Re: [ccp4bb] freezing in ethane / propane - unipucks ?
Dear Guenter, Thank Dom for mentioning the CombiPuck https://www.mitegen.com/product/combipuck-system/#cs-content , which is complimentary to other types of pucks (CombiPuck can be used at beamlines which use the UniPuck format) and also makes easier to transfer crystals among different types of pucks with or without vials. Due to vial protection, it allows a bit longer time of puck handling under liquid N2 to keep the low temperature environment for the crystals. It seems to freeze the crystals well and have less chance to get crystals iced up (especially benefit for sensitive crystals) after crystal harvesting and shipment according to users’ feedback. If you happen to use the CombiPuck to ship your crystals, had better send a CombiPuck pusher https://www.mitegen.com/product/puck-pusher/ along with the shipping dewar and let the beamline staff know. It will help take out the CombiPuck transport canister easier from the shipping dewar at synchrotron because one hole and a groove on the puck pusher may align with two rods on the canister to get two rods squeezed to pull the canister out. Minmin On Wednesday, January 26, 2022, 5:26 pm, Guenter Fritz wrote: Dear Dom, thanks a lot. Yes, this might work sending a combipuck alongside with a good bottle for the local contact. I was wondering whether the grippers can handle a block of propane? Best wishes, guenter > Dear Guenter, > > Would the use of vials inside combi-pucks > (https://www.mitegen.com/product/combipuck-system/) and some > arrangements with your local contact at the other end, perhaps help > with using propane remotely? > > BW, > > D > > On 26/01/2022 16:53, Guenter Fritz wrote: >> Dear all, >> >> we have some delicate crystals which might benefit from freezing in >> propane. In former times (when I was still travelling to the >> beamlines) we waited until the propane was solid in the vial and then >> let the propane thaw in the cryo stream at the beamline. >> >> But how can we do this in these days with unipucks and no manual >> mounting? >> >> I was thinking about freezing in ethane and then transfer the loops >> to liquid nitrogen, similarly we handle grids for cryo EM. Does >> somebody has tried that? Any experience, tips & tricks would be very >> welcome! Thanks in advance and best regards, >> >> Guenter >> >> >> >> To unsubscribe from the CCP4BB list, click the following link: >> https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB=1 >> >> This message was issued to members of www.jiscmail.ac.uk/CCP4BB, a >> mailing list hosted by www.jiscmail.ac.uk, terms & conditions are >> available at https://www.jiscmail.ac.uk/policyandsecurity/ > To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB=1 This message was issued to members of www.jiscmail.ac.uk/CCP4BB, a mailing list hosted by www.jiscmail.ac.uk, terms & conditions are available at https://www.jiscmail.ac.uk/policyandsecurity/ To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB=1 This message was issued to members of www.jiscmail.ac.uk/CCP4BB, a mailing list hosted by www.jiscmail.ac.uk, terms & conditions are available at https://www.jiscmail.ac.uk/policyandsecurity/
Re: [ccp4bb] [EXTERNAL] Re: [ccp4bb] freezing in ethane / propane - unipucks ?
