Re: [Histonet] Red chroamgen/LeicaBond andBondMax

[Cartun, Richard via Histonet]

Yes; we run IHC tests everyday on our Bonds with Leica's "Red" detection kit.  
You do need to be careful when dehydrating the slides prior to coverslipping 
since the chromogenic product is not as stable as DAB.

Richard

Richard W. Cartun, MS, PhD
Director, Histology & The Martin M. Berman, MD
  Immunopathology/Morphologic Proteomics Laboratory
Assistant Director, Anatomic Pathology
Department of Pathology & Laboratory Medicine
Hartford Hospital
80 Seymour Street
Hartford, CT  06102
(860) 972-1596 Office
(860) 545-2204 (Fax)



-Original Message-
From: John O’Brien via Histonet [mailto:histonet@lists.utsouthwestern.edu]
Sent: Thursday, June 9, 2022 1:21 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Red chroamgen/LeicaBond andBondMax

CAUTION: This email is from outside HHC. USE CARE when opening attachments or 
links.

Can some tell me if the Leica IHC Bond or Bond Max can preform the Red 
Chroamgen IHC stain?
John
IMEB

___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
https://urldefense.com/v3/__http://lists.utsouthwestern.edu/mailman/listinfo/histonet__;!!KCs9X-8!dM57DqvKTlhaZFjI21NsFvkIXuDFBoPLUmyLNGPYzxgY5eVBTEyIU-xvMenryn-kwf1k4JZgHYI4QnptD4j7Hj6p6MLk-RL81w$


Reminder: This e-mail and any attachments are subject to the current HHC email 
retention policies. Please save or store appropriately in accordance with 
policy.

This e-mail message, including any attachments, is for the sole use of the 
intended recipient(s) and may contain confidential and privileged information. 
Any unauthorized review, use, disclosure, or distribution is prohibited. If you 
are not the intended recipient, or an employee or agent responsible for 
delivering the message to the intended recipient, please contact the sender by 
reply e-mail and destroy all copies of the original message, including any 
attachments.

___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

Re: [Histonet] Histonet Digest, Vol 220, Issue 8

[Cartun, Richard via Histonet]

Well stated Tony, and thank for all this useful information.  I am hopeful that 
we can get expiration dates overturned here in the United States.  In the past, 
I have recommended a "Stability Guarantee" date to replace the "Expiration 
Date".  After that date passes it would be the responsibility of the performing 
laboratory to validate that the antibody is still working properly.  And, as 
you pointed out, this is easily accomplished with the positive control which is 
run in parallel.  Laboratories cannot no longer afford to discard expensive 
reagents, especially those that are not being produced any longer.  Also, with 
the current staffing crisis in Histology/IHC laboratories and supply-chain 
issues we need to simplify our work environment.

Richard

Richard W. Cartun, MS, PhD
Director, Histology & The Martin M. Berman, MD
  Immunopathology/Morphologic Proteomics Laboratory
Assistant Director, Anatomic Pathology
Department of Pathology & Laboratory Medicine
Hartford Hospital
80 Seymour Street
Hartford, CT  06102
(860) 972-1596 Office
(860) 545-2204 (Fax)



-Original Message-
From: Tony Henwood (SCHN) via Histonet 
[mailto:histonet@lists.utsouthwestern.edu]
Sent: Wednesday, March 16, 2022 9:18 PM
To: jayalakshmy p.s
Cc: 'histonet@lists.utsouthwestern.edu'
Subject: Re: [Histonet] Histonet Digest, Vol 220, Issue 8

EXTERNAL email from Outside HHC! Do NOT open attachments or click links from 
unknown senders.

Hi Jayalakshmy,

This our policy at the Kid's hospital in Sydney:

Validation of Expired Antibodies
Usually when a new concentrated antibody is received it will have an expiry 
date of around 2 years from receipt but usually we can continue to use 
antibodies well past this expiry date.

If the antibody continues to stain control sections appropriately, with no loss 
of sensitivity and no increase in non-specific staining then its use should be 
continued. If positive control samples are deemed unsatisfactory, even if the 
antibody is within the manufacturer’s printed expiration date, evaluation of 
the clinical specimen is aborted and the test deemed invalid. The quality of 
the primary antibody is therefore not based on an expiration date, but rather 
on its performance on a case-by-case basis with appropriate positive and 
negative control samples (1).

Several authors have investigated whether the shelf-life of diagnostic 
antibodies was longer than the expiry date on the label. They found them to 
work perfectly on routine histology sections (1-4). Monoclonal antibodies 
originally supplied as culture supernatants or as ascites (neat or diluted), of 
all isotypes, as well as all of the polyclonal antibodies, produced 
satisfactory staining irrespective of their age. Notable exceptions were 
ammonium-precipitated, IgM or conjugated antibodies.

The policy at CHW is, when an antibody has reached past its expiry date, its 
control is tested to ensure that there has been no loss of sensitivity in the 
test. This is now controlled through iPassport, where a task is attached to the 
antibody requesting validation of control when the antibody is expired. This 
can be easily done by using the History Screen and looking for use of this 
antibody within the last two weeks. If results of control are acceptable, 
another task is instigated for 6 months hence.

For antibody concentrates that are received without an expiry date, a 
verification is scheduled 12 months after receipt of the antibody.

If an antibody fails to perform to expectations than a Corrective Action 
Request is instigated in iPassport and appropriate investigation is instituted.

References:
1. Savage, E. C., & DeYoung, B. R. (2010). Antibody Expiration in the Context 
of Resource Limitation What Is the Evidence Basis?. American journal of 
clinical pathology, 134(1), 60-64.
2. Balaton, A. J., Drachenberg, C. B., Rucker, C., Vaury, P., & Papadimitriou, 
J. C. (1999). Satisfactory performance of primary antibodies beyond 
manufacturers' recommended expiration dates. Applied Immunohistochemistry & 
Molecular Morphology, 7(3), 221.
3. Argentieri, M. C., Pilla, D., Vanzati, A., Lonardi, S., Facchetti, F., 
Doglioni, C., & Cattoretti, G. (2013). Antibodies are forever: a study using 
12-26‐year‐old expired antibodies. Histopathology, 63(6), 869-876.
4. Drachenberg, C. B., Papadimitriou, J. C., Balaton, A. J., & Vaury, P. 
(2001). The total test approach to standardization of immunohistochemistry. 
Archives of pathology & laboratory medicine, 125(4), 471-471.


Regards

Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA)
Principal Scientist, the Children’s Hospital at Westmead
Adjunct Fellow, School of Medicine, University of Western Sydney
Tel: 612 9845 3306
Fax: 612 9845 3318
Pathology Department
the children's hospital at westmead
Cnr Hawkesbury Road and Hainsworth Street, Westmead
Locked Bag 4001, Westmead NSW 2145, AUSTRALIA



-Original Message-
From: jayalakshmy p.s via Histonet 

[Histonet] Pneumocystis Control Tissue

[Cartun, Richard via Histonet]

I apologize  someone contacted me several weeks ago about the availability 
of Pneumocystis control tissue, and I misplaced his name and contact 
information.  Please contact me again if you are still in need of control 
tissue.

Richard

Richard W. Cartun, MS, PhD
Director, Histology & The Martin M. Berman, MD
  Immunopathology/Morphologic Proteomics Laboratory
Assistant Director, Anatomic Pathology
Department of Pathology & Laboratory Medicine
Hartford Hospital
80 Seymour Street
Hartford, CT  06102
(860) 972-1596 Office
(860) 545-2204 (Fax)



This e-mail message, including any attachments, is for the sole use of the 
intended recipient(s) and may contain confidential and privileged information. 
Any unauthorized review, use, disclosure, or distribution is prohibited. If you 
are not the intended recipient, or an employee or agent responsible for 
delivering the message to the intended recipient, please contact the sender by 
reply e-mail and destroy all copies of the original message, including any 
attachments.
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

Re: [Histonet] Dr Freida Carson

[Cartun, Richard via Histonet]

I'm very sad to learn of her passing.  She was a remarkable person.  I didn't 
know her well, but I always enjoyed speaking with her at meetings and learning 
from her.  Many years ago I bought her entire collection of bound 
"Histotechnology" journals.

Richard

Richard W. Cartun, MS, PhD
Director, Histology & The Martin M. Berman, MD
  Immunopathology/Morphologic Proteomics Laboratory
Assistant Director, Anatomic Pathology
Department of Pathology & Laboratory Medicine
Hartford Hospital
80 Seymour Street
Hartford, CT  06102
(860) 972-1596 Office
(860) 545-2204 (Fax)




-Original Message-
From: Willis, Donna G via Histonet [mailto:histonet@lists.utsouthwestern.edu]
Sent: Wednesday, January 12, 2022 3:19 PM
To: Histonet (histonet@lists.utsouthwestern.edu)
Subject: [Histonet] Dr Freida Carson

EXTERNAL email from Outside HHC! Do NOT open attachments or click links from 
unknown senders.

For those of you that are not on Facebook you may not already know that 
yesterday Freida Carson, PhD became one of our Histology Angels.  I had the 
privilege of having her as my Histology Educator long before she wrote her 
first edition of Histotechnology.  But more precious to me than having her as 
my educator was that she was a friend.  She will be missed.  Thank you Freida 
for all that you have given to the Histology Profession and to me.  Rest In 
Peace singing in the Angels Choir.

Donna Willis
Anatomic Pathology Manager
Baylor Scott Health
Baylor University Medical Center
3500 Gaston Ave|Dallas, Texas 75246
214-820-2465 office|214-725-6184 mobile



**
The information contained in this e-mail may be privileged, confidential, 
and/or protected from disclosure. If you are the intended recipient, further 
disclosures are prohibited without proper authorization. If you are not the 
intended recipient (or have received this e-mail in error) please notify the 
sender immediately and destroy this e-mail. Any unauthorized copying, 
disclosure or distribution of the material in this e-mail is strictly 
prohibited and no waiver of any attorney-client, work product, or other 
privilege is intended. No binding agreement on behalf of Baylor Scott & White 
Health, or any affiliated entity, is permitted by e-mail without express 
written confirmation by a duly authorized representative of Baylor Scott & 
White Health.
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
https://urldefense.com/v3/__http://lists.utsouthwestern.edu/mailman/listinfo/histonet__;!!KCs9X-8!KrliN4WVvseLPZSwoK4W5cSk3P16SlHcjhT-eHXuJs7wbz1BM5BoX0Jty95frjfs5-E$


Reminder: This e-mail and any attachments are subject to the current HHC email 
retention policies. Please save or store appropriately in accordance with 
policy.

This e-mail message, including any attachments, is for the sole use of the 
intended recipient(s) and may contain confidential and privileged information. 
Any unauthorized review, use, disclosure, or distribution is prohibited. If you 
are not the intended recipient, or an employee or agent responsible for 
delivering the message to the intended recipient, please contact the sender by 
reply e-mail and destroy all copies of the original message, including any 
attachments.

___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

Re: [Histonet] Microwave slide drying

[Cartun, Richard via Histonet]

I have never used microwave drying for IHC slides.  I worry that if there is 
water trapped underneath the tissue section it might cause destruction of the 
tissue section if it is rapidly heated and "pops".  You could run a 
"side-by-side" comparison and see if you notice a difference.  Also, how would 
you monitor the temperature of the slide?  However, my philosophy has always 
been if it makes the process more efficient and/or less costly without changing 
the outcome, "do it".

Happy Thanksgiving to all who celebrate it.

Richard

Richard W. Cartun, MS, PhD
Director, Histology & The Martin M. Berman, MD
  Immunopathology/Morphologic Proteomics Laboratory
Assistant Director, Anatomic Pathology
Department of Pathology & Laboratory Medicine
Hartford Hospital
80 Seymour Street
Hartford, CT  06102
(860) 972-1596 Office
(860) 545-2204 (Fax)

-Original Message-
From: Samantha Golden via Histonet [mailto:histonet@lists.utsouthwestern.edu]
Sent: Tuesday, November 23, 2021 11:24 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Microwave slide drying

This email is from outside HHC. BE CAREFUL when opening attachments or links 
from unknown senders.

What are the Histonets thoughts toward drying your slides in the microwave….. 
pros/cons

I would specifically like feedback regarding the slides being used for IHC.

My new lab does this and it’s new to me.

Thanks!!
Samantha
Sent from my iPhone

___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
https://urldefense.com/v3/__http://lists.utsouthwestern.edu/mailman/listinfo/histonet__;!!KCs9X-8!IZrcGKqrTAB7YVkmihOC68-4NZ4y4YNfYD8Llt22Q6FJiPjxa6-C6OWEXWgdsjrijH8$


Reminder: This e-mail and any attachments are subject to the current HHC email 
retention policies. Please save or store appropriately in accordance with 
policy.

This e-mail message, including any attachments, is for the sole use of the 
intended recipient(s) and may contain confidential and privileged information. 
Any unauthorized review, use, disclosure, or distribution is prohibited. If you 
are not the intended recipient, or an employee or agent responsible for 
delivering the message to the intended recipient, please contact the sender by 
reply e-mail and destroy all copies of the original message, including any 
attachments.
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

Re: [Histonet] release of body parts

[Cartun, Richard via Histonet]

We no longer release any tissue to a patient that comes to our Pathology 
Laboratory in formalin, and our Legal Department supports this decision.  I 
know that our "Labor & Delivery" Unit has released placentas to patients; 
however, if the specimen doesn't come to Pathology, we don't get involved.  For 
certain types of specimens received in formalin (POC, fetus, amputation, etc.) 
a patient can request the release of their specimen, but they have to make 
arrangements with a funeral home or mortuary to take procession of the specimen 
here at the hospital and, yes, they (funeral home/mortuary) must sign the 
release form.

Please keep in mind that each state may have statues on the release of human 
tissue to patients or their families.  I also know that some towns here in CT 
have ordinances preventing residents from burying human tissue on their 
property.

Richard

Richard W. Cartun, MS, PhD
Director, Histology & The Martin M. Berman, MD
  Immunopathology/Morphologic Proteomics Laboratory
Assistant Director, Anatomic Pathology
Department of Pathology & Laboratory Medicine
Hartford Hospital
80 Seymour Street
Hartford, CT  06102
(860) 972-1596 Office
(860) 545-2204 (Fax)


-Original Message-
From: Nancy Schmitt via Histonet [mailto:histonet@lists.utsouthwestern.edu]
Sent: Wednesday, August 18, 2021 12:33 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] release of body parts

This email is from outside HHC. BE CAREFUL when opening attachments or links 
from unknown senders.

Hello-
We are seeing a bit more of patients that are requesting to take their body 
parts with them (uterus, POC, etc); I am talking home  - not the funeral home.
Are you using a release of body parts form to fill out with the patient?
Are you draining off the formalin, or sending in formalin with parafilm around 
the lid?
Thank you for your thoughts,
Nancy Schmitt MLT, HT(ASCP)
Pathology Support Services
Dubuque, IA  52001


Confidentiality Notice:
This e-mail, including any attachments is the property of Trinity Health and is 
intended for the sole use of the intended recipient(s). It may contain 
information that is privileged and confidential.  Any unauthorized review, use, 
disclosure, or distribution is prohibited. If you are not the intended 
recipient, please delete this message, and reply to the sender regarding the 
error in a separate email.
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
https://urldefense.com/v3/__http://lists.utsouthwestern.edu/mailman/listinfo/histonet__;!!KCs9X-8!Ls4flMgYVSY7YCD1T7Gjds4KhtEHb3BOoEcnwu4dOdSCDB0AuE9PAY4C6bzFO2P3djs$


Reminder: This e-mail and any attachments are subject to the current HHC email 
retention policies. Please save or store appropriately in accordance with 
policy.

This e-mail message, including any attachments, is for the sole use of the 
intended recipient(s) and may contain confidential and privileged information. 
Any unauthorized review, use, disclosure, or distribution is prohibited. If you 
are not the intended recipient, or an employee or agent responsible for 
delivering the message to the intended recipient, please contact the sender by 
reply e-mail and destroy all copies of the original message, including any 
attachments.

___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

Re: [Histonet] HSV1 and HSV2

[Cartun, Richard via Histonet]

Hi Martha:

We are using type-specific monoclonals from BioSB (Santa Barbara, CA).  They 
also sell a cocktail of the two antibodies which is what we use for screening.  
Rarely, do we get requests for type-specific identification.

Richard

Richard W. Cartun, MS, PhD
Director, Histology & The Martin M. Berman, MD Immunopathology/Morphologic 
Proteomics Laboratory
Director, Biospecimen Collection Programs
Assistant Director, Anatomic Pathology
Hartford Hospital
80 Seymour Street
Hartford, CT  06102
(860) 972-1596 (Office)
(860) 545-2204 (Fax)
richard.car...@hhchealth.org


-Original Message-
From: Martha Ward-Pathology via Histonet 
[mailto:histonet@lists.utsouthwestern.edu]
Sent: Tuesday, June 15, 2021 3:10 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] HSV1 and HSV2

CAUTION: This email is from outside HHC. USE CARE when opening attachments or 
links.

We have always offered these two antibodies separately but recently we have 
begun having issues with our HSV2.   In researching a new source I am seeing 
that some places do some sort of cocktail mixture of the two.   What are others 
out there doing for this?

Thanks in advance for your help.

Martha Ward, Manager
Molecular Diagnostics  Lab
Wake Forest Baptist Medical Center
Winston-Salem, NC 27157
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
https://urldefense.com/v3/__http://lists.utsouthwestern.edu/mailman/listinfo/histonet__;!!KCs9X-8!PM-BBy3bF7epr83ucPrDa0D21BtxagGkNt2mKWWY7SUaExNwbpL43czZRyw5F0A-LMQ$


Reminder: This e-mail and any attachments are subject to the current HHC email 
retention policies. Please save or store appropriately in accordance with 
policy.

This e-mail message, including any attachments, is for the sole use of the 
intended recipient(s) and may contain confidential and privileged information. 
Any unauthorized review, use, disclosure, or distribution is prohibited. If you 
are not the intended recipient, or an employee or agent responsible for 
delivering the message to the intended recipient, please contact the sender by 
reply e-mail and destroy all copies of the original message, including any 
attachments.

___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] Question - Bone marrow core biopy specimens

[Cartun, Richard via Histonet]

Hello everyone:

I'm wonder what your protocol is for cutting formalin-fixed, paraffin-embedded 
bone marrow core biopsy specimens?  Do you cut a limited number of slides 
upfront and then go back to the block if the pathologist requests histochemical 
stains or immunohistochemical tests?  We are implementing a protocol today 
where we will cut "15" slides upfront; 3 H at different levels and 12 
unstains for potential histochemical stains and/or IHC tests.  Are we crazy?

Thank you, and I hope everyone is doing well.

Richard

Richard W. Cartun, MS, PhD
Director, Histology & The Martin M. Berman, MD Immunopathology/Morphologic 
Proteomics Laboratory
Director, Biospecimen Collection Programs
Assistant Director, Anatomic Pathology
Hartford Hospital
80 Seymour Street
Hartford, CT  06102
(860) 972-1596 (Office)
(860) 545-2204 (Fax)
richard.car...@hhchealth.org


This e-mail message, including any attachments, is for the sole use of the 
intended recipient(s) and may contain confidential and privileged information. 
Any unauthorized review, use, disclosure, or distribution is prohibited. If you 
are not the intended recipient, or an employee or agent responsible for 
delivering the message to the intended recipient, please contact the sender by 
reply e-mail and destroy all copies of the original message, including any 
attachments.
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

Re: [Histonet] Antibody diluent

[Cartun, Richard via Histonet]

Interesting . I will definitely check this out.  I know I'm "Old School", 
but I have never "personalized" the diluent for preparing antibody dilutions.  
We have always used just one diluent for all the antibodies that we have run 
over my 43-year career.

Richard

Richard W. Cartun, MS, PhD
Director, Histology & The Martin M. Berman, MD Immunopathology/Morphologic 
Proteomics Laboratory
Director, Biospecimen Collection Programs
Assistant Director, Anatomic Pathology
Hartford Hospital
80 Seymour Street
Hartford, CT  06102
(860) 972-1596 (Office)
(860) 545-2204 (Fax)
richard.car...@hhchealth.org


-Original Message-
From: Sally Price via Histonet [mailto:histonet@lists.utsouthwestern.edu]
Sent: Wednesday, March 24, 2021 3:04 PM
To: histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Antibody diluent

STOP, THINK, READ. This is an external email. Exercise extra caution responding 
to it, opening attachments and following links.

Maria:
Personally, I believe in using the right tool for the job, and have learned
a lot about the science of IHC from Biocare.  I recently received an email
from them with a link to download a paper from their website that’s all
about diluents and I found this information very useful.  I would encourage
others to review this information if they want to learn why using a
universal reagent best option.
Sally--
Sally Price
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
https://urldefense.com/v3/__http://lists.utsouthwestern.edu/mailman/listinfo/histonet__;!!KCs9X-8!PTAypJybU6v0JAOUlrF0mUAU06D0FRdGfW0-KjTw1rTwgS0VrOBXsLT5ZZxcQUNtNNQ$


Reminder: This e-mail and any attachments are subject to the current HHC email 
retention policies. Please save or store appropriately in accordance with 
policy.

This e-mail message, including any attachments, is for the sole use of the 
intended recipient(s) and may contain confidential and privileged information. 
Any unauthorized review, use, disclosure, or distribution is prohibited. If you 
are not the intended recipient, or an employee or agent responsible for 
delivering the message to the intended recipient, please contact the sender by 
reply e-mail and destroy all copies of the original message, including any 
attachments.
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

Re: [Histonet] Controls

[Cartun, Richard via Histonet]

I would recommend waiting at least 2 weeks after the case is signed-out in case 
additional testing needs to be performed, especially if the specimen contains 
cancer.  And, I would always make sure that you leave representative tissue in 
the block meaning don't consume the entire specimen when you cut your control 
sections unless the case has multiple blocks showing the same pathology.

Richard

Richard W. Cartun, MS, PhD
Director, Histology & The Martin M. Berman, MD Immunopathology/Morphologic 
Proteomics Laboratory
Director, Biospecimen Collection Programs
Assistant Director, Anatomic Pathology
Hartford Hospital
80 Seymour Street
Hartford, CT  06102
(860) 972-1596 (Office)
(860) 545-2204 (Fax)
richard.car...@hhchealth.org

-Original Message-
From: Jacque Sagasser via Histonet [mailto:histonet@lists.utsouthwestern.edu]
Sent: Thursday, October 15, 2020 12:47 PM
To: 'Histonet (histonet@lists.utsouthwestern.edu)'
Subject: [Histonet] Controls

STOP, THINK, READ. This is an external email. Exercise extra caution responding 
to it, opening attachments and following links.

