Re: [ccp4bb] need some suggestions for crystallization
Does that CA have a metal center (I think they all do)? If so, doesn't the citrate compete for the metal? JPK On Mon, Feb 4, 2013 at 2:25 PM, Roger Rowlett rrowl...@colgate.edu wrote: Human carbonic anhdyrase II can be easily crystallized from 1.3 M sodium citrate/0.1 M TrisCl pH 8.5 at 10 mg/mL protein concentration. Crystals are P21 and easily diffract to beyond 2.0 A on a home source. We cryopreserve in ML + 30% glucose. Sulfonamide ligands are easy to soak into the crystals in a few minutes during cryopreservation, and provide teaching opportunities for protein-ligand model building and refinement. Cheers, __**_ Roger Rowlett Gordon Dorothy Kline Professor Department of Chemistry Colgate University 13 Oak Drive Hamilton, NY 13346 tel: (315)-228-7245 ofc: (315)-228-7395 fax: (315)-228-7935 email: rrowl...@colgate.edu On 02/04/2013 12:31 PM, Jim Pflugrath wrote: A number of protein crystal recipes are available on the Rigaku web site: http://www.rigaku.com/**products/protein/recipeshttp://www.rigaku.com/products/protein/recipes Lysozyme is nice because it is so cheap, grows quickly, cryoprotectants in a straightforward way, and the unit cells are not large nor are the crystals fragile. One can get triclinic, monoclinic, orthorhombic, and tetragonal crystals relatively quickly. With hen egg white lysozyme if you are not up for sulfur-SAD phasing, then co-crystallizing or quick-soaking in iodide (say 100 mM) gives a very nice anomalous signal at just about any wavelength. That is, no need to worry about going to a so-called edge. While there are many other easy-to-go crystals, I have found that none of them combine all the properties of hen egg white lysozyme has a good teaching tool. Jim == Can you all suggest some protein crystallization conditions (along with cryo conditions) for some commercially available proteins? I'm looking to get 6-8 different ones (and we'll just take them and see how it goes). I wouldn't mind knowing unit cell parameters as well (just a citation works, I can have them figure it out). I have about 7 weeks to get everything grown and frozen and ready to go. Any help would be greatly appreciated. It always amazes me how helpful this group is. Thank you very much. Dave -- *** Jacob Pearson Keller, PhD Postdoctoral Associate HHMI Janelia Farms Research Campus email: j-kell...@northwestern.edu ***
[ccp4bb] need some suggestions for crystallization
So, I know I say this every time I post on this board, but here it goes again. I'm at an undergrad only school, and every 2 years I teach a class in protein crystallography. This year I'm being super ambitious, and I'm going to take a class of 16 to the synchrotron for data collection. It's just an 8 hour thing, to show them the entire process. I'm hoping that we can collect 5-6 good data sets while there. I would like them to grow their own crystals, and go collect data. Then we'd come back and actually do a molecular replacement (pretty easy/standard really). Just to get a feel for how it works. The protein I do research on is not one that I would push on this, as the crystals are hard to grow, they are very soft, and the data just isn't the best (resolution issues). I do have a few that will work on my proteins, but I was thinking of having others in the class grow up classic proteins for data collection. Obviously lysozyme is one, but I was wondering what other standard bulletproof conditions are out there. Can you all suggest some protein crystallization conditions (along with cryo conditions) for some commercially available proteins? I'm looking to get 6-8 different ones (and we'll just take them and see how it goes). I wouldn't mind knowing unit cell parameters as well (just a citation works, I can have them figure it out). I have about 7 weeks to get everything grown and frozen and ready to go. Any help would be greatly appreciated. It always amazes me how helpful this group is. Thank you very much. Dave
Re: [ccp4bb] need some suggestions for crystallization
Hi David try going back to the one that started it all,* myoglobin, a recipe is at http://www.rigaku.com/products/protein/recipes (* feel free to argue about this) On 4 Feb 2013, at Mon4 Feb 16:03, David Roberts wrote: So, I know I say this every time I post on this board, but here it goes again. I'm at an undergrad only school, and every 2 years I teach a class in protein crystallography. This year I'm being super ambitious, and I'm going to take a class of 16 to the synchrotron for data collection. It's just an 8 hour thing, to show them the entire process. I'm hoping that we can collect 5-6 good data sets while there. I would like them to grow their own crystals, and go collect data. Then we'd come back and actually do