Re: [base] procedure for obtaining normalized data

2007-09-24 Thread Jari Häkkinen
Hi, I think this is a problem with negative and zero intensity values (due to FG-BG). You need to decide how to treat these intensities and the filter or modify these numbers. After resolving this, you can proceed as you describe. Cheers, Jari Ana Conesa wrote: > Hi > > I am using BASE to

[base] procedure for obtaining normalized data

2007-09-18 Thread Ana Conesa
Hi I am using BASE to store and analyze 2 color (GenePix) data from a costum cDNA array. I am addressing now the analysis of an experiment. I created a raw bioassay out of raw data files and I would like now to normalize. I am not sure which is the standard procedure to do this. At first I calcula