Does anyone has experience in shipping
these compounds
Try Hampton Research.
Christopher J. Law, PhD.,
Skirball Institute,
NYU Medical Center,
New York, NY 10016, U.S.A
On 11/02/2008, David Aragao [EMAIL PROTECTED] wrote:
Dear All,
We need to ship small amounts ( 100ul, [ ] 5mM) of heavy
Hi Jeroen,
We used a BioRad iCycler iQ for the measurements in
Thermofluor-based high-throughput stability optimization of proteins
for
structural studies. Ericsson, et. al. Analytical Biochemistry (2006)
357:289-298.
The wavelengths for excitation and detection were ~490 and ~575 nm,
Hi Jeroen,
I assume you are planning to use Sypro Orange. We are using the Roche LC480
(simply because it's around in the lab next door) and it does the job although
it's a little inconvenient to tweak the settings.
Many people use the iCycler from Biorad. This machine is a little more
You can download the latest FIT2D executables from:
http://ftp.esrf.eu/pub/expg/FIT2D/
Make sure to use the http protocol to download the files, ftp will not work.
The most recent version of FIT2D is for MacOSX. Updates and support for
other OS were apparently discontinued.
Alexander Johs
Edward Berry wrote:
Dirk Kostrewa wrote:
Dear Dean and others,
Peter Zwart gave me a similar reply. This is very interesting
discussion, and I would like to have a somewhat closer look to this to
maybe make things a little bit clearer (please, excuse the general
explanations - this might be
The protocol (ftp://)from the link is wrong, ftp is not supported by the
esrf site (which one is told when actually ftp'ing there. Try
http://ftp.esrf.eu/pub/expg/FIT2D/, it works for me
Tim
--
Tim Gruene
Institut fuer anorganische Chemie
Tammannstr. 4
D-37077 Goettingen
GPG Key ID =
Hi Jeroen,
We just bought a Stratagene Mx3005p for the Thermofluor method (also
known as differential scanning fluorimetry). This was after talking to
Martin, among others, he he... We haven't had it long and did our
first experiments last Friday, but it produced good results straight
I've been trying to get a copy of Fit2D for MacOSX, but the esrf web
site with the executables has been unavailable for some time. The
actual documentation is still online, including mirrors, but not the
link to the executable. The author has also not responded. Anyone
know what's up with
Juergen Bosch and Ben Eisenbraun were quick to forward my request to
Dave Gohara who's at WUSTL now. Dave was equally quick in pointing out
that a universal binary of the USB-to-serial driver resides on his
homepage (http://smackfumaster.com) in the Software - Crystallography
and EM Binaries
On Tue, Feb 12, 2008 at 05:16:14PM +, Andreas Förster wrote:
I've been trying to get SGI dials to work with a MacBook Pro running
Leopard. I can't get my digitus USB-to-serial adaptor to work. The
supplied driver is PPC only. As the chip is from Prolific, I tried the
driver from sbgrid
Dear Colleague:
You and your colleagues are cordially invited to join us for the 13th
Annual Structural Biology Symposium to be held at the University of
Texas Medical Branch at Galveston on May 16th and 17th, 2008. The
meeting is organized by the Sealy Center for Structural Biology
Dale Tronrud wrote:
In summary, this argument depends on two assertions that you can
argue with me about:
1) When a parameter is being used to fit the signal it was designed
for, the resulting model develops predictive power and can lower
both the working and free R. When a
I would recommend giving Dave Ritchie's Hex program a go - it is blisteringly
quick. It's limited to local searches so it is best if you have a reasonable
idea where you expect the docking surface to be (mutations, NMR shift data
etc). We used it to refine solutions from searches with
Hey all,
I've been trying to get SGI dials to work with a MacBook Pro running
Leopard. I can't get my digitus USB-to-serial adaptor to work. The
supplied driver is PPC only. As the chip is from Prolific, I tried the
driver from sbgrid (David Gohara's work). That also seems to be PPC only.
I
Dear All,
A very simple stupid question: is it worth while getting xprep
instead of shelxc to do the same job? i realize xprep does a lot of
other things, but also seem to get the idea from some places that
it does a better job than shelxc at preparing data from shelxd, or is there
any
Sorry for the off-topic but can somebody recommend highly a sensitive
RT-PCR machine for the thermofluor experiment (sypro orange).
That would imply excitation below 500 nm (ideally 470) and detection at
about 570 nm, right? I know several simple machines have a problem with
the 570 nm...
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