a molecular replacement (pretty easy/standard really). Just to get a feel for how it works. The protein I do research on is not one that I would push on this, as the crystals are hard to grow, they are very soft, and the data just isn't the best (resolution issues). I do have a few that will work on my proteins, but I was thinking of having others in the class grow up classic proteins for data collection. Obviously lysozyme is one, but I was wondering what other standard bulletproof conditions are out there. Can you all suggest some protein crystallization conditions (along with cryo conditions) for some commercially available proteins? I'm looking to get 6-8 different ones (and we'll just take them and see how it goes). I wouldn't mind knowing unit cell parameters as well (just a citation works, I can have them figure it out). I have about 7 weeks to get everything grown and frozen and ready to go. Any help would be greatly appreciated. It always amazes me how helpful this group is. Thank you very much. Dave Harry -- Dr Harry Powell, MRC Laboratory of Molecular Biology, MRC Centre, Hills Road, Cambridge, CB2 0QH Chairman of European Crystallographic Association SIG9 (Crystallographic Computing)
Re: [ccp4bb] need some suggestions for crystallization
Dear Powell, Isn't it there a way to data mine the PDB or the other repository source for the time/duration/days of the crystals obtained. Dr. Jayashankar Selvadurai Hannover Germany On Mon, Feb 4, 2013 at 5:10 PM, Harry Powell ha...@mrc-lmb.cam.ac.ukwrote: Hi David try going back to the one that started it all,* myoglobin, a recipe is at http://www.rigaku.com/products/protein/recipes (* feel free to argue about this) On 4 Feb 2013, at Mon4 Feb 16:03, David Roberts wrote: So, I know I say this every time I post on this board, but here it goes again. I'm at an undergrad only school, and every 2 years I teach a class in protein crystallography. This year I'm being super ambitious, and I'm going to take a class of 16 to the synchrotron for data collection. It's just an 8 hour thing, to show them the entire process. I'm hoping that we can collect 5-6 good data sets while there. I would like them to grow their own crystals, and go collect data. Then we'd come back and actually do a molecular replacement (pretty easy/standard really). Just to get a feel for how it works. The protein I do research on is not one that I would push on this, as the crystals are hard to grow, they are very soft, and the data just isn't the best (resolution issues). I do have a few that will work on my proteins, but I was thinking of having others in the class grow up classic proteins for data collection. Obviously lysozyme is one, but I was wondering what other standard bulletproof conditions are out there. Can you all suggest some protein crystallization conditions (along with cryo conditions) for some commercially available proteins? I'm looking to get 6-8 different ones (and we'll just take them and see how it goes). I wouldn't mind knowing unit cell parameters as well (just a citation works, I can have them figure it out). I have about 7 weeks to get everything grown and frozen and ready to go. Any help would be greatly appreciated. It always amazes me how helpful this group is. Thank you very much. Dave Harry -- Dr Harry Powell, MRC Laboratory of Molecular Biology, MRC Centre, Hills Road, Cambridge, CB2 0QH Chairman of European Crystallographic Association SIG9 (Crystallographic Computing)
Re: [ccp4bb] need some suggestions for crystallization
I don't know. Is there? On 4 Feb 2013, at Mon4 Feb 16:15, Jayashankar wrote: Dear Powell, Isn't it there a way to data mine the PDB or the other repository source for the time/duration/days of the crystals obtained. Dr. Jayashankar Selvadurai Hannover Germany On Mon, Feb 4, 2013 at 5:10 PM, Harry Powell harry@mrc- lmb.cam.ac.uk wrote: Hi David try going back to the one that started it all,* myoglobin, a recipe is at http://www.rigaku.com/products/protein/recipes (* feel free to argue about this) On 4 Feb 2013, at Mon4 Feb 16:03, David Roberts wrote: So, I know I say this every time I post on this board, but here it goes again. I'm at an undergrad only school, and every 2 years I teach a class in protein crystallography. This year I'm being super ambitious, and I'm going to take a class of 16 to the synchrotron for data collection. It's just an 8 hour thing, to show them the entire process. I'm hoping that we can collect 5-6 good data sets while there. I would like them to grow their own crystals, and go collect data. Then we'd come back and actually do a molecular replacement (pretty easy/standard really). Just to get a feel for how it works. The protein I do research on is not one that I would push on this, as the crystals are hard to grow, they