Re: [ccp4bb] S-nitrosylation protein
Dear Uma, A very complehensive survey of the state-of-the-art in this topic can be found in the following literature resources: (1) Garman EF. Radiation damage in macromolecular crystallography: what is it and why should we care? Acta Crystallogr D Biol Crystallogr. 2010 Apr;66(Pt 4):339-51. (2) The January 2013 issue of the Journal of Synchrotron Radiation featuring a special section with papers presented at last year's Intl workshop on X-ray damage of biological crystalline samples. The annotated editorial by Garman and Weik in that issue is also very helpful. Best regards Savvas On 13 Feb 2013, at 23:38, Uma Ratu wrote: Dear All: I plan to use X-ray crystallography method to study the S-nitrosylated protein structure. The native protein crystals diffracted to 2A with synchrontron. I now have the crystals of S-ntrosylated protein. Since S-NO moiety appears to be unstable to synchrotron radiation, could you advice / comments on the stratage on the data collection of S-nitrosylated protein crystals? The protein crystals did not diffract well with in house X-ray. Thank you for your comments. Uma
Re: [ccp4bb] S-nitrosylation protein
Collect small slices of data (instead of a complete data set) on several crystals then merge the data together to get a full data set. The slices of must be small enough so that the damage to the S-NO group is still very limited on each slice. You may have to play with beam attenuation a bit, depending on how fast the degradation occurs. Good luck Thierry From: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] On Behalf Of Uma Ratu Sent: Wednesday, February 13, 2013 5:38 PM To: CCP4BB@JISCMAIL.AC.UK Subject: [ccp4bb] S-nitrosylation protein Dear All: I plan to use X-ray crystallography method to study the S-nitrosylated protein structure. The native protein crystals diffracted to 2A with synchrontron. I now have the crystals of S-ntrosylated protein. Since S-NO moiety appears to be unstable to synchrotron radiation, could you advice / comments on the stratage on the data collection of S-nitrosylated protein crystals? The protein crystals did not diffract well with in house X-ray. Thank you for your comments. Uma Notice: This e-mail message, together with any attachments, contains information of Merck Co., Inc. (One Merck Drive, Whitehouse Station, New Jersey, USA 08889), and/or its affiliates Direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system.
Re: [ccp4bb] S-nitrosylation protein
Dear All: Many thanks for your commoents and advice. I will keep them in mind. Uma On Thu, Feb 14, 2013 at 7:54 AM, Fischmann, Thierry thierry.fischm...@merck.com wrote: Collect small slices of data (instead of a complete data set) on several crystals then merge the data together to get a full data set. The slices of must be small enough so that the damage to the S-NO group is still very limited on each slice. You may have to play with beam attenuation a bit, depending on how fast the degradation occurs. ** ** Good luck ** ** Thierry ** ** *From:* CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] *On Behalf Of *Uma Ratu *Sent:* Wednesday, February 13, 2013 5:38 PM *To:* CCP4BB@JISCMAIL.AC.UK *Subject:* [ccp4bb] S-nitrosylation protein ** ** Dear All: I plan to use X-ray crystallography method to study the S-nitrosylated protein structure. The native protein crystals diffracted to 2A with synchrontron. I now have the crystals of S-ntrosylated protein. Since S-NO moiety appears to be unstable to synchrotron radiation, could you advice / comments on the stratage on the data collection of S-nitrosylated protein crystals? The protein crystals did not diffract well with in house X-ray. Thank you for your comments. Uma Notice: This e-mail message, together with any attachments, contains information of Merck Co., Inc. (One Merck Drive, Whitehouse Station, New Jersey, USA 08889), and/or its affiliates Direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system.
[ccp4bb] S-nitrosylation protein
Dear All: I plan to use X-ray crystallography method to study the S-nitrosylated protein structure. The native protein crystals diffracted to 2A with synchrontron. I now have the crystals of S-ntrosylated protein. Since S-NO moiety appears to be unstable to synchrotron radiation, could you advice / comments on the stratage on the data collection of S-nitrosylated protein crystals? The protein crystals did not diffract well with in house X-ray. Thank you for your comments. Uma
Re: [ccp4bb] S-nitrosylation protein
Maybe you can try different energies hoping that damage is wavelength dependent. It must be dose dependent though, so you may consider merging short sweeps from multiple crystals. Original message From: Uma Ratu rosiso2...@gmail.com Date: To: CCP4BB@JISCMAIL.AC.UK Subject: [ccp4bb] S-nitrosylation protein Dear All: I plan to use X-ray crystallography method to study the S-nitrosylated protein structure. The native protein crystals diffracted to 2A with synchrontron. I now have the crystals of S-ntrosylated protein. Since S-NO moiety appears to be unstable to synchrotron radiation, could you advice / comments on the stratage on the data collection of S-nitrosylated protein crystals? The protein crystals did not diffract well with in house X-ray. Thank you for your comments. Uma