Good afternoon!

Could any of you tell me off hand how old patient tissue has to be before you 
can use it as a control? My lab has only been operating since February. We run 
quite a few Alcian Blue PAS stains a week, and my last control is getting 
sparse. I have some beautiful patient tissue that I could use if the time limit 
has been exceeded. I am trying to avoid purchasing controls because we are a 
small GI lab, and that eats into our operating costs. That said, if tissue has 
to be older than the amount of time our lab has been open, does anyone have a 
spare control they could pass on to me please? I would be willing to assist you 
with anything I am able to assist with in the future.

​Jacque R. Sagasser, HT (ASCP)cm
Gandhi GI Pathology, LLC
999 Brubaker Drive
Suite 1
Kettering OH 45429
jsagas...@gandhigi.com
937-795-1099 phone
937-519-1321 fax
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
https://urldefense.com/v3/__http://lists.utsouthwestern.edu/mailman/listinfo/histonet__;!!KCs9X-8!JE_DSMiJJOIqcoBfBHt09qvO0We4-zpHEADQfbNIr282bdD0Xju5hESmd9xhVlW2bMo$


Reminder: This e-mail and any attachments are subject to the current HHC email 
retention policies. Please save or store appropriately in accordance with 
policy.

This e-mail message, including any attachments, is for the sole use of the 
intended recipient(s) and may contain confidential and privileged information. 
Any unauthorized review, use, disclosure, or distribution is prohibited. If you 
are not the intended recipient, or an employee or agent responsible for 
delivering the message to the intended recipient, please contact the sender by 
reply e-mail and destroy all copies of the original message, including any 
attachments.
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] Formalin fixation for COVID-19 positive tissues .....

[Cartun, Richard via Histonet]

How long are you fixing surgical tissue specimens from COVID-19 positive 
patient's before tissue processing?  I know that the CDC is recommending "72" 
hours for autopsy tissues, but, to me, that seems excessive for surgical 
pathology specimens.  Any information that you can share on this subject would 
be appreciated.  Thank you and stay safe.

Richard

Richard W. Cartun, MS, PhD
Director, Histology & The Martin M. Berman, MD Immunopathology/Morphologic 
Proteomics Laboratory
Director, Biospecimen Collection Programs
Assistant Director, Anatomic Pathology
Hartford Hospital
80 Seymour Street
Hartford, CT  06102
(860) 972-1596 (Office)
(860) 545-2204 (Fax)
richard.car...@hhchealth.org


This e-mail message, including any attachments, is for the sole use of the 
intended recipient(s) and may contain confidential and privileged information. 
Any unauthorized review, use, disclosure, or distribution is prohibited. If you 
are not the intended recipient, or an employee or agent responsible for 
delivering the message to the intended recipient, please contact the sender by 
reply e-mail and destroy all copies of the original message, including any 
attachments.
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

Re: [Histonet] BRAF testing

[Cartun, Richard via Histonet]

This is a very difficult target to detect.  I was told recently by a vendor 
that one needs to use prolonged, very aggressive antigen retrieval (pressure 
cooker with high pH buffer) to get consistent results.  Also, the type of 
cancer may influence your success (colon vs. thyroid vs. melanoma).  I would be 
interested in knowing what others' experiences are.

Richard

Richard W. Cartun, MS, PhD
Director, Histology & The Martin M. Berman, MD Immunopathology/Morphologic 
Proteomics Laboratory Director, Biospecimen Collection Programs Assistant 
Director, Anatomic Pathology Hartford Hospital
80 Seymour Street
Hartford, CT  06102
(860) 972-1596 (Office)
(860) 545-2204 (Fax)
richard.car...@hhchealth.org

-Original Message-
From: Charles Riley via Histonet [mailto:histonet@lists.utsouthwestern.edu]
Sent: Friday, March 27, 2020 8:07 AM
To: Histo List
Subject: [Histonet] BRAF testing

This is an email from an external source. USE CAUTION opening attachments or 
links from unknown senders.

Can anyone send me a block that can be used to validate BRAF V600E  antibody.  
I have tried several cases that should work but our pathologists don't think it 
is working correctly. I just need a small core sample that is known to be 
positive. Any help would greatly be appreciated

--

Charles Riley BS  HT, HTL(ASCP)CM

Histopathology Coordinator/ Mohs
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
https://urldefense.com/v3/__http://lists.utsouthwestern.edu/mailman/listinfo/histonet__;!!KCs9X-8!Oq9k97gGgRatvdlIoqX-QkrcMRMS19Iuxle4pZY1FqKfIdEBOVk2YOJ3yVO0qAn3MBg$


Reminder: This e-mail and any attachments are subject to the current HHC email 
retention policies. Please save or store appropriately in accordance with 
policy.

This e-mail message, including any attachments, is for the sole use of the 
intended recipient(s) and may contain confidential and privileged information. 
Any unauthorized review, use, disclosure, or distribution is prohibited. If you 
are not the intended recipient, or an employee or agent responsible for 
delivering the message to the intended recipient, please contact the sender by 
reply e-mail and destroy all copies of the original message, including any 
attachments.

___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

Re: [Histonet] IHC troubleshooting

[Cartun, Richard via Histonet]

Are you using some form of antigen retrieval?  In addition to what has been 
discussed previously, antigen retrieval using heat (HIER) can cause tissue loss 
especially collagen.

Richard

Richard W. Cartun, MS, PhD
Director, Histology & The Martin M. Berman, MD Immunopathology/Morphologic 
Proteomics Laboratory
Director, Biospecimen Collection Programs
Assistant Director, Anatomic Pathology
Hartford Hospital
80 Seymour Street
Hartford, CT  06102
(860) 972-1596 (Office)
(860) 545-2204 (Fax)
richard.car...@hhchealth.org

-Original Message-
From: Charles Riley via Histonet [mailto:histonet@lists.utsouthwestern.edu]
Sent: Wednesday, February 26, 2020 9:44 AM
To: Histo List
Subject: [Histonet] IHC troubleshooting

EXTERNAL email from Outside HHC! Do NOT open attachments or click links from 
unknown senders.

Our pathologists are complaining that chunks of the dermis are missing from IHC 
slides yet the entire section is present prior to staining.

Does anyone have any ideas what could cause the tissue to not adhere to the
slides throughout the staining process? We use the Leica Bond stainers.

--

Charles Riley BS  HT, HTL(ASCP)CM

Histopathology Coordinator/ Mohs
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthwestern.edu_mailman_listinfo_histonet=DwICAg=e_HtEeZEQXP5NUOb33qoTj0AVvRFBS9_rhBTQcfkWoA=eCMS5E4UqfGKTlPknIMjMlCkk-KGKHflslRsFz3l5JE=DSmBsjGihQbMxzBLaqCbr8whSAZVh5SjOAr5sVbspE8=Bs1CxoLt5gMxm60Er8yfxoTB45uLgwau1GA_3MK5-jQ=


Reminder: This e-mail and any attachments are subject to the current HHC email 
retention policies. Please save or store appropriately in accordance with 
policy.

This e-mail message, including any attachments, is for the sole use of the 
intended recipient(s) and may contain confidential and privileged information. 
Any unauthorized review, use, disclosure, or distribution is prohibited. If you 
are not the intended recipient, or an employee or agent responsible for 
delivering the message to the intended recipient, please contact the sender by 
reply e-mail and destroy all copies of the original message, including any 
attachments.

___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] Cellient for cell block preparation

[Cartun, Richard via Histonet]

Question - What has been your experience with  Hologic's Cellient instrument 
for preparing cell block specimens?  I understand that it is very good for 
pauci-cellular specimens.  However, I am concerned about using these 
preparations for immunohistochemical and DNA/RNA testing.  Thank you!

Richard

Richard W. Cartun, MS, PhD
Director, Histology & The Martin M. Berman, MD Immunopathology/Morphologic 
Proteomics Laboratory
Director, Biospecimen Collection Programs
Assistant Director, Anatomic Pathology
Hartford Hospital
80 Seymour Street
Hartford, CT  06102
(860) 972-1596 (Office)
(860) 545-2204 (Fax)
richard.car...@hhchealth.org


This e-mail message, including any attachments, is for the sole use of the 
intended recipient(s) and may contain confidential and privileged information. 
Any unauthorized review, use, disclosure, or distribution is prohibited. If you 
are not the intended recipient, or an employee or agent responsible for 
delivering the message to the intended recipient, please contact the sender by 
reply e-mail and destroy all copies of the original message, including any 
attachments.
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

Re: [Histonet] PLAG1

[Cartun, Richard via Histonet]

Looking at an article in Histopathology (January 2018) on PLAG1 IHC testing, 
the authors used clone "3B7" (Novus Biologicals in Littleton, CO) on the Bond 
Max IHC platform form.

Richard

Richard W. Cartun, MS, PhD
Director, Histology & The Martin M. Berman, MD Immunopathology/Morphologic 
Proteomics Laboratory
Director, Biospecimen Collection Programs
Assistant Director, Anatomic Pathology
Hartford Hospital
80 Seymour Street
Hartford, CT  06102
(860) 972-1596 (Office)
(860) 545-2204 (Fax)
richard.car...@hhchealth.org


-Original Message-
From: Charles Riley via Histonet [mailto:histonet@lists.utsouthwestern.edu]
Sent: Monday, October 28, 2019 10:20 AM
To: Histo List
Subject: [Histonet] PLAG1

EXTERNAL email is from outside HHC. DO NOT open attachments or click links from 
unknown senders.

My pathologists want to bring this test in house. Can anyone suggest a

company I can purchase this antibody for testing via IHC on the Leica Bond

platform?



--



Charles Riley BS  HT, HTL(ASCP)CM



Histopathology Coordinator/ Mohs

___

Histonet mailing list

Histonet@lists.utsouthwestern.edu

https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthwestern.edu_mailman_listinfo_histonet=DwICAg=e_HtEeZEQXP5NUOb33qoTj0AVvRFBS9_rhBTQcfkWoA=eCMS5E4UqfGKTlPknIMjMlCkk-KGKHflslRsFz3l5JE=4VoB34evfULROSYTZirfyVjGhHd9khhhTv1g4pUQmxY=jqmKMH0dL56TvREZ4Edi5Oza2RVUTXB-PoqLdT5Sc2k=



Reminder: This e-mail and any attachments are subject to the current HHC email 
retention policies. Please save or store appropriately in accordance with 
policy.

This e-mail message, including any attachments, is for the sole use of the 
intended recipient(s) and may contain confidential and privileged information. 
Any unauthorized review, use, disclosure, or distribution is prohibited. If you 
are not the intended recipient, or an employee or agent responsible for 
delivering the message to the intended recipient, please contact the sender by 
reply e-mail and destroy all copies of the original message, including any 
attachments.

___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

Re: [Histonet] Cell block processing

[Cartun, Richard via Histonet]

I agree with Joe.  We used to use ETOH for cell blocks, but stopped using it 
when we started doing IHC biomarker testing on these specimens.  Alcohol is 
good for some proteomic targets, but can be a disaster for others.  We also fix 
all of our cell block specimens that are collected in saline or RPMI in 
formalin.

Richard

Richard W. Cartun, MS, PhD
Director, Histology & The Martin M. Berman, MD Immunopathology/Morphologic 
Proteomics Laboratory
Director, Biospecimen Collection Programs
Assistant Director, Anatomic Pathology
Hartford Hospital
80 Seymour Street
Hartford, CT  06102
(860) 972-1596 (Office)
(860) 545-2204 (Fax)
richard.car...@hhchealth.org

-Original Message-
From: Joe W. Walker, Jr. via Histonet [mailto:histonet@lists.utsouthwestern.edu]
Sent: Friday, October 25, 2019 4:36 PM
To: Charles Riley
Cc: histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Cell block processing

EXTERNAL email is from outside HHC. DO NOT open attachments or click links from 
unknown senders.

As a cytotech, that wouldn’t be my first choice for collections and FNA 
specimens.  The main reason is that once fixed in 95% ETOH you are limited if 
you need to perform IHC stains on the cell block unless you have validated your 
IHCs on ETOH fixed specimens.  How do you process the FNA rinses that in in 
ETOH: Only Cell blocks or do you have another cytology liquid prep?



Without knowing your prep process, I’d suggest collecting the FNA needle rinses 
in Hank’s Balanced Salt solution.  After making the cell block, you could then 
formalin fix them.  I can send you a procedure that we utilize for this 
process.  The cell blocks cut great, look great, and you can perform IHC an 
molecular testing if needed.



Joe Walker



From: Charles Riley 

Sent: Friday, October 25, 2019 12:57 PM

To: Joe W. Walker, Jr. 

Cc: histonet@lists.utsouthwestern.edu

Subject: Re: [Histonet] Cell block processing



[External Email] This email originated from outside of the organization. Think 
before you click: Don’t click on links, open attachments or respond to requests 
for sensitive information if the email looks suspicious or you don’t recognize 
the sender.



Our tech said they use 95% alcohol to collect the specimen.



On Fri, Oct 25, 2019 at 12:23 PM Joe W. Walker, Jr. 
mailto:jwwal...@rrmc.org>> wrote:

Hi Charles,



What are you collecting the FNA into?  Cytorich? Cytolyt? Other?



Joe W. Walker, Jr. MS, SCT(ASCP)

Anatomical Pathology Manager

joewal...@rrmc.org, 
https://urldefense.proofpoint.com/v2/url?u=http-3A__www.rrmc.org=DwIGaQ=e_HtEeZEQXP5NUOb33qoTj0AVvRFBS9_rhBTQcfkWoA=eCMS5E4UqfGKTlPknIMjMlCkk-KGKHflslRsFz3l5JE=2CdTK8PmO1-CXFcs6R-WWhnxgbCm1AHZ9wJ_1YOqDoA=JIavq_Wfz4S4TppCsMv8kQfnmD9wLQhRVNqSm1eJAO4=
 




-Original Message-

From: Charles Riley via Histonet 
mailto:histonet@lists.utsouthwestern.edu>>

Sent: Friday, October 25, 2019 8:13 AM

To: Histo List 
mailto:histonet@lists.utsouthwestern.edu>>

Subject: [Histonet] Cell block processing



[External Email] This email originated from outside of the organization. Think 
before you click: Don’t click on links, open attachments or respond to requests 
for sensitive information if the email looks suspicious or you don’t recognize 
the sender.





Does anyone have any tips or suggestions on how to better process extremely

bloody FNA  specimens?Is there anyway to clear out some or all of the

blood without destroying the other tissues?



--



Charles Riley BS  HT, HTL(ASCP)CM



Histopathology Coordinator/ Mohs

___

Histonet mailing list

Histonet@lists.utsouthwestern.edu

Re: [Histonet] Chromogenic ACD RNA ISH probes

[Cartun, Richard via Histonet]

We are currently running RNAScope for HPV and Albumin on our Leica Bond IIIs.  
It's a wonderful technology!

Richard

Richard W. Cartun, MS, PhD
Director, Histology & The Martin M. Berman, MD Immunopathology/Morphologic 
Proteomics Laboratory
Director, Biospecimen Collection Programs
Assistant Director, Anatomic Pathology
Hartford Hospital
80 Seymour Street
Hartford, CT  06102
(860) 972-1596 (Office)
(860) 545-2204 (Fax)
richard.car...@hhchealth.org




-Original Message-
From: Paula via Histonet [mailto:histonet@lists.utsouthwestern.edu]
Sent: Thursday, September 12, 2019 10:45 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Chromogenic ACD RNA ISH probes

THIS email is from Outside HHC! BE CAREFUL when opening attachments or click 
links from unknown senders.

Hello everyone,







We recently received informative handouts regarding this technology from our

Leica rep and we are in the process of scheduling a meeting to learn more

about it. They say it's so wonderful, our jaws will drop from how beautiful

the stains are.







Can anyone comment on this testing? Would it be a nice addition to our

library of IHC tests?







Any comments are welcomed!







Thank you in advance,







Paula



Lab Manager for Bio-Path Medical Group



___

Histonet mailing list

Histonet@lists.utsouthwestern.edu

https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthwestern.edu_mailman_listinfo_histonet=DwICAg=e_HtEeZEQXP5NUOb33qoTj0AVvRFBS9_rhBTQcfkWoA=eCMS5E4UqfGKTlPknIMjMlCkk-KGKHflslRsFz3l5JE=E-WYJcMx9jdIa0p_q-5zjdtbDEqRoQJzZoEbVbzdGbc=VsAW0b6kdEIk2R5Hk67LryzbvHNN9iPdxFz8lAbum_I=



Reminder: This e-mail and any attachments are subject to the current HHC email 
retention policies. Please save or store appropriately in accordance with 
policy.

This e-mail message, including any attachments, is for the sole use of the 
intended recipient(s) and may contain confidential and privileged information. 
Any unauthorized review, use, disclosure, or distribution is prohibited. If you 
are not the intended recipient, or an employee or agent responsible for 
delivering the message to the intended recipient, please contact the sender by 
reply e-mail and destroy all copies of the original message, including any 
attachments.

___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] Tissue Contamination

[Cartun, Richard via Histonet]

What are people doing to ensure that there is no tissue carry-over on 
instruments between cases when grossing?  Thank you.

Richard

Richard W. Cartun, MS, PhD
Director, Histology & The Martin M. Berman, MD Immunopathology/Morphologic 
Proteomics Laboratory
Director, Biospecimen Collection Programs
Assistant Director, Anatomic Pathology
Hartford Hospital
80 Seymour Street
Hartford, CT  06102
(860) 972-1596 (Office)
(860) 545-2204 (Fax)
richard.car...@hhchealth.org


This e-mail message, including any attachments, is for the sole use of the 
intended recipient(s) and may contain confidential and privileged information. 
Any unauthorized review, use, disclosure, or distribution is prohibited. If you 
are not the intended recipient, or an employee or agent responsible for 
delivering the message to the intended recipient, please contact the sender by 
reply e-mail and destroy all copies of the original message, including any 
attachments.
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] Major skin resections - CPT coding

[Cartun, Richard via Histonet]

I'm curious; how do you code major (complex) skin resections that also include 
soft tissue?  These cases often have 10-30 paraffin blocks and lots of H 
slides.  I'm thinking that they could be coded 88307 (simple resection) or 
88309 (complex resection).  I found out that our Dermatopathologist was coding 
these as "88305" and I don't think that is appropriate.  Thank you in advance 
for your comments.

Richard

Richard W. Cartun, MS, PhD
Director, Histology & The Martin M. Berman, MD Immunopathology/Morphologic 
Proteomics Laboratory
Director, Biospecimen Collection Programs
Assistant Director, Anatomic Pathology
Hartford Hospital
80 Seymour Street
Hartford, CT  06102
(860) 972-1596 (Office)
(860) 545-2204 (Fax)
richard.car...@hhchealth.org


This e-mail message, including any attachments, is for the sole use of the 
intended recipient(s) and may contain confidential and privileged information. 
Any unauthorized review, use, disclosure, or distribution is prohibited. If you 
are not the intended recipient, or an employee or agent responsible for 
delivering the message to the intended recipient, please contact the sender by 
reply e-mail and destroy all copies of the original message, including any 
attachments.
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

Re: [Histonet] [External] Re: SDHB IVD antibody

[Cartun, Richard via Histonet]

We use the antibody at a dilution of 1:50 following low-pH HIER (20 minutes) on 
the Leica Bond Max IHC platform.  Our primary antibody incubation is 15 
minutes.  Detection is Bond Refine HRP with DAB. 

Richard

Richard W. Cartun, MS, PhD
Director, Histology & The Martin M. Berman, MD Immunopathology/Morphologic 
Proteomics Laboratory
Director, Biospecimen Collection Programs
Assistant Director, Anatomic Pathology
Hartford Hospital
80 Seymour Street
Hartford, CT  06102
(860) 972-1596 (Office)
(860) 545-2204 (Fax)
richard.car...@hhchealth.org

-Original Message-
From: Bitting, Angela K. [mailto:akbitt...@geisinger.edu] 
Sent: Tuesday, June 25, 2019 1:03 PM
To: Cartun, Richard
Subject: RE: [External] Re: [Histonet] SDHB IVD antibody

EXTERNAL email from Outside HHC! Do NOT open attachments or click links from 
unknown senders.

Would you share your procedure?


-Original Message-
From: Cartun, Richard via Histonet [mailto:histonet@lists.utsouthwestern.edu]
Sent: Tuesday, June 25, 2019 12:31 PM
To: Emmanuel Geoffrey S Gonzaga
Cc: histonet@lists.utsouthwestern.edu
Subject: [External] Re: [Histonet] SDHB IVD antibody

Cell Marque offers an excellent SDHB rabbit monoclonal (clone EP288) that we 
use in our laboratory.  However, it is not an "IVD".

Richard

Richard W. Cartun, MS, PhD
Director, Histology & The Martin M. Berman, MD Immunopathology/Morphologic 
Proteomics Laboratory Director, Biospecimen Collection Programs Assistant 
Director, Anatomic Pathology Hartford Hospital
80 Seymour Street
Hartford, CT  06102
(860) 972-1596 (Office)
(860) 545-2204 (Fax)
richard.car...@hhchealth.org


-Original Message-
From: Emmanuel Geoffrey S Gonzaga via Histonet 
[mailto:histonet@lists.utsouthwestern.edu]
Sent: Tuesday, June 25, 2019 11:45 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] SDHB IVD antibody

EXTERNAL email from Outside HHC! Do NOT open attachments or click links from 
unknown senders.

Hello Histonet!



Does anyone know of a vendor that offers SDHB IVD antibody? It looks like Zeta 
corp. use to offer it, but has since discontinued production.



Thanks



Emmanuel Gonzaga

NOTICE TO RECIPIENT:  If you are not the intended recipient of this e-mail, you 
are prohibited from sharing, copying, or otherwise using or disclosing its 
contents.  If you have received this e-mail in error, please notify the sender 
immediately by reply e-mail and permanently delete this e-mail and any 
attachments without reading, forwarding or saving them.  Thank you.

