Dear Catrine
can you comment on the pH range of your crystallization hits compared to the pH
of the sample you use for your crystallization trials? The pH can be a major
issue depending on the nature of the interaction interface.
best regards
Savvas
On 14 Mar 2011, at 13:21, Catrine Berthold Siöberg wrote:
Dear CCP4bb,
I am trying to solve the structure of a protein-protein complex. The two
proteins are co-expressed and co-purified and I get a homogeneous 1:3
complex, which is the predicted composition. I get several hits using
commercial crystallisation screens (including the MD ProPlex screen), but all
crystals only contain the trimeric, more stable protein of the two.
I am now considering to crosslink them, but my problem is that I cannot
modify them separately to get a specific crosslink, since one of the proteins
precipitates by itself.
I would appreciate all protocols for crosslinking of proteins aimed for
crystallisation, or references to structures solved of crosslinked proteins.
Thank You,
Catrine
--
Catrine Berthold Siöberg, PhD
Stockholm Center for Biomembrane Research
Department of Biochemistry and Biophysics
Stockholm University
S-106 91 Stockholm
Sweden
e-mail: catrine.siob...@dbb.su.se
phone: +468-162451
