Re: [ccp4bb] Lyophilized protein sample (purchased)
Dear Rex, for me it works in most cases. After all, vendors who sell inactive proteins usually get troubles with their customers. However, as always in crystallography, there is no guarantee that it will crystallize and in this case you should contact the vendor to get a frozen solution. If the protein you buy is not dirt-cheap (like trypsin), the price involved is such that they will do it for you. Best regards, Herman From: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] On Behalf Of REX PALMER Sent: Friday, May 06, 2011 10:01 PM To: CCP4BB@JISCMAIL.AC.UK Subject: [ccp4bb] Lyophilized protein sample (purchased) We have purchased a 5mg commercial sample of a protein we want to crystallize. On arrival it transpired that the sample had been lyophilized. Does anyone know if this is likely to give problems and if so what can/should be done about it? Thanks in advance Rex Palmer Birkbeck College
[ccp4bb] Lyophilized protein sample (purchased)
We have purchased a 5mg commercial sample of a protein we want to crystallize. On arrival it transpired that the sample had been lyophilized. Does anyone know if this is likely to give problems and if so what can/should be done about it? Thanks in advance Rex Palmer Birkbeck College
Re: [ccp4bb] Lyophilized protein sample (purchased)
I think that's impossible to say. Some proteins lyophilize fine, some don't. Generally your chances are best if the protein is sturdy. Is also depends how it was lyophilized (any salts, buffer etc). Getting the protein in solution may be tricky; in my limited experience plain water works best (not buffered). Good luck, Bert On 5/6/11 4:01 PM, REX PALMER rex.pal...@btinternet.com wrote: We have purchased a 5mg commercial sample of a protein we want to crystallize. On arrival it transpired that the sample had been lyophilized. Does anyone know if this is likely to give problems and if so what can/should be done about it? Thanks in advance Rex Palmer Birkbeck College