are very soft, and the data just isn't the best (resolution issues). I do have a few that will work on my proteins, but I was thinking of having others in the class grow up classic proteins for data collection. Obviously lysozyme is one, but I was wondering what other standard bulletproof conditions are out there. Can you all suggest some protein crystallization conditions (along with cryo conditions) for some commercially available proteins? I'm looking to get 6-8 different ones (and we'll just take them and see how it goes). I wouldn't mind knowing unit cell parameters as well (just a citation works, I can have them figure it out). I have about 7 weeks to get everything grown and frozen and ready to go. Any help would be greatly appreciated. It always amazes me how helpful this group is. Thank you very much. Dave Harry -- Dr Harry Powell, MRC Laboratory of Molecular Biology, MRC Centre, Hills Road, Cambridge, CB2 0QH Chairman of European Crystallographic Association SIG9 (Crystallographic Computing) Harry -- Dr Harry Powell, MRC Laboratory of Molecular Biology, MRC Centre, Hills Road, Cambridge, CB2 0QH Chairman of European Crystallographic Association SIG9 (Crystallographic Computing)
Re: [ccp4bb] need some suggestions for crystallization
Hi David, You could try the Glucose Isomerase supplied by Hampton. It crystallizes under a number of conditions, details of which you can find in their manual. http://hamptonresearch.com/product_detail.aspx?cid=28sid=56pid=56 Ganesh Le 04/02/13 17:03, David Roberts a écrit : So, I know I say this every time I post on this board, but here it goes again. I'm at an undergrad only school, and every 2 years I teach a class in protein crystallography. This year I'm being super ambitious, and I'm going to take a class of 16 to the synchrotron for data collection. It's just an 8 hour thing, to show them the entire process. I'm hoping that we can collect 5-6 good data sets while there. I would like them to grow their own crystals, and go collect data. Then we'd come back and actually do a molecular replacement (pretty easy/standard really). Just to get a feel for how it works. The protein I do research on is not one that I would push on this, as the crystals are hard to grow, they are very soft, and the data just isn't the best (resolution issues). I do have a few that will work on my proteins, but I was thinking of having others in the class grow up classic proteins for data collection. Obviously lysozyme is one, but I was wondering what other standard bulletproof conditions are out there. Can you all suggest some protein crystallization conditions (along with cryo conditions) for some commercially available proteins? I'm looking to get 6-8 different ones (and we'll just take them and see how it goes). I wouldn't mind knowing unit cell parameters as well (just a citation works, I can have them figure it out). I have about 7 weeks to get everything grown and frozen and ready to go. Any help would be greatly appreciated. It always amazes me how helpful this group is. Thank you very much. Dave
Re: [ccp4bb] need some suggestions for crystallization
*** This message has been scanned by the InterScan for CSC SSM by IICT security policy and found to be free of known security risks. *** Dave, You can try any or all of these proteins commercially available and all conditions for crystallization and freezing established. LysozymeFerritinGlucose isomerase Myoglobin Proteinase KThaumatin Trypsin You can follow this link. We used some of the conditions mentioned in their successfully. http://www.rigaku.com/products/protein/recipes Disclaimer: I do not have any commercial interest with Rigaku. Anthony - Dr. Anthony Addlagatta Center for Chemical Biology Indian Institute of Chemical Technology [IICT] Tarnaka, Hyderabad AP-500 607, INDIA Tel:91-40-27191812 Web: https://sites.google.com/site/chembioliict/home/dr-anthony-addlagatta-1 -- Original Message --- From: Harry Powell ha...@mrc-lmb.cam.ac.uk To: CCP4BB@JISCMAIL.AC.UK Sent: Mon, 4 Feb 2013 16:10:54 + Subject: Re: [ccp4bb] need some suggestions for crystallization *** This message has been scanned by the InterScan for CSC SSM at IICT and found to be free of known security risks. *** Hi David try going back to the one that started it all,* myoglobin, a recipe is at http://www.rigaku.com/products/protein/recipes (* feel free to argue about this) On 4 Feb 2013, at Mon4 Feb 16:03, David Roberts wrote: So, I know I say this every time I post on this board, but here it goes again. I'm at an undergrad only school, and every 2 years I teach a class in protein crystallography. This year I'm being super ambitious, and I'm going to take a class of 16 to the synchrotron for data collection. It's just an 8 hour thing, to show them the entire