Hi again,
Martin pointed out to me that my description of the BioRad machine was
in fact of an older model from ca. 2003 which indeed required manual
filter changes. The iQ5 has multiplexing capability just like the
Mx3005p. I'll leave further discussions to the experts ;-)
Derek
xprep is free to academics. Since your signature says you work at the
University of Helsinki, you would only need to send an informal email to
bruker (you could try their contact form
http://www.bruker-axs.com/contact.html?L=http%3A%2F%2Fhome.arcor.de%2Fjohnwayne92%2Finc.php
) in order to
oops, I am sorry I hit the reply-key at the wrong email. My (anyhow
obsolete) comment refers to the question about fit2D (as you might have
guessed).
Tim
--
Tim Gruene
Institut fuer anorganische Chemie
Tammannstr. 4
D-37077 Goettingen
GPG Key ID = A46BEE1A
On Tue, 12 Feb 2008, Tim Gruene
Hei Jeroen,
Quite recently we got BioRad MiniOpticon system for the thermoflor based
assays. I think this is the least expensive RT-PCR on the market but it performs
very well in our experiments.
It can excite the samples between 470 and 505 nm and one of the detection
channel covers the
Dear all,
a question very related to the discussion before. I have been reading the
papers about the thermofluor experiment with great interest.
I wonder what people think about the underlying principles/ideas and the
success that the method yielded in their own labs for crystallization or
Hi Tim,
Unfortunately your statement is not true.
The XPREP program was written by George Sheldrick over many years and it
is proprietary software distributed by Bruker AXS.
We unbundled it from our complete SHELXTL structure solution and
refinement package, specifically also to serve
Dear Kornelius,
I found the idea of doing Thermofluor on membrane proteins really
intriguing - for identifying the best buffer and detergent, secondary
detergents, even for checking crystallization drops that stayed clear.
(This latter experiment should theoretically be possible with large
Kornelius,
have a look at the following paper:
Yeh AP, McMillan A, Stowell MH.
http://www.ncbi.nlm.nih.gov/pubmed/16552147?ordinalpos=1itool=EntrezSystem2.PEntrez.Pubmed.Pubmed_ResultsPanel.Pubmed_RVDocSum
yes indeed, the Biorad MiniOpticon is the cheapest so far. Next one on
my list is the Applied Biosystems StepOne. Can anybody comment on the
latter machine (not the more expensive StepOnePlus)?
The machines proposed like Roche LC480, Stratagene Mx3005p or Biorad iQ5
are far beyond 30 k€ list
Dear all, deadline for submission of proposals for beamtime at bm14 is set
for next Wednesday (20th Feb 2008) Proposals arriving on this date will be
considered for scheduling in the April/May 2008 synchrotron run at the ESRF.
Beamline proposals can be submitted on-line from the BM14 webpages
XPREP and SHELXC use different algorithms and sometime one of them will
give better results than the other. In particular for MAD data, XPREP
takes the actual f' and f values into account and can refine them if you
have at least three wavelengths, whereas SHELXC uses standard values and
scales
Dear Colleagues,
We are organizing the following EMBO Practical Course:
STRUCTURAL CHARACTERIZATION OF MACROMOLECULAR COMPLEXES
2-7 JUNE 2008GRENOBLE, FRANCE
TOPICS INCLUDE:
- expression and purification of multi-subunit complexes
- biophysical biochemical characterization of
Posted on behalf of Prof. Rolf Hilgenfeld:
Applications are invited for the following positions becoming available
at the Institute of Biochemistry, University of Lübeck (Director: Prof.
Rolf Hilgenfeld).
Deadline for applications: February 29, 2008
Starting date; as soon as possible:
1.
CALL FOR PROPOSALS FOR BEAMTIME WITH ONLINE MICROSPEC
Proposal Deadline *1st March 2008
*There will be beamtime available at the ESRF for MX data collection with
a setup that allows online monitoring of UV/VIS spectral changes of the
crystal during the X-ray diffraction experiment. Users who
Hi,
We are looking for a good and price-effective anti-6His antibody for
detection of protein expressions (western or ELISA). Anyone can give
some suggestions (brand, cat no, etc)? Thank you very much.
Hongmin Li, PhD
Wadsworth Center
New York State Department of Health
IMPORTANT NOTICE:
The Protein Sciences Group of Roche Palo Alto is seeking a Protein
Chemist.
The Position:
The Protein Sciences group at Roche Palo Alto is seeking a Protein
Chemist for purification and characterization of membrane proteins (such
as GPCRs and Ion-Channels). The successful candidates will
Dear CCP4BB members,
we have an opening for a permanent Support Scientist position to
work on the Phase 1 beamlines for Macromolecular Crystallography (MX)
at Diamond Light source. The application deadline is 7th March 2008.
The job reference is DIA0387/SB - Macromolecular Crystallography
Hi Hongmin,
I have used the anti-penta-His HRP conjugate antibody from Qiagen, and it works
very well. It comes as a kit which includes a special blocking buffer. You
don't need to use any secondary antibody, so it saves time. But you can only
use the diluted solution 1-2 times within 1-2
Hi,
I did not see this particular instrument mentioned here so far:
The FluoDia T70 from Photon Technology International, NJ,
http://www.pti-nj.com/PlateReader/PlateReader.pdf
The whole instrument comes with its own thermal cycler and 96-well fluorescence
plate reader with appropriate
I am trying to build and refine a model into 3.6 angstrom Se-met phased
maps. What is the best way to define secondary structure restraints for
refinement? (hydrogen bonds? backbone torsion angles?) Are there any
tools available to help me define restraints for a specified region, or
do I
Hi Sean,
Not an answer to your question but have you looked into model building with
TEXTAL? I am not sure
about 3.6Ang resolution data but it might be worth looking into.
Raji
-Included Message--
Date: 13-feb-2008 16:54:33 -0500
From: Sean Johnson [EMAIL PROTECTED]
To:
For a (very) low resolution RNA/protein complex in which we had high(er)
resolution structures for both components, I used the optimal hydrogen
bonds from these structures (WHATIF output) as restraints. I made a
couple perl scripts to take this output and generated either CNS or
REFMAC
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