Re: [ccp4bb] best strategy to establish stable expression in mammalian cells

[John K Lee]

Hi Jerry,

Perhaps this Nature protocols paper from the Stroud Lab at UCSF might be useful.

Overexpressing human membrane proteins in stably transfected and clonal human 
embryonic kidney 293S cells


http://www.nature.com/nprot/journal/v7/n3/full/nprot.2011.453.html



-john


On Aug 17, 2012, at 3:38 PM, Jerry McCully wrote:

> Dear All;
> 
>We are planning to establish stable CHO or 293 cell clones to express 
> some receptors.
> 
> I heard that it is time consuming to select high expression clones 
> using pcDNA3.1 vectors. 
> 
>   As our crystallographer are dealing with all kinds of proteins, can 
> anyone recommend a best strategy for mammalian cell expression?
> 
>Thank you so much and have a nice weekend,
> 
> Jerry McCully
> 

[ccp4bb] best strategy to establish stable expression in mammalian cells

[Jerry McCully]

Dear All;

   We are planning to establish stable CHO or 293 cell clones to express 
some receptors.

I heard that it is time consuming to select high expression clones 
using pcDNA3.1 vectors. 

  As our crystallographer are dealing with all kinds of proteins, can 
anyone recommend a best strategy for mammalian cell expression?

   Thank you so much and have a nice weekend,

Jerry McCully