Post-doctoral positions are available in the Protein Design group
at Yokohama City University. Projects include beta helical bacterial
virulence factors and protein-based nanocomponents for semiconductor
manufacture (in collaboration with Panasonic). See J. Biol. Chem. 280,
17339-17345 (2005)
1) Why do you think there is twinning? If you label a structure whose
true spacegroup is H32 as having SG H3, then some of the twinning
analyses report that this is consistent with perfect twinning..
I believe the graphs of the moments from TRUNCATE give a true indicator.
Ditto the latest
In addition to trying lower concentrations of PEG and protein, you might
consider whether aggregation in your protein stock or vibration in your
crystallization environment are contributing to the problem, and how
they might be minimized. It might be obvious, but we have found that it
is
I've found that crystallization in sitting drops under oil dramatically reduces
the no. of nucleation events and increases the overall crystal size. Now, if
the increased crystal size helps improve diffraction is something to be tested.
Among the many other suggestions...
Raji
Dear All,
We are working with a DNA binding protein with 4 Zn sites in in ASU. Using SnB
and then CNS we were able to get a map using MAD phasing and could visualize
the density for the double helix of the DNA but it was a left handed spiral
instead of the usual right handed one. The space
Hello Ruchi,
I know that when you use non-crystallographic averaging there is a possibility
of the starting with positive density and ending up with negative, the
enantiomorph, or the negative enantiomorph density. In order to shift back to
the correct phases you can apply a simple formula.
Hi,
If you have density for the protein and the DNA and the density for
the protein is correct and you see left-handed DNA density, then I
suppose you are seeing Z-DNA?
Doesn't seem like a problem of incorrect enantiomorph if your protein
density is fine. You can pull out a Z-DNA structure
