[ccp4bb] overlapping DNA in prt-DNA complex structure

2008-03-10 Thread Sabine Schneider
Hello everyone, I am working on the structure of a protein-DNA complex with 2 mol/ASU. Data are to 2.8A and the symmetry appears to be orthorhombic (P212121; a=42.280 b=113.340 c=134.670) I solved the structure by moleculare replacement using the coordinates of the protein in phaser. I got

Re: [ccp4bb] overlapping DNA in prt-DNA complex structure

2008-03-10 Thread James Stroud
Both molecules are bound to the same piece of DNA and the DNA is along the b-axis. Just drop one of the DNA duplexes. James On Mar 10, 2008, at 12:54 AM, Sabine Schneider wrote: Hello everyone, I am working on the structure of a protein-DNA complex with 2 mol/ ASU. Data are to 2.8A and

[ccp4bb]

2008-03-10 Thread Marius Schmidt
Dear Colleagues, A month ago I posted a request to the ccp4bb regarding the malfunction of Xfit under Suse Linux 10.3. I got a lot of suggestions. At least I want to share my experience. 1.) I did exactly that what Paul suggested (see below) installing fedora libX11s wget

[ccp4bb] Staff Position Available: Macromolecular Crystallography Laboratory Manager

2008-03-10 Thread Ann West
Macromolecular Crystallography Laboratory Manager. The University of Oklahoma Department of Chemistry and Biochemistry seeks an individual with a doctorate in Chemistry, Biochemistry, or Physics (or a related relevant field) OR a combination of education and experience (e.g. M.S. degree with

[ccp4bb] topology and parameter file

2008-03-10 Thread Surajit Banerjee
Hi All, Though it is not directly associated with CCP4, but to get an answer I have chosen this path. I am working with a DNA-Protein structure and need a topology-parameter file (CNS) for the modified DNA site. To create the files I am using The Dundee PRODRG2 Server and have a

[ccp4bb] Nucleic acid refinement with Refmac at 3.6A

2008-03-10 Thread Alan Cheung
Hi all - i'm trying to refine a protein-nucleic acid complex at 3.6A resolution with refmac, with weight matrix 0.01. However, after restrained refinement, many of the base pairs are no longer co-planar. Are there watson-crick restraints in refmac, or some other restraint keyword that might

[ccp4bb] Postdoctoral Position in Structural Biology of the Cell Surface Receptors

2008-03-10 Thread 김 경규
Postdoctoral Position in Structural Biology of the Cell Surface Receptors Sungkyunkwan University School of Medicine Samsung Biomedical Research Institute Suwon, Korea A post-doctoral position in structure biology of the cell surface receptors is immediately available at Sungkyunkwan