Hi Steve, Hi , 1) If you are collecting the data at the APS the samples frozen in propane can be legally shipped there (check APS web site for information) 2) The samples CANNOT be mounted using either the ACTOR or ALS Style auto mounter. But the samples can be shipped using either the Rigaku pucks or the Uni-pucks with a vial cover adapter. In any case you would need to manually mount and collect data or have the samples retrieved after the propane melted with the robotic mounter and mounted later by the robot for data collection. I am in Europe, so I would go for ESRF / SLS. From my extensive experience quenching samples using propane it has been possible to freeze ALL in LN2 after a) perfecting cryoprotectant, b) perfecting freezing technique, c) modifying crystal growth conditions. :)) yes better crystals is always the best solution. But we are happy that we have crystals at all after some efforts (insect cells, low solubility, crystallization in the glove box, ) Cheers Guenter Steve Ginell On Jan 26, 2022, at 1:31 PM, Joseph Ferrara wrote: When using Xe derivatization for SAD phasing, we (mostly Jim Pflugrath) used carbon tetrafluoride. CF4 has a melting point of 89.5K, just four degrees warmer than propane and is not flammable. Joe Ferrara -Original Message- From: CCP4 bulletin board On Behalf Of James Holton Sent: Wednesday, January 26, 2022 13:07 To: CCP4BB@JISCMAIL.AC.UK Subject: [EXTERNAL] Re: [ccp4bb] freezing in ethane / propane - unipucks ? I think the issue with propane at at least some light sources is that it is flammable. Makes shipping and safety more complicated. How hard would it be to let the propane melt off in a cryo gas stream at your home lab? Then use tongs to transfer the pin into liquid N2 for handling as usual? If you don't have a working N2 gas stream and are on a budget they are not that hard to build: https://doi.org/10.1107/S0021889894006357 Cheers, -James Holton MAD Scientist On 1/26/2022 9:25 AM, Guenter Fritz wrote: Dear Dom, thanks a lot. Yes, this might work sending a combipuck alongside with a good bottle for the local contact. I was wondering whether the grippers can handle a block of propane? Best wishes, guenter Dear Guenter, Would the use of vials inside combi-pucks (https://www.mitegen.com/product/combipuck-system/) and some arrangements with your local contact at the other end, perhaps help with using propane remotely? BW, D On 26/01/2022 16:53, Guenter Fritz wrote: Dear all, we have some delicate crystals which might benefit from freezing in propane. In former times (when I was still travelling to the beamlines) we waited until the propane was solid in the vial and then let the propane thaw in the cryo stream at the beamline. But how can we do this in these days with unipucks and no manual mounting? I was thinking about freezing in ethane and then transfer the loops to liquid nitrogen, similarly we handle grids for cryo EM. Does somebody has tried that? Any experience, tips & tricks would be very welcome! Thanks in advance and best regards, Guenter To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB=1 This message was issued to members of www.jiscmail.ac.uk/CCP4BB, a mailing list hosted by www.jiscmail.ac.uk, terms & conditions are available at https://www.jiscmail.ac.uk/policyandsecurity/ ## ## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB=1 This message was issued to members of www.jiscmail.ac.uk/CCP4BB, a mailing list hosted by www.jiscmail.ac.uk, terms & conditions are available at https://www.jiscmail.ac.uk/policyandsecurity/ To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB=1 This message was issued to members of www.jiscmail.ac.uk/CCP4BB, a mailing list hosted by www.jiscmail.ac.uk, terms & conditions are available at https://www.jiscmail.ac.uk/policyandsecurity/ To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB=1 This message was issued to members of www.jiscmail.ac.uk/CCP4BB, a mailing list hosted by www.jiscmail.ac.uk, terms & conditions are available at https://www.jiscmail.ac.uk/policyandsecurity/ To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB=1 This message was issued to members of www.jiscmail.ac.uk/CCP4BB, a
Re: [ccp4bb] [EXTERNAL] Re: [ccp4bb] freezing in ethane / propane - unipucks ?
Hi Joe, thanks. I have never used CF4. Good point and worth a try. Best, Guenter When using Xe derivatization for SAD phasing, we (mostly Jim Pflugrath) used carbon tetrafluoride. CF4 has a melting point of 89.5K, just four degrees warmer than propane and is not flammable. Joe Ferrara -Original Message- From: CCP4 bulletin board On Behalf Of James Holton Sent: Wednesday, January 26, 2022 13:07 To: CCP4BB@JISCMAIL.AC.UK Subject: [EXTERNAL] Re: [ccp4bb] freezing in ethane / propane - unipucks ? I think the issue with propane at at least some light sources is that it is flammable. Makes shipping and safety more complicated. How hard would it be to let the propane melt off in a cryo gas stream at your home lab? Then use tongs to transfer the pin into liquid N2 for handling as usual? If you don't have a