process. I'm hoping that we can collect 5-6 good data sets while there. I would like them to grow their own crystals, and go collect data. Then we'd come back and actually do a molecular replacement (pretty easy/standard really). Just to get a feel for how it works. The protein I do research on is not one that I would push on this, as the crystals are hard to grow, they are very soft, and the data just isn't the best (resolution issues). I do have a few that will work on my proteins, but I was thinking of having others in the class grow up classic proteins for data collection. Obviously lysozyme is one, but I was wondering what other standard bulletproof conditions are out there. Can you all suggest some protein crystallization conditions (along with cryo conditions) for some commercially available proteins? I'm looking to get 6-8 different ones (and we'll just take them and see how it goes). I wouldn't mind knowing unit cell parameters as well (just a citation works, I can have them figure it out). I have about 7 weeks to get everything grown and frozen and ready to go. Any help would be greatly appreciated. It always amazes me how helpful this group is. Thank you very much. Dave Harry -- Dr Harry Powell, MRC Laboratory of Molecular Biology, MRC Centre, Hills Road, Cambridge, CB2 0QH Chairman of European Crystallographic Association SIG9 (Crystallographic Computing) --- End of Original Message --- This Mail Scanned by ClamAV and Spammassassin
Re: [ccp4bb] need some suggestions for crystallization
My suggestions would be to look up citations for thaumatin and glucose isomerase. If I remember correctly, both of them form well diffracting crystals within a short period of time. I think you can also buy the purified protein from a vendor. Perhaps you could also try the good old lysozyme. Cheers, Raji On Mon, Feb 4, 2013 at 11:03 AM, David Roberts drobe...@depauw.edu wrote: So, I know I say this every time I post on this board, but here it goes again. I'm at an undergrad only school, and every 2 years I teach a class in protein crystallography. This year I'm being super ambitious, and I'm going to take a class of 16 to the synchrotron for data collection. It's just an 8 hour thing, to show them the entire process. I'm hoping that we can collect 5-6 good data sets while there. I would like them to grow their own crystals, and go collect data. Then we'd come back and actually do a molecular replacement (pretty easy/standard really). Just to get a feel for how it works. The protein I do research on is not one that I would push on this, as the crystals are hard to grow, they are very soft, and the data just isn't the best (resolution issues). I do have a few that will work on my proteins, but I was thinking of having others in the class grow up classic proteins for data collection. Obviously lysozyme is one, but I was wondering what other standard bulletproof conditions are out there. Can you all suggest some protein crystallization conditions (along with cryo conditions) for some commercially available proteins? I'm looking to get 6-8 different ones (and we'll just take them and see how it goes). I wouldn't mind knowing unit cell parameters as well (just a citation works, I can have them figure it out). I have about 7 weeks to get everything grown and frozen and ready to go. Any help would be greatly appreciated. It always amazes me how helpful this group is. Thank you very much. Dave -- Raji Edayathumangalam Instructor in Neurology, Harvard Medical School Research Associate, Brigham and Women's Hospital Visiting Research Scholar, Brandeis University
Re: [ccp4bb] need some suggestions for crystallization
-BEGIN PGP SIGNED MESSAGE- Hash: SHA1 Dear Dave, many methods articles mention a small set of commonly used proteins. E.g. Mueller et al, Optimal fine phi-slicing for single-photon-counting pixel detectors, Acta Cryst D68, p42-56 list Insulin, Lysozyme, Thaumatin, and Thermolysin; Nanao et al, Improving radiation-damage substructures for RIP, Acta D61, 1227-1237, list Elastase, Insulin, Lysozyme, Ribonuclease A, Thaumatin, and Trypsin. Elastase, though, decided not to crystallise any longer about ten'ish years ago. Attached is a different condition for Lysozyme which I received from Prof. Susana Andrade and which I usually use for teaching. - the crystals grow during lunch time. They can be picked directly and you can show the cryo-protecting effect of Ethylene glycol with increasing concentration. Best, Tim On 02/04/2013 05:03 PM, David Roberts wrote: So, I know I say this every time I post on this board, but here it goes again. I'm at an undergrad only school, and every 2 years I teach a class in protein crystallography. This year I'm being super ambitious, and I'm going to take a class of 16 to the synchrotron