Hi,
I think this is a problem with negative and zero intensity values (due
to FG-BG). You need to decide how to treat these intensities and the
filter or modify these numbers. After resolving this, you can proceed as
you describe.
Cheers,
Jari
Ana Conesa wrote:
> Hi
>
> I am using BASE to
Hi
I am using BASE to store and analyze 2 color (GenePix) data from a
costum cDNA array.
I am addressing now the analysis of an experiment. I created a raw bioassay
out of raw data files and I would like now to normalize. I am not sure
which is the standard procedure to do this. At first I calcula