working N2 gas stream and are on a budget they are not that hard to build: https://doi.org/10.1107/S0021889894006357 Cheers, -James Holton MAD Scientist On 1/26/2022 9:25 AM, Guenter Fritz wrote: Dear Dom, thanks a lot. Yes, this might work sending a combipuck alongside with a good bottle for the local contact. I was wondering whether the grippers can handle a block of propane? Best wishes, guenter Dear Guenter, Would the use of vials inside combi-pucks (https://www.mitegen.com/product/combipuck-system/) and some arrangements with your local contact at the other end, perhaps help with using propane remotely? BW, D On 26/01/2022 16:53, Guenter Fritz wrote: Dear all, we have some delicate crystals which might benefit from freezing in propane. In former times (when I was still travelling to the beamlines) we waited until the propane was solid in the vial and then let the propane thaw in the cryo stream at the beamline. But how can we do this in these days with unipucks and no manual mounting? I was thinking about freezing in ethane and then transfer the loops to liquid nitrogen, similarly we handle grids for cryo EM. Does somebody has tried that? Any experience, tips & tricks would be very welcome! Thanks in advance and best regards, Guenter To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB=1 This message was issued to members of www.jiscmail.ac.uk/CCP4BB, a mailing list hosted by www.jiscmail.ac.uk, terms & conditions are available at https://www.jiscmail.ac.uk/policyandsecurity/ ## ## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB=1 This message was issued to members of www.jiscmail.ac.uk/CCP4BB, a mailing list hosted by www.jiscmail.ac.uk, terms & conditions are available at https://www.jiscmail.ac.uk/policyandsecurity/ To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB=1 This message was issued to members of www.jiscmail.ac.uk/CCP4BB, a mailing list hosted by www.jiscmail.ac.uk, terms & conditions are available at https://www.jiscmail.ac.uk/policyandsecurity/ To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB=1 This message was issued to members of www.jiscmail.ac.uk/CCP4BB, a mailing list hosted by www.jiscmail.ac.uk, terms & conditions are available at https://www.jiscmail.ac.uk/policyandsecurity/ To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB=1 This message was issued to members of www.jiscmail.ac.uk/CCP4BB, a mailing list hosted by www.jiscmail.ac.uk, terms & conditions are available at https://www.jiscmail.ac.uk/policyandsecurity/
Re: [ccp4bb] freezing in ethane / propane - unipucks ?
Hi James, I think the issue with propane at at least some light sources is that it is flammable. Makes shipping and safety more complicated. How hard would it be to let the propane melt off in a cryo gas stream at your home lab? Then use tongs to transfer the pin into liquid N2 for handling as usual? If you don't have a working N2 gas stream and are on a budget they are not that hard to build: https://doi.org/10.1107/S0021889894006357 Yes, that would be straightforward, but was looking for a kind of shortcut :). Cheers Guenter Cheers, -James Holton MAD Scientist On 1/26/2022 9:25 AM, Guenter Fritz wrote: Dear Dom, thanks a lot. Yes, this might work sending a combipuck alongside with a good bottle for the local contact. I was wondering whether the grippers can handle a block of propane? Best wishes, guenter Dear Guenter, Would the use of vials inside combi-pucks (https://www.mitegen.com/product/combipuck-system/) and some arrangements with your local contact at the other end, perhaps help with using propane remotely? BW, D On 26/01/2022 16:53, Guenter Fritz wrote: Dear all, we have some delicate crystals which might benefit from freezing in propane. In former times (when I was still travelling to the beamlines) we waited until the propane was solid in the vial and then let the propane thaw in the cryo stream at the beamline. But how can we do this in these days with unipucks and no manual mounting? I was thinking about freezing in ethane and then transfer the loops to liquid nitrogen, similarly we handle grids for cryo EM. Does somebody has tried that? Any experience, tips & tricks would be very welcome! Thanks in advance and best regards, Guenter To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB=1 This message was issued to members of www.jiscmail.ac.uk/CCP4BB, a mailing list hosted by www.jiscmail.ac.uk, terms & conditions are available at https://www.jiscmail.ac.uk/policyandsecurity/ To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB=1 This message was issued to members of www.jiscmail.ac.uk/CCP4BB, a mailing list hosted by www.jiscmail.ac.uk, terms & conditions are available at https://www.jiscmail.ac.uk/policyandsecurity/ To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB=1 This message was issued to members of www.jiscmail.ac.uk/CCP4BB, a mailing list hosted by www.jiscmail.ac.uk, terms & conditions are available at https://www.jiscmail.ac.uk/policyandsecurity/
Re: [ccp4bb] [EXTERNAL] Re: [ccp4bb] freezing in ethane / propane - unipucks ?