___

Histonet mailing list

Histonet@lists.utsouthwestern.edu

https://urldefense.proofpoint.com/v2/url?u=https-3A__nam05.safelinks.protection.outlook.com_-3Furl-3Dhttp-253A-252F-252Flists.utsouthwestern.edu-252Fmailman-252Flistinfo-252Fhistonet-26amp-3Bdata-3D02-257C01-257Cakbitting-2540geisinger.edu-257C3b068d408057499ffb5b08d6f98a8191-257C37d46c567c664402a16055c2313b910d-257C0-257C0-257C636970770609939249-26amp-3Bsdata-3DdmRWqzjYQ-252FklAuI8b2SveBJ0TZeJwP9To0hqSQNsSZ8-253D-26amp-3Breserved-3D0-3D=DwIFAg=e_HtEeZEQXP5NUOb33qoTj0AVvRFBS9_rhBTQcfkWoA=eCMS5E4UqfGKTlPknIMjMlCkk-KGKHflslRsFz3l5JE=kDrLmbGHFDql7aeIYFDOs1iXhto-2xJwm-YHB3kQrlc=FaYYo3pr3pU9uI75QtH3yMu4ajvZ1ueL8_FwBz_KVC4=



Reminder: This e-mail and any attachments are subject to the current HHC email 
retention policies. Please save or store appropriately in accordance with 
policy.

This e-mail message, including any attachments, is for the sole use of the 
intended recipient(s) and may contain confidential and privileged information. 
Any unauthorized review, use, disclosure, or distribution is prohibited. If you 
are not the intended recipient, or an employee or agent responsible for 
delivering the message to the intended recipient, please contact the sender by 
reply e-mail and destroy all copies of the original message, including any 
attachments.

___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
https://urldefense.proofpoint.com/v2/url?u=https-3A__nam05.safelinks.protection.outlook.com_-3Furl-3Dhttp-253A-252F-252Flists.utsouthwestern.edu-252Fmailman-252Flistinfo-252Fhistonet-26amp-3Bdata-3D02-257C01-257Cakbitting-2540geisinger.edu-257C3b068d408057499ffb5b08d6f98a8191-257C37d46c567c664402a16055c2313b910d-257C0-257C0-257C636970770609939249-26amp-3Bsdata-3DdmRWqzjYQ-252FklAuI8b2SveBJ0TZeJwP9To0hqSQNsSZ8-253D-26amp-3Breserved-3D0=DwIFAg=e_HtEeZEQXP5NUOb33qoTj0AVvRFBS9_rhBTQcfkWoA=eCMS5E4UqfGKTlPknIMjMlCkk-KGKHflslRsFz3l5JE=kDrLmbGHFDql7aeIYFDOs1iXhto-2xJwm-YHB3kQrlc=mY-_buKq3CWkMvt4PkoT4yNkgfK5I77byNyk_Ak37zo=


IMPORTANT WARNING: The information in this message (and the documents attached 
to it, if any) is confidential and may be legally privileged. It is intended 
solely for the addressee. Access to this message by anyone else is 
unauthorized. If you are not the intended recipient, any disclosure, copying, 
distribution or any action taken, or o

Re: [Histonet] SDHB IVD antibody

[Cartun, Richard via Histonet]

Cell Marque offers an excellent SDHB rabbit monoclonal (clone EP288) that we 
use in our laboratory.  However, it is not an "IVD".

Richard

Richard W. Cartun, MS, PhD
Director, Histology & The Martin M. Berman, MD Immunopathology/Morphologic 
Proteomics Laboratory
Director, Biospecimen Collection Programs
Assistant Director, Anatomic Pathology
Hartford Hospital
80 Seymour Street
Hartford, CT  06102
(860) 972-1596 (Office)
(860) 545-2204 (Fax)
richard.car...@hhchealth.org


-Original Message-
From: Emmanuel Geoffrey S Gonzaga via Histonet 
[mailto:histonet@lists.utsouthwestern.edu]
Sent: Tuesday, June 25, 2019 11:45 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] SDHB IVD antibody

EXTERNAL email from Outside HHC! Do NOT open attachments or click links from 
unknown senders.

Hello Histonet!



Does anyone know of a vendor that offers SDHB IVD antibody? It looks like Zeta 
corp. use to offer it, but has since discontinued production.



Thanks



Emmanuel Gonzaga

NOTICE TO RECIPIENT:  If you are not the intended recipient of this e-mail, you 
are prohibited from sharing, copying, or otherwise using or disclosing its 
contents.  If you have received this e-mail in error, please notify the sender 
immediately by reply e-mail and permanently delete this e-mail and any 
attachments without reading, forwarding or saving them.  Thank you.

___

Histonet mailing list

Histonet@lists.utsouthwestern.edu

https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthwestern.edu_mailman_listinfo_histonet=DwICAg=e_HtEeZEQXP5NUOb33qoTj0AVvRFBS9_rhBTQcfkWoA=eCMS5E4UqfGKTlPknIMjMlCkk-KGKHflslRsFz3l5JE=sXVK0Xe8QReHqo7qgu1MJb4LNcEWvtOhWJtZqTlz6VU=00oYxFdFjiS9Xv35cO6xYzVUKYzL7kHq8-rQlhgkxIE=



Reminder: This e-mail and any attachments are subject to the current HHC email 
retention policies. Please save or store appropriately in accordance with 
policy.

This e-mail message, including any attachments, is for the sole use of the 
intended recipient(s) and may contain confidential and privileged information. 
Any unauthorized review, use, disclosure, or distribution is prohibited. If you 
are not the intended recipient, or an employee or agent responsible for 
delivering the message to the intended recipient, please contact the sender by 
reply e-mail and destroy all copies of the original message, including any 
attachments.

___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] H Artifact

[Cartun, Richard via Histonet]

We had several cases last Friday that showed "optically clear nuclei" 
(primarily lymphocytes) on H  Have you seen this artifact and, if so, what 
is it due do?  I will be happy to e-mail a photo to anyone interested.  Thank 
you as always for your help.

Richard

Richard W. Cartun, MS, PhD
Director, Histology & The Martin M. Berman, MD Immunopathology/Morphologic 
Proteomics Laboratory
Director, Biospecimen Collection Programs
Assistant Director, Anatomic Pathology
Hartford Hospital
80 Seymour Street
Hartford, CT  06102
(860) 972-1596 (Office)
(860) 545-2204 (Fax)
richard.car...@hhchealth.org


This e-mail message, including any attachments, is for the sole use of the 
intended recipient(s) and may contain confidential and privileged information. 
Any unauthorized review, use, disclosure, or distribution is prohibited. If you 
are not the intended recipient, or an employee or agent responsible for 
delivering the message to the intended recipient, please contact the sender by 
reply e-mail and destroy all copies of the original message, including any 
attachments.
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

Re: [Histonet] ER/PR controls

[Cartun, Richard via Histonet]

We use internal control blocks that we prepare from extra tumor tissue that we 
process after the case has been signed out.  However, this is getting more and 
more difficult for us since most breast tumors are smaller now when detected 
(and that's a good thing!) which results in the entire tumor being submitted 
for diagnosis.   I like to select a control case where we have multiple blocks 
(>10), and where the tumor shows variable (weak, moderate, and strong) 
immunoreactivity for both ER and PR.  It's also beneficial when your control 
tissue contains benign breast epithelium which can serve as both a positive and 
negative control.  In my experience, most breast biopsy specimens show internal 
positive controls for ER and PR which is especially helpful when the tumor 
shows a "Triple-Negative" immunoprofile.  After all, the best positive control 
is an internal positive control!  When I look at my positive controls on a 
daily basis, I am looking for reproducibility in the immunoreactivity in the 
tumor and the benign epithelium.

Richard

Richard W. Cartun, MS, PhD
Director, Histology & The Martin M. Berman, MD Immunopathology/Morphologic 
Proteomics Laboratory
Director, Biospecimen Collection Programs
Assistant Director, Anatomic Pathology
Hartford Hospital
80 Seymour Street
Hartford, CT  06102
(860) 972-1596 (Office)
(860) 545-2204 (Fax)
richard.car...@hhchealth.org

-Original Message-
From: Moe, Barbi A via Histonet [mailto:histonet@lists.utsouthwestern.edu]
Sent: Wednesday, March 13, 2019 1:44 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] ER/PR controls

EXTERNAL email from Outside HHC! Do NOT open attachments or click links from 
unknown senders.

For anyone making in-house ER/PR controls could you comment on what tissue you 
have in your control block -- one piece of positive tumor regardless of 
reactivity strength 1+, 2+, 3+ (weak, medium, strong) or multiple pieces to 
represent each level of reactivity?





Also, does anyone order commercially their ER/PR controls to achieve this level 
of representation in their control block?





Any thoughts are appreciated - thank you.





Barb Moe



Gundersen Health System



La Crosse WI



ba...@gundersenhealth.org

___

Histonet mailing list

Histonet@lists.utsouthwestern.edu

https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthwestern.edu_mailman_listinfo_histonet=DwICAg=e_HtEeZEQXP5NUOb33qoTj0AVvRFBS9_rhBTQcfkWoA=eCMS5E4UqfGKTlPknIMjMlCkk-KGKHflslRsFz3l5JE=Y9uEoty_f-4ICuNypilMcFFogkMgeRwkinFP21id2IE=ZE2AOf2Q664rWxQL1OQKmR6u6WuZ8xEv0orVhTW8NJw=



Reminder: This e-mail and any attachments are subject to the current HHC email 
retention policies. Please save or store appropriately in accordance with 
policy.

This e-mail message, including any attachments, is for the sole use of the 
intended recipient(s) and may contain confidential and privileged information. 
Any unauthorized review, use, disclosure, or distribution is prohibited. If you 
are not the intended recipient, or an employee or agent responsible for 
delivering the message to the intended recipient, please contact the sender by 
reply e-mail and destroy all copies of the original message, including any 
attachments.

___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] Ventana p16 antibody

[Cartun, Richard via Histonet]

We use the Roche/Ventana p16 antibody (clone E6H4(tm)) on the Leica Bond Max.  
We are having a difficult time trying to order the antibody.  Can someone 
verify the catalog number (705-4713) for me?  Thank you.

Richard

Richard W. Cartun, MS, PhD
Director, Histology & The Martin M. Berman, MD Immunopathology/Morphologic 
Proteomics Laboratory
Director, Biospecimen Collection Programs
Assistant Director, Anatomic Pathology
Hartford Hospital
80 Seymour Street
Hartford, CT  06102
(860) 972-1596 (Office)
(860) 545-2204 (Fax)
richard.car...@hhchealth.org


This e-mail message, including any attachments, is for the sole use of the 
intended recipient(s) and may contain confidential and privileged information. 
Any unauthorized review, use, disclosure, or distribution is prohibited. If you 
are not the intended recipient, or an employee or agent responsible for 
delivering the message to the intended recipient, please contact the sender by 
reply e-mail and destroy all copies of the original message, including any 
attachments.
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] Question for Leica Bond IHC Users

[Cartun, Richard via Histonet]

We are preparing to install a Leica "Bond Stain Interface" which will allow us 
to move away from having to print slide labels manually for our IHC slides.  I 
am not an expert on this so I am looking for help.  We have "5" Bond IHC 
instruments that will be hooked-up to the controller; however, we have been 
told that we cannot connect our 6th instrument unless we get a second 
controller (and that's not going to happen).  Has anyone else been in a similar 
predicament?  And, if so, how have you resolved it?  We need to use all "6" 
instruments.  Thank you.

Richard

Richard W. Cartun, MS, PhD
Director, Histology & The Martin M. Berman, MD Immunopathology/Morphologic 
Proteomics Laboratory
Director, Biospecimen Collection Programs
Assistant Director, Anatomic Pathology
Hartford Hospital
80 Seymour Street
Hartford, CT  06102
(860) 972-1596 (Office)
(860) 545-2204 (Fax)
richard.car...@hhchealth.org


This e-mail message, including any attachments, is for the sole use of the 
intended recipient(s) and may contain confidential and privileged information. 
Any unauthorized review, use, disclosure, or distribution is prohibited. If you 
are not the intended recipient, or an employee or agent responsible for 
delivering the message to the intended recipient, please contact the sender by 
reply e-mail and destroy all copies of the original message, including any 
attachments.
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

Re: [Histonet] Derm IHC question

[Cartun, Richard via Histonet]

Doesn't sound like a fixation issue to me.  Could the tissue be drying out 
before it's placed in formalin?  Also, are these specimens inked for assessment 
of margins?  I've seen ink interfere with immunoreactivity.

Richard

Richard W. Cartun, MS, PhD
Director, Histology & The Martin M. Berman, MD Immunopathology/Morphologic 
Proteomics Laboratory
Director, Biospecimen Collection Programs
Assistant Director, Anatomic Pathology
Hartford Hospital
80 Seymour Street
Hartford, CT  06102
(860) 972-1596 (Office)
(860) 545-2204 (Fax)
richard.car...@hhchealth.org

-Original Message-
From: Debra Siena via Histonet [mailto:histonet@lists.utsouthwestern.edu]
Sent: Friday, February 08, 2019 11:51 AM
To: 'histonet'
Subject: [Histonet] Derm IHC question

CAUTION: This email is from outside HHC. USE CARE when opening attachments or 
links.

Hello fellow Histonetters

I would like to ask you a question about IHC staining and derm cases.  I am 
seeing a peculiar issue going on, where the melanocytes in the middle of the 
tissues are staining pretty well but when you get to the ends of the tissues 
either shaves or ellipses, they are not staining. This is sporadic, not every 
case and there is no consensus as to a common thread between the cases.I 
feel that this may be a fixation issue but was just wondering if anyone had 
ever seen the same phenomena and would be willing to share the theory or even 
better what was the remedy behind this issue.  The fixative is 10% Neutral 
Buffered Formaliln  and the cells in question that are "dropping out" which is 
what the pathologist is describing are melanocytes, especially with Sox-10 and 
Mart 1 antibodies.

Thanks for the assistance, I definitely appreciate it very much.



Best wishes,



[image001]  Debbie Siena, HT(ASCP)QIHC

Empowering Anatomic Pathology

Technical Support Manager, StatLab

2090 Commerce| McKinney, TX 75069

t: 800.442.3573 ext 229 | m: 469-400-6897 | f: 972-436-1369

dsi...@statlab.com|https://urldefense.proofpoint.com/v2/url?u=http-3A__www.statlab.com=DwICAg=e_HtEeZEQXP5NUOb33qoTj0AVvRFBS9_rhBTQcfkWoA=eCMS5E4UqfGKTlPknIMjMlCkk-KGKHflslRsFz3l5JE=niLAvgTbNiAqFJIM-oaLdmyfDgppcOQotF3-vo_Qv1M=y7Dkipiv1JdLkqba8XolhtugffjOReKOTWuW0mZaJJI=



StatLab is an ISO 13485 Certified Company



___

Histonet mailing list

Histonet@lists.utsouthwestern.edu

https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthwestern.edu_mailman_listinfo_histonet=DwICAg=e_HtEeZEQXP5NUOb33qoTj0AVvRFBS9_rhBTQcfkWoA=eCMS5E4UqfGKTlPknIMjMlCkk-KGKHflslRsFz3l5JE=niLAvgTbNiAqFJIM-oaLdmyfDgppcOQotF3-vo_Qv1M=9GfWQBzHH2U1LIbnBXO5wvUeCTlJulcFAkXN5aWrbCw=



Reminder: This e-mail and any attachments are subject to the current HHC email 
retention policies. Please save or store appropriately in accordance with 
policy.

This e-mail message, including any attachments, is for the sole use of the 
intended recipient(s) and may contain confidential and privileged information. 
Any unauthorized review, use, disclosure, or distribution is prohibited. If you 
are not the intended recipient, or an employee or agent responsible for 
delivering the message to the intended recipient, please contact the sender by 
reply e-mail and destroy all copies of the original message, including any 
attachments.

___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

Re: [Histonet] ER/PR question

[Cartun, Richard via Histonet]

If the tumor is ER/PR negative the first thing I do is to look for an internal 
positive control (immunoreactive benign breast epithelium).  In my experience, 
the majority of these cases have internal positive controls to validate the 
negative ER/PR results.  When I don't see internal positive controls, and the 
HER2 is negative, I recommend that the testing be repeated on excisional tumor 
for confirmation.  This is why it's important to submit adjacent benign breast 
tissue along with the tumor tissue when grossing.

Richard

Richard W. Cartun, MS, PhD
Director, Histology & The Martin M. Berman, MD Immunopathology/Morphologic 
Proteomics Laboratory
Director, Biospecimen Collection Programs
Assistant Director, Anatomic Pathology
Hartford Hospital
80 Seymour Street
Hartford, CT  06102
(860) 972-1596 (Office)
(860) 545-2204 (Fax)
richard.car...@hhchealth.org

-Original Message-
From: Greg Dobbin via Histonet [mailto:histonet@lists.utsouthwestern.edu]
Sent: Friday, February 08, 2019 8:52 AM
To: karen.heckf...@dignityhealth.org; histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] ER/PR question

CAUTION: This email is from outside HHC. USE CARE when opening attachments or 
links.

Hi Karen,

As mentioned by others "decay" is not likely going to be an issue. More

concerning for you could be not knowing how those tissues were handled

prior to processing 10 years ago.



Presumably, you now track cold ischemic times and have standardized your

fixation protocols for breast tissues and you have validated your IHC

procedures with these current parameters. If your lab was like many others

10 years ago, these parameters were probably not maintained so rigorously.

So interpretation of a negative result may be problematic.



Having said that, there is the internal control for both ER and PR. It

seems to me that if the internal control is staining adequately, then

perhaps interpretation will not be an issue. I am curious, does anyone have

a different take on relying the internal control as a guide in this

particular situation??

Greg



--

*Greg Dobbin*

1205 Pleasant Grove Rd

RR#2 York,

PE  C0A 1P0





*Everything in moderation...even moderation itself**!*

___

Histonet mailing list

Histonet@lists.utsouthwestern.edu

https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthwestern.edu_mailman_listinfo_histonet=DwICAg=e_HtEeZEQXP5NUOb33qoTj0AVvRFBS9_rhBTQcfkWoA=eCMS5E4UqfGKTlPknIMjMlCkk-KGKHflslRsFz3l5JE=6t6lv-HaXrWz9L26Ub8EE5c9k76MJqlTDQ1XNA9cb2Q=_Gu4paw-d2iygUvP770iXrMhJFfeQlU9bt3MZLe-NIA=



Reminder: This e-mail and any attachments are subject to the current HHC email 
retention policies. Please save or store appropriately in accordance with 
policy.

This e-mail message, including any attachments, is for the sole use of the 
intended recipient(s) and may contain confidential and privileged information. 
Any unauthorized review, use, disclosure, or distribution is prohibited. If you 
are not the intended recipient, or an employee or agent responsible for 
delivering the message to the intended recipient, please contact the sender by 
reply e-mail and destroy all copies of the original message, including any 
attachments.

___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] IHC Billing - 88342 vs. 88341

[Cartun, Richard via Histonet]

For those of you doing clinical IHC testing, have you been able to automate the 
billing of IHC (88342 vs. 88341) or do you still manual bill?  Our IT staff 
wants to build-in a "88342" and a ""88341" for every antibody that we run and 
then have the pathologist select the appropriate one for billing.

Richard

Richard W. Cartun, MS, PhD
Director, Histology & The Martin M. Berman, MD Immunopathology/Morphologic 
Proteomics Laboratory
Director, Biospecimen Collection Programs
Assistant Director, Anatomic Pathology
Hartford Hospital
80 Seymour Street
Hartford, CT  06102
(860) 972-1596 (Office)
(860) 545-2204 (Fax)
richard.car...@hhchealth.org


This e-mail message, including any attachments, is for the sole use of the 
intended recipient(s) and may contain confidential and privileged information. 
Any unauthorized review, use, disclosure, or distribution is prohibited. If you 
are not the intended recipient, or an employee or agent responsible for 
delivering the message to the intended recipient, please contact the sender by 
reply e-mail and destroy all copies of the original message, including any 
attachments.
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] GD2

[Cartun, Richard via Histonet]

Hello everyone.  Is anyone doing IHC testing for "GD2" on FFPE tissue?  Thank 
you.

Richard

Richard W. Cartun, MS, PhD
Director, Histology & The Martin M. Berman, MD Immunopathology/Morphologic 
Proteomics Laboratory
Director, Biospecimen Collection Programs
Assistant Director, Anatomic Pathology
Hartford Hospital
80 Seymour Street
Hartford, CT  06102
(860) 972-1596 (Office)
(860) 545-2204 (Fax)
richard.car...@hhchealth.org


This e-mail message, including any attachments, is for the sole use of the 
intended recipient(s) and may contain confidential and privileged information. 
Any unauthorized review, use, disclosure, or distribution is prohibited. If you 
are not the intended recipient, or an employee or agent responsible for 
delivering the message to the intended recipient, please contact the sender by 
reply e-mail and destroy all copies of the original message, including any 
attachments.
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] IDH1 Antibody

[Cartun, Richard via Histonet]

If you are doing IHC testing for IDH1 here in the United States, do you get the 
antibody (clone HO9) directly from Dianova in Germany or is there a US 
distributor?  I did try HistoBiotech, but they no longer carry Dianova 
antibodies.  Thank you.

Richard

Richard W. Cartun, MS, PhD
Director, Histology & The Martin M. Berman, MD Immunopathology/Morphologic 
Proteomics Laboratory
Director, Biospecimen Collection Programs
Assistant Director, Anatomic Pathology
Hartford Hospital
80 Seymour Street
Hartford, CT  06102
(860) 972-1596 (Office)
(860) 545-2204 (Fax)
richard.car...@hhchealth.org


This e-mail message, including any attachments, is for the sole use of the 
intended recipient(s) and may contain confidential and privileged information. 
Any unauthorized review, use, disclosure, or distribution is prohibited. If you 
are not the intended recipient, or an employee or agent responsible for 
delivering the message to the intended recipient, please contact the sender by 
reply e-mail and destroy all copies of the original message, including any 
attachments.
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] Lyme Disease

[Cartun, Richard via Histonet]

Is anyone doing PCR testing for Borrelia burgdorferi (Lyme Disease) from 
formalin-fixed, paraffin-embedded tissue?  Thank you!

Richard

Richard W. Cartun, MS, PhD
Director, Histology & The Martin M. Berman, MD Immunopathology/Morphologic 
Proteomics Laboratory
Director, Biospecimen Collection Programs
Assistant Director, Anatomic Pathology
Hartford Hospital
80 Seymour Street
Hartford, CT  06102
(860) 972-1596 (Office)
(860) 545-2204 (Fax)
richard.car...@hhchealth.org


This e-mail message, including any attachments, is for the sole use of the 
intended recipient(s) and may contain confidential and privileged information. 
Any unauthorized review, use, disclosure, or distribution is prohibited. If you 
are not the intended recipient, or an employee or agent responsible for 
delivering the message to the intended recipient, please contact the sender by 
reply e-mail and destroy all copies of the original message, including any 
attachments.
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] IDH1

[Cartun, Richard via Histonet]

For those labs that are performing IDH1 IHC testing on FFPE tissue, which clone 
are you using and where do you get it?  Thank you.

