Hi I am using BASE to store and analyze 2 color (GenePix) data from a costum cDNA array. I am addressing now the analysis of an experiment. I created a raw bioassay out of raw data files and I would like now to normalize. I am not sure which is the standard procedure to do this. At first I calculated FG-BG using the formula intensity calculator and then I tried to compute M values on the result as log2(ch1/ch2) with the idea of running lowess normalization on this result, but this is giving me a "value" error, so I guess this is not the way. Could anyone give me a bit of help on this Thanks a lot Ana
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