Hi , 1) If you are collecting the data at the APS the samples frozen in propane can be legally shipped there (check APS web site for information) 2) The samples CANNOT be mounted using either the ACTOR or ALS Style auto mounter. But the samples can be shipped using either the Rigaku pucks or the Uni-pucks with a vial cover adapter. In any case you would need to manually mount and collect data or have the samples retrieved after the propane melted with the robotic mounter and mounted later by the robot for data collection. >From my extensive experience quenching samples using propane it has been >possible to freeze ALL in LN2 after a) perfecting cryoprotectant, b) >perfecting freezing technique, c) modifying crystal growth conditions. Steve Ginell > On Jan 26, 2022, at 1:31 PM, Joseph Ferrara wrote: > > When using Xe derivatization for SAD phasing, we (mostly Jim Pflugrath) used > carbon tetrafluoride. CF4 has a melting point of 89.5K, just four degrees > warmer than propane and is not flammable. > > Joe Ferrara > > -Original Message- > From: CCP4 bulletin board On Behalf Of James Holton > Sent: Wednesday, January 26, 2022 13:07 > To: CCP4BB@JISCMAIL.AC.UK > Subject: [EXTERNAL] Re: [ccp4bb] freezing in ethane / propane - unipucks ? > > I think the issue with propane at at least some light sources is that it is > flammable. Makes shipping and safety more complicated. > > How hard would it be to let the propane melt off in a cryo gas stream at your > home lab? Then use tongs to transfer the pin into liquid N2 for handling as > usual? If you don't have a working N2 gas stream and are on a budget they > are not that hard to build: > https://doi.org/10.1107/S0021889894006357 > > Cheers, > > -James Holton > MAD Scientist > > > On 1/26/2022 9:25 AM, Guenter Fritz wrote: >> Dear Dom, >> >> thanks a lot. Yes, this might work sending a combipuck alongside with >> a good bottle for the local contact. >> I was wondering whether the grippers can handle a block of propane? >> >> Best wishes, >> guenter >>> Dear Guenter, >>> >>> Would the use of vials inside combi-pucks >>> (https://www.mitegen.com/product/combipuck-system/) and some >>> arrangements with your local contact at the other end, perhaps help >>> with using propane remotely? >>> >>> BW, >>> >>> D >>> >>> On 26/01/2022 16:53, Guenter Fritz wrote: >>>> Dear all, >>>> >>>> we have some delicate crystals which might benefit from freezing in >>>> propane. In former times (when I was still travelling to the >>>> beamlines) we waited until the propane was solid in the vial and >>>> then let the propane thaw in the cryo stream at the beamline. >>>> >>>> But how can we do this in these days with unipucks and no manual >>>> mounting? >>>> >>>> I was thinking about freezing in ethane and then transfer the loops >>>> to liquid nitrogen, similarly we handle grids for cryo EM. Does >>>> somebody has tried that? Any experience, tips & tricks would be very >>>> welcome! Thanks in advance and best regards, >>>> >>>> Guenter >>>> >>>> >>>> >>>> >>>> >>>> To unsubscribe from the CCP4BB list, click the following link: >>>> https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB=1 >>>> >>>> This message was issued to members of www.jiscmail.ac.uk/CCP4BB, a >>>> mailing list hosted by www.jiscmail.ac.uk, terms & conditions are >>>> available at https://www.jiscmail.ac.uk/policyandsecurity/ >>> >> >> ## >> ## >> >> To unsubscribe from the CCP4BB list, click the following link: >> https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB=1 >> >> This message was issued to members of www.jiscmail.ac.uk/CCP4BB, a >> mailing list hosted by www.jiscmail.ac.uk, terms & conditions are >> available at https://www.jiscmail.ac.uk/policyandsecurity/ > > > > To unsubscribe from the CCP4BB list, click the following link: > https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB=1 > > This message was issued to members of www.jiscmail.ac.uk/CCP4BB, a mailing > list hosted by www.jiscmail.ac.uk, terms & condit
Re: [ccp4bb] [EXTERNAL] Re: [ccp4bb] freezing in ethane / propane - unipucks ?