Happy Holidays to everyone.

Richard

Richard W. Cartun, MS, PhD
Director, Histology & The Martin M. Berman, MD Immunopathology/Morphologic 
Proteomics Laboratory
Director, Biospecimen Collection Programs
Assistant Director, Anatomic Pathology
Hartford Hospital
80 Seymour Street
Hartford, CT  06102
(860) 972-1596 (Office)
(860) 545-2204 (Fax)
richard.car...@hhchealth.org


This e-mail message, including any attachments, is for the sole use of the 
intended recipient(s) and may contain confidential and privileged information. 
Any unauthorized review, use, disclosure, or distribution is prohibited. If you 
are not the intended recipient, or an employee or agent responsible for 
delivering the message to the intended recipient, please contact the sender by 
reply e-mail and destroy all copies of the original message, including any 
attachments.
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] Question - EM

[Cartun, Richard via Histonet]

How long can tissue remain in glutaraldehyde before EM testing is performed?  
Thank you.

Richard

Richard W. Cartun, MS, PhD
Director, Histology & The Martin M. Berman, MD Immunopathology & Morphologic 
Proteomics Laboratory
Director, Biospecimen Collection Programs
Assistant Director, Anatomic Pathology
Hartford Hospital
80 Seymour Street
Hartford, CT  06102
(860) 972-1596
(860) 545-2204 Fax


This e-mail message, including any attachments, is for the sole use of the 
intended recipient(s) and may contain confidential and privileged information. 
Any unauthorized review, use, disclosure, or distribution is prohibited. If you 
are not the intended recipient, or an employee or agent responsible for 
delivering the message to the intended recipient, please contact the sender by 
reply e-mail and destroy all copies of the original message, including any 
attachments.
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] Alizarin Red Stain

[Cartun, Richard via Histonet]

Does anyone do the Alizarin Red histochemical stain for calcium in clinical 
samples?  Thank you.

Richard

Richard W. Cartun, MS, PhD
Director, Histology & The Martin M. Berman, MD Immunopathology & Morphologic 
Proteomics Laboratory
Director, Biospecimen Collection Programs
Assistant Director, Anatomic Pathology
Hartford Hospital
80 Seymour Street
Hartford, CT  06102
(860) 972-1596
(860) 545-2204 Fax


This e-mail message, including any attachments, is for the sole use of the 
intended recipient(s) and may contain confidential and privileged information. 
Any unauthorized review, use, disclosure, or distribution is prohibited. If you 
are not the intended recipient, or an employee or agent responsible for 
delivering the message to the intended recipient, please contact the sender by 
reply e-mail and destroy all copies of the original message, including any 
attachments.
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

Re: [Histonet] Unstained slides

[Cartun, Richard via Histonet]

It appears that the presence of water, both endogenously and exogenously, plays 
a central role in the loss of antigenicity in stored unstained slides (see 
reference below).  Labs that are experiencing significant loss of 
immunoreactivity in their unstained slides should check their tissue processing.

Xie R, Chung J-Y, Ylaya K, et al.:  Factors influencing the degradation of 
archival formalin-fixed, paraffin-embedded tissue sections.  J of Histochem 
Cytochem 2011; 59:356-365.

Richard

Richard W. Cartun, MS, PhD
Director, Histology & The Martin M. Berman, MD Immunopathology & Morphologic 
Proteomics Laboratory
Director, Biospecimen Collection Programs
Assistant Director, Anatomic Pathology
Hartford Hospital
80 Seymour Street
Hartford, CT  06102
(860) 972-1596
(860) 545-2204 Fax

-Original Message-
From: Rene J Buesa via Histonet [mailto:histonet@lists.utsouthwestern.edu]
Sent: Sunday, August 19, 2018 2:09 PM
To: Frazier, John; Terri Braud
Cc: histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Unstained slides

This is an email from Outside HHC. USE CAUTION opening attachments or links 
from unknown senders.

Everything has been pointed out is correct BUT also pivot on "how the unstained 
slides are kept".Kept in a box their "useful life" is quite short (not beyond 1 
week at the most).Kept at -80ºC I have used them after years of being stored 
the principle being of deep-freezing and this is "standard procedure" for IDF 
"+ controls".Kept in a Coplin jar filled with mineral oil or paraffin covered I 
have used them after months of being stored the principle being that, isolated 
from air oxygen, epitopes do not oxidize ("weaken") of if they do, the rate is 
greatly slowed.On the other hand, usually, unstained slides are kept for only 
few days in the event that, lets say within a week, the PT decides to order 
some special procedure and sometimes it is impossible "return" to the original 
block many times "almost exhausted".Properly done storing unstained slides are 
extremely useful.René

On Sunday, August 19, 2018 1:52 PM, "Frazier, John via Histonet" 
 wrote:


 I agree with Tim as well. This is what we advise our clients to do. It takes 
some coordination with the pathologist, but it is the best strategy for 
reducing unnecessary unstained slides. In the studies that we have performed 
only 10% of the unstained slides that are cut are you and the 90% are are it 
takes some coordination with the pathologist, but it is the best strategy for 
reducing unnecessary unstained slides. In the studies that we have performed 
only 10% of the unstained slides that are cut are you and 90% are thrown away 
thrown away.
Several laboratories that I have visited in order to reduce the amount of 
wasted tissue when refacing the blocks, is to reseal the blocks with liquid 
paraffin, that have scant or small amounts of tissue in the block, such as the 
needle core biopsy.
Bottom line on this issue is to educate the pathologist, and not water and 
stain slides except in rare occasions

Sent from my iPhone

> On Aug 17, 2018, at 14:07, Terri Braud  wrote:
>
> I'm with Tim Morken on this one. The variability of antigenicity in storage 
> is so wide open, and there really is no recent data, so we just make a point 
> of educating our techs on not wasting tissue/levels during sectioning.  If 
> the techs feel that the residual tissue in the block is in danger of being 
> exhausted, we communicate with our pathologists on how best to handle any 
> requests.  Unstained slides was time, money, and storage and we are better 
> off without them.
>
> Terri L. Braud, HT(ASCP)
> Anatomic Pathology Supervisor
> Laboratory
> Holy Redeemer Hospital
> 1648 Huntingdon Pike
> Meadowbrook, PA 19046
> ph: 215-938-3689
> fax: 215-938-3874
> Care, Comfort, and Heal
>
> Today's Topics:
>  7. Re: Unstained slides - how long are they good for?
>  (Morken, Timothy)
>
> Message: 7
> Date: Fri, 17 Aug 2018 15:16:00 +
> From: "Morken, Timothy" 
> To: P Sicurello 
> Subject: Re: [Histonet] Unstained slides - how long are they good for?
>
>
> Paula, since it is variable we strive to not have unstained slides. We had 
> kept them indefinitely, then when storage was overwhelming us we reduced it 
> to 2 months maximum. Now we require request for unstained to be ordered in 
> the system and delivered to the pathologist. We do not hold any in the lab. 
> We recut when new stains are ordered. In the past we had routinely cut extras 
> "just in case" but ended up with thousands of unstained slides that were 
> never used. Instead we trained everyone to reduce wastage and get good 
> sections from a cut block with minimal facing. We have not stored unstained 
> sections for many years and they do not seem to be missed.
>
> Tim Morken
> Pathology Site Manager, Parnassus
> Supervisor, Electron Microscopy/Neuromuscular Special Studies
> Department of Pathology UC San Francisco Medical Center
>
>
> -Original Message-
> 

[Histonet] Decalcification for Bone Marrow Biopsies

[Cartun, Richard via Histonet]

Help!  For years we have used Decal-Stat for decalcifying our bone marrow 
biopsies with good results.  For the past month we have been having problems 
with tissue loss and morphological damage with these specimens following 
decalcification.  Unfortunately, this was just brought to my attention.  
Someone told me this morning that the company producing this product was sold 
and the formulation may have changed.  Is that true?

Richard

Richard W. Cartun, MS, PhD
Director, Histology & The Martin M. Berman, MD Immunopathology & Morphologic 
Proteomics Laboratory
Director, Biospecimen Collection Programs
Assistant Director, Anatomic Pathology
Hartford Hospital
80 Seymour Street
Hartford, CT  06102
(860) 972-1596
(860) 545-2204 Fax


This e-mail message, including any attachments, is for the sole use of the 
intended recipient(s) and may contain confidential and privileged information. 
Any unauthorized review, use, disclosure, or distribution is prohibited. If you 
are not the intended recipient, or an employee or agent responsible for 
delivering the message to the intended recipient, please contact the sender by 
reply e-mail and destroy all copies of the original message, including any 
attachments.
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

Re: [Histonet] LEF1 - antibody

[Cartun, Richard via Histonet]

We use a RTU (clone EP310) from Cell Marque on the Leica Bond.  Antigen 
retrieval is "high pH" HIER for 20 minutes.

Richard

Richard W. Cartun, MS, PhD
Director, Histology & The Martin M. Berman, MD Immunopathology & Morphologic 
Proteomics Laboratory
Director, Biospecimen Collection Programs
Assistant Director, Anatomic Pathology
Hartford Hospital
80 Seymour Street
Hartford, CT  06102
(860) 972-1596
(860) 545-2204 Fax


-Original Message-
From: Alminawi, Samira via Histonet [mailto:histonet@lists.utsouthwestern.edu]
Sent: Friday, May 25, 2018 10:29 AM
To: Histonet@lists.utsouthwestern.edu
Subject: [Histonet] LEF1 - antibody

This email is from outside HHC. BE CAREFUL when opening attachments or links 
from unknown senders.

Hi,
Is anybody using LEF1 antibody and what is the recommended protocol?
Thank you for help


Samira Alminawi, MLT
Senior- Immunohistochemistry
Sunnybrook Health Sciences Centre
2075 Bayview Avenue, Toronto, ON M4N 3M5
Tel: 416-480-6100 ext:1666
e-mail: samira.almin...@sunnybrook.ca

This e-mail is intended only for the named recipient(s) and may contain 
confidential, personal and/or health information (information which may be 
subject to legal restrictions on use, retention and/or disclosure).  No waiver 
of confidence is intended by virtue of communication via the internet.  Any 
review or distribution by anyone other than the person(s) for whom it was 
originally intended is strictly prohibited.  If you have received this e-mail 
in error, please contact the sender and destroy all copies.

___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet



Reminder: This e-mail and any attachments are subject to the current HHC email 
retention policies. Please save or store appropriately in accordance with 
policy.

This e-mail message, including any attachments, is for the sole use of the 
intended recipient(s) and may contain confidential and privileged information. 
Any unauthorized review, use, disclosure, or distribution is prohibited. If you 
are not the intended recipient, or an employee or agent responsible for 
delivering the message to the intended recipient, please contact the sender by 
reply e-mail and destroy all copies of the original message, including any 
attachments.

___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] COnsult Reporting

[Cartun, Richard via Histonet]

I know I asked this question before, but I can't find my notes.  If you provide 
a diagnostic consultation on pathology slides, do you need a separate report 
with a new "Date-of-Service" (the date that the consult is requested) for 
reporting and billing purposes?  Thank you.

Richard

Richard W. Cartun, MS, PhD
Director, Histology & The Martin M. Berman, MD Immunopathology & Morphologic 
Proteomics Laboratory
Director, Biospecimen Collection Programs
Assistant Director, Anatomic Pathology
Hartford Hospital
80 Seymour Street
Hartford, CT  06102
(860) 972-1596
(860) 545-2204 Fax


This e-mail message, including any attachments, is for the sole use of the 
intended recipient(s) and may contain confidential and privileged information. 
Any unauthorized review, use, disclosure, or distribution is prohibited. If you 
are not the intended recipient, or an employee or agent responsible for 
delivering the message to the intended recipient, please contact the sender by 
reply e-mail and destroy all copies of the original message, including any 
attachments.
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

Re: [Histonet] Cold Ischemic interval

[Cartun, Richard via Histonet]

We are using the "ASCO/CAP" guidelines for all cancer resection specimens.

Richard

Richard W. Cartun, MS, PhD
Director, Histology and the Martin M. Berman, MD Immunopathology and 
Morphologic Proteomics Laboratory
Director, Biospecimen Collection Programs
Assistant Director, Anatomic Pathology
Department of Pathology and Laboratory Medicine
Hartford Hospital
80 Seymour Street
Hartford, CT  06102
(860) 972-1596 Office
(860) 545-2204 Fax


From: Amy Johnson via Histonet [histonet@lists.utsouthwestern.edu]
Sent: Thursday, January 18, 2018 2:48 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Cold Ischemic interval

EXTERNAL email from Outside HHC! Do NOT open attachments or click links from 
unknown senders.

Does the cold ischemic interval pertain only to breast specimens that have not 
have receptor studies done or does it pertain to all breast specimen, i.e. 
needle core biopsies, lumpectomies, mastectomies?

Thanks for you help


Amy

Amylin Johnson, B.S. HTL(ASCP)
Associates in Pathology
Wausau Wi 54401
715-847-2130


___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet



Reminder: This e-mail and any attachments are subject to the current HHC email 
retention policies. Please save or store appropriately in accordance with 
policy.

This e-mail message, including any attachments, is for the sole use of the 
intended recipient(s) and may contain confidential and privileged information. 
Any unauthorized review, use, disclosure, or distribution is prohibited. If you 
are not the intended recipient, or an employee or agent responsible for 
delivering the message to the intended recipient, please contact the sender by 
reply e-mail and destroy all copies of the original message, including any 
attachments.

___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

Re: [Histonet] Extra unstained paraffin embedded tissue slides

[Cartun, Richard via Histonet]

We file our unstains separately and then discard after one year.

Richard

Richard W. Cartun, MS, PhD
Director, Histology & The Martin M. Berman, MD Immunopathology & Morphologic 
Proteomics Laboratory
Director, Biospecimen Collection Programs
Assistant Director, Anatomic Pathology
Hartford Hospital
80 Seymour Street
Hartford, CT  06102
(860) 972-1596
(860) 545-2204 Fax

-Original Message-
From: Kristina Johnson via Histonet [mailto:histonet@lists.utsouthwestern.edu]
Sent: Thursday, January 04, 2018 9:35 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Extra unstained paraffin embedded tissue slides

EXTERNAL email from Outside HHC! Do NOT open attachments or click links from 
unknown senders.

Good morning fellow Histo-peeps,
I am needing to see how you file your extra unstained/ un-coverslipped paraffin 
tissue slides.  You know the slides that were cut in case special stains were 
ordered but were never needed, or that were cut while cutting levels for 
alopecias or fine needle bx. and not stained.  I have always filed them 
unstained, and un-coverslipped along with the remainder of the case number that 
had been stained and coverslipped. What do you do with your extra slides?

--



*Kristina Johnson,  HT (ASCP**)*

Histologist

Moeller Dermatology, LLC

1911 N. Webb Road

Wichita, KS 67206

☏ (316)682-7546 Ext:246  (316)682-7554

www.moellerdermatology.com

Facebook 
Twitter 



This e-mail, and any attachments thereto, is intended only for use by the
addressee(s) named herein and may contain legally privileged and/or 
confidential information. If you are not the intended recipient of this e-mail 
(or the person responsible for delivering this document to the intended 
recipient), you are hereby notified that any dissemination, distribution, 
printing or copying of this e-mail, and any attachments thereto, is strictly 
prohibited. If you have received this e-mail in error, please respond to the 
individual sending the message and permanently delete the original and any copy 
of any e-mail and any printout thereof 
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet


Reminder: This e-mail and any attachments are subject to the current HHC email 
retention policies. Please save or store appropriately in accordance with 
policy.

This e-mail message, including any attachments, is for the sole use of the 
intended recipient(s) and may contain confidential and privileged information. 
Any unauthorized review, use, disclosure, or distribution is prohibited. If you 
are not the intended recipient, or an employee or agent responsible for 
delivering the message to the intended recipient, please contact the sender by 
reply e-mail and destroy all copies of the original message, including any 
attachments.
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

Re: [Histonet] Beta-Amyloid antibody for IHC

[Cartun, Richard via Histonet]

No, I would like to use “6E10” if it’s still available.  Thank you!

Richard

From: Colleen Forster [mailto:cfors...@umn.edu]
Sent: Wednesday, December 27, 2017 4:23 PM
To: Cartun, Richard
Cc: histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Beta-Amyloid antibody for IHC

CAUTION: This email is from outside HHC. USE CARE when opening attachments or 
links.

Do you knot want to use 6E10 anymore? I believe you can still get it from 
Biolegends?

https://www.biolegend.com/en-us/products/purified-anti-beta-amyloid--1-16-antibody-11228
Respectfully,
Colleen Forster  HT(ASCP)QIHC
University of Minnesota

On Wed, Dec 27, 2017 at 3:20 PM, Cartun, Richard via Histonet 
<histonet@lists.utsouthwestern.edu<mailto:histonet@lists.utsouthwestern.edu>> 
wrote:
We are looking for a good antibody to detect Beta-Amyloid in formalin-fixed, 
paraffin-embedded human brain tissue.  Any recommendations?  We have been using 
the clone "6E10" which we purchased from Signet Laboratories back in 2001.  
Thank you!

Richard

Richard W. Cartun, MS, PhD
Director, Histology & The Martin M. Berman, MD Immunopathology & Morphologic 
Proteomics Laboratory
Director, Biospecimen Collection Programs
Assistant Director, Anatomic Pathology
Hartford Hospital
80 Seymour Street
Hartford, CT  06102
(860) 972-1596<tel:%28860%29%20972-1596>
(860) 545-2204<tel:%28860%29%20545-2204> Fax


This e-mail message, including any attachments, is for the sole use of the 
intended recipient(s) and may contain confidential and privileged information. 
Any unauthorized review, use, disclosure, or distribution is prohibited. If you 
are not the intended recipient, or an employee or agent responsible for 
delivering the message to the intended recipient, please contact the sender by 
reply e-mail and destroy all copies of the original message, including any 
attachments.
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu<mailto:Histonet@lists.utsouthwestern.edu>
http://lists.utsouthwestern.edu/mailman/listinfo/histonet



Reminder: This e-mail and any attachments are subject to the current HHC email 
retention policies. Please save or store appropriately in accordance with 
policy.
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] Beta-Amyloid antibody for IHC

[Cartun, Richard via Histonet]

We are looking for a good antibody to detect Beta-Amyloid in formalin-fixed, 
paraffin-embedded human brain tissue.  Any recommendations?  We have been using 
the clone "6E10" which we purchased from Signet Laboratories back in 2001.  
Thank you!

Richard

Richard W. Cartun, MS, PhD
Director, Histology & The Martin M. Berman, MD Immunopathology & Morphologic 
Proteomics Laboratory
Director, Biospecimen Collection Programs
Assistant Director, Anatomic Pathology
Hartford Hospital
80 Seymour Street
Hartford, CT  06102
(860) 972-1596
(860) 545-2204 Fax


This e-mail message, including any attachments, is for the sole use of the 
intended recipient(s) and may contain confidential and privileged information. 
Any unauthorized review, use, disclosure, or distribution is prohibited. If you 
are not the intended recipient, or an employee or agent responsible for 
delivering the message to the intended recipient, please contact the sender by 
reply e-mail and destroy all copies of the original message, including any 
attachments.
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

Re: [Histonet] ROS1

[Cartun, Richard via Histonet]

Yes, we've had excellent results using their clone "D4D6".  We use it at 1:50 
dilution on the Leica Bond Max following high-pH retrieval.  Please contact me 
directly if you require additional information.

Richard

Richard W. Cartun, MS, PhD
Director, Histology & The Martin M. Berman, MD Immunopathology & Morphologic 
Proteomics Laboratory
Director, Biospecimen Collection Programs
Assistant Director, Anatomic Pathology
Hartford Hospital
80 Seymour Street
Hartford, CT  06102
(860) 972-1596
(860) 545-2204 Fax

-Original Message-
From: Martin, Erin via Histonet [mailto:histonet@lists.utsouthwestern.edu]
Sent: Monday, December 11, 2017 5:38 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] ROS1

This email is from outside HHC. BE CAREFUL when opening attachments or links 
from unknown senders.

Good morning histoland!


Has anyone had success with the ROS1 antibody from CellSignaling?  I just can't 
seem to get it working beyond what our pathologist call "a faint blush".  I 
would greatly appreciate if you would be wiling to share your protocol!


Have a lovely Monday!

Erin


Erin Martin, Histology Supervisor
UCSF  Dermatopathology and Oral Pathology Service
1701 Divisadero St, San Francisco, CA 94044
415-353-7248

Confidentiality Notice
The information transmitted is intended only for the person or entity to which 
it is addressed and may contain confidential and/or privileged material.  Any 
review, retransmission, dissemination or other use of, or taking of any action 
in reliance upon, this information by persons or entities other than the 
intended recipient is prohibited.  If you receive this in error please contact 
the sender and delete the material from any computer.
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet



Reminder: This e-mail and any attachments are subject to the current HHC email 
retention policies. Please save or store appropriately in accordance with 
policy.

This e-mail message, including any attachments, is for the sole use of the 
intended recipient(s) and may contain confidential and privileged information. 
Any unauthorized review, use, disclosure, or distribution is prohibited. If you 
are not the intended recipient, or an employee or agent responsible for 
delivering the message to the intended recipient, please contact the sender by 
reply e-mail and destroy all copies of the original message, including any 
attachments.

___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] I am trying to contact ....

[Cartun, Richard via Histonet]

Diane Cadorette-Hall.

Richard

Richard W. Cartun, MS, PhD
Director, Histology & The Martin M. Berman, MD Immunopathology & Morphologic 
Proteomics Laboratory
Director, Biospecimen Collection Programs
Assistant Director, Anatomic Pathology
Hartford Hospital
80 Seymour Street
Hartford, CT  06102
(860) 972-1596
(860) 545-2204 Fax


This e-mail message, including any attachments, is for the sole use of the 
intended recipient(s) and may contain confidential and privileged information. 
Any unauthorized review, use, disclosure, or distribution is prohibited. If you 
are not the intended recipient, or an employee or agent responsible for 
delivering the message to the intended recipient, please contact the sender by 
reply e-mail and destroy all copies of the original message, including any 
attachments.
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

Re: [Histonet] EDTA decalcification tissue issues

[Cartun, Richard via Histonet]

We switched from acid decal to EDTA recently; primarily for molecular testing.  
We are starting to see some excellent results.  At first, we were not decaling 
long enough (EDTA decalcification is a very slow process).  We also use the 
Leica Bond Max and we have not had any problems with tissue loss.  We are only 
using EDTA for small biopsies.  Please not that these specimens must be fixed 
adequately before the decal process is started.

