Re: [ccp4bb] Shredded E coli pellets

Jacob, You should be able to get rid of the problem within a few days as long as everyone in the lab is willing to cooperate. Autoclaving works (it helps to use longer autoclave cycles - 40 minutes instead of the regular 20). If you confirm that phage is your issue - walk through the lab and

Re: [ccp4bb] Shredded E coli pellets

Jacob Before you spend you holiday weekend dephaging the lab and picking up on a comment from Artem and yourself, your protein could indeed be toxic to the cells. You do state in your email that the your are working with new constructs. What happens with your old constructs ? I've been unfortunate

[ccp4bb] Call for applications to the Membrane Protein Laboratory at Diamond

Call for applications to the Membrane Protein Laboratory at Diamond Applications are invited for proposals to use the Membrane Protein Laboratory (MPL) at Diamond Light Source Ltd. Closing date for applications: 28th July, 2009. MPL website: http://www.diamond.ac.uk/Home/MPL.html The MPL

[ccp4bb] Postdoctoral fellow position in Japan...

Replies to kamzh...@riken.jp Postdoctoral Fellow Position in Computational Structural Biology at the Zhang Initiative Research Unit, Advanced Science Institute, RIKEN, Japan We are seeking a highly motivated and experienced computational structural biologist to join the Zhang Initiative

[ccp4bb] Problem with 2 runs in scala

Dear all, I'm trying to scale a dataset where 100 frames in the middle (25 degrees) are quite bad so I would like to exclude them. Because the angular range is too wide I can only do that by creating two runs. So far, so good. The problem is that even if my input contains these five

Re: [ccp4bb] Problem with 2 runs in scala

Thanks to all who answered! Removing RUN 1 reference did the trick Another idea was to simply: EXCLUDE batch 551 to 650 but then, smoothed scaling is not possible (to wide a gap ) Best regards, Miguel 2009/7/3 Miguel Ortiz Lombardia miguel.ortiz-lombar...@afmb.univ-mrs.fr : Dear all,

[ccp4bb] Equipment

Posted on behalf of Wolfram Saenger: X-ray equipment to give away Since I am close to retirement, the equipment I have will be given away at no cost except for transport. The equipment are two mar research image plates, 345 and 250 mm diameter, the first running under PC, the secondhas an

Re: [ccp4bb] can I try crystallization in high salt?

In short, yes you can. Although it is often avoided due to the limitations that high salt samples may have, you still have a good chance to crystallize the protein in the original high salt buffer. In my case a small alcohol was the best precipitating agent. As with most proteins, try it, try it,

[ccp4bb] CCP4 / Ubuntu 9.04 64-bit

Hi, I just installed the 64-bit version of Ubuntu 9.04 Desktop, and then I installed CCP4 6.1.1 using the standard package and installation script. I can run ccp4i successfully. However, when I try to run Coot, I get a bunch of errors. I think the problem is that Coot was compiled with

[ccp4bb] Why NCS doesn't help?

Dear All, In refining my structure (two molecules in au) that was solved with molecular replacement, refinement starting with restrained lowered the R-free to about 0.35 while with NCS increased the R-free to 0.55. Does it imply that two molecules are quite different or something wrong with

Re: [ccp4bb] How to express a 95KD FAD protein

try codon optimisation use Rosetta 2 strains fuse to MBP I expressed a yeast protein of ~100 KDa, expression was low until I fused it to MBp (resulting protein ~ 150 KDa)