When using Xe derivatization for SAD phasing, we (mostly Jim Pflugrath) used carbon tetrafluoride. CF4 has a melting point of 89.5K, just four degrees warmer than propane and is not flammable. Joe Ferrara -Original Message- From: CCP4 bulletin board On Behalf Of James Holton Sent: Wednesday, January 26, 2022 13:07 To: CCP4BB@JISCMAIL.AC.UK Subject: [EXTERNAL] Re: [ccp4bb] freezing in ethane / propane - unipucks ? I think the issue with propane at at least some light sources is that it is flammable. Makes shipping and safety more complicated. How hard would it be to let the propane melt off in a cryo gas stream at your home lab? Then use tongs to transfer the pin into liquid N2 for handling as usual? If you don't have a working N2 gas stream and are on a budget they are not that hard to build: https://doi.org/10.1107/S0021889894006357 Cheers, -James Holton MAD Scientist On 1/26/2022 9:25 AM, Guenter Fritz wrote: > Dear Dom, > > thanks a lot. Yes, this might work sending a combipuck alongside with > a good bottle for the local contact. > I was wondering whether the grippers can handle a block of propane? > > Best wishes, > guenter >> Dear Guenter, >> >> Would the use of vials inside combi-pucks >> (https://www.mitegen.com/product/combipuck-system/) and some >> arrangements with your local contact at the other end, perhaps help >> with using propane remotely? >> >> BW, >> >> D >> >> On 26/01/2022 16:53, Guenter Fritz wrote: >>> Dear all, >>> >>> we have some delicate crystals which might benefit from freezing in >>> propane. In former times (when I was still travelling to the >>> beamlines) we waited until the propane was solid in the vial and >>> then let the propane thaw in the cryo stream at the beamline. >>> >>> But how can we do this in these days with unipucks and no manual >>> mounting? >>> >>> I was thinking about freezing in ethane and then transfer the loops >>> to liquid nitrogen, similarly we handle grids for cryo EM. Does >>> somebody has tried that? Any experience, tips & tricks would be very >>> welcome! Thanks in advance and best regards, >>> >>> Guenter >>> >>> >>> >>> >>> >>> To unsubscribe from the CCP4BB list, click the following link: >>> https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB=1 >>> >>> This message was issued to members of www.jiscmail.ac.uk/CCP4BB, a >>> mailing list hosted by www.jiscmail.ac.uk, terms & conditions are >>> available at https://www.jiscmail.ac.uk/policyandsecurity/ >> > > ## > ## > > To unsubscribe from the CCP4BB list, click the following link: > https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB=1 > > This message was issued to members of www.jiscmail.ac.uk/CCP4BB, a > mailing list hosted by www.jiscmail.ac.uk, terms & conditions are > available at https://www.jiscmail.ac.uk/policyandsecurity/ To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB=1 This message was issued to members of www.jiscmail.ac.uk/CCP4BB, a mailing list hosted by www.jiscmail.ac.uk, terms & conditions are available at https://www.jiscmail.ac.uk/policyandsecurity/ To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB=1 This message was issued to members of www.jiscmail.ac.uk/CCP4BB, a mailing list hosted by www.jiscmail.ac.uk, terms & conditions are available at https://www.jiscmail.ac.uk/policyandsecurity/
Re: [ccp4bb] freezing in ethane / propane - unipucks ?