for data collection. It's just an 8 hour thing, to show them the entire process. I'm hoping that we can collect 5-6 good data sets while there. I would like them to grow their own crystals, and go collect data. Then we'd come back and actually do a molecular replacement (pretty easy/standard really). Just to get a feel for how it works. The protein I do research on is not one that I would push on this, as the crystals are hard to grow, they are very soft, and the data just isn't the best (resolution issues). I do have a few that will work on my proteins, but I was thinking of having others in the class grow up classic proteins for data collection. Obviously lysozyme is one, but I was wondering what other standard bulletproof conditions are out there. Can you all suggest some protein crystallization conditions (along with cryo conditions) for some commercially available proteins? I'm looking to get 6-8 different ones (and we'll just take them and see how it goes). I wouldn't mind knowing unit cell parameters as well (just a citation works, I can have them figure it out). I have about 7 weeks to get everything grown and frozen and ready to go. Any help would be greatly appreciated. It always amazes me how helpful this group is. Thank you very much. Dave - -- - -- Dr Tim Gruene Institut fuer anorganische Chemie Tammannstr. 4 D-37077 Goettingen GPG Key ID = A46BEE1A -BEGIN PGP SIGNATURE- Version: GnuPG v1.4.12 (GNU/Linux) Comment: Using GnuPG with Mozilla - http://enigmail.mozdev.org/ iD8DBQFRD+M2UxlJ7aRr7hoRAnDhAJ0bE6xl0XDxV6qoKslThPyyVd3CwACgpjnA sIjr3296ejuwby0ZyXLw5po= =JgwT -END PGP SIGNATURE- xtalization_lysozyme.ods Description: application/vnd.oasis.opendocument.spreadsheet
Re: [ccp4bb] need some suggestions for crystallization
Bovine trypsin works well. You can buy it pretty cheap from Sigma and it crystallizes without further purification, within a week. Crystals diffract to 1.1-1.3 A and are quite robust to handling and soaking. Conditions that I used are described in this ref: http://www.pnas.org/content/103/18/6835.long I would avoid commercial preps of porcine elastase; I messed around with that one around 10 years ago using many reported crystallization conditions and suppliers but never got crystals. Evette S. Radisky, Ph.D. Assistant Professor Mayo Clinic Cancer Center Griffin Cancer Research Building, Rm 310 4500 San Pablo Road Jacksonville, FL 32224 (904) 953-6372 -Original Message- From: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] On Behalf Of David Roberts Sent: Monday, February 04, 2013 11:03 AM To: CCP4BB@JISCMAIL.AC.UK Subject: [ccp4bb] need some suggestions for crystallization So, I know I say this every time I post on this board, but here it goes again. I'm at an undergrad only school, and every 2 years I teach a class in protein crystallography. This year I'm being super ambitious, and I'm going to take a class of 16 to the synchrotron for data collection. It's just an 8 hour thing, to show them the entire process. I'm hoping that we can collect 5-6 good data sets while there. I would like them to grow their own crystals, and go collect data. Then we'd come back and actually do a molecular replacement (pretty easy/standard really). Just to get a feel for how it works. The protein I do research on is not one that I would push on this, as the crystals are hard to grow, they are very soft, and the data just isn't the best (resolution issues). I do have a few that will work on my proteins, but I was thinking of having others in the class grow up classic proteins for data collection. Obviously lysozyme is one, but I was wondering what other standard bulletproof conditions are out there. Can you all suggest some protein crystallization conditions (along with cryo conditions) for some commercially available proteins? I'm looking to get 6-8 different ones (and we'll just take them and see how it goes). I wouldn't mind knowing unit cell parameters as well (just a citation works, I can have them figure it out). I have about 7 weeks to get everything grown and frozen and ready to go. Any help would be greatly appreciated. It always amazes me how helpful this group is. Thank you very much. Dave
Re: [ccp4bb] need some suggestions for crystallization
I second that. Gently swirling the bottle (very important) before pipetting a few microliters (say 100 microliters or whatever). Dialyse overnight vs 10 mM HEPES 2 mM MgCl2 ph7, then get the protein concentration to 10 mg/ml. Set up the drops (sitting drops) versus the same buffer with 30% (v/v) MPD. Direct cryo conditions. Depending on the material (plastic) used for the sitting drop trays you may find it difficult to get crystals out - you'll break up a few - but then no messing up to try to find cryo-conditions. Fred. On 04/02/13 17:27, Ganesh Natrajan wrote: Hi David, You could try the Glucose Isomerase supplied by Hampton. It crystallizes under a number of conditions, details of which you can find in their manual. http://hamptonresearch.com/product_detail.aspx?cid=28sid=56pid=56 Ganesh -- Fred. Vellieux (B.Sc., Ph.D., hdr) IBS / ELMA 41 rue Jules Horowitz F-38027 Grenoble Cedex 01 Tel: +33 438789605 Fax: +33 438785494
