Lepp, Dion wrote:
> Thanks, that was the problem. It is working now.
>
> One quirk/bug I came across is that after re-creating spot images for a
> bioassay, the images from the original creation still remain. Logging
> in and out of Base does not correct this. However, refreshing the
> webpag
To: BASE ML
Subject: Re: [base] spot images error
Lepp, Dion wrote:
> I am uing BASE 2.4.5 (build #3894; schema #40)
>
> Dion
>
> Agriculture and Agri-Food Canada - Agriculture et Agroalimentaire
> Canada
>
> -Original Message-
> From: [EMAIL PROTECTED]
>
s
> Nordborg
> Sent: Wednesday, March 26, 2008 1:16 PM
> To: BASE ML
> Subject: Re: [base] spot images error
>
>
> Lepp, Dion wrote:
>> I'm trying to create spot images from a tiff file (single layer from
>> Scanarray software) and keep receiving an error with th
: Re: [base] spot images error
Lepp, Dion wrote:
> I'm trying to create spot images from a tiff file (single layer from
> Scanarray software) and keep receiving an error with the following
stack
> trace. The image is 5 um, and I'm using the parameters given in the
> .gpr f
Lepp, Dion wrote:
> I’m trying to create spot images from a tiff file (single layer from
> Scanarray software) and keep receiving an error with the following stack
> trace. The image is 5 um, and I’m using the parameters given in the
> .gpr file (xscale= 5, yscale=5, xoffset= 1155, yoffset = 15
I'm trying to create spot images from a tiff file (single layer from Scanarray
software) and keep receiving an error with the following stack trace. The
image is 5 um, and I'm using the parameters given in the .gpr file (xscale= 5,
yscale=5, xoffset= 1155, yoffset = 1577) and a spot size = 32.
david vilanova wrote:
> Hi folks,
> I finally managed to identify the problem. As i said in my previous
> email i tried a tif image with only a signe channel:
>
> [EMAIL PROTECTED] SPOTS]$ ls *
> -rw-r--r-- 1 david bioinfo 9,1M Jan 23 12:19 532.tif
> -rw-r--r-- 1 david bioinfo 9,3M Jan 23 12:20 6
Hi folks,
I finally managed to identify the problem. As i said in my previous email i
tried a tif image with only a signe channel:
[EMAIL PROTECTED] SPOTS]$ ls *
-rw-r--r-- 1 david bioinfo 9,1M Jan 23 12:19 532.tif
-rw-r--r-- 1 david bioinfo 9,3M Jan 23 12:20 635.tif
-rw-r--r-- 1 david bioinfo 2
Thanks for the update
Well still not working, but i tried to see if the problem was coming from
the tiff files.
When I check for the structure of the tif file here is my output:
[EMAIL PROTECTED] Desktop]$ identify image.tif
image.tif[0] TIFF 550x1429 550x1429+0+0 PseudoClass 65536c 16-bit 34
david vilanova wrote:
> Hello,
> I have tried with:
> PixelSize="10"
> ImageOrigin="4440,1000"
> I have double checked the image within genepix and the coordinates
> (expressed in µm) are correct (gpr file matches with the tiff file). 1µm
> correspond to 10pixels in genepix.
I think it is the ot
Hello,
I have tried with:
PixelSize="10"
ImageOrigin="4440,1000"
I have double checked the image within genepix and the coordinates
(expressed in µm) are correct (gpr file matches with the tiff file). 1µm
correspond to 10pixels in genepix.
However the spot image is not the expected one. Still gett
david vilanova wrote:
> Hello,
> Following the advise given to emanuel for the generation of the spots, I
> went through the example but could'nt manage to create the spots.
> Do i have to submit three time the tiff file to create the spots in the
> editor (it's asking for jpeg files°. Anyway the
Hello,
Following the advise given to emanuel for the generation of the spots, I
went through the example but could'nt manage to create the spots.
Do i have to submit three time the tiff file to create the spots in the
editor (it's asking for jpeg files°. Anyway the jpeg files didn't work, i
was ge
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