I think the issue with propane at at least some light sources is that it is flammable. Makes shipping and safety more complicated. How hard would it be to let the propane melt off in a cryo gas stream at your home lab? Then use tongs to transfer the pin into liquid N2 for handling as usual? If you don't have a working N2 gas stream and are on a budget they are not that hard to build: https://doi.org/10.1107/S0021889894006357 Cheers, -James Holton MAD Scientist On 1/26/2022 9:25 AM, Guenter Fritz wrote: Dear Dom, thanks a lot. Yes, this might work sending a combipuck alongside with a good bottle for the local contact. I was wondering whether the grippers can handle a block of propane? Best wishes, guenter Dear Guenter, Would the use of vials inside combi-pucks (https://www.mitegen.com/product/combipuck-system/) and some arrangements with your local contact at the other end, perhaps help with using propane remotely? BW, D On 26/01/2022 16:53, Guenter Fritz wrote: Dear all, we have some delicate crystals which might benefit from freezing in propane. In former times (when I was still travelling to the beamlines) we waited until the propane was solid in the vial and then let the propane thaw in the cryo stream at the beamline. But how can we do this in these days with unipucks and no manual mounting? I was thinking about freezing in ethane and then transfer the loops to liquid nitrogen, similarly we handle grids for cryo EM. Does somebody has tried that? Any experience, tips & tricks would be very welcome! Thanks in advance and best regards, Guenter To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB=1 This message was issued to members of www.jiscmail.ac.uk/CCP4BB, a mailing list hosted by www.jiscmail.ac.uk, terms & conditions are available at https://www.jiscmail.ac.uk/policyandsecurity/ To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB=1 This message was issued to members of www.jiscmail.ac.uk/CCP4BB, a mailing list hosted by www.jiscmail.ac.uk, terms & conditions are available at https://www.jiscmail.ac.uk/policyandsecurity/ To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB=1 This message was issued to members of www.jiscmail.ac.uk/CCP4BB, a mailing list hosted by www.jiscmail.ac.uk, terms & conditions are available at https://www.jiscmail.ac.uk/policyandsecurity/
Re: [ccp4bb] freezing in ethane / propane - unipucks ?
Dear Dom, thanks a lot. Yes, this might work sending a combipuck alongside with a good bottle for the local contact. I was wondering whether the grippers can handle a block of propane? Best wishes, guenter Dear Guenter, Would the use of vials inside combi-pucks (https://www.mitegen.com/product/combipuck-system/) and some arrangements with your local contact at the other end, perhaps help with using propane remotely? BW, D On 26/01/2022 16:53, Guenter Fritz wrote: Dear all, we have some delicate crystals which might benefit from freezing in propane. In former times (when I was still travelling to the beamlines) we waited until the propane was solid in the vial and then let the propane thaw in the cryo stream at the beamline. But how can we do this in these days with unipucks and no manual mounting? I was thinking about freezing in ethane and then transfer the loops to liquid nitrogen, similarly we handle grids for cryo EM. Does somebody has tried that? Any experience, tips & tricks would be very welcome! Thanks in advance and best regards, Guenter To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB=1 This message was issued to members of www.jiscmail.ac.uk/CCP4BB, a mailing list hosted by www.jiscmail.ac.uk, terms & conditions are available at https://www.jiscmail.ac.uk/policyandsecurity/ To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB=1 This message was issued to members of www.jiscmail.ac.uk/CCP4BB, a mailing list hosted by www.jiscmail.ac.uk, terms & conditions are available at https://www.jiscmail.ac.uk/policyandsecurity/
Re: [ccp4bb] freezing in ethane / propane - unipucks ?
Dear Guenter, Would the use of vials inside combi-pucks (https://www.mitegen.com/product/combipuck-system/) and some arrangements with your local contact at the other end, perhaps help with using propane remotely? BW, D On 26/01/2022 16:53, Guenter Fritz wrote: Dear all, we have some delicate crystals which might benefit from freezing in propane. In former times (when I was still travelling to the beamlines) we waited until the propane was solid in the vial and then let the propane thaw in the cryo stream at the beamline. But how can we do this in these days with unipucks and no manual mounting? I was thinking about freezing in ethane and then transfer the loops to liquid nitrogen, similarly we handle grids for cryo EM. Does somebody has tried that? Any experience, tips & tricks would be very welcome! Thanks in advance and best regards, Guenter To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB=1 This message was issued to members of www.jiscmail.ac.uk/CCP4BB, a mailing list hosted by www.jiscmail.ac.uk, terms & conditions are available at https://www.jiscmail.ac.uk/policyandsecurity/ -- Dom Bellini, Xray Crystallography Facility (1S205) MRC Laboratory of Molecular Biology Francis Crick Avenue Cambridge Biomedical Campus Cambridge CB2 0QH Phone 01223 267839 To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB=1 This message was issued to members of www.jiscmail.ac.uk/CCP4BB, a mailing list hosted by www.jiscmail.ac.uk, terms & conditions are available at https://www.jiscmail.ac.uk/policyandsecurity/