Re: [ccp4bb] need some suggestions for crystallization
Human carbonic anhdyrase II can be easily crystallized from 1.3 M sodium citrate/0.1 M TrisCl pH 8.5 at 10 mg/mL protein concentration. Crystals are P21 and easily diffract to beyond 2.0 A on a home source. We cryopreserve in ML + 30% glucose. Sulfonamide ligands are easy to soak into the crystals in a few minutes during cryopreservation, and provide teaching opportunities for protein-ligand model building and refinement. Cheers, ___ Roger Rowlett Gordon Dorothy Kline Professor Department of Chemistry Colgate University 13 Oak Drive Hamilton, NY 13346 tel: (315)-228-7245 ofc: (315)-228-7395 fax: (315)-228-7935 email: rrowl...@colgate.edu On 02/04/2013 12:31 PM, Jim Pflugrath wrote: A number of protein crystal recipes are available on the Rigaku web site: http://www.rigaku.com/products/protein/recipes Lysozyme is nice because it is so cheap, grows quickly, cryoprotectants in a straightforward way, and the unit cells are not large nor are the crystals fragile. One can get triclinic, monoclinic, orthorhombic, and tetragonal crystals relatively quickly. With hen egg white lysozyme if you are not up for sulfur-SAD phasing, then co-crystallizing or quick-soaking in iodide (say 100 mM) gives a very nice anomalous signal at just about any wavelength. That is, no need to worry about going to a so-called edge. While there are many other easy-to-go crystals, I have found that none of them combine all the properties of hen egg white lysozyme has a good teaching tool. Jim == Can you all suggest some protein crystallization conditions (along with cryo conditions) for some commercially available proteins? I'm looking to get 6-8 different ones (and we'll just take them and see how it goes). I wouldn't mind knowing unit cell parameters as well (just a citation works, I can have them figure it out). I have about 7 weeks to get everything grown and frozen and ready to go. Any help would be greatly appreciated. It always amazes me how helpful this group is. Thank you very much. Dave
Re: [ccp4bb] need some suggestions for crystallization
Hi Dave, ProteinaseK is also a good one. Crystallizes rapidly, big crystals, and relatively high resolution data (1.0-1.5A) usually. You can also buy the lyophilized powder from sigma and prepare the sample directly from the commercial material. We use proK for a course here at UCLA, so if you are interested I can send you more details about the protocol. Mike - Original Message - From: David Roberts drobe...@depauw.edu To: CCP4BB@JISCMAIL.AC.UK Sent: Monday, February 4, 2013 8:03:09 AM GMT -08:00 US/Canada Pacific Subject: [ccp4bb] need some suggestions for crystallization So, I know I say this every time I post on this board, but here it goes again. I'm at an undergrad only school, and every 2 years I teach a class in protein crystallography. This year I'm being super ambitious, and I'm going to take a class of 16 to the synchrotron for data collection. It's just an 8 hour thing, to show them the entire process. I'm hoping that we can collect 5-6 good data sets while there. I would like them to grow their own crystals, and go collect data. Then we'd come back and actually do a molecular replacement (pretty easy/standard really). Just to get a feel for how it works. The protein I do research on is not one that I would push on this, as the crystals are hard to grow, they are very soft, and the data just isn't the best (resolution issues). I do have a few that will work on my proteins, but I was thinking of having others in the class grow up classic proteins for data collection. Obviously lysozyme is one, but I was wondering what other standard bulletproof conditions are out there. Can you all suggest some protein crystallization conditions (along with cryo conditions) for some commercially available proteins? I'm looking to get 6-8 different ones (and we'll just take them and see how it goes). I wouldn't mind knowing unit cell parameters as well (just a citation works, I can have them figure it out). I have about 7 weeks to get everything grown and frozen and ready to go. Any help would be greatly appreciated. It always amazes me how helpful this group is. Thank you very much. Dave -- Michael C. Thompson Graduate Student Biochemistry Molecular Biology Division Department of Chemistry Biochemistry University of California, Los Angeles mi...@chem.ucla.edu