Richard

Richard W. Cartun, MS, PhD
Director, Histology & The Martin M. Berman, MD Immunopathology & Morphologic 
Proteomics Laboratory
Director, Biospecimen Collection Programs
Assistant Director, Anatomic Pathology
Hartford Hospital
80 Seymour Street
Hartford, CT  06102
(860) 972-1596
(860) 545-2204 Fax

-Original Message-
From: Pairan, Kelly via Histonet [mailto:histonet@lists.utsouthwestern.edu]
Sent: Friday, November 10, 2017 6:39 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] EDTA decalcification tissue issues

This email is from outside HHC. BE CAREFUL when opening attachments or links 
from unknown senders.

Good Morning,

Recently, our lab has been working on validating an EDTA method of 
decalcification.  When we ran the IHC's on the decalcified bone block, the 
majority of the tissue lifted off the slide.  We use the Leica Bonds for our 
IHC staining.  Does anyone else have a hard time getting EDTA decalcified 
tissue to stay on positively charged slides during IHC runs?  Do you have a 
trick you use?  We really did not have this big of a problem when we were 
decalcifying with Formical or Nitrical (big bones only).


Thanks for your help,

?Kelly Pairan, HT(ASCP), QIHC(ASCP)
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet



Reminder: This e-mail and any attachments are subject to the current HHC email 
retention policies. Please save or store appropriately in accordance with 
policy.

This e-mail message, including any attachments, is for the sole use of the 
intended recipient(s) and may contain confidential and privileged information. 
Any unauthorized review, use, disclosure, or distribution is prohibited. If you 
are not the intended recipient, or an employee or agent responsible for 
delivering the message to the intended recipient, please contact the sender by 
reply e-mail and destroy all copies of the original message, including any 
attachments.

___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] Announcing the 12th annual retreat of applied immunohistochemistry and molecular pathology at the beautiful Marriott, Key Largo, FL

[Cartun, Richard via Histonet]

I am forwarding this exciting meeting announcement to the Histonet Community.

Richard

Richard W. Cartun, MS, PhD
Director, Histology & The Martin M. Berman, MD Immunopathology & Morphologic 
Proteomics Laboratory
Director, Biospecimen Collection Programs
Assistant Director, Anatomic Pathology
Hartford Hospital
80 Seymour Street
Hartford, CT  06102
(860) 972-1596
(860) 545-2204 Fax

Following pre-hurricane preparation and 2 weeks of lack of electricity 
post-hurricane, we’re finally able to get our lives back on track, hence this 
delayed announcement for the 12th annual AIMP retreat. Please forward this 
email to as many folks as you can. Those of you who attended previous retreats 
will receive a decent discount as specified on the website.

Please let me know if you have any questions. I hope to see you in the FL Keyes 
in February.

Best regards,

Hadi

Hadi Yaziji, MD, FCAP, FASCP
Medical Director
Vitro Molecular Laboratories
8700 W. Flagler Street, Suite 100  l   Miami, FL 33174

www.vitromolecular.com
hyaz...@vitromolecular.com
Office 305-267-7979  l   Fax 786-513-0175


[https://gallery.mailchimp.com/b4d3e9ff192b16fd68e3363a0/images/9e4a48fa-48d5-43fe-ac8c-f45b596f7767.jpg]





12th Annual International Retreat on Applied Immunohistochemistry & Molecular 
Pathology

Previous AIMP retreat attendees have attested that AIMP has been “the best 
pathology CME event ever attended”. Our 12th annual retreat will have the 
following list of speakers (arranged alphabetically):

Noah Brown, MD; University of Michigan
Richard Cartun, PhD; Hartford Hospital
Carol Cheung, MD, PhD., JD, FRCPC, University of Toronto
Brenda Cox, FHFMA, CPC, MT (ASCP)
Richard Eisen, MD; Banner Health
Joel Greenson, MD; University of Michigan
Jason Hornick, MD, PhD; Harvard University
Jeffrey Myers, MD; University of Michigan
Emina Terlacovich, MD, PhD; University of Saskatchewan
Megan Troxell, MD; Stanford University
Hadi Yaziji, MD, Vitro Molecular Laboratories

Location: Key Largo Marriott (Key Largo, FL, USA)
Date: February 4 - February 8, 2018

Topics at a glance:

Sunday, February 4
Welcome and Opening Remarks [Hadi Yaziji]
Pre-Test [Hadi Yaziji]
Interpreting FISH Assays by The Practicing Pathologist - General Principles  
[Hadi Yaziji]
Best of USCAP 2017: IHC Applications [Richard Eisen]
Developing Fit-For-Purpose IHC Assays [Carol Cheung]
Validation vs. Verification of IHC Assays:  The Clue is in the Test Performance 
Characteristics [Emina Torlakovic]
Tissue Tools are Power Tools for the IHC Laboratory.  [Carol Cheung]
Controlling the Controls in IHC – a Path to Standardization [Emina Torlakovic]

Monday, February 5:
Malignant Mesothelioma and Other Diffuse Pleural Tumors [Jeffrey Myers]
Role of the Pathologist in Diagnosis and Management of Patients with NSCLC 
[Jeffrey Myers]
Interpreting FISH Assays: Common Assays & Specific Scenarios  [Hadi Yaziji]
Molecular Genetics of Colon Cancer, what a practicing surgical pathologist 
needs to know [Joel Greenson]
Specimen Considerations for Solid Tumor Molecular Testing [Noah Brown]
PD-L1: Interpretation Pitfalls & Update on Utility as a Companion Diagnostic 
Assay [Hadi Yaziji]

Tuesday, February 6:
HER2 Testing: 2018 ASCO/CAP Guidelines - What’s New and Why [Hadi Yaziji]
Troubleshooting Diagnostic Immunohistochemistry [Rich Cartun]
Open Mic: Bring Your Own Questions - Part I  [Hadi Yaziji]
Best “Specialized” Markers (Myoepithelial, Cytokeratins, Neuroendocrine, 
Endothelial, Histocytic, Megakaryocitic, Erythroid, ETC) in IHC [Hadi Yaziji]
Surgical Pathology Case Presentations - Session 1 [Faculty]
Surgical Pathology Case Presentations - Session 2 [Faculty]

Wednesday February 7:
The Evolution of IHC for Soft Tissue Tumors in the 21st Century: From 
Differentiation to Molecular Genetics [Hornick]
Immunohistochemistry in Kidney Tumors: The New WHO Classification and Beyond 
[Megan Troxell]
Beyond Lineage: Diagnostic and Predictive Molecular IHC for Surgical 
Pathologists [Jason Hornick]
Immunohistochemistry in Transplant Pathology [Megan Troxell]
Surgical Pathology Case Presentations - Session 3 [Faculty]
Surgical Pathology Case Presentations - Session 4 [Faculty]

Thursday, February 8:
Essential Coding & Compliance: 2018 Update - Part I [Brenda Cox]
Essential Coding & Compliance: 2018 Update - Part II [Brenda Cox]
Open Mic: Bring Your Own Questions - Part II  [Hadi Yaziji]
Open Mic: Bring Your Own Questions - Part III  [Hadi Yaziji]
Surgical Pathology Case Presentations - Session 5 [Faculty]
Surgical Pathology Case Presentations - Session 6 [Faculty]

To register for the retreat or for any other information, please visit us 
online at 
pathlearning.com
For any other questions or inquiries, please email us at 
i...@pathlearning.com For complete information of 
venue, retreat 

[Histonet] Mass Spectrometry

[Cartun, Richard via Histonet]

Anyone working in Anatomic Pathology and using mass spectrometry for protein 
analysis on cancer cells?  Thank you!

Richard

Richard W. Cartun, MS, PhD
Director, Histology & The Martin M. Berman, MD Immunopathology & Morphologic 
Proteomics Laboratory
Director, Biospecimen Collection Programs
Assistant Director, Anatomic Pathology
Hartford Hospital
80 Seymour Street
Hartford, CT  06102
(860) 972-1596
(860) 545-2204 Fax


This e-mail message, including any attachments, is for the sole use of the 
intended recipient(s) and may contain confidential and privileged information. 
Any unauthorized review, use, disclosure, or distribution is prohibited. If you 
are not the intended recipient, or an employee or agent responsible for 
delivering the message to the intended recipient, please contact the sender by 
reply e-mail and destroy all copies of the original message, including any 
attachments.
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

Re: [Histonet] CD35

[Cartun, Richard via Histonet]

We are now using Epitomics' clone EPR6602 with great results.  I don't know if 
it's offered as a "RTU", but I would check with Cell Marque or abcam; they 
carry Epitomics' clones

Richard

Richard W. Cartun, MS, PhD
Director, Histology & The Martin M. Berman, MD Immunopathology & Morphologic 
Proteomics Laboratory
Director, Biospecimen Collection Programs
Assistant Director, Anatomic Pathology
Hartford Hospital
80 Seymour Street
Hartford, CT  06102
(860) 972-1596
(860) 545-2204 Fax



-Original Message-
From: Pairan, Kelly via Histonet [mailto:histonet@lists.utsouthwestern.edu]
Sent: Wednesday, July 05, 2017 12:53 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] CD35

Hi Everyone,
I hope you all had a lovely 4th of July.  Is anyone out there using a CD35 RTU 
antibody that they like? The vendor we normally use has had the item on 
backorder for over a month and a half with no end in sight.

Thanks,
Kelly Pairan, HTCM(ASCP)
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

This e-mail message, including any attachments, is for the sole use of the 
intended recipient(s) and may contain confidential and privileged information. 
Any unauthorized review, use, disclosure, or distribution is prohibited. If you 
are not the intended recipient, or an employee or agent responsible for 
delivering the message to the intended recipient, please contact the sender by 
reply e-mail and destroy all copies of the original message, including any 
attachments.

___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] ClearRite

[Cartun, Richard via Histonet]

Anyone using ClearRite (xylene replacement) for tissue processing?  If so, 
what's been your experience?  Any effect on IHC or molecular testing?  Thank 
you!

Richard

Richard W. Cartun, MS, PhD
Director, Histology & The Martin M. Berman, MD Immunopathology & Morphologic 
Proteomics Laboratory
Director, Biospecimen Collection Programs
Assistant Director, Anatomic Pathology
Hartford Hospital
80 Seymour Street
Hartford, CT  06102
(860) 972-1596
(860) 545-2204 Fax


This e-mail message, including any attachments, is for the sole use of the 
intended recipient(s) and may contain confidential and privileged information. 
Any unauthorized review, use, disclosure, or distribution is prohibited. If you 
are not the intended recipient, or an employee or agent responsible for 
delivering the message to the intended recipient, please contact the sender by 
reply e-mail and destroy all copies of the original message, including any 
attachments.
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] Question

[Cartun, Richard via Histonet]

We are thinking of changing the way we report consult testing (IHC, Flow 
Cytometry, and Molecular) for our system hospitals.  Instead of accessioning 
the consult case here, the requesting hospital would create a 
"Procedure/Addendum" in Sunquest CoPath for their specimen and then send us the 
unstained slides or paraffin block for testing.  Once the testing is completed, 
the pathologist here (assigned to the case) would go into the other hospital's  
report, populate the "Procedure/Addendum" with the testing performed and the 
interpretation, and then sign it out.  We would not have a consult accession 
number (or report) here at our hospital for this specimen.  Does this make 
sense?  Is anyone else in a multiple hospital system already doing this?  I 
welcome all comments.  Thank you.

Richard

Richard W. Cartun, MS, PhD
Director, Histology & The Martin M. Berman, MD Immunopathology & Morphologic 
Proteomics Laboratory
Director, Biospecimen Collection Programs
Assistant Director, Anatomic Pathology
Hartford Hospital
80 Seymour Street
Hartford, CT  06102
(860) 972-1596
(860) 545-2204 Fax


This e-mail message, including any attachments, is for the sole use of the 
intended recipient(s) and may contain confidential and privileged information. 
Any unauthorized review, use, disclosure, or distribution is prohibited. If you 
are not the intended recipient, or an employee or agent responsible for 
delivering the message to the intended recipient, please contact the sender by 
reply e-mail and destroy all copies of the original message, including any 
attachments.
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] IHC testing for Parvovirus (Human)

[Cartun, Richard via Histonet]

If you do this test, where do you get your antibody?  Thank you.

Richard

Richard W. Cartun, MS, PhD
Director, Histology & The Martin M. Berman, MD Immunopathology & Morphologic 
Proteomics Laboratory
Director, Biospecimen Collection Programs
Assistant Director, Anatomic Pathology
Hartford Hospital
80 Seymour Street
Hartford, CT  06102
(860) 972-1596
(860) 545-2204 Fax


This e-mail message, including any attachments, is for the sole use of the 
intended recipient(s) and may contain confidential and privileged information. 
Any unauthorized review, use, disclosure, or distribution is prohibited. If you 
are not the intended recipient, or an employee or agent responsible for 
delivering the message to the intended recipient, please contact the sender by 
reply e-mail and destroy all copies of the original message, including any 
attachments.
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

Re: [Histonet] Send-Outs

[Cartun, Richard via Histonet]

Do you have a Pathology Residency Program?  If so, good luck with a policy that 
states that requests will be sent out in 48 hours.  Our policy is 3-5 business 
days once we receive the request.  Having said that, most requests are 
processed in 2-3 days.

Richard

Richard W. Cartun, MS, PhD
Director, Histology & The Martin M. Berman, MD Immunopathology & Morphologic 
Proteomics Laboratory
Director, Biospecimen Collection Programs
Assistant Director, Anatomic Pathology
Hartford Hospital
80 Seymour Street
Hartford, CT  06102
(860) 972-1596
(860) 545-2204 Fax



-Original Message-
From: Morken, Timothy via Histonet [mailto:histonet@lists.utsouthwestern.edu]
Sent: Tuesday, May 16, 2017 11:53 AM
To: Cristi Rigazio
Cc: Histonet
Subject: Re: [Histonet] Send-Outs

Really? You want to compare your lab to Amazon? If you have stock on-hand, 
located in one place, have robots to retrieve it, people who do nothing but 
pack boxes and trucks standing by to take any order at any time of day, then 
you are in the league of Amazon and can receive an order and send it out within 
an hour.

However most of us first need to find the material to send out - slides? where 
are they? Pathologist, resident? waiting to be filed? Removed from file by 
someone else? Can't find anything in THAT office...Same with blocks - not in 
file, is there a card telling who took it? Maybe for recuts, maybe for 
research, then back into the batch to be filed. Look 5 different places. Frozen 
tissue is usually easier but, whoops...already sent some out for another 
requested test...

Anyway, that is what my experience is!

We don't have any set time requirement. It could be an hour or a few days 
depending on if can easily find the material they want to send. Usually it is 
within a day.


Tim Morken
Pathology Site Manager, Parnassus
Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of 
Pathology UC San Francisco Medical Center



-Original Message-
From: Cristi Rigazio via Histonet [mailto:histonet@lists.utsouthwestern.edu]
Sent: Monday, May 15, 2017 10:55 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Send-Outs

Hi Histoland!

We have been reviewing our send out procedures and I have been tasked with 
researching what TAT's other institutions define.  Currently, we allow 48 hours 
for the preparation of "orders" to the time sent out.  Is this reasonable?  Do 
other facilities allow more or less time?

I feel like Amazon can get something to my door step in a matter of hours, so I 
must be missing something obvious in our steps that could be simplified, but I 
can't put my finger on it.  Any feedback on what others are doing is greatly 
appreciated.

Sincerely,
Cristi
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

This e-mail message, including any attachments, is for the sole use of the 
intended recipient(s) and may contain confidential and privileged information. 
Any unauthorized review, use, disclosure, or distribution is prohibited. If you 
are not the intended recipient, or an employee or agent responsible for 
delivering the message to the intended recipient, please contact the sender by 
reply e-mail and destroy all copies of the original message, including any 
attachments.

___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] NGS Testing

[Cartun, Richard via Histonet]

I know this question is somewhat out of our domain, but are any of you engaged 
in "Next Generation Sequencing" (NGS) testing on patient FFPE cancer specimens? 
 If so, what's your experience with insurance pre-certification and 
reimbursement?  Thank you as always.

Richard

Richard W. Cartun, MS, PhD
Director, Histology & The Martin M. Berman, MD Immunopathology & Morphologic 
Proteomics Laboratory
Director, Biospecimen Collection Programs
Assistant Director, Anatomic Pathology
Hartford Hospital
80 Seymour Street
Hartford, CT  06102
(860) 972-1596
(860) 545-2204 Fax


This e-mail message, including any attachments, is for the sole use of the 
intended recipient(s) and may contain confidential and privileged information. 
Any unauthorized review, use, disclosure, or distribution is prohibited. If you 
are not the intended recipient, or an employee or agent responsible for 
delivering the message to the intended recipient, please contact the sender by 
reply e-mail and destroy all copies of the original message, including any 
attachments.
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] Pathology Slides Send-Outs

[Cartun, Richard via Histonet]

I'm wondering if any of you take a patient's credit card to pay for the FedEx 
shipping of pathology slides when they request that their slides be sent to 
another hospital or medical institution?  Thank you.

Richard

Richard W. Cartun, MS, PhD
Director, Histology & The Martin M. Berman, MD Immunopathology & Morphologic 
Proteomics Laboratory
Director, Biospecimen Collection Programs
Assistant Director, Anatomic Pathology
Hartford Hospital
80 Seymour Street
Hartford, CT  06102
(860) 972-1596
(860) 545-2204 Fax


This e-mail message, including any attachments, is for the sole use of the 
intended recipient(s) and may contain confidential and privileged information. 
Any unauthorized review, use, disclosure, or distribution is prohibited. If you 
are not the intended recipient, or an employee or agent responsible for 
delivering the message to the intended recipient, please contact the sender by 
reply e-mail and destroy all copies of the original message, including any 
attachments.
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] ICD-10 Codes

[Cartun, Richard via Histonet]

What do you do when you receive a pathology consult and it is missing an ICD-10 
code?  I am being told that we cannot accession it until we get a code from the 
submitting doctor's office, and that we cannot add one.  Thank you.

Richard

Richard W. Cartun, MS, PhD
Director, Histology & The Martin M. Berman, MD Immunopathology & Morphologic 
Proteomics Laboratory
Director, Biospecimen Collection Programs
Assistant Director, Anatomic Pathology
Hartford Hospital
80 Seymour Street
Hartford, CT  06102
(860) 972-1596
(860) 545-2204 Fax


This e-mail message, including any attachments, is for the sole use of the 
intended recipient(s) and may contain confidential and privileged information. 
Any unauthorized review, use, disclosure, or distribution is prohibited. If you 
are not the intended recipient, or an employee or agent responsible for 
delivering the message to the intended recipient, please contact the sender by 
reply e-mail and destroy all copies of the original message, including any 
attachments.
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] IHC for GAB1 and YAP1

[Cartun, Richard via Histonet]

Anyone out there doing IHC testing for GAB1 and YAP1 to subtype 
medulloblastoma?  Thank you.

Richard

Richard W. Cartun, MS, PhD
Director, Histology & The Martin M. Berman, MD Immunopathology & Morphologic 
Proteomics Laboratory
Director, Biospecimen Collection Programs
Assistant Director, Anatomic Pathology
Hartford Hospital
80 Seymour Street
Hartford, CT  06102
(860) 972-1596
(860) 545-2204 Fax


This e-mail message, including any attachments, is for the sole use of the 
intended recipient(s) and may contain confidential and privileged information. 
Any unauthorized review, use, disclosure, or distribution is prohibited. If you 
are not the intended recipient, or an employee or agent responsible for 
delivering the message to the intended recipient, please contact the sender by 
reply e-mail and destroy all copies of the original message, including any 
attachments.
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

Re: [Histonet] Return of Gross Only Specimens for Patient Use

[Cartun, Richard via Histonet]

I strongly discourage patients from taking their tissue out of the hospital.  
Once the specimen in placed in formalin it becomes a bio-hazard.  We will 
release certain specimens for burial; however, the patient (or family) must 
contact a funeral home or mortuary to pick-up the specimen, and they must sign 
a release.

Richard

Richard W. Cartun, MS, PhD
Director, Histology & The Martin M. Berman, MD Immunopathology & Morphologic 
Proteomics Laboratory
Director, Biospecimen Collection Programs
Assistant Director, Anatomic Pathology
Hartford Hospital
80 Seymour Street
Hartford, CT  06102
(860) 972-1596
(860) 545-2204 Fax



-Original Message-
From: ian bernard via Histonet [mailto:histonet@lists.utsouthwestern.edu]
Sent: Saturday, April 08, 2017 2:39 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Return of Gross Only Specimens for Patient Use

1.   Per a patient request, before returning an amputated digit (toe) to
a patient, we will remove the 10 % NBF from the tissue by washing the tissue
in running water for how long?



2.   As an alternative, we can have the provider submit the gross only
toe in saline and then relinquish the toe to the patient, explaining that 
ultimate degradation of the toe is inevitable unless fixed. Is there another 
safe fixative alternative that  can preserve the toe, say 100% alcohol and for 
how long?  The patient wants to keep his body parts for his burial.



3.   We will have the patient sign a  document explaining the exposure
risk to formalin fixed tissue as well as a release of medical records request 
form. Your thoughts?



4.   Is there any other validated procedure to remove formalin from
foreign bodies devices or tissue before release to the patient?



Note: The transaction above will take place 2-weeks after the case has been 
signed out and reported to the referring provider.  Your feedback?





v/r

IB



___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

This e-mail message, including any attachments, is for the sole use of the 
intended recipient(s) and may contain confidential and privileged information. 
Any unauthorized review, use, disclosure, or distribution is prohibited. If you 
are not the intended recipient, or an employee or agent responsible for 
delivering the message to the intended recipient, please contact the sender by 
reply e-mail and destroy all copies of the original message, including any 
attachments.

___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

Re: [Histonet] Immunoperoxidase Protocol on Cytospin

[Cartun, Richard via Histonet]

Since you only have 1 slide I would recommend following your regular protocol 
that you use for formalin-fixed, paraffin-embedded tissue.  I would use heat 
retrieval if that's what you use for FFPE tissue.  However, if you use enzyme 
digestion on FFPE tissue I would "not" digest your cytospin slide.  If you 
don't get any immunoreactivity it could be a "False Negative" due to 
pre-analytical variables.  I think it can be interesting to try experiments 
like this, but it's not the way to do IHC testing.

Richard

Richard W. Cartun, MS, PhD
Director, Histology & The Martin M. Berman, MD Immunopathology & Morphologic 
Proteomics Laboratory
Director, Biospecimen Collection Programs
Assistant Director, Anatomic Pathology
Hartford Hospital
80 Seymour Street
Hartford, CT  06102
(860) 972-1596
(860) 545-2204 Fax




-Original Message-
From: Margaryan, Naira [mailto:nmargar...@luriechildrens.org] 
Sent: Tuesday, March 14, 2017 3:24 PM
To: Cartun, Richard
Subject: RE: Immunoperoxidase Protocol on Cytospin

cytoplasmic staining for Nodal protein.

thanks
____________
From: Cartun, Richard [richard.car...@hhchealth.org]
Sent: Tuesday, March 14, 2017 2:13 PM
To: Margaryan, Naira; histonet-boun...@lists.utsouthwestern.edu
Subject: RE: Immunoperoxidase Protocol on Cytospin

What is the target?

Richard

Richard W. Cartun, MS, PhD
Director, Histology & The Martin M. Berman, MD Immunopathology & Morphologic 
Proteomics Laboratory Director, Biospecimen Collection Programs Assistant 
Director, Anatomic Pathology Hartford Hospital
80 Seymour Street
Hartford, CT  06102
(860) 972-1596
(860) 545-2204 Fax




-Original Message-
From: Margaryan, Naira via Histonet [mailto:histonet@lists.utsouthwestern.edu]
Sent: Tuesday, March 14, 2017 2:51 PM
To: histonet-boun...@lists.utsouthwestern.edu
Cc: histonet-request
Subject: [Histonet] Immunoperoxidase Protocol on Cytospin
Importance: High

Dear histonetters:

I never done ICC.

A scientist brought me a slide with Cytospined cells on for IHC.

I am going to fix with 10%NBF (it is only what I have now) then wash with TBST.

May I skip AR step?
May I use my usual IHC protocol and reagents which I usually use for FFPE 
tissue: blocks with H2O2, avidin/biotin/PB with TBST wash buffer

Thanks in advance,
Naira


Ranked nationally in all 10 pediatric specialties by U.S. News & World Report  
(LCHOC Ver 1.0)


This message contains confidential information and is intended only for the 
individual named. If you are not the named addressee you should not 
disseminate, distribute or copy this e-mail. Please notify the sender 
immediately by e-mail if you have received this e-mail by mistake and delete 
this e-mail from your system. E-mail transmission cannot be guaranteed to be 
secure or error-free as information could be intercepted, corrupted, lost, 
destroyed, arrive late or incomplete, or contain viruses. The sender therefore 
does not accept liability for any errors or omissions in the contents of this 
message, which arise as a result of e-mail transmission. If verification is 
required please request a hard-copy version.  (LCHOC VER 1.0)

___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

This e-mail message, including any attachments, is for the sole use of the 
intended recipient(s) and may contain confidential and privileged information. 
Any unauthorized review, use, disclosure, or distribution is prohibited. If you 
are not the intended recipient, or an employee or agent responsible for 
delivering the message to the intended recipient, please contact the sender by 
reply e-mail and destroy all copies of the original message, including any 
attachments.

___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] Serotonin

[Cartun, Richard via Histonet]

Can anyone recommend a good anti-Serotonin antibody for diagnostic morphologic 
proteomics testing (aka, Immunohistochemistry)?  Thank you.

Richard

Richard W. Cartun, MS, PhD
Director, Histology & The Martin M. Berman, MD Immunopathology & Morphologic 
Proteomics Laboratory
Director, Biospecimen Collection Programs
Assistant Director, Anatomic Pathology
Hartford Hospital
80 Seymour Street
Hartford, CT  06102
(860) 972-1596
(860) 545-2204 Fax


This e-mail message, including any attachments, is for the sole use of the 
intended recipient(s) and may contain confidential and privileged information. 
Any unauthorized review, use, disclosure, or distribution is prohibited. If you 
are not the intended recipient, or an employee or agent responsible for 
delivering the message to the intended recipient, please contact the sender by 
reply e-mail and destroy all copies of the original message, including any 
attachments.
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] BRG1 IHC Testing

[Cartun, Richard via Histonet]

Is anyone doing clinical IHC testing for BRG1/SMARCA4?  Thank you.

Richard

Richard W. Cartun, MS, PhD
Director, Histology & The Martin M. Berman, MD Immunopathology & Morphologic 
Proteomics Laboratory
Director, Biospecimen Collection Programs
Assistant Director, Anatomic Pathology
Hartford Hospital
80 Seymour Street
Hartford, CT  06102
(860) 972-1596
(860) 545-2204 Fax


This e-mail message, including any attachments, is for the sole use of the 
intended recipient(s) and may contain confidential and privileged information. 
Any unauthorized review, use, disclosure, or distribution is prohibited. If you 
are not the intended recipient, or an employee or agent responsible for 
delivering the message to the intended recipient, please contact the sender by 
reply e-mail and destroy all copies of the original message, including any 
attachments.
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] IHC for ALDH1

[Cartun, Richard via Histonet]

Is anyone doing IHC testing for Aldehyde Dehydrogenase 1 (ALDH1)?  Evidently, 
it is a prognostic maker used for breast CA.

Happy Holidays everyone!

Richard

Richard W. Cartun, MS, PhD
Director, Histology & The Martin M. Berman, MD Immunopathology & Morphologic 
Proteomics Laboratory
Director, Biospecimen Collection Programs
Assistant Director, Anatomic Pathology
Hartford Hospital
80 Seymour Street
Hartford, CT  06102
(860) 972-1596
(860) 545-2204 Fax


This e-mail message, including any attachments, is for the sole use of the 
intended recipient(s) and may contain confidential and privileged information. 
Any unauthorized review, use, disclosure, or distribution is prohibited. If you 
are not the intended recipient, or an employee or agent responsible for 
delivering the message to the intended recipient, please contact the sender by 
reply e-mail and destroy all copies of the original message, including any 
attachments.
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] Aluminum Testing

[Cartun, Richard via Histonet]

Has anyone ever heard of testing a tissue or body fluid sample for "accrued 
aluminum"?  We have a child who has had multiple immunizations and the parents 
wanted the specimen tested.  Unfortunately, none of this was communicated to us 
prior to the procedure being performed.  Thank you and "Happy Holidays" to 
everyone.

Richard

Richard W. Cartun, MS, PhD
Director, Histology & The Martin M. Berman, MD Immunopathology & Morphologic 
Proteomics Laboratory
Director, Biospecimen Collection Programs
Assistant Director, Anatomic Pathology
Hartford Hospital
80 Seymour Street
Hartford, CT  06102
(860) 972-1596
(860) 545-2204 Fax


This e-mail message, including any attachments, is for the sole use of the 
intended recipient(s) and may contain confidential and privileged information. 
Any unauthorized review, use, disclosure, or distribution is prohibited. If you 
are not the intended recipient, or an employee or agent responsible for 
delivering the message to the intended recipient, please contact the sender by 
reply e-mail and destroy all copies of the original message, including any 
attachments.
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] Hard copy pathology reports

[Cartun, Richard via Histonet]

Since we are now using EPIC we will no longer be providing (mailing) hard-copy 
pathology reports to our clients effective January 1, 2017.  Our healthcare 
providers now have access to all lab results and pathology reports in EPIC.  
Physicians can also be set-up for auto-faxing or can call client services for a 
faxed copy of the report if needed.  The issue we are having is with the 
providers that are "CC:'ed" on pathology requisitions.  Many of these 
individuals are not in our system or don't have access to EPIC.  Has anyone 
else experienced this issue and, if so, what have you done to make sure all 
care-givers get their pathology reports?  Thank you.

Richard

Richard W. Cartun, MS, PhD
Director, Histology & The Martin M. Berman, MD Immunopathology & Morphologic 
Proteomics Laboratory
Director, Biospecimen Collection Programs
Assistant Director, Anatomic Pathology
Hartford Hospital
80 Seymour Street
Hartford, CT  06102
(860) 972-1596
(860) 545-2204 Fax


This e-mail message, including any attachments, is for the sole use of the 
intended recipient(s) and may contain confidential and privileged information. 
Any unauthorized review, use, disclosure, or distribution is prohibited. If you 
are not the intended recipient, or an employee or agent responsible for 
delivering the message to the intended recipient, please contact the sender by 
reply e-mail and destroy all copies of the original message, including any 
attachments.
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

Re: [Histonet] ER/PR Uneven Staining

[Cartun, Richard via Histonet]

Can you provide more details about the uneven immunoreactivity?  Are the 
negative cells located at the periphery of the specimen or are the negative and 
positive tumor cells mixed together throughout the specimen?

Richard

Richard W. Cartun, MS, PhD
Director, Histology & The Martin M. Berman, MD Immunopathology & Morphologic 
Proteomics Laboratory
Director, Biospecimen Collection Programs
Assistant Director, Anatomic Pathology
Hartford Hospital
80 Seymour Street
Hartford, CT  06102
(860) 972-1596
(860) 545-2204 Fax

-Original Message-
From: Joanne Clark via Histonet [mailto:histonet@lists.utsouthwestern.edu]
Sent: Tuesday, November 29, 2016 4:09 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] ER/PR Uneven Staining

I had a breast needle core today that when stained with ER and PR the staining 
was uneven throughout the core, even though the cancer cells were present in 
the entire core.  The specimen had 10 hours fixation in 10% NBF.  I could 
understand the uneven staining from inadequate fixation on large grossed in 
breast tissue, but 10 hours with  needle core biopsies has always been more 
than sufficient.  Does anyone have any ideas?  We use Leica's ER and PR 
antibodies on the BOND.



Joanne Clark, BAAS, HT(ASCP)CM
Director of Histology

P.   (575) 622-5600
C.   (575) 317-6403
F.   (575) 622-3720
TF. (800) 753-7284

pcnm.com


Disclaimer: This electronic message may contain information that is 
Proprietary, Confidential, or legally privileged or protected. It is intended 
only for the use of the individual(s) and entity named in the message. If you 
are not an intended recipient of this message, please notify the sender 
immediately and delete the material from your computer. Do not deliver, 
distribute or copy this message and do not disclose its contents or take any 
action in reliance on the information it contains.
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

This e-mail message, including any attachments, is for the sole use of the 
intended recipient(s) and may contain confidential and privileged information. 
Any unauthorized review, use, disclosure, or distribution is prohibited. If you 
are not the intended recipient, or an employee or agent responsible for 
delivering the message to the intended recipient, please contact the sender by 
reply e-mail and destroy all copies of the original message, including any 
attachments.

___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

Re: [Histonet] processing cell blocks in formalin

[Cartun, Richard via Histonet]

Yes, I will send you the procedure that we use.

Richard

Richard W. Cartun, MS, PhD
Director, Histology & The Martin M. Berman, MD Immunopathology & Morphologic 
Proteomics Laboratory
Director, Biospecimen Collection Programs
Assistant Director, Anatomic Pathology
Hartford Hospital
80 Seymour Street
Hartford, CT  06102
(860) 972-1596
(860) 545-2204 Fax

-Original Message-
From: Algeo, Lacie A via Histonet [mailto:histonet@lists.utsouthwestern.edu]
Sent: Tuesday, November 01, 2016 3:10 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] processing cell blocks in formalin

Hi All,
Does anyone have a procedure for processing cellblocks in formalin that works 
well?
Thanks,
Lacie

Lacie Algeo, HTL (ASCP) MBCM
Histology Supervisor
Providence Sacred Heart Medical Center Laboratory
101 W 8th Avenue
L-2
Spokane, WA 99204
509-474-4418
FAX 509-474-2052
lacie.al...@providence.org


This message is intended for the sole use of the addressee, and may contain 
information that is priviledged, confidential and exempt from disclosure under 
applicable law.  If you are not the addressee, you are hereby notified that you 
may not use, copy, disclose or distribute to anyone the message or any 
information contained in the message.  If you have received this message in 
error, please immediately advise the sender by reply e-mail and delete this 
message.




This message is intended for the sole use of the addressee, and may contain 
information that is privileged, confidential and exempt from disclosure under 
applicable law. If you are not the addressee you are hereby notified that you 
may not use, copy, disclose, or distribute to anyone the message or any 
information contained in the message. If you have received this message in 
error, please immediately advise the sender by reply email and delete this 
message.
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

This e-mail message, including any attachments, is for the sole use of the 
intended recipient(s) and may contain confidential and privileged information. 
Any unauthorized review, use, disclosure, or distribution is prohibited. If you 
are not the intended recipient, or an employee or agent responsible for 
delivering the message to the intended recipient, please contact the sender by 
reply e-mail and destroy all copies of the original message, including any 
attachments.

___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

Re: [Histonet] Billing Questions

[Cartun, Richard via Histonet]

I can only speak to IHC billing.  My staff (now 2 people) in our IHC Lab does 
all the technical IHC billing manually.  We average about 3,500 billable IHC 
tests per month now.  The volume used to be higher, but our healthcare system 
sold most of our outreach business.

Richard

Richard W. Cartun, MS, PhD
Director, Histology & The Martin M. Berman, MD Immunopathology & Morphologic 
Proteomics Laboratory
Director, Biospecimen Collection Programs
Assistant Director, Anatomic Pathology
Hartford Hospital
80 Seymour Street
Hartford, CT  06102
(860) 972-1596
(860) 545-2204 Fax

-Original Message-
From: Gauch, Vicki via Histonet [mailto:histonet@lists.utsouthwestern.edu]
Sent: Wednesday, October 12, 2016 9:19 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Billing Questions

Hi,
 I've been asked to post a question regarding Pathology technical billing.  
With all of the changes that have been instituted over the past few years on 
the technical billing side in Pathology and the fact that our computer system 
cannot automate  it for us we have been manually billing  our IHC,etc. .  This 
is becoming a real problem since the supervisor and myself are the ones doing 
this and we are also working on the bench along with other duties.
  We are wondering who does that billing in your lab?  Do you have 
biller/coders ? Is it technical staff ?  If it is technical staff, what level  
?  Has anyone been able to get that billing automated?  Any info would be 
greatly appreciated.

Thank you,

Vicki Gauch
AMCH
Albany, NY


-
CONFIDENTIALITY NOTICE: This email and any attachments may contain confidential 
information that is protected by law and is for the sole use of the individuals 
or entities to which it is addressed. If you are not the intended recipient, 
please notify the sender by replying to this email and destroying all copies of 
the communication and attachments. Further use, disclosure, copying, 
distribution of, or reliance upon the contents of this email and attachments is 
strictly prohibited. To contact Albany Medical Center, or for a copy of our 
privacy practices, please visit us on the Internet at www.amc.edu.
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

This e-mail message, including any attachments, is for the sole use of the 
intended recipient(s) and may contain confidential and privileged information. 
Any unauthorized review, use, disclosure, or distribution is prohibited. If you 
are not the intended recipient, or an employee or agent responsible for 
delivering the message to the intended recipient, please contact the sender by 
reply e-mail and destroy all copies of the original message, including any 
attachments.

___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] Formalin in Physicians' Offices

[Cartun, Richard via Histonet]

Do physicians' offices need to have procedures/policies "on-site" regarding 
formalin if they put patient biopsy specimens into the small formalin 
containers?  What about formalin spill kits?  Thank you.

Richard

Richard W. Cartun, MS, PhD
Director, Histology & The Martin M. Berman, MD Immunopathology & Morphologic 
Proteomics Laboratory
Director, Biospecimen Collection Programs
Assistant Director, Anatomic Pathology
Hartford Hospital
80 Seymour Street
Hartford, CT  06102
(860) 972-1596
(860) 545-2204 Fax


This e-mail message, including any attachments, is for the sole use of the 
intended recipient(s) and may contain confidential and privileged information. 
Any unauthorized review, use, disclosure, or distribution is prohibited. If you 
are not the intended recipient, or an employee or agent responsible for 
delivering the message to the intended recipient, please contact the sender by 
reply e-mail and destroy all copies of the original message, including any 
attachments.
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

Re: [Histonet] Vacuolated and torn?

[Cartun, Richard via Histonet]

Microtomy is so important, but often over-looked.  You might try giving the 
paraffin block(s) to different people and have them all cut H, and then have 
your pathologist compare them.  If everyone's sections are suboptimal, then the 
problem is with fixation and/or processing.

Richard

Richard W. Cartun, MS, PhD
Director, Histology & The Martin M. Berman, MD Immunopathology & Morphologic 
Proteomics Laboratory
Director, Biospecimen Collection Programs
Assistant Director, Anatomic Pathology
Hartford Hospital
80 Seymour Street
Hartford, CT  06102
(860) 972-1596
(860) 545-2204 Fax



-Original Message-
From: Martin, Erin via Histonet [mailto:histonet@lists.utsouthwestern.edu]
Sent: Thursday, October 06, 2016 9:31 AM
To: histonet
Subject: [Histonet] Vacuolated and torn?

Good morning everyone!

One of my pathologists says that we are having a problem with the tissue on the 
slides looking vacuolated and torn.  He is convinced it is from microtomy.  
Anyone have any ideas?  I was thinking that it might be a processing issue.



Thanks in advance!

Erin Martin, Histology Supervisor
UCSF  Dermatopathology and Oral Pathology Service
1701 Divisadero St, San Francisco, CA 94044
415-353-7248

Confidentiality Notice
The information transmitted is intended only for the person or entity to which 
it is addressed and may contain confidential and/or privileged material.  Any 
review, retransmission, dissemination or other use of, or taking of any action 
in reliance upon, this information by persons or entities other than the 
intended recipient is prohibited.  If you receive this in error please contact 
the sender and delete the material from any computer.
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

This e-mail message, including any attachments, is for the sole use of the 
intended recipient(s) and may contain confidential and privileged information. 
Any unauthorized review, use, disclosure, or distribution is prohibited. If you 
are not the intended recipient, or an employee or agent responsible for 
delivering the message to the intended recipient, please contact the sender by 
reply e-mail and destroy all copies of the original message, including any 
attachments.

___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

Re: [Histonet] Kappa and Lambda ISH

[Cartun, Richard via Histonet]

Yes, the CAP now has a PT survey for HPV, EBV, and Kappa/Lambda in situ 
hybridization (ISH).

Richard

Richard W. Cartun, MS, PhD
Director, Histology & The Martin M. Berman, MD Immunopathology & Morphologic 
Proteomics Laboratory
Director, Biospecimen Collection Programs
Assistant Director, Anatomic Pathology
Hartford Hospital
80 Seymour Street
Hartford, CT  06102
(860) 972-1596
(860) 545-2204 Fax




-Original Message-
From: Vickroy, James via Histonet [mailto:histonet@lists.utsouthwestern.edu]
Sent: Wednesday, October 05, 2016 12:28 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Kappa and Lambda ISH


Our department does not perform IHC stains nor ISH Kappa and Lambda stains.  
Therefore we do not perform the CAP proficiency testing.  The pathologists 
participate in PIP testing and do not read ER, PR, or Her2 (predictive markers).
The pathologists do however interpret IHC stains and an occasional Kappa and 
Lambda stain.   Are there any other requirements that the pathologist or 
department needs to fulfil?   Again the patholgosits here are interpreting the 
IHC stains and reading Kappa and Lambda (the only ISH stains they interpret).

Also when I did work at an institution that performed Kappa and Lambda there 
was no CAP PT survey for Kappa and Lambda so we had to come up with an 
alternate PT assessment method.   Does CAP now have a Kappa and Lambda PT 
survey?

Your input is appreciated.

Thanks

Jim


Jim Vickroy
Histology Manager
Springfield Clinic, Main Campus, East Building
1025 South 6th Street
Springfield, Illinois  62703
Office:  217-528-7541, Ext. 15121
Email:  jvick...@springfieldclinic.com



This electronic message contains information from Springfield Clinic, LLP that 
may be confidential, privileged, and/or sensitive. This information is intended 
for the use of the individual(s) or entity(ies) named above. If you are not the 
intended recipient, be aware that disclosure, copying, distribution, or action 
taken on the contents of this information is strictly prohibited. If you have 
received this electronic message in error, please notify the sender 
immediately, by electronic mail, so that arrangements may be made for the 
retrieval of this electronic message. Thank you.
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

This e-mail message, including any attachments, is for the sole use of the 
intended recipient(s) and may contain confidential and privileged information. 
Any unauthorized review, use, disclosure, or distribution is prohibited. If you 
are not the intended recipient, or an employee or agent responsible for 
delivering the message to the intended recipient, please contact the sender by 
reply e-mail and destroy all copies of the original message, including any 
attachments.

___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

Re: [Histonet] CAP ANP.22970 Query

[Cartun, Richard via Histonet]

Do you participate in the CAP's PT program for ER/PR and HER2 IHC testing?  If 
so, you can have all your pathologists who interpret ER/PR/HER2 IHC testing 
score the PT TMAs, complete the worksheets, and then you can establish their 
interobserver variability.  If not, pull 20 cases where ER/PR/HER2 was 
performed, prepare a score-sheet and have all your pathologists interpret these 
20 cases.  Run the same comparison.  You will see ER positivity in the range of 
75-90%, PR positivity in the range of 60-75%, and HER2 should be between 
10-20%.  Obviously, these ranges will depend on your patient demographics, and 
the antibody clones and IHC detection used.

Richard

Richard W. Cartun, MS, PhD
Director, Histology & The Martin M. Berman, MD Immunopathology & Morphologic 
Proteomics Laboratory
Director, Biospecimen Collection Programs
Assistant Director, Anatomic Pathology
Hartford Hospital
80 Seymour Street
Hartford, CT  06102
(860) 972-1596
(860) 545-2204 Fax

-Original Message-
From: Joanne Clark via Histonet [mailto:histonet@lists.utsouthwestern.edu]
Sent: Monday, August 22, 2016 4:05 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] CAP ANP.22970 Query

Hi Histonetters, we are wondering what everyone else out there is doing to be 
compliant with the following requirement?  We do ER and PR by IHC  but dont 
know what published benchmarks are out there to compare ourselves to.  Also, 
how do you record interobserver variability amongst the pathologists?  Any 
insights into this would be appreciated.



ANP.22970 Annual Result Comparison Phase II For immunohistochemical and 
FISH/ISH tests that provide independent predictive information, the laboratory 
at least annually compares its patient results with published benchmarks, and 
evaluates interobserver variability among the pathologists in the laboratory.
NOTE: Individuals interpreting the assay must also have their concordance 
compared with each other and this concordance should also be at least 95%.
With specific reference to estrogen and progesterone receptor studies: in 
general, the overall proportion of ER-negative breast cancers (invasive and 
DCIS) should not exceed 30%. The proportion is somewhat lower in postmenopausal 
than premenopausal women (approximately 20% vs. 35%). The proportion is 
considerably lower in well-differentiated carcinomas (<10%) and certain special 
types of invasive carcinomas (<10% in lobular, tubular, and mucinous types).
The proportion of PgR-negative cases is 10-15% higher than for ER-negative in 
each of these settings. Investigation is warranted if the proportion of 
negative cases is significantly lower in any of these settings.

Joanne Clark, HT
Director of Histology
Pathology Consultants of New Mexico
Roswell, New Mexico




Disclaimer: This electronic message may contain information that is 
proprietary, confidential, or legally privileged or protected. It is intended 
only for the use of the individual(s) and entity named in the message. If you 
are not an intended recipient of this message, please notify the sender 
immediately and delete the material from your computer. Do not deliver, 
distribute or copy this message and do not disclose its contents or take any 
action in reliance on the information it contains.
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

This e-mail message, including any attachments, is for the sole use of the 
intended recipient(s) and may contain confidential and privileged information. 
Any unauthorized review, use, disclosure, or distribution is prohibited. If you 
are not the intended recipient, or an employee or agent responsible for 
delivering the message to the intended recipient, please contact the sender by 
reply e-mail and destroy all copies of the original message, including any 
attachments.

___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] Low temperature freezer

[Cartun, Richard via Histonet]

Our ancient "SoLow" -80 degree C. freezer died over the weekend.  What do you 
recommend as a replacement?  Do you prefer a chest vs. stand-up?  We use it 
primarily for tissue storage.  Thank you.

Richard

Richard W. Cartun, MS, PhD
Director, Histology & The Martin M. Berman, MD Immunopathology & Morphologic 
Proteomics Laboratory
Director, Biospecimen Collection Programs
Assistant Director, Anatomic Pathology
Hartford Hospital
80 Seymour Street
Hartford, CT  06102
(860) 972-1596
(860) 545-2204 Fax


This e-mail message, including any attachments, is for the sole use of the 
intended recipient(s) and may contain confidential and privileged information. 
Any unauthorized review, use, disclosure, or distribution is prohibited. If you 
are not the intended recipient, or an employee or agent responsible for 
delivering the message to the intended recipient, please contact the sender by 
reply e-mail and destroy all copies of the original message, including any 
attachments.
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] C4d IHC in myocardial biopsy

[Cartun, Richard via Histonet]

Are any of you an expert with C4d IHC in myocardial biopsy tissue?  We had a 
specimen this week that showed a pattern of immunoreactivity that I am not 
familiar with and I need to find out if this represents a specific disease 
process.  Thank you.

Richard

Richard W. Cartun, MS, PhD
Director, Histology & The Martin M. Berman, MD Immunopathology & Morphologic 
Proteomics Laboratory
Director, Biospecimen Collection Programs
Assistant Director, Anatomic Pathology
Hartford Hospital
80 Seymour Street
Hartford, CT  06102
(860) 972-1596
(860) 545-2204 Fax

This e-mail message, including any attachments, is for the sole use of the 
intended recipient(s) and may contain confidential and privileged information. 
Any unauthorized review, use, disclosure, or distribution is prohibited. If you 
are not the intended recipient, or an employee or agent responsible for 
delivering the message to the intended recipient, please contact the sender by 
reply e-mail and destroy all copies of the original message, including any 
attachments.
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] Testicular biopsy for infertility

[Cartun, Richard via Histonet]

What are people fixing testicular biopsies in to evaluate infertility?  In the 
past, I believe fixatives such as Zenker's and Bouin's were used for this 
purpose since they enhance nuclear detail.  Obviously, those fixatives can no 
longer be used.  Thank you.

Richard

Richard W. Cartun, MS, PhD
Director, Histology & The Martin M. Berman, MD Immunopathology & Morphologic 
Proteomics Laboratory
Director, Biospecimen Collection Programs
Assistant Director, Anatomic Pathology
Hartford Hospital
80 Seymour Street
Hartford, CT  06102
(860) 972-1596
(860) 545-2204 Fax


This e-mail message, including any attachments, is for the sole use of the 
intended recipient(s) and may contain confidential and privileged information. 
Any unauthorized review, use, disclosure, or distribution is prohibited. If you 
are not the intended recipient, or an employee or agent responsible for 
delivering the message to the intended recipient, please contact the sender by 
reply e-mail and destroy all copies of the original message, including any 
attachments.
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

Re: [Histonet] P16

[Cartun, Richard via Histonet]

We purchase the antibody from Ventana and run it on our Bond Max platforms.  
During re-optimization, we found we could dilute it 1:10 (15 minutes) following 
"H2" (high pH) retrieval and still get excellent results.

Richard

Richard W. Cartun, MS, PhD
Director, Histology and the Martin M. Berman, MD Immunopathology and 
Morphologic Proteomics Laboratory
Director, Biospecimen Collection Programs
Assistant Director, Anatomic Pathology
Department of Pathology and Laboratory Medicine
Hartford Hospital
80 Seymour Street
Hartford, CT  06102
(860) 972-1596 Office
(860) 545-2204 Fax


From: Dessoye, Michael via Histonet [histonet@lists.utsouthwestern.edu]
Sent: Wednesday, June 08, 2016 1:14 PM
To: Paula; histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] P16

Ventana/Roche bought out MTM which had the patent, so they're the only vendor 
you can get it from.  Since you're using Bond you obviously don't want it in a 
dispenser, but you can buy their manual staining kit and just use the antibody 
from that.

Michael J. Dessoye, M.S. | Histology/Toxicology/RIA Supervisor | Commonwealth 
Health Laboratory Services | mjdess...@commonwealthhealth.net | 575 N. River 
Street | Wilkes Barre, PA 18764 | Tel: 570-552-1432 | Fax: 570-552-1484


-Original Message-
From: Paula [mailto:plu...@biopath.org]
Sent: Wednesday, June 08, 2016 12:14 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] P16

Hello,

Our pathologist would like us to get the P16 antibody. We have the Bond III and 
we use ready to use antibodies. Leica doesn't carry this antibody and so I was 
hoping someone can tell me which vendor carries it and that I can order it from.

Thanks in advance for your help

Paula

Lab manager for BioPath Medical Group in California




--
Disclaimer: This electronic message may contain information that is 
Proprietary, Confidential, or legally privileged or protected. It is intended 
only for the use of the individual(s) and entity named in the message. If you 
are not an intended recipient of this message, please notify the sender 
immediately and delete the material from your computer. Do not deliver, 
distribute or copy this message and do not disclose its contents or take any 
action in reliance on the information it contains.
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

This e-mail message, including any attachments, is for the sole use of the 
intended recipient(s) and may contain confidential and privileged information. 
Any unauthorized review, use, disclosure, or distribution is prohibited. If you 
are not the intended recipient, or an employee or agent responsible for 
delivering the message to the intended recipient, please contact the sender by 
reply e-mail and destroy all copies of the original message, including any 
attachments.

___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] FW: Question - CoPath

[Cartun, Richard via Histonet]

Make that "Sunquest" CoPath ..

Richard

-Original Message-----
From: Cartun, Richard via Histonet [mailto:histonet@lists.utsouthwestern.edu] 
Sent: Tuesday, May 24, 2016 4:09 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Question - CoPath

We will be switching from Cerner CoPath to Sunrise CoPath in the near future 
(part of our Epic conversion).  Is there anyone using Sunrise CoPath that uses 
a prostate diagram in their report to demonstrate the absence or presence of 
cancer in the different quadrants?  Thank you.

Richard

Richard W. Cartun, MS, PhD
Director, Histology & The Martin M. Berman, MD Immunopathology & Morphologic 
Proteomics Laboratory Director, Biospecimen Collection Programs Assistant 
Director, Anatomic Pathology Hartford Hospital
80 Seymour Street
Hartford, CT  06102
(860) 972-1596
(860) 545-2204 Fax


This e-mail message, including any attachments, is for the sole use of the 
intended recipient(s) and may contain confidential and privileged information. 
Any unauthorized review, use, disclosure, or distribution is prohibited. If you 
are not the intended recipient, or an employee or agent responsible for 
delivering the message to the intended recipient, please contact the sender by 
reply e-mail and destroy all copies of the original message, including any 
attachments.
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] Question - CoPath

[Cartun, Richard via Histonet]

We will be switching from Cerner CoPath to Sunrise CoPath in the near future 
(part of our Epic conversion).  Is there anyone using Sunrise CoPath that uses 
a prostate diagram in their report to demonstrate the absence or presence of 
cancer in the different quadrants?  Thank you.

Richard

Richard W. Cartun, MS, PhD
Director, Histology & The Martin M. Berman, MD Immunopathology & Morphologic 
Proteomics Laboratory
Director, Biospecimen Collection Programs
Assistant Director, Anatomic Pathology
Hartford Hospital
80 Seymour Street
Hartford, CT  06102
(860) 972-1596
(860) 545-2204 Fax


This e-mail message, including any attachments, is for the sole use of the 
intended recipient(s) and may contain confidential and privileged information. 
Any unauthorized review, use, disclosure, or distribution is prohibited. If you 
are not the intended recipient, or an employee or agent responsible for 
delivering the message to the intended recipient, please contact the sender by 
reply e-mail and destroy all copies of the original message, including any 
attachments.
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] Date-of-Service for Consults

[Cartun, Richard via Histonet]

I have always maintained that the "Date-of-Service" for a pathology consult is 
the date that the pathology consult is initiated or requested.  Is that correct?

Richard

Richard W. Cartun, MS, PhD
Director, Histology & The Martin M. Berman, MD Immunopathology & Morphologic 
Proteomics Laboratory
Director, Biospecimen Collection Programs
Assistant Director, Anatomic Pathology
Hartford Hospital
80 Seymour Street
Hartford, CT  06102
(860) 972-1596
(860) 545-2204 Fax


This e-mail message, including any attachments, is for the sole use of the 
intended recipient(s) and may contain confidential and privileged information. 
Any unauthorized review, use, disclosure, or distribution is prohibited. If you 
are not the intended recipient, or an employee or agent responsible for 
delivering the message to the intended recipient, please contact the sender by 
reply e-mail and destroy all copies of the original message, including any 
attachments.
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

Re: [Histonet] GLOMERULI ADEQUACY

[Cartun, Richard via Histonet]

We provide on-site assessment of the renal tissue obtained in Ultrasound, both 
at our main hospital and at our Children's hospital across the street.  I 
handle probably 95% of the biopsies; I have two pathology colleagues (MDs) that 
handle the other 5% when I am not here.  Occasionally, a resident or fellow 
(trained by me) will handle a biopsy after-hours.  We place the core(s) on a 
glass slide with a few drops of saline or RPMI and then examine it under a 
microscope using 4x and 10x objectives in Ultrasound.  We tell the 
interventional radiologist/PA or the nephrologist performing the procedure when 
the specimen is adequate.  We usually obtain adequate tissue with 2, 18-gauge 
needle cores in more than 90% of the procedures; sometimes we need to ask for a 
3rd core.  On the other hand, I can make one core work when it's more than 2.0 
cm long if there's cortex at both ends.  However, I don't recommend obtaining 
cores this long.  We have not had to repeat a biopsy over the last 25 years
 .

Richard

Richard W. Cartun, MS, PhD
Director, Histology & The Martin M. Berman, MD Immunopathology & Morphologic 
Proteomics Laboratory
Director, Biospecimen Collection Programs
Assistant Director, Anatomic Pathology
Hartford Hospital
80 Seymour Street
Hartford, CT  06102
(860) 972-1596
(860) 545-2204 Fax

-Original Message-
From: Melissa Likens via Histonet [mailto:histonet@lists.utsouthwestern.edu]
Sent: Wednesday, April 13, 2016 10:22 AM
To: 'histonet@lists.utsouthwestern.edu'
Subject: [Histonet] GLOMERULI ADEQUACY

I have a question about how other institutions handle microscopic evaluation of 
glomeruli adequacy in renal specimens?  Specifically, who at you looks at the 
cores to determine if glomeruli are present before submitting specimens for 
further testing?  Do the pathologists look at them? Radiologists performing the 
cores?  Other staff?
Also, any links or recommendations for training for evaluating renal biopsies 
for glomeruli would be appreciated.
Thanks, Melissa
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

This e-mail message, including any attachments, is for the sole use of the 
intended recipient(s) and may contain confidential and privileged information. 
Any unauthorized review, use, disclosure, or distribution is prohibited. If you 
are not the intended recipient, or an employee or agent responsible for 
delivering the message to the intended recipient, please contact the sender by 
reply e-mail and destroy all copies of the original message, including any 
attachments.

___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

Re: [Histonet] Validating IHC on decalcified specimens

[Cartun, Richard via Histonet]

I have a validation file for ER, PR, and HER2 IHC testing on decalcified bone 
specimens with metastatic breast cancer.  I compared the results with other 
specimens from the same patient (usually the primary breast cancer) that were 
not decalcified.

Richard

Richard W. Cartun, MS, PhD
Director, Histology & The Martin M. Berman, MD Immunopathology & Morphologic 
Proteomics Laboratory
Director, Biospecimen Collection Programs
Assistant Director, Anatomic Pathology
Hartford Hospital
80 Seymour Street
Hartford, CT  06102
(860) 972-1596
(860) 545-2204 Fax

-Original Message-
From: Teri Johnson via Histonet [mailto:histonet@lists.utsouthwestern.edu]
Sent: Tuesday, April 05, 2016 2:50 PM
To: 'histonet@lists.utsouthwestern.edu'
Subject: [Histonet] Validating IHC on decalcified specimens

Hi Histonetters,

Can someone tell me if they do anything specific to validate their IHC markers 
on decalcified specimens? If no, do you test them anyway?

Teri Johnson
Manager, Clinical Trial Testing
Genoptix, Inc., a Novartis company
BioPharma
1811 Aston Avenue
Carlsbad, CA  92008
USA

Phone +1 760 516 5954
tejohn...@genoptix.com
www.genoptix.com




CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for 
the sole use of the intended recipient(s) and contains information that is 
confidential and proprietary to Genoptix Medical Laboratory or its 
subsidiaries. Any unauthorized review, use, disclosure or distribution is 
prohibited. If you are not the intended recipient, immediately contact the 
sender by e-mail and destroy all copies of the original message.
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

This e-mail message, including any attachments, is for the sole use of the 
intended recipient(s) and may contain confidential and privileged information. 
Any unauthorized review, use, disclosure, or distribution is prohibited. If you 
are not the intended recipient, or an employee or agent responsible for 
delivering the message to the intended recipient, please contact the sender by 
reply e-mail and destroy all copies of the original message, including any 
attachments.

___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

Re: [Histonet] tests on old cases

[Cartun, Richard via Histonet]

If the request is received more than "30" days from the original 
date-of-service, we re-accession the specimen, and bill and report the test 
result(s) under the new accession number.  The requesting MD now becomes the 
submitting MD.

Richard

Richard W. Cartun, MS, PhD
Director, Histology & The Martin M. Berman, MD Immunopathology & Morphologic 
Proteomics Laboratory
Director, Biospecimen Collection Programs
Assistant Director, Anatomic Pathology
Hartford Hospital
80 Seymour Street
Hartford, CT  06102
(860) 972-1596
(860) 545-2204 Fax

 
-Original Message-
From: Noelle Linke via Histonet [mailto:histonet@lists.utsouthwestern.edu] 
Sent: Friday, April 01, 2016 11:42 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] tests on old cases

Hi all,

We have been getting a lot of requests for tests such as Lynch syndrome on old 
hospital cases.  How do all of you handle the billing on these?  Do you ask the 
clinician to send updated insurance information and then log in a new case?

Thank you,
Noëlle

Noëlle Linke, MS, HTL(ASCP) QIHC
Manager, Anatomic Pathology
Pacific Diagnostic Laboratories
nli...@sbch.org
Phone: (805) 324-9814
Fax: (805) 696-6433






CH Disclaimer: This electronic mail message is intended exclusively for the 
individual or entity to which it is addressed. This message, together with any 
attachment, may contain confidential and privileged information. Any views, 
opinions or conclusions expressed in this message are those of the individual 
sender and do not necessarily reflect the views of Cottage Health, its 
subsidiaries or affiliates. This document may also contain information covered 
under the Health Insurance Portability and Accountability Act (HIPAA, PL 
104-191) and implementing regulations and must be protected in accordance with 
those provisions. Re-disclosure without patient consent or as otherwise 
permitted by law is prohibited. Any unauthorized review, retransmission, use, 
printing, copying, retention, disclosure, distribution or the taking of any 
action in reliance upon this information by persons or entities other than the 
intended recipient is strictly prohibited and may be unlawful. If you have 
received this message in error, please immediately advise the sender by reply 
email message to the sender and delete all copies of this message from your 
system without copying.


___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] Slide scribe

[Cartun, Richard via Histonet]

Years ago, I obtained a metal scribe for etching glass slides, but I can't 
remember which Histology company I got it from.  Any suggestions?  Thank you.

Richard

Richard W. Cartun, MS, PhD
Director, Histology & The Martin M. Berman, MD Immunopathology & Morphologic 
Proteomics Laboratory
Director, Biospecimen Collection Programs
Assistant Director, Anatomic Pathology
Hartford Hospital
80 Seymour Street
Hartford, CT  06102
(860) 972-1596
(860) 545-2204 Fax


This e-mail message, including any attachments, is for the sole use of the 
intended recipient(s) and may contain confidential and privileged information. 
Any unauthorized review, use, disclosure, or distribution is prohibited. If you 
are not the intended recipient, or an employee or agent responsible for 
delivering the message to the intended recipient, please contact the sender by 
reply e-mail and destroy all copies of the original message, including any 
attachments.
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] IgG4

[Cartun, Richard via Histonet]

I am curious to hear which clone people are using to detect IgG4-positive 
plasma cells in FFPE tissue.  Thank you.

Richard

Richard W. Cartun, MS, PhD
Director, Histology & The Martin M. Berman, MD Immunopathology & Morphologic 
Proteomics Laboratory
Director, Biospecimen Collection Programs
Assistant Director, Anatomic Pathology
Hartford Hospital
80 Seymour Street
Hartford, CT  06102
(860) 972-1596
(860) 545-2204 Fax


This e-mail message, including any attachments, is for the sole use of the 
intended recipient(s) and may contain confidential and privileged information. 
Any unauthorized review, use, disclosure, or distribution is prohibited. If you 
are not the intended recipient, or an employee or agent responsible for 
delivering the message to the intended recipient, please contact the sender by 
reply e-mail and destroy all copies of the original message, including any 
attachments.
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] IgG4

[Cartun, Richard via Histonet]

Hi everyone.  Which antibody (clone) are you using for the immunohistochemical 
detection of IgG4 in FFPE tissue?  I just found out that the clone that we were 
using is no longer available.  Thank you!

Richard

Richard W. Cartun, MS, PhD
Director, Histology & The Martin M. Berman, MD Immunopathology & Morphologic 
Proteomics Laboratory
Director, Biospecimen Collection Programs
Assistant Director, Anatomic Pathology
Hartford Hospital
80 Seymour Street
Hartford, CT  06102
(860) 972-1596
(860) 545-2204 Fax


This e-mail message, including any attachments, is for the sole use of the 
intended recipient(s) and may contain confidential and privileged information. 
Any unauthorized review, use, disclosure, or distribution is prohibited. If you 
are not the intended recipient, or an employee or agent responsible for 
delivering the message to the intended recipient, please contact the sender by 
reply e-mail and destroy all copies of the original message, including any 
attachments.
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] Sunquest CoPath

[Cartun, Richard via Histonet]

Dear Histology Colleagues:

I need your help once again.  Our healthcare system is in the process of 
switching over to Sunquest CoPath; we currently have Cerner CoPath now.  I have 
been told that when using their bar code and tracking system one cannot 
generate unstained slides (for possible histochemical stains and/or IHC 
testing) upfront (at the time that the H are cut).  Evidently, this is 
because the unique identifier for that unstained slide cannot be modified for 
ancillary testing.  Is this true?  I cannot believe that a software program for 
pathology laboratories in this day and age would not allow for this.  Is anyone 
using this system and have you found a solution to this problem?  We have a lot 
of slide protocols that include the cutting of unstained slides upfront.  I 
apologize if I have incorrectly stated the facts here; however, this is how it 
was explained to me in a conference call earlier this week.

As always, thank you for sharing your knowledge and experiences.

Richard

Richard W. Cartun, MS, PhD
Director, Histology & The Martin M. Berman, MD Immunopathology & Morphologic 
Proteomics Laboratory
Director, Biospecimen Collection Programs
Assistant Director, Anatomic Pathology
Hartford Hospital
80 Seymour Street
Hartford, CT  06102
(860) 972-1596
(860) 545-2204 Fax


This e-mail message, including any attachments, is for the sole use of the 
intended recipient(s) and may contain confidential and privileged information. 
Any unauthorized review, use, disclosure, or distribution is prohibited. If you 
are not the intended recipient, or an employee or agent responsible for 
delivering the message to the intended recipient, please contact the sender by 
reply e-mail and destroy all copies of the original message, including any 
attachments.
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] Shipping paraffin blocks overseas

[Cartun, Richard via Histonet]

It's been a long time since I have sent a paraffin tissue block out of the 
country.  Can someone bring me up-to-date on requirements for shipping paraffin 
tissue blocks overseas (specifically Germany).  I believe FedEx will be used 
for the shipping.  Thank you!

Richard

Richard W. Cartun, MS, PhD
Director, Histology & The Martin M. Berman, MD Immunopathology & Morphologic 
Proteomics Laboratory
Director, Biospecimen Collection Programs
Assistant Director, Anatomic Pathology
Hartford Hospital
80 Seymour Street
Hartford, CT  06102
(860) 972-1596
(860) 545-2204 Fax


This e-mail message, including any attachments, is for the sole use of the 
intended recipient(s) and may contain confidential and privileged information. 
Any unauthorized review, use, disclosure, or distribution is prohibited. If you 
are not the intended recipient, or an employee or agent responsible for 
delivering the message to the intended recipient, please contact the sender by 
reply e-mail and destroy all copies of the original message, including any 
attachments.
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] H. pylori control tissue

[Cartun, Richard via Histonet]

Nancy Schmitt from Dubuque, IA asked about control tissue for H. pylori.  I 
have tried e-mailing her, but my e-mail was returned as "Undeliverable".  
Nancy, please contact me.

