 Rue Jules Horowitz, 38042 Grenoble, France **
