[ccp4bb] Post doctoral position at the Institute of Cancer Research, London

Post Doctoral Training Fellow - David Barford's team - Section of Structural Biology - Institute of Cancer Research - London Applications are invited for a post-doctoral training fellow to join a team investigating the structure and mechanism of proteins that regulate the cell cycle and signal

[ccp4bb] PDB in in Refmac5

hi all, I have a question, what is the input PDB file in Refmac? Is it edited from model building from initial map generated by Phaser(in MR)? What can I do if the initial map is hard to build in? