Re: [Histonet] IHC, decalcification preferences
I, personally, think EDTA SUCKS. a) it takes too long b) its not safe - http://en.wikipedia.org/wiki/EDTA#Toxicity_and_environmental_considerations c) its to old-fashioned. Me, I'm a big proponent of Formic Acid decal. Holla off-list if you want me to hit you up with a protocol, yo. From: Amy Porter port...@msu.edu To: histonet@lists.utsouthwestern.edu; Nicole Collette collet...@mail.llnl.gov Sent: Wednesday, April 1, 2009 12:56:43 PM Subject: Re: [Histonet] IHC, decalcification preferences All the mouse femurs and tibias done in our laboratory are decaled in 14% EDTA and we have a high success rate with our immunohistochemistry and Tunel staining. Amy S. Porter, HT (ASCP) QIHC Investigative HistoPathology Laboratory - Supervisor 2201 Biomedical Physical Sciences Bldg. Rm #2133 East Lansing, MI 48824-3320 Phone: (517) 884-5026 Fax: (517) 432-1368 Email: port...@msu.edu Web: www.humanpathology.msu.edu - Original Message - From: Nicole Collette collet...@mail.llnl.gov To: histonet@lists.utsouthwestern.edu Sent: Wednesday, April 01, 2009 12:44 PM Subject: [Histonet] IHC, decalcification preferences Hello, all, I am going to be doing some IHC on adult mouse bone, and since I'm new to all this antigen preservation stuff, was wondering the best way to decalcify? Normally, I use high percentage EDTA (17%) and determine end point by weight loss/weight gain. (up until now have been using this protocol for LacZ stains, works like a charm! ) We are comparing several lines of mice with bone mineral density differences, and endpoint is very phenotype and age-dependent. It takes a long time, but the results are worth the wait. I also have Cal-Rite, which is a formaldehyde/formic acid decalcifier, and obviously works much faster. I am leaning toward the EDTA, since it seems to work well for the LacZ, which is sensitive to lots of other processes, but if there's a reason not to, please let me know. Thanks for all your help and support, I have so far been given great advice from this listserv. Sincerely, Nicole Collette LLNL/UCB ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] So... I Need Some Expert Advice:
I suggest to start a board/forum. Really. On Wed, 1 Apr 2009 23:13:09 -0700 (PDT), aa aa lactose.intoler...@yahoo.com wrote: Dear Histotech Experts! I am working on a very complicated project, and could really use some advice from both the guru-level histologists and also from the go-getter know-how of the histotechs Because it's sort of hard to explain (and involves some comparatively obscure staining techniques and mounting methods) I've put together a short slide-show to help cut-to-the-chase, as it were, because frankly, some things are just too tedious, abstract and confusing to type out in text. Anyway, on with the show! I would truly and thankfully appreciate any and all commentary and advice! Please view my detailed presentation here: http://tinyurl.com/2g9mqh Thanks Everybody! ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] So... I Need Some Expert Advice:
What I got was a Rick Roll music video on Youtube. If aa aa is a spammer, this was a slick way to attract our attention. Anybody else? aa aa wrote: Dear Histotech Experts! I am working on a very complicated project, and could really use some advice from both the guru-level histologists and also from the go-getter know-how of the histotechs Because it's sort of hard to explain (and involves some comparatively obscure staining techniques and mounting methods) I've put together a short slide-show to help cut-to-the-chase, as it were, because frankly, some things are just too tedious, abstract and confusing to type out in text. Anyway, on with the show! I would truly and thankfully appreciate any and all commentary and advice! Please view my detailed presentation here: http://tinyurl.com/2g9mqh Thanks Everybody! ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
FW: [Histonet] So... I Need Some Expert Advice:
Mark, Histology, who cares, turned up the volume and danced round the office singing along to this Stock, Aitken and Waterman classic. This soul boy even has the album on his iPhone. Ian. Dr. Ian Montgomery, Histotechnology, I.B.L.S. Support Unit, Thomson Building, University of Glasgow, Glasgow, G12 8QQ. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Mark Ray Sent: 02 April 2009 10:10 To: lactose.intoler...@yahoo.com Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] So... I Need Some Expert Advice: What I got was a Rick Roll music video on Youtube. If aa aa is a spammer, this was a slick way to attract our attention. Anybody else? aa aa wrote: Dear Histotech Experts! I am working on a very complicated project, and could really use some advice from both the guru-level histologists and also from the go-getter know-how of the histotechs Because it's sort of hard to explain (and involves some comparatively obscure staining techniques and mounting methods) I've put together a short slide-show to help cut-to-the-chase, as it were, because frankly, some things are just too tedious, abstract and confusing to type out in text. Anyway, on with the show! I would truly and thankfully appreciate any and all commentary and advice! Please view my detailed presentation here: http://tinyurl.com/2g9mqh Thanks Everybody! ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] So... I Need Some Expert Advice:
I think it was April fool... -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Mark Ray Sent: Thursday, April 02, 2009 5:10 AM To: lactose.intoler...@yahoo.com Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] So... I Need Some Expert Advice: What I got was a Rick Roll music video on Youtube. If aa aa is a spammer, this was a slick way to attract our attention. Anybody else? aa aa wrote: Dear Histotech Experts! I am working on a very complicated project, and could really use some advice from both the guru-level histologists and also from the go-getter know-how of the histotechs Because it's sort of hard to explain (and involves some comparatively obscure staining techniques and mounting methods) I've put together a short slide-show to help cut-to-the-chase, as it were, because frankly, some things are just too tedious, abstract and confusing to type out in text. Anyway, on with the show! I would truly and thankfully appreciate any and all commentary and advice! Please view my detailed presentation here: http://tinyurl.com/2g9mqh Thanks Everybody! ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This email, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please reply to the sender that you have received the message in error, then delete this message. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Off topic posts
KIDS, KIDS!!! Enough is enough. Take it off the list and respond to one another privately. It's fast becoming like observing a daycare here. Glen Dawson IHC Manager Milwaukee, WI ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Off topic posts
Take it off the list and respond to one another privately. Says he on the list and not privately From: Dawson, Glen gdaw...@dynacaremilwaukee.com To: Histonet histonet@lists.utsouthwestern.edu Sent: Thursday, April 2, 2009 8:52:28 AM Subject: RE: [Histonet] Off topic posts KIDS, KIDS!!! Enough is enough. Take it off the list and respond to one another privately. It's fast becoming like observing a daycare here. Glen Dawson IHC Manager Milwaukee, WI ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] The end of Histonet
It is getting difficult to remember when an item that actually dealt with a histotechnology issue was posted. This is how worthwhile discussion groups fail, endless prattle followed by endless prattle about the endless prattle. Geoff -- ** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583 mcaul...@umdnj.edu ** ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] So... I Need Some Expert Advice:
Looked like a scam to me so I hit Delete immediately. No real name, no real address = scam Geoff Mark Ray wrote: What I got was a Rick Roll music video on Youtube. If aa aa is a spammer, this was a slick way to attract our attention. Anybody else? aa aa wrote: Dear Histotech Experts! I am working on a very complicated project, and could really use some advice from both the guru-level histologists and also from the go-getter know-how of the histotechs Because it's sort of hard to explain (and involves some comparatively obscure staining techniques and mounting methods) I've put together a short slide-show to help cut-to-the-chase, as it were, because frankly, some things are just too tedious, abstract and confusing to type out in text. Anyway, on with the show! I would truly and thankfully appreciate any and all commentary and advice! Please view my detailed presentation here: http://tinyurl.com/2g9mqh Thanks Everybody! ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- -- ** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583 mcaul...@umdnj.edu ** ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Fc receptor blocker for staph
Does anyone know where you can purchase an Fc receptor blocker for staph? Vendors welcome! Thanks! Jeanine Bartlett, BS, HT(ASCP)QIHC Centers for Disease Control and Prevention Infectious Diseases Pathology Branch 1600 Clifton Road, MS/G-32 18/SB-114 Atlanta, GA 30333 (404) 639-3590 jeanine.bartl...@cdc.hhs.gov ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Re: The end of Histonet
It is getting difficult to remember when an item that actually dealt with a histotechnology issue was posted. This is how worthwhile discussion groups fail, endless prattle followed by endless prattle about the endless prattle. And you plan to remedy this by posting more prattle about the prattle about the endless prattle? From: Geoff McAuliffe mcaul...@umdnj.edu To: Bernie Taupin bernietau...@ymail.com Cc: HistoNet histonet@lists.utsouthwestern.edu Sent: Thursday, April 2, 2009 10:10:12 AM Subject: The end of Histonet It is getting difficult to remember when an item that actually dealt with a histotechnology issue was posted. This is how worthwhile discussion groups fail, endless prattle followed by endless prattle about the endless prattle. Geoff -- ** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583 mcaul...@umdnj.edu ** ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] The end of Histonet
Specially when one contributor tries to outsmart or outwit another or have the final saying. René J. --- On Thu, 4/2/09, Geoff McAuliffe mcaul...@umdnj.edu wrote: From: Geoff McAuliffe mcaul...@umdnj.edu Subject: [Histonet] The end of Histonet To: Bernie Taupin bernietau...@ymail.com Cc: HistoNet histonet@lists.utsouthwestern.edu Date: Thursday, April 2, 2009, 10:10 AM It is getting difficult to remember when an item that actually dealt with a histotechnology issue was posted. This is how worthwhile discussion groups fail, endless prattle followed by endless prattle about the endless prattle. Geoff -- ** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583 mcaul...@umdnj.edu ** ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] So... I Need Some Expert Advice:
Looked like a scam to me so I hit Delete immediately. No real name, no real address = scam It was a link to a video, I believe, not a scam. From: Geoff McAuliffe mcaul...@umdnj.edu To: Mark Ray darkd...@comcast.net Cc: histonet@lists.utsouthwestern.edu Sent: Thursday, April 2, 2009 10:12:30 AM Subject: Re: [Histonet] So... I Need Some Expert Advice: Looked like a scam to me so I hit Delete immediately. No real name, no real address = scam Geoff Mark Ray wrote: What I got was a Rick Roll music video on Youtube. If aa aa is a spammer, this was a slick way to attract our attention. Anybody else? aa aa wrote: Dear Histotech Experts! I am working on a very complicated project, and could really use some advice from both the guru-level histologists and also from the go-getter know-how of the histotechs Because it's sort of hard to explain (and involves some comparatively obscure staining techniques and mounting methods) I've put together a short slide-show to help cut-to-the-chase, as it were, because frankly, some things are just too tedious, abstract and confusing to type out in text. Anyway, on with the show! I would truly and thankfully appreciate any and all commentary and advice! Please view my detailed presentation here: http://tinyurl.com/2g9mqh Thanks Everybody! ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- -- ** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583 mcaul...@umdnj.edu ** ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] So... I Need Some Expert Advice:
It wasn't funky it was a good video - but it had no relevance to what we are supposed to be doing here on histonet. Now if somebody could post the link to some Adam Lambert tunes... Andi;-) On Apr 2, 2009, at 2:10 AM, Mark Ray wrote: What I got was a Rick Roll music video on Youtube. If aa aa is a spammer, this was a slick way to attract our attention. Anybody else? aa aa wrote: Dear Histotech Experts! I am working on a very complicated project, and could really use some advice from both the guru-level histologists and also from the go-getter know-how of the histotechs Because it's sort of hard to explain (and involves some comparatively obscure staining techniques and mounting methods) I've put together a short slide-show to help cut-to-the-chase, as it were, because frankly, some things are just too tedious, abstract and confusing to type out in text. Anyway, on with the show! I would truly and thankfully appreciate any and all commentary and advice! Please view my detailed presentation here: http://tinyurl.com/2g9mqh Thanks Everybody! ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Andrea Grantham, HT (ASCP) Senior Research Specialist University of Arizona Cell Biology and Anatomy Histology Service Laboratory P.O.Box 245044 Tucson, AZ 85724 algra...@email.arizona.edu Tel: 520.626.4415 Fax: 520.626.2097 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] glutaraldehyde perfusion
Can someone explain to me the mechanism by which glutaraldehyde would disrupt the blood brain barrier if the brain had not been flushed prior to fixation? Boma. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] skin sectioning
Greetings, I have been cutting sections of whole bat genitalia embedded in JB-4. My intention is to study and photograph these sections in light microscopy. The quality of the sections is excellent except for one detail. The medium separates from the epidermis (but not any other tissue interface). At first I thought it was the hair, but the separation occurred even if I trimmed the hairs. I also tried to section skin from the bat's torso and had similar results. I have left the specimens to impregnate literally for days. The bat's penis is about 4mm long and 1mm in diameter at the base; female parts are slightly wider and shorter. My lab is set up for sectioning with glass knives and I wanted to avoid the shrinkage of paraffin. Can anyone suggest a method to avoid this problem with the JB-4, or another medium? Winston C. Lancaster Dept. of Biological Sciences California State University, Sacramento Sacramento, California 95819 6077 wlancas...@csus.edu ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
histonet@lists.utsouthwestern.edu
Trumps Fixative Ten ml of 40% formaldehyde and 2 ml of 50% glutaraldehyde were added to 200-mOsm buffer. This solution could be conveniently prepared for routine use as follows: 1.16 g of NaH[2]PO[4] is added to 88 ml water and dissolved. Add 0.27 g NaOH followed by the formaldehyde and the glutaraldehyde. Ref. McDowell, E and B. Trump. 1976. Histological fixatives for diagnostic light and electron microscopy. Arch. Pathol. Lab. Med. 100:405-414. Michael A Safron A.S., HT (ASCP)CM WIL Research Laboratories LLC Manager, Histology 419-289-8700 ext 3040 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] skin JB-4
Greetings, I have a project sectioning whole bat genital organs. The study, in part, involves measurement and so to avoid shrinkage I am using JB-4 and cutting with a glass knife. The tissue comes out very well except for one problem. The epidermis pulls away from the medium. I have tried leaving the tissue to infiltrate in the catalyzed resin for days and trimming all the little hairs and bristles), to no avail (have not tried bikini waxing as I don't want to destroy the follicles. Later I embedded a patch of skin from the torso and had the same results. Has anyone seen this before? Were you able to solve it? Is there another medium that anyone could suggest that will minimize shrinkage while also adhering to the epidermis? Thanks, Winston Winston C. Lancaster Dept. of Biological Sciences California State University, Sacramento Sacramento, California 95819 6077 wlancas...@csus.edu ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Tissue Processor
FREE TBS Tissue Processor (you pay shipping). Kind of a lemon. Liesl Frasier 719-530-2200 x2195 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] GemCut Colored Paraffin
Do any of you know if this colored paraffin by Polysciences, Inc. stains the tissue and if so would it auto fluoresce? Andrea Grantham, HT (ASCP) Senior Research Specialist University of Arizona Cell Biology and Anatomy Histology Service Laboratory P.O.Box 245044 Tucson, AZ 85724 algra...@email.arizona.edu Tel: 520.626.4415 Fax: 520.626.2097 happy slicing and dicing and may all your stains work perfectly - Paula Sicurello ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] glutaraldehyde perfusion
Hi Boma: Whether or not a saline or buffer flush was used before glut. fixation is of little consequence to the effects of glut as a fixative.The saline/buffer washout is just to remove blood. In my experience most people overdo the washout. I am curious as to what disrupt the blood-brain barrier means. The bbb is a very complex entity with both physical and metabolic components.I suspect the person who told you this is not well informed. Geoff Boma Fubara wrote: Can someone explain to me the mechanism by which glutaraldehyde would disrupt the blood brain barrier if the brain had not been flushed prior to fixation? Boma. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- -- ** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583 mcaul...@umdnj.edu ** ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Let's call a truce
Histonet members: As the list moderator, I now feel the need to ask everyone to please get back to the business of histology and related topics. Histonet is run using the University of Texas Southwestern Medical Center's resources and I suspect they would not look favorably on large volumes of off-topic messages. As everyone knows, Histonet is a very useful resource because of the tremendous contributions of all the members (now up to 3200 people) and it would be a shame if a University official happened to review the recent discourse and decide this wasn't a good use of Texas tax dollars. Thanks so much, Linda M Histonet administrator Bernie Taupin bernietau...@ymail.com 4/1/2009 11:04 PM At this point, I think about 20 or more different people have been involved in this thread! Would anyone else like to chime in with their two-cents about being on-topic or offer their inane peanut-gallery observations and silly tit-for-tat bickering like Paula here? From: Va Paula Sicurello vapat...@yahoo.com To: HistoNet histonet@lists.utsouthwestern.edu Sent: Wednesday, April 1, 2009 1:10:30 PM Subject: [Histonet] Let's call a truce Hello Netters, Time to stop the current off topic conversations and get back to histology. I apologize to Bernie for my comment since I contributed to this off topic topic. It's a new month let's start fresh. Happy slicing and dicing and may all your stains work perfectly. Paula :-) Paula Sicurello VA Medical Center San Diego Veterans Medical Research Foundation (VMRF) Core Research Imaging Center 3350 La Jolla Village Dr., MC151 San Diego, CA 92161 858-552-8585 x2397 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet /pre span style=font-weight: bold;Please consider the environment before printing this e-mail/spanbr / br / span style=font-size: 8pt;This e-mail, facsimile, or letter and any files or attachments transmitted with it containsbr / information that is confidential and privileged. This information is intended only for the use of the br / individual(s) and entity(ies) to whom it is addressed. If you are the intended recipient, further br / disclosures are prohibited without proper authorization. If you are not the intended recipient, any br / disclosure, copying, printing, or use of this information is strictly prohibited and possibly a br / violation of federal or state law and regulations. If you have received this information in error, br / please notify Children's Medical Center Dallas immediately at 214-456- or via e-mail at br / priv...@childrens.com. Children's Medical Center Dallas and its affiliates hereby claim all br / applicable privileges related to this information./spanbr / br / /html ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] So... I Need Some Expert Advice:
Come on people. Yesterday was April Fool's Day and Histonet got Rick Rolled. Today is the 2nd, move on. PKP From: Andrea Grantham algra...@email.arizona.edu To: HISTONET histonet@lists.utsouthwestern.edu Sent: Thursday, April 2, 2009 9:33:06 AM Subject: Re: [Histonet] So... I Need Some Expert Advice: It wasn't funky it was a good video - but it had no relevance to what we are supposed to be doing here on histonet. Now if somebody could post the link to some Adam Lambert tunes... Andi;-) On Apr 2, 2009, at 2:10 AM, Mark Ray wrote: What I got was a Rick Roll music video on Youtube. If aa aa is a spammer, this was a slick way to attract our attention. Anybody else? aa aa wrote: Dear Histotech Experts! I am working on a very complicated project, and could really use some advice from both the guru-level histologists and also from the go-getter know-how of the histotechs Because it's sort of hard to explain (and involves some comparatively obscure staining techniques and mounting methods) I've put together a short slide-show to help cut-to-the-chase, as it were, because frankly, some things are just too tedious, abstract and confusing to type out in text. Anyway, on with the show! I would truly and thankfully appreciate any and all commentary and advice! Please view my detailed presentation here: http://tinyurl.com/2g9mqh Thanks Everybody! ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Andrea Grantham, HT (ASCP) Senior Research Specialist University of Arizona Cell Biology and Anatomy Histology Service Laboratory P.O.Box 245044 Tucson, AZ 85724 algra...@email.arizona.edu Tel: 520.626.4415 Fax: 520.626.2097 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] So... I Need Some Expert Advice:
Might I suggest that what we need on Histonet is to deal with some basic questions in histology that have never been answered in detail but have only been touched upon. Histonet has a great deal of expertise and it seems we should be asking these major questions. Topics such as what is optimal time of fixing, best waxes etc. and why should be issues here. My perception is that we often will give personal opinions as to what the best techniques or materials are but not the science behind the rational for choosing these, maybe because we do not know. However I feel that this is an area that we need to expand. Hope that this makes sense. I've had a week of family medical issues, broken plumbing under the house (guess that better than personal plumbing being broken!), two computer crashes and fire ants to name only the major ones. There is therefore, in my opinion, no way to go but up from here so can we hear your views please? Barry ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Mouse mammary glands
Dear histonetters, I am in need of some help. Is it possible to stain a mouse mammary gland in Carmine Alum after it has been fixed in 4% PFA? We originally were fixing them in Carnoy's and then staining, but then it was decided not to stain anymore and the glands were just fixed with the PFA. Now, it looks like the glands will need to be stained, not only the currently procured ones, but also the previous ones as well. If this can be done, does anyone have a procedure or any suggestions? Thank you, Kim Land University of Southern Indiana 8600 University Blvd. Evansville, IN 47712 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Embedding cell pellets
I am looking for a protocol for embedding cell pellets in paraffin. Thanks, Louise Louise Hartson, BA Senior Technical Associate University of Rochester louise_hart...@urmc.rochester.edu ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] GemCut Colored Paraffin
We use this paraffin and it does not cause any permanent color change to the tissue. When it is removed from the processor after infiltration, the tissue does have some color, but after deparaffinization during staining, there is no color left. Hope this helps. Emily M. Lockman, HT (ASCP) Histotechnologist II, Pathology Services American Preclinical Services, LLC (APS) 8945 Evergreen Boulevard Minneapolis, MN 55433 Phone: 763-717-7990 Fax: 763-717-2042 elock...@apsemail.com -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Andrea Grantham Sent: Thursday, April 02, 2009 10:04 AM To: HISTONET Subject: [Histonet] GemCut Colored Paraffin Do any of you know if this colored paraffin by Polysciences, Inc. stains the tissue and if so would it auto fluoresce? Andrea Grantham, HT (ASCP) Senior Research Specialist University of Arizona Cell Biology and Anatomy Histology Service Laboratory P.O.Box 245044 Tucson, AZ 85724 algra...@email.arizona.edu Tel: 520.626.4415 Fax: 520.626.2097 happy slicing and dicing and may all your stains work perfectly - Paula Sicurello ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Strange circles in IHC slides
Hello, I really have some real links to real pictures of real relevance of the real histonet list. Really promised. http://img12.imageshack.us/img12/8513/ts0402162049.jpg http://img13.imageshack.us/img13/6514/ts0402162104.jpg So, has ever anyone experienced sth. like this? My conjugate control (every step except the antibody) was fine, nothing to be seen about DAB and no circles at all. I used Shandon single-use coverplates, sterile buffer, fresh antibody aliquots. Any idea? Thanks, V. Neubert ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] So... I Need Some Expert Advice:
I'm not in total disagreement with Barry. The FAQ is a good place to start, and it might be all we need, but I wonder it shouldn't be revisited just to be sure. There seems to be a lot of questions lately on some of the basics as well as queries about instrumentation. My question would be this: In light of different internet technologies, would a blog format be an acceptable way of handling or grouping some of these issues? I'm certainly not advocating a change in Histonet, as it is a fine format that is serving the community well. I'm just offering this as a possibile answer to Barry's concern, if others find his concern worth looking into. Just a thought. William (Bill) O'Donnell, HT (ASCP) QIHC Lead Histologist Good Samaritan Hospital 10 East 31st Street Kearney, NE 68847 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Rittman, Barry R Sent: Thursday, April 02, 2009 10:45 AM To: HISTONET Subject: RE: [Histonet] So... I Need Some Expert Advice: Might I suggest that what we need on Histonet is to deal with some basic questions in histology that have never been answered in detail but have only been touched upon. Histonet has a great deal of expertise and it seems we should be asking these major questions. Topics such as what is optimal time of fixing, best waxes etc. and why should be issues here. My perception is that we often will give personal opinions as to what the best techniques or materials are but not the science behind the rational for choosing these, maybe because we do not know. However I feel that this is an area that we need to expand. Hope that this makes sense. I've had a week of family medical issues, broken plumbing under the house (guess that better than personal plumbing being broken!), two computer crashes and fire ants to name only the major ones. There is therefore, in my opinion, no way to go but up from here so can we hear your views please? Barry ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Embedding cell pellets
Histogel works well for this purpose too. --Original Message-- From: Thomas Pier Sender: histonet-boun...@lists.utsouthwestern.edu To: histonet@lists.utsouthwestern.edu To: louise_hart...@urmc.rochester.edu Sent: Apr 2, 2009 12:17 PM Subject: Re: [Histonet] Embedding cell pellets Fix the cells, spin them down, decant, and resuspend in 1% agarose. The agar plug can be processed and embedded like any other piece of tissue. Tom Pier Hartson, Louise louise_hart...@urmc.rochester.edu 04/02/09 11:08 AM I am looking for a protocol for embedding cell pellets in paraffin. Thanks, Louise Louise Hartson, BA Senior Technical Associate University of Rochester louise_hart...@urmc.rochester.edu ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] So... I Need Some Expert Advice (forum for information):
True true that would be cool. But I don't mind repeated topics being discussed again and again - it isn't always a bad thing, as although histology is a very old and largely well-established science, there is room for improvements and modifications over time.so I appreciate the ongoing discussions over the years, even if a lot of things get repeated. And there ARE a lot of protocols and stable forum websites out there for histo stuff. As fab as Histonet is, it is one of many online resources I consult daily. Andrea -Original Message- From: Nicole Collette collet...@mail.llnl.gov Date: Thu, 02 Apr 2009 09:43:04 To: HISTONEThistonet@lists.utsouthwestern.edu Subject: RE: [Histonet] So... I Need Some Expert Advice (forum for information): Hi, All, I'm not generally one to put in my two cents' worth, but today I'm feeling especially chipper. In my world, (idealistic optimist that I am), I would love to see something like a cross between PLoS One, Wikipedia, and Cold Spring Harbor protocols- A mainstream, accepted (or widely used but still not definitive) protocol, or commonly used equipment, with links to discussion for individual steps or reagents. For example, fixing tissue would list a set of reagents, a basic process, and reagents or steps that are often up for debate (Why use NBF? what is paraformaldehyde? what does glutaraldehyde do? How do you get that picric acid yellow out?) would be associated with a link to discussion, so that people who are just looking for the facts can have them readily available, without side comments, and those who want to know the nitty gritty details that cause controversy can make their own judgements, or add their own comments. Now, I'm not at all technologically able to build this resource, but would give anyone who wanted to take it on an Attaboy! or Attagirl! And if you do, please send me the link ;) There's a lot of good info here, and some really great expertise, but finding it threaded through the archives is not the easiest way to find this stuff, and it is evident by the discussions that come up over and over. Thanks for listening! Keep up the good work! Sincerely, Nicole Collette _ At 10:13 AM -0600 4/2/09, O'Donnell, Bill wrote: I'm not in total disagreement with Barry. The FAQ is a good place to start, and it might be all we need, but I wonder it shouldn't be revisited just to be sure. There seems to be a lot of questions lately on some of the basics as well as queries about instrumentation. My question would be this: In light of different internet technologies, would a blog format be an acceptable way of handling or grouping some of these issues? I'm certainly not advocating a change in Histonet, as it is a fine format that is serving the community well. I'm just offering this as a possibile answer to Barry's concern, if others find his concern worth looking into. Just a thought. William (Bill) O'Donnell, HT (ASCP) QIHC Lead Histologist Good Samaritan Hospital 10 East 31st Street Kearney, NE 68847 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Rittman, Barry R Sent: Thursday, April 02, 2009 10:45 AM To: HISTONET Subject: RE: [Histonet] So... I Need Some Expert Advice: Might I suggest that what we need on Histonet is to deal with some basic questions in histology that have never been answered in detail but have only been touched upon. Histonet has a great deal of expertise and it seems we should be asking these major questions. Topics such as what is optimal time of fixing, best waxes etc. and why should be issues here. My perception is that we often will give personal opinions as to what the best techniques or materials are but not the science behind the rational for choosing these, maybe because we do not know. However I feel that this is an area that we need to expand. Hope that this makes sense. I've had a week of family medical issues, broken plumbing under the house (guess that better than personal plumbing being broken!), two computer crashes and fire ants to name only the major ones. There is therefore, in my opinion, no way to go but up from here so can we hear your views please? Barry ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http:// lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http:// lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] low profile blades and cutting angle
Hi everyone, What brand low profile blades does everyone prefer and where do you order them? Also, what blade angle does everyone prefer for cryosectioning? Thanks Stacey ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Let's call a truce
Boy Bernie, See if I ever buy your albums again! ;-) Paula Sicurello VA Medical Center San Diego Veterans Medical Research Foundation (VMRF) Core Research Imaging Center 3350 La Jolla Village Dr., MC151 San Diego, CA 92161 858-552-8585 x2397 --- On Thu, 4/2/09, Bernie Taupin bernietau...@ymail.com wrote: From: Bernie Taupin bernietau...@ymail.com Subject: Re: [Histonet] Let's call a truce To: HistoNet histonet@lists.utsouthwestern.edu Date: Thursday, April 2, 2009, 4:04 AM At this point, I think about 20 or more different people have been involved in this thread! Would anyone else like to chime in with their two-cents about being on-topic or offer their inane peanut-gallery observations and silly tit-for-tat bickering like Paula here? From: Va Paula Sicurello vapat...@yahoo.com To: HistoNet histo...@lists..utsouthwestern.edu Sent: Wednesday, April 1, 2009 1:10:30 PM Subject: [Histonet] Let's call a truce Hello Netters, Time to stop the current off topic conversations and get back to histology. I apologize to Bernie for my comment since I contributed to this off topic topic. It's a new month let's start fresh. Happy slicing and dicing and may all your stains work perfectly. Paula :-) Paula Sicurello VA Medical Center San Diego Veterans Medical Research Foundation (VMRF) Core Research Imaging Center 3350 La Jolla Village Dr., MC151 San Diego, CA 92161 858-552-8585 x2397 ___ Histonet mailing list histo...@lists..utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list histo...@lists.utsouthwestern..edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Dummy question on the old Fisher Histomatic tissue processor 166A
Hi all, What exactly is it supposed to do when the switch Process mode (start program-drain-purge) switch is set to purge? I aquired this one allmost for free and I will use it - once it's up and running - in the first place for plant anatomy. Yep: it's some kind of a museum around here. Yvan. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] low profile blades and cutting angle
AccuEdge by Sakura Finetek are the best (avail. through VWR) Not only have I tried a few other brands, but our former knowledgeable and experienced histotech here at UB recommends AccuEdge. Merced --On Thursday, April 02, 2009 9:55 AM -0700 Stacey Barrick barricksta...@yahoo.com wrote: Hi everyone, What brand low profile blades does everyone prefer and where do you order them? Also, what blade angle does everyone prefer for cryosectioning? Thanks Stacey ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Merced M Leiker Research Technician II 354 Biomedical Research Building School of Medicine and Biomedical Sciences State University of New York at Buffalo 3435 Main St, Buffalo, NY 14214 Ph: (716) 829-6033 Fx: (716) 829-2725 No trees were harmed in the sending of this email. However, many electrons were severely inconvenienced. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Embedding cell pellets
Try histogel from Richard Allan- available from Fisher.Easier than making up agarose! Bernice Bernice Frederick HTL (ASCP) Northwestern University Pathology Core Facility ECOGPCO-RL 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Thomas Pier Sent: Thursday, April 02, 2009 11:18 AM To: histonet@lists.utsouthwestern.edu; louise_hart...@urmc.rochester.edu Subject: Re: [Histonet] Embedding cell pellets Fix the cells, spin them down, decant, and resuspend in 1% agarose. The agar plug can be processed and embedded like any other piece of tissue. Tom Pier Hartson, Louise louise_hart...@urmc.rochester.edu 04/02/09 11:08 AM I am looking for a protocol for embedding cell pellets in paraffin. Thanks, Louise Louise Hartson, BA Senior Technical Associate University of Rochester louise_hart...@urmc.rochester.edu ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] low profile blades and cutting angle
It's all I ever use and I've tried a bunch. Bernice Bernice Frederick HTL (ASCP) Northwestern University Pathology Core Facility ECOGPCO-RL 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Merced Leiker Sent: Thursday, April 02, 2009 12:17 PM To: barricksta...@yahoo.com; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] low profile blades and cutting angle AccuEdge by Sakura Finetek are the best (avail. through VWR) Not only have I tried a few other brands, but our former knowledgeable and experienced histotech here at UB recommends AccuEdge. Merced --On Thursday, April 02, 2009 9:55 AM -0700 Stacey Barrick barricksta...@yahoo.com wrote: Hi everyone, What brand low profile blades does everyone prefer and where do you order them? Also, what blade angle does everyone prefer for cryosectioning? Thanks Stacey ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Merced M Leiker Research Technician II 354 Biomedical Research Building School of Medicine and Biomedical Sciences State University of New York at Buffalo 3435 Main St, Buffalo, NY 14214 Ph: (716) 829-6033 Fx: (716) 829-2725 No trees were harmed in the sending of this email. However, many electrons were severely inconvenienced. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] GemCut Parafin
Does anyone use GemCut parafin, and what do you think about it, any good, any concerns? Steve ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Burned Tissue
Recently we are experiencing random blocks of tissue (approx. 3 blocks out of 400/2x) that appear to be burned.? The tissue is prostate and the staining looks dark.? Upon recutting, sometimes it is better, sometimes it is not, which negates my ability to rule out the stainer completely. We process on a 2 hour schedule using the Peloris and all tissue is processed together.? I am not understanding how, out of a 12 part prostate, only one block stains so dark so randomly.? When recut, some blocks are better, some still have the dark staining. Does anyone have any suggestions? Ann ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] low profile blades and cutting angle
We use MX premier microtome blades and order from ThermoFisher. I do have the accuedge blades but prefer the MX blades. The accuedge blades just don't seem to be as good as they used to be...my opinion only. Hazel Horn Hazel Horn, HT/HTL (ASCP) Supervisor of Histology Arkansas Children's Hospital 1 Children's WaySlot 820 Little Rock, AR 72202 phone 501.364.4240 fax501.364.3155 visit us on the web at:www.archildrens.org -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Bernice Frederick Sent: Thursday, April 02, 2009 1:20 PM To: 'Merced Leiker'; barricksta...@yahoo.com; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] low profile blades and cutting angle It's all I ever use and I've tried a bunch. Bernice Bernice Frederick HTL (ASCP) Northwestern University Pathology Core Facility ECOGPCO-RL 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Merced Leiker Sent: Thursday, April 02, 2009 12:17 PM To: barricksta...@yahoo.com; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] low profile blades and cutting angle AccuEdge by Sakura Finetek are the best (avail. through VWR) Not only have I tried a few other brands, but our former knowledgeable and experienced histotech here at UB recommends AccuEdge. Merced --On Thursday, April 02, 2009 9:55 AM -0700 Stacey Barrick barricksta...@yahoo.com wrote: Hi everyone, What brand low profile blades does everyone prefer and where do you order them? Also, what blade angle does everyone prefer for cryosectioning? Thanks Stacey ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Merced M Leiker Research Technician II 354 Biomedical Research Building School of Medicine and Biomedical Sciences State University of New York at Buffalo 3435 Main St, Buffalo, NY 14214 Ph: (716) 829-6033 Fx: (716) 829-2725 No trees were harmed in the sending of this email. However, many electrons were severely inconvenienced. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ** The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] HT licensing in 2011
Does anyone know about a law that is supposed to go into effect in 2011 that will require histotechs to be licensed? I also heard something about a person being eligible to take the HT exam if they get a state license - which can be obtained by simply paying a fee if they have 5 years of work experience? Laurie Colbert ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] low profile blades and cutting angle
I am with you Hazel. We cut paraffin and today I am cutting GMA plastic with them. They are great for us. Pam Marcum UPENN Vet School New Bolton Center - Original Message - From: Hazel V Horn hor...@archildrens.org To: histonet@lists.utsouthwestern.edu Sent: Thursday, April 2, 2009 3:22:09 PM GMT -05:00 US/Canada Eastern Subject: RE: [Histonet] low profile blades and cutting angle We use MX premier microtome blades and order from ThermoFisher. I do have the accuedge blades but prefer the MX blades. The accuedge blades just don't seem to be as good as they used to be...my opinion only. Hazel Horn Hazel Horn, HT/HTL (ASCP) Supervisor of Histology Arkansas Children's Hospital 1 Children's Way Slot 820 Little Rock, AR 72202 phone 501.364.4240 fax 501.364.3155 visit us on the web at: www.archildrens.org -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Bernice Frederick Sent: Thursday, April 02, 2009 1:20 PM To: 'Merced Leiker'; barricksta...@yahoo.com; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] low profile blades and cutting angle It's all I ever use and I've tried a bunch. Bernice Bernice Frederick HTL (ASCP) Northwestern University Pathology Core Facility ECOGPCO-RL 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Merced Leiker Sent: Thursday, April 02, 2009 12:17 PM To: barricksta...@yahoo.com; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] low profile blades and cutting angle AccuEdge by Sakura Finetek are the best (avail. through VWR) Not only have I tried a few other brands, but our former knowledgeable and experienced histotech here at UB recommends AccuEdge. Merced --On Thursday, April 02, 2009 9:55 AM -0700 Stacey Barrick barricksta...@yahoo.com wrote: Hi everyone, What brand low profile blades does everyone prefer and where do you order them? Also, what blade angle does everyone prefer for cryosectioning? Thanks Stacey ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Merced M Leiker Research Technician II 354 Biomedical Research Building School of Medicine and Biomedical Sciences State University of New York at Buffalo 3435 Main St, Buffalo, NY 14214 Ph: (716) 829-6033 Fx: (716) 829-2725 No trees were harmed in the sending of this email. However, many electrons were severely inconvenienced. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ** The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] low profile blades and cutting angle
Stacey, Same for me too with Accuedge! But I also like Shandon MX35 Premier. Pack of 50 blades may be over $100 (depends on your Fisher pricing). I use these for both microtome and cyrostat sections. I've also used these for MMA sections. Premier Plus should be good also, but I haven't tried it. 30-518-35 Thermo Scientific No.:3051835 Blade angle? About 30 degrees. Thermo states 34 degrees in their catalog. I think I've seen Accuedge blades thru several vendor sites. Cardinal, Fisher, VWR, Electron Microscopy Sciences, ect. For example: http://www.emsdiasum.com/microscopy/products/histology/sectioning.aspx Hugh KP-Hawaii From: b-freder...@northwestern.edu To: lei...@buffalo.edu; barricksta...@yahoo.com; histonet@lists.utsouthwestern.edu Date: Thu, 2 Apr 2009 13:19:48 -0500 Subject: RE: [Histonet] low profile blades and cutting angle CC: It's all I ever use and I've tried a bunch. Bernice Bernice Frederick HTL (ASCP) Northwestern University Pathology Core Facility ECOGPCO-RL 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Merced Leiker Sent: Thursday, April 02, 2009 12:17 PM To: barricksta...@yahoo.com; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] low profile blades and cutting angle AccuEdge by Sakura Finetek are the best (avail. through VWR) Not only have I tried a few other brands, but our former knowledgeable and experienced histotech here at UB recommends AccuEdge. Merced --On Thursday, April 02, 2009 9:55 AM -0700 Stacey Barrick barricksta...@yahoo.com wrote: Hi everyone, What brand low profile blades does everyone prefer and where do you order them? Also, what blade angle does everyone prefer for cryosectioning? Thanks Stacey ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _ Rediscover Hotmail®: Now available on your iPhone or BlackBerry http://windowslive.com/RediscoverHotmail?ocid=TXT_TAGLM_WL_HM_Rediscover_Mobile1_042009___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Question: Slides/Day?
Hello Histonetters, I understand most of the professionals on this website are histology professionals, however, I thought I would give it a try since I have not found a cytology listserv yet. Does anyone know how many slides per day a cytotechnologist would screen within a private lab setting (On average)? -- Alyssa Peterson Allied Search Partners O: 770.621.2639 ext. 4 F: 770.621.2640 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Davidson's fixative
Hello Histonetters! I was wondering if any one knows of any studies done on Davidson's fixative and breast IHC. We have been using Davidson's for our colons for about two years now (thanks to the Histonet) and I would like to expand its use to our very fatty breast cases. Our head pathologist is worried that it will affect the IHC. Thank you all in advance. Christine Bark HT(ASCP) CM Lead Histotech, Pathology Saddleback Memorial Medical Center 949-452-3548 cb...@memorialcare.org __ Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] low profile blades and cutting angle
I like both, but use the MX because they are cheaper, and they are, if not quite as good as, certainly a much greater value than the AccuEdge blades (I do think that on average I go through the MX a little faster, but they are still cheaper!!). I alsoo like the MB22 from ThermoFisher for cutting small, thin sections, like renal bx. Bonnie Whitaker Clinical Histology Manager Ohio State University Medical Center N308B Doan Hall 410 W. 10th Ave. Columbus, OH 43210 bonnie.whita...@osumc.edu phone 614.293.5048 fax 614.293.7273 pager 614.346.5013 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Pamela Marcum Sent: Thursday, April 02, 2009 3:42 PM To: Hazel V Horn Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] low profile blades and cutting angle I am with you Hazel. We cut paraffin and today I am cutting GMA plastic with them. They are great for us. Pam Marcum UPENN Vet School New Bolton Center - Original Message - From: Hazel V Horn hor...@archildrens.org To: histonet@lists.utsouthwestern.edu Sent: Thursday, April 2, 2009 3:22:09 PM GMT -05:00 US/Canada Eastern Subject: RE: [Histonet] low profile blades and cutting angle We use MX premier microtome blades and order from ThermoFisher. I do have the accuedge blades but prefer the MX blades. The accuedge blades just don't seem to be as good as they used to be...my opinion only. Hazel Horn Hazel Horn, HT/HTL (ASCP) Supervisor of Histology Arkansas Children's Hospital 1 Children's Way Slot 820 Little Rock, AR 72202 phone 501.364.4240 fax 501.364.3155 visit us on the web at: www.archildrens.org -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Bernice Frederick Sent: Thursday, April 02, 2009 1:20 PM To: 'Merced Leiker'; barricksta...@yahoo.com; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] low profile blades and cutting angle It's all I ever use and I've tried a bunch. Bernice Bernice Frederick HTL (ASCP) Northwestern University Pathology Core Facility ECOGPCO-RL 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Merced Leiker Sent: Thursday, April 02, 2009 12:17 PM To: barricksta...@yahoo.com; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] low profile blades and cutting angle AccuEdge by Sakura Finetek are the best (avail. through VWR) Not only have I tried a few other brands, but our former knowledgeable and experienced histotech here at UB recommends AccuEdge. Merced --On Thursday, April 02, 2009 9:55 AM -0700 Stacey Barrick barricksta...@yahoo.com wrote: Hi everyone, What brand low profile blades does everyone prefer and where do you order them? Also, what blade angle does everyone prefer for cryosectioning? Thanks Stacey ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Merced M Leiker Research Technician II 354 Biomedical Research Building School of Medicine and Biomedical Sciences State University of New York at Buffalo 3435 Main St, Buffalo, NY 14214 Ph: (716) 829-6033 Fx: (716) 829-2725 No trees were harmed in the sending of this email. However, many electrons were severely inconvenienced. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet * * * * * * * * ** The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in
RE: [Histonet] So... I Need Some Expert Advice:
Barry: Agree. Pick up ONE theme and ask for everybody to contribute. If satisfactorily covered, pick another and so on. René J. --- On Thu, 4/2/09, Rittman, Barry R barry.r.ritt...@uth.tmc.edu wrote: From: Rittman, Barry R barry.r.ritt...@uth.tmc.edu Subject: RE: [Histonet] So... I Need Some Expert Advice: To: HISTONET histonet@lists.utsouthwestern.edu Date: Thursday, April 2, 2009, 11:44 AM Might I suggest that what we need on Histonet is to deal with some basic questions in histology that have never been answered in detail but have only been touched upon. Histonet has a great deal of expertise and it seems we should be asking these major questions. Topics such as what is optimal time of fixing, best waxes etc. and why should be issues here. My perception is that we often will give personal opinions as to what the best techniques or materials are but not the science behind the rational for choosing these, maybe because we do not know. However I feel that this is an area that we need to expand. Hope that this makes sense. I've had a week of family medical issues, broken plumbing under the house (guess that better than personal plumbing being broken!), two computer crashes and fire ants to name only the major ones. There is therefore, in my opinion, no way to go but up from here so can we hear your views please? Barry ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] HT licensing in 2011
Laurie, The bill that everyone has been quoting is AB 2156. It does not state that histotechnicians will have to be licensed. The ASCP has specific eligibility requirements for HT certification and at this point they are not related to licensure in any state. Other states that have licensure do not just accept payment for the license. They require some form of competency exam. If a person is already ASCP certified than in some states they will be granted licensure. This is an excerpt from the bill. Under existing law, unlicensed laboratory personnel are authorized to perform specified activities, in a licensed clinical laboratory, under the direct and constant supervision of a physician and surgeon or another licensed person if certain criteria are met. Existing law authorizes these unlicensed laboratory personnel to perform specimen labeling, handling, preservation or fixation, processing or preparation, transportation, and storing. This bill would authorize a certified pathologists' assistant, within the specialty of pathology, demonstrating specified competency, to perform specified activities under the supervision and control of a pathologist. The bill would authorize a pathologists' assistant, a histologic technician, and a histotechnologist to prepare human surgical specimens for gross description and dissection under the direct supervision, as defined, of a qualified pathologist, if he or she meets specified requirements. The bill would authorize the department, on and after January 1, 2011, to adopt regulations establishing additional qualification requirements to perform the duties specified in these provisions. Laurie Colbert laurie.colb...@huntingtonhospital.com Sent by: histonet-boun...@lists.utsouthwestern.edu 04/02/2009 12:38 PM To histonet histo...@pathology.swmed.edu cc Subject [Histonet] HT licensing in 2011 Does anyone know about a law that is supposed to go into effect in 2011 that will require histotechs to be licensed? I also heard something about a person being eligible to take the HT exam if they get a state license - which can be obtained by simply paying a fee if they have 5 years of work experience? Laurie Colbert ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Question: Slides/Day?
Our cytotechs screen 40 to 60 a day. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Alyssa Peterson Sent: Thursday, April 02, 2009 3:49 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Question: Slides/Day? Hello Histonetters, I understand most of the professionals on this website are histology professionals, however, I thought I would give it a try since I have not found a cytology listserv yet. Does anyone know how many slides per day a cytotechnologist would screen within a private lab setting (On average)? -- Alyssa Peterson Allied Search Partners O: 770.621.2639 ext. 4 F: 770.621.2640 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet * CONFIDENTIALITY NOTICE * This message contains confidential information and is intended only for the individual named. If you are not the named addressee you should not disseminate, distribute or copy this e-mail. Please notify the sender immediately by e-mail if you have received this e-mail by mistake and delete this e-mail from your system. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Davidson's fixative
Christine: Unfortunately our profession has no standards with regards of how we do things, we tend to add twists even to recognized procesures, is in our histotechs DNA. Each histolab processes tissues mostly according to its own experience with previous results. So I think that the best for you, instead of relying on others experiences, you should try to test in parallel using some cases that you will process in Davidson and using whatever you are using now, including the IHC. That first hand experience I think will be much more helpful for you. René J. --- On Thu, 4/2/09, Christine Bark cb...@memorialcare.org wrote: From: Christine Bark cb...@memorialcare.org Subject: [Histonet] Davidson's fixative To: histonet@lists.utsouthwestern.edu Date: Thursday, April 2, 2009, 3:50 PM Hello Histonetters! I was wondering if any one knows of any studies done on Davidson's fixative and breast IHC. We have been using Davidson's for our colons for about two years now (thanks to the Histonet) and I would like to expand its use to our very fatty breast cases. Our head pathologist is worried that it will affect the IHC. Thank you all in advance. Christine Bark HT(ASCP) CM Lead Histotech, Pathology Saddleback Memorial Medical Center 949-452-3548 cb...@memorialcare.org __ Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] HT licensing in 2011
Is this a state bill, a federal bill or what. I have never heard of this. And are these specified requirements also part of the bill? Where can we read more about this bill? Mike -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jennifer MacDonald Sent: Thursday, April 02, 2009 3:35 PM To: Laurie Colbert Cc: histonet-boun...@lists.utsouthwestern.edu; histonet Subject: Re: [Histonet] HT licensing in 2011 Laurie, The bill that everyone has been quoting is AB 2156. It does not state that histotechnicians will have to be licensed. The ASCP has specific eligibility requirements for HT certification and at this point they are not related to licensure in any state. Other states that have licensure do not just accept payment for the license. They require some form of competency exam. If a person is already ASCP certified than in some states they will be granted licensure. This is an excerpt from the bill. Under existing law, unlicensed laboratory personnel are authorized to perform specified activities, in a licensed clinical laboratory, under the direct and constant supervision of a physician and surgeon or another licensed person if certain criteria are met. Existing law authorizes these unlicensed laboratory personnel to perform specimen labeling, handling, preservation or fixation, processing or preparation, transportation, and storing. This bill would authorize a certified pathologists' assistant, within the specialty of pathology, demonstrating specified competency, to perform specified activities under the supervision and control of a pathologist. The bill would authorize a pathologists' assistant, a histologic technician, and a histotechnologist to prepare human surgical specimens for gross description and dissection under the direct supervision, as defined, of a qualified pathologist, if he or she meets specified requirements. The bill would authorize the department, on and after January 1, 2011, to adopt regulations establishing additional qualification requirements to perform the duties specified in these provisions. Laurie Colbert laurie.colb...@huntingtonhospital.com Sent by: histonet-boun...@lists.utsouthwestern.edu 04/02/2009 12:38 PM To histonet histo...@pathology.swmed.edu cc Subject [Histonet] HT licensing in 2011 Does anyone know about a law that is supposed to go into effect in 2011 that will require histotechs to be licensed? I also heard something about a person being eligible to take the HT exam if they get a state license - which can be obtained by simply paying a fee if they have 5 years of work experience? Laurie Colbert ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Re: Insect/Arthropod histotechnology
Hi Ana, I work with insects and related organisms. What did you need to know? -Damien Laudier ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Tissue-Tek II Manual
Does anyone have a copy of the Tissue-Tek II manual that they would be willing to share? (PDF preferred, hard-copy graciously accepted) Thanks (in advance), Bernie Ball Duke University A hat should be taken off when you greet a lady and left off for the rest of your life. Nothing looks more stupid than a hat. P.J. O'Rourke ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Strange circles in IHC slides
Hi Looking at the images this appears to be the prozone effect which is caused by the primary antibody concentration being too high and has been well reported in both immunohistochemistry, immunology and serology articles. Do a google serch for prozone phenomenon and you will find an explanation of the theory involved. The classic feature are the circular areas which are unstained and this is reinforced in the images by the amount of background staining in the areas that are stained. Try titrating your primary out and I am sure will eliminate the problem. regards Tony Reilly Chief Scientist Anatomical Pathology Pathology Queensland Level 1, Building 15 Princess Alexandra Hospital Ipswich Rd, Woolloongabba Q 4102 Australia Ph: 07 32402412 Fax:07 32402930 tony_rei...@health.qld.gov.au V. Neubert histonet.nos...@vneubert.com 3/04/2009 2:12 am Hello, I really have some real links to real pictures of real relevance of the real histonet list. Really promised. http://img12.imageshack.us/img12/8513/ts0402162049.jpg http://img13.imageshack.us/img13/6514/ts0402162104.jpg So, has ever anyone experienced sth. like this? My conjugate control (every step except the antibody) was fine, nothing to be seen about DAB and no circles at all. I used Shandon single-use coverplates, sterile buffer, fresh antibody aliquots. Any idea? Thanks, V. Neubert ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This email, including any attachments sent with it, is confidential and for the sole use of the intended recipient(s). This confidentiality is not waived or lost, if you receive it and you are not the intended recipient(s), or if it is transmitted/received in error. Any unauthorised use, alteration, disclosure, distribution or review of this email is strictly prohibited. The information contained in this email, including any attachment sent with it, may be subject to a statutory duty of confidentiality if it relates to health service matters. If you are not the intended recipient(s), or if you have received this email in error, you are asked to immediately notify the sender by telephone collect on Australia +61 1800 198 175 or by return email. You should also delete this email, and any copies, from your computer system network and destroy any hard copies produced. If not an intended recipient of this email, you must not copy, distribute or take any action(s) that relies on it; any form of disclosure, modification, distribution and/or publication of this email is also prohibited. Although Queensland Health takes all reasonable steps to ensure this email does not contain malicious software, Queensland Health does not accept responsibility for the consequences if any person's computer inadvertently suffers any disruption to services, loss of information, harm or is infected with a virus, other malicious computer programme or code that may occur as a consequence of receiving this email. Unless stated otherwise, this email represents only the views of the sender and not the views of the Queensland Government. ** ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
