Re: [aroma.affymetrix] Re: Problem with GLAD on linux cluster

2010-08-02 Thread Henrik Bengtsson
Hi. On Mon, Jul 26, 2010 at 12:00 PM, cstratowa christian.strat...@vie.boehringer-ingelheim.com wrote: Dear Henrik, Maybe, my explanation was not clear enough: I have created my own package based on S4 classes, where one subclass is AromaSNP with slots celset, normset, plmset, effectset as

Re: [aroma.affymetrix] medpolish in analyzing HuGene arrays

2010-08-02 Thread Henrik Bengtsson
Hi Steven. On Mon, Jul 26, 2010 at 9:00 PM, Steven Bosinger steven.bosin...@gmail.com wrote: Hi, I'm new to aroma and bioC in general, so these are probably a very straightforward questions: I am using aroma to get QC on some Human Affymetrix Gene arrays. 1. To keep it consistent with

Re: [aroma.affymetrix] Batch Adjusted RMA data

2010-08-02 Thread Henrik Bengtsson
Hi Fong(?). On Sat, Jul 24, 2010 at 2:10 AM, Fong fongchunc...@gmail.com wrote: Hi, I've used aroma.affymetrix to generate and extract the probeset summaries (chip effects) from a set of Human Exon array samples I have.  And then performed batch adjustment on these probeset summaries using

[aroma.affymetrix] Re: Mo-Ex 1.0st array Analysis using aroma.affymetrix and FIRMA model

2010-08-02 Thread Sundar
Hi, I am trying to analyze Mouse Exon 1.0 st array data using aroma.affymetrix and FIRMA model to find the splicing variants. CDF file : MoEx-1_0-st-v1,coreR1,A20080718,MR.cdf ( downloaded from the aroma.affymetrix website) CEL files: 12 Mouse Exon 1.0 st arrays. ( 6 arrays for each strain

[aroma.affymetrix] non finite values in FIRMA results

2010-08-02 Thread Adi Tarca
Hi all, I have a batch of 107 mice exon arrays for which I computed FIRMA scores and I got many NaN, Inf and 0 values which disable further analysis based on log FIRMA values for some probesets. I was wondering if this is a known issue or I am the only one to get these results. Here is the code

Re: [aroma.affymetrix] Re: Mo-Ex 1.0st array Analysis using aroma.affymetrix and FIRMA model

2010-08-02 Thread Henrik Bengtsson
Hi Sundar, I've been leaving your messages to the FIRMA experts, because they can better answer you questions. However, I'll give a quick reply to the things I can answer. On Mon, Aug 2, 2010 at 6:59 PM, Sundar sundar...@gmail.com wrote: Hi,       I am trying to analyze Mouse Exon 1.0 st

Re: [aroma.affymetrix] non finite values in FIRMA results

2010-08-02 Thread Henrik Bengtsson
Hi, I'll leave the details to FIRMA experts, but you are using really large values of argument 'ram'. It might be that you ran out of memory (the you got an error message). If you used cut'n'paste, instead of source(), to do the analysis, it might be that one of the fit() methods was

[aroma.affymetrix] Re: non finite values in FIRMA results

2010-08-02 Thread Adi Tarca
Hi Henrik, Thanks for your answer.  It might be that you ran out of memory (the you got an error message).  If you used cut'n'paste, instead of source(), to do the analysis, it might be that one of the fit() methods was preemptively finished.  If so, not all units have been fitted.  Rerun