Richard

Richard W. Cartun, MS, PhD
Director, Histology & The Martin M. Berman, MD Immunopathology & Morphologic 
Proteomics Laboratory
Director, Biospecimen Collection Programs
Assistant Director, Anatomic Pathology
Hartford Hospital
80 Seymour Street
Hartford, CT  06102
(860) 972-1596
(860) 545-2204 Fax


This e-mail message, including any attachments, is for the sole use of the 
intended recipient(s) and may contain confidential and privileged information. 
Any unauthorized review, use, disclosure, or distribution is prohibited. If you 
are not the intended recipient, or an employee or agent responsible for 
delivering the message to the intended recipient, please contact the sender by 
reply e-mail and destroy all copies of the original message, including any 
attachments.
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] Patient identifiers

[Cartun, Richard via Histonet]

Thanks to all who responded to my question on patient identifiers for prostate 
biopsies.

Richard

Richard W. Cartun, MS, PhD
Director, Histology & The Martin M. Berman, MD Immunopathology & Morphologic 
Proteomics Laboratory
Director, Biospecimen Collection Programs
Assistant Director, Anatomic Pathology
Hartford Hospital
80 Seymour Street
Hartford, CT  06102
(860) 972-1596
(860) 545-2204 Fax


This e-mail message, including any attachments, is for the sole use of the 
intended recipient(s) and may contain confidential and privileged information. 
Any unauthorized review, use, disclosure, or distribution is prohibited. If you 
are not the intended recipient, or an employee or agent responsible for 
delivering the message to the intended recipient, please contact the sender by 
reply e-mail and destroy all copies of the original message, including any 
attachments.
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] Patient identifiers

[Cartun, Richard via Histonet]

What are people accepting for the "two" identifiers on the small formalin 
bottles that are used for prostate biopsy specimens?  Can you accept the 
patient's initials in place of the full name?  Thank you.

Richard

Richard W. Cartun, MS, PhD
Director, Histology & The Martin M. Berman, MD Immunopathology & Morphologic 
Proteomics Laboratory
Director, Biospecimen Collection Programs
Assistant Director, Anatomic Pathology
Hartford Hospital
80 Seymour Street
Hartford, CT  06102
(860) 972-1596
(860) 545-2204 Fax


This e-mail message, including any attachments, is for the sole use of the 
intended recipient(s) and may contain confidential and privileged information. 
Any unauthorized review, use, disclosure, or distribution is prohibited. If you 
are not the intended recipient, or an employee or agent responsible for 
delivering the message to the intended recipient, please contact the sender by 
reply e-mail and destroy all copies of the original message, including any 
attachments.
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

Re: [Histonet] Histology Workload

[Cartun, Richard via Histonet]

Dear Amy:

Some of the companies doing molecular testing will pay you a fee for "specimen 
procurement/handling" since they realize the burden that these requests put on 
us.  I suggest calling the company(s) that you deal with and see if you can 
work something out with them.  And, you're absolutely correct; we are being 
asked to do more work on specimens today, yet reimbursements are going down.  
Not a good combination.  Based on your description of the work that you and 
your colleagues are doing, you have a good case for additional staffing.  It 
looks like you would benefit from a lab assistant that could help with 
accessioning, billing, and send-outs.

Richard

Richard W. Cartun, MS, PhD
Director, Histology & The Martin M. Berman, MD Immunopathology & Morphologic 
Proteomics Laboratory
Director, Biospecimen Collection Programs
Assistant Director, Anatomic Pathology
Hartford Hospital
80 Seymour Street
Hartford, CT  06102
(860) 972-1596
(860) 545-2204 Fax

-Original Message-
From: Amy Self via Histonet [mailto:histonet@lists.utsouthwestern.edu]
Sent: Monday, August 31, 2015 4:41 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Histology Workload

Happy Monday to Everyone,

I have been trying[ to figure out how to justify additional help in histology - 
and it's been hard.  My facility staffs according to billables.  This by no 
means feels fair to anyone in this department.  You could have a specimen that 
will provide one billable CPT code but can produce as many as 20 paraffin 
blocks.  And now it seems like we are getting many request for molecular test 
that require us to mail the patients material out but we have no way of showing 
that we did this work - we call this free work. We get no credit for time spent 
preparing and packaging pathology material to send out to reference labs. Am I 
missing any CPT codes that can be used to show that we in fact did something in 
addition to routine pathology to this case.

We have 1 histotech - 1 histotech/histology supervisor and one histology 
assistant.
Our block load averages from 125 to 170 daily.
We also prep non-gyn cytologies.
Accession all specimens that come in that lab.
All mail-outs
The histotech/histology supervisor is responsible for all of the billing as 
well as keeping up with new and old policies/ requirements for CAP/ auditing 
billing and the list could go on.

We are drowning in our own workload but don't know how to prove that the help 
is needed.  Any help - advice - suggestions anything will be appreciated. How 
can I prove to upper management that we need more help although the 
billables/productivity numbers say different?

Thanks in advance,
Amy Self
Histology Lab Senior Tech
Lab
Tidelands Georgetown Memorial Hospital
606 Black River Road
Georgetown, SC 29440
843-520-8711
as...@tidelandshealth.org

NOTE:
 The information contained in this message may be privileged, confidential and 
protected from disclosure. If the reader of this message is not the intended 
recipient, or an employee or agent responsible for delivering this message to 
the intended recipient, you are hereby notified that any dissemination, 
distribution or copying of this communication is strictly prohibited. If you 
have received this communication in error, please notify us immediately by 
replying to this message and deleting it from your computer.
Thank you.
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

This e-mail message, including any attachments, is for the sole use of the 
intended recipient(s) and may contain confidential and privileged information. 
Any unauthorized review, use, disclosure, or distribution is prohibited. If you 
are not the intended recipient, or an employee or agent responsible for 
delivering the message to the intended recipient, please contact the sender by 
reply e-mail and destroy all copies of the original message, including any 
attachments.

___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] IHC Weekend Coverage

[Cartun, Richard via Histonet]

How many of you working in hospital-based pathology laboratories run IHC on 
weekends?  Thank you.

Richard

Richard W. Cartun, MS, PhD
Director, Histology  Immunopathology
Director, Biospecimen Collection Programs
Assistant Director, Anatomic Pathology
Hartford Hospital
80 Seymour Street
Hartford, CT  06102
(860) 972-1596
(860) 545-2204 Fax


This e-mail message, including any attachments, is for the sole use of the 
intended recipient(s) and may contain confidential and privileged information. 
Any unauthorized review, use, disclosure, or distribution is prohibited. If you 
are not the intended recipient, or an employee or agent responsible for 
delivering the message to the intended recipient, please contact the sender by 
reply e-mail and destroy all copies of the original message, including any 
attachments.
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

Re: [Histonet] Breast fixation

[Cartun, Richard via Histonet]

There are several studies (one listed below) that have addressed the issue of 
fixation past the recommended 72 hour maximum.  In my opinion, the science 
behind these fixation recommendations is weak at best.  In my own experience, 
I have seen no problem whatsoever going well past 72 hours of fixation.  The 
majority of breast cases will show internal positive controls for ER and PR to 
validate that the tissue is satisfactory for testing.  Remember, working with 
your pathologist(s) you can perform your own validation on-site for extended 
fixation time.  The real culprit for poor ER, PR, and HER2 IHC results is 
inadequate fixation.

Reference:  Tong LC, Nelson N, Tsourigiannis J, et al.:  The effect of 
prolonged fixation on the IHC evaluation of ER, PR, and HER2 expression in 
invasive breast cancer:  A prospective study.  Am J Surg Pathol 2011;35:545-552.

Richard

Richard W. Cartun, MS, PhD
Director, Histology  Immunopathology
Director, Biospecimen Collection Programs
Assistant Director, Anatomic Pathology
Hartford Hospital
80 Seymour Street
Hartford, CT  06102
(860) 972-1596
(860) 545-2204 Fax

-Original Message-
From: Heckford, Karen - SMMC-SF via Histonet 
[mailto:histonet@lists.utsouthwestern.edu]
Sent: Wednesday, July 29, 2015 11:02 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Breast fixation

Good Morning,
I have a question about breast fixation.   I am in a little bit of a pickle 
with the 6-72 hour rule for the fixation on breast tissue.   Friday I am 
getting 2 breast cases in the afternoon and both will not have the required 
minimum 6 hour formalin fixation for my per diem to cut early Saturday morning. 
  He will not be able to make it in again until Monday night.  The tissue will 
be about 3-4 hours (this includes time on the processor)  over the 72 hour 
maximum.Does anyone have any suggestions on what can be done?  We are a one 
person show here.

Thanks,

Karen Heckford HT ASCP CE
Lead Histology Technician
St. Mary's Medical Center
450 Stanyan St.
San Francisco, Ca. 94117
415-668-1000 ext. 6167
karen.heckf...@dignityhealth.org

  Caution:  This email message, including all content and attachments, is 
CONFIDENTIAL and may be of a nature that is LEGALLY PRIVILEGED.  The 
information contained in this email message is intended only for the use of the 
recipient(s) named above. If the reader of this message is not the intended 
recipient or an agent responsible for delivering it to the intended recipient, 
you have received this document in error.  Any further review, dissemination, 
distribution, or copying of this message is strictly prohibited.  If you have 
received this communication in error, please notify us  immediately by reply 
email.  Thank you.



___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

This e-mail message, including any attachments, is for the sole use of the 
intended recipient(s) and may contain confidential and privileged information. 
Any unauthorized review, use, disclosure, or distribution is prohibited. If you 
are not the intended recipient, or an employee or agent responsible for 
delivering the message to the intended recipient, please contact the sender by 
reply e-mail and destroy all copies of the original message, including any 
attachments.

___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

Re: [Histonet] CMV antibody clone

[Cartun, Richard]

We use DAKO's (an Agilent Technologies Company) product number M0854 (clones 
DDG9  CCH2).

Richard
Richard W. Cartun, MS, PhD
Director, Histology  Immunopathology
Director, Biospecimen Collection Programs
Assistant Director, Anatomic Pathology
Hartford Hospital
80 Seymour Street
Hartford, CT  06102
(860) 972-1596
(860) 545-2204 Fax



-Original Message-
From: Cooper, Brian [mailto:bcoo...@chla.usc.edu]
Sent: Monday, July 13, 2015 3:51 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] CMV antibody clone

Good afternoon Histonet,

Just taking a quick poll to find out which CMV clone most folks are running in 
their labs.  Your feedback is much appreciated.

Thanks,

Brian D. Cooper, HT (ASCP)CM | Histology Supervisor Department of Pathology and 
Laboratory Medicine Children's Hospital Los Angeles
4650 Sunset Blvd MS#43- Los Angeles, CA 90027
Ph: 323.361.3357
bcoo...@chla.usc.edumailto:bcoo...@chla.usc.edu



-
CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for 
the sole use of the intended recipient(s) and may contain confidential or 
legally privileged information. Any unauthorized review, use, disclosure or 
distribution is prohibited. If you are not the intended recipient, please 
contact the sender by reply e-mail and destroy all copies of this original 
message.

-

___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

This e-mail message, including any attachments, is for the sole use of the 
intended recipient(s) and may contain confidential and privileged information. 
Any unauthorized review, use, disclosure, or distribution is prohibited. If you 
are not the intended recipient, or an employee or agent responsible for 
delivering the message to the intended recipient, please contact the sender by 
reply e-mail and destroy all copies of the original message, including any 
attachments.

___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

Re: [Histonet] Ventana antibody - H pylori

[Cartun, Richard]

Is the Ventana antibody polyclonal or monoclonal?

Richard

Richard W. Cartun, MS, PhD
Director, Histology  Immunopathology
Director, Biospecimen Collection Programs
Assistant Director, Anatomic Pathology
Hartford Hospital
80 Seymour Street
Hartford, CT  06102
(860) 972-1596
(860) 545-2204 Fax

-Original Message-
From: Histology Technician [mailto:histology81...@att.net]
Sent: Thursday, July 09, 2015 4:51 PM
To: Histonet; histonet-requ...@lists.utsouthwestern.edu
Subject: [Histonet] Ventana antibody - H pylori

 Does anyone else have constant problems with the Ventana antibody H pylori 
having a dirty background stain?  My pathologists are constantly complaining 
about it and when I call CS I get the same response...decon the instrument, try 
a new lot number, etc... it's never truly resolved.  Any thoughts?  Thanks!
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

This e-mail message, including any attachments, is for the sole use of the 
intended recipient(s) and may contain confidential and privileged information. 
Any unauthorized review, use, disclosure, or distribution is prohibited. If you 
are not the intended recipient, or an employee or agent responsible for 
delivering the message to the intended recipient, please contact the sender by 
reply e-mail and destroy all copies of the original message, including any 
attachments.
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

Re: [Histonet] IHC turnaround time

[Cartun, Richard]

I direct a busy and very efficient (I think) IHC lab staffed with 2.875 FTEs 
who spend most of their time doing IHC, but they also help Histology embed and 
cut paraffin blocks when needed (which is most days).  For 2015 we are 
averaging 4,022 IHC patient test slides and 1,650 IHC control slides per month. 
 Our IHC lab is staffed from 4:30 AM to 6 PM (or later depending on workload).  
We have 5 Leica Bond Max platforms; however, we still do some IHCs on the 
bench.  We usually do 2-3 runs per day and an overnight run (except on Fridays) 
as well.

Richard

Richard W. Cartun, MS, PhD
Director, Histology  Immunopathology
Director, Biospecimen Collection Programs
Assistant Director, Anatomic Pathology
Hartford Hospital
80 Seymour Street
Hartford, CT  06102
(860) 972-1596
(860) 545-2204 Fax

-Original Message-
From: Olszewski, Dawn [mailto:dawn.olszew...@sgmc.org]
Sent: Friday, June 19, 2015 10:53 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] IHC turnaround time

Hi Histonetters,
  6-19-15

We are wondering what the average turnaround times for IHC are for other labs.  
We use the Biocare Intellipath instrumentation. It holds up to 50 slides ( 10 
slides per 5 racks) with continuous feed per open rack.  All IHC orders placed 
by 12pm are usually out the same day by 5pm. We average 434 IHC slides per 
month and have a staff of 3 FTE's.

A pathologist has voiced concerns over our IHC output.  We are trying to 
determine best practices for IHC turnaround time as measured from time order 
placed to time of slide delivery.

If you could respond with your IHC TAT including number of techs and average of 
IHC slides monthly, we would appreciate any input you may be able to provide.

Thank you in advance,

Dawn Olszewski, HTL(ASCP)QIHC

___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

This e-mail message, including any attachments, is for the sole use of the 
intended recipient(s) and may contain confidential and privileged information. 
Any unauthorized review, use, disclosure, or distribution is prohibited. If you 
are not the intended recipient, or an employee or agent responsible for 
delivering the message to the intended recipient, please contact the sender by 
reply e-mail and destroy all copies of the original message, including any 
attachments.

___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] FW: IHC turnaround time

[Cartun, Richard]

I apologize; I meant to preface my remarks with, We have separate Histology 
and IHC Labs.  My data may prove more useful to IHC only labs.

Richard

-Original Message-
From: Cartun, Richard [mailto:richard.car...@hhchealth.org] 
Sent: Friday, June 19, 2015 11:21 AM
To: Olszewski, Dawn; histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] IHC turnaround time

I direct a busy and very efficient (I think) IHC lab staffed with 2.875 FTEs 
who spend most of their time doing IHC, but they also help Histology embed and 
cut paraffin blocks when needed (which is most days).  For 2015 we are 
averaging 4,022 IHC patient test slides and 1,650 IHC control slides per month. 
 Our IHC lab is staffed from 4:30 AM to 6 PM (or later depending on workload).  
We have 5 Leica Bond Max platforms; however, we still do some IHCs on the 
bench.  We usually do 2-3 runs per day and an overnight run (except on Fridays) 
as well.

Richard

Richard W. Cartun, MS, PhD
Director, Histology  Immunopathology
Director, Biospecimen Collection Programs Assistant Director, Anatomic 
Pathology Hartford Hospital
80 Seymour Street
Hartford, CT  06102
(860) 972-1596
(860) 545-2204 Fax

-Original Message-
From: Olszewski, Dawn [mailto:dawn.olszew...@sgmc.org]
Sent: Friday, June 19, 2015 10:53 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] IHC turnaround time

Hi Histonetters,
  6-19-15

We are wondering what the average turnaround times for IHC are for other labs.  
We use the Biocare Intellipath instrumentation. It holds up to 50 slides ( 10 
slides per 5 racks) with continuous feed per open rack.  All IHC orders placed 
by 12pm are usually out the same day by 5pm. We average 434 IHC slides per 
month and have a staff of 3 FTE's.

A pathologist has voiced concerns over our IHC output.  We are trying to 
determine best practices for IHC turnaround time as measured from time order 
placed to time of slide delivery.

If you could respond with your IHC TAT including number of techs and average of 
IHC slides monthly, we would appreciate any input you may be able to provide.

Thank you in advance,

Dawn Olszewski, HTL(ASCP)QIHC

___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

This e-mail message, including any attachments, is for the sole use of the 
intended recipient(s) and may contain confidential and privileged information. 
Any unauthorized review, use, disclosure, or distribution is prohibited. If you 
are not the intended recipient, or an employee or agent responsible for 
delivering the message to the intended recipient, please contact the sender by 
reply e-mail and destroy all copies of the original message, including any 
attachments.

___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] Specimen Accessioning .....

[Cartun, Richard]

We have always accessioned specimens with multiple parts as 1, 2, 3, etc. and 
the corresponding paraffin blocks as A, B, C, etc.  We are in the process of 
changing this to letters for the different specimen parts and numbers for the 
blocks.  For those of you who do it this way, have you encountered any 
problems?  For example, if the specimen arrives and the different parts are 
numbered, do you simply convert them to letters?  Thank you.

Richard

Richard W. Cartun, MS, PhD
Director, Histology  Immunopathology
Director, Biospecimen Collection Programs
Assistant Director, Anatomic Pathology
Hartford Hospital
80 Seymour Street
Hartford, CT  06102
(860) 972-1596
(860) 545-2204 Fax


This e-mail message, including any attachments, is for the sole use of the 
intended recipient(s) and may contain confidential and privileged information. 
Any unauthorized review, use, disclosure, or distribution is prohibited. If you 
are not the intended recipient, or an employee or agent responsible for 
delivering the message to the intended recipient, please contact the sender by 
reply e-mail and destroy all copies of the original message, including any 
attachments.
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] RSV+ tissue

[Cartun, Richard]

We recently performed an autopsy on a patient who died of complications related 
to Respiratory Syncytial Virus (RSV).  We were able to label RSV in the 
formalin-fixed lung tissue using immunohistochemistry (or what I now call 
Morphologic Proteomics).  I can provide unstained slides (maybe even a 
paraffin block) for those of you who would find this type of specimen useful.  
If interested, please tell me why you are interested, and provide your name, 
complete mailing address, telephone number, and a FedEx account number for 
shipping.

Richard

Richard W. Cartun, MS, PhD
Director, Histology  Immunopathology
Director, Biospecimen Collection Programs
Assistant Director, Anatomic Pathology
Hartford Hospital
80 Seymour Street
Hartford, CT  06102
(860) 972-1596
(860) 545-2204 Fax


This e-mail message, including any attachments, is for the sole use of the 
intended recipient(s) and may contain confidential and privileged information. 
Any unauthorized review, use, disclosure, or distribution is prohibited. If you 
are not the intended recipient, or an employee or agent responsible for 
delivering the message to the intended recipient, please contact the sender by 
reply e-mail and destroy all copies of the original message, including any 
attachments.
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

Re: [Histonet] Friday Trivia Question: Most specimen on a single case

[Cartun, Richard]

The answer to this question will depend on what type of environment you work 
in.  I work at a large tertiary care hospital with a major emphasis on cancer 
surgery and, as a result, we frequently get major cancer resections (with 
multiple parts) that generate well over 100 paraffin blocks per case.  Also, 
please note that many current protocols for these types of specimen now dictate 
for an increased number of paraffin blocks to be submitted.

Richard
Richard W. Cartun, MS, PhD
Director, Histology  Immunopathology
Director, Biospecimen Collection Programs
Assistant Director, Anatomic Pathology
Hartford Hospital
80 Seymour Street
Hartford, CT  06102
(860) 972-1596
(860) 545-2204 Fax

-Original Message-
From: Michael Mihalik [mailto:m...@pathview.com]
Sent: Friday, May 08, 2015 12:41 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Friday Trivia Question: Most specimen on a single case

Please excuse the trivia query, but we've got a client who somewhat regularly 
creates cases with 100+ specimen.  I think the most I have ever seen is 127.

I'm curious how common this is.  What's the most specimen on a single case 
you've ever seen?

Thanks for your patience and experience.

Michael Mihalik
PathView Systems | cell: 214.733.7688 | 800.798.3540 | fax: 952.241.7369



___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

This e-mail message, including any attachments, is for the sole use of the 
intended recipient(s) and may contain confidential and privileged information. 
Any unauthorized review, use, disclosure, or distribution is prohibited. If you 
are not the intended recipient, or an employee or agent responsible for 
delivering the message to the intended recipient, please contact the sender by 
reply e-mail and destroy all copies of the original message, including any 
attachments.

___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] Chlamydia control tissue

[Cartun, Richard]

I have asked this in the past without any success, but I need to ask again, 
Does anyone have a control tissue for Chlamydia that they would be willing to 
share?  Thank you.

Richard

Richard W. Cartun, MS, PhD
Director, Histology  Immunopathology
Director, Biospecimen Collection Programs
Assistant Director, Anatomic Pathology
Hartford Hospital
80 Seymour Street
Hartford, CT  06102
(860) 972-1596
(860) 545-2204 Fax


This e-mail message, including any attachments, is for the sole use of the 
intended recipient(s) and may contain confidential and privileged information. 
Any unauthorized review, use, disclosure, or distribution is prohibited. If you 
are not the intended recipient, or an employee or agent responsible for 
delivering the message to the intended recipient, please contact the sender by 
reply e-mail and destroy all copies of the original message, including any 
attachments.
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] Pathology software

[Cartun, Richard]

Can anyone recommend an inexpensive pathology software package for a small 
Dermatopathology laboratory?

Richard

Richard W. Cartun, MS, PhD
Director, Histology  Immunopathology
Director, Biospecimen Collection Programs
Assistant Director, Anatomic Pathology
Hartford Hospital
80 Seymour Street
Hartford, CT  06102
(860) 972-1596
(860) 545-2204 Fax


This e-mail message, including any attachments, is for the sole use of the 
intended recipient(s) and may contain confidential and privileged information. 
Any unauthorized review, use, disclosure, or distribution is prohibited. If you 
are not the intended recipient, or an employee or agent responsible for 
delivering the message to the intended recipient, please contact the sender by 
reply e-mail and destroy all copies of the original message, including any 
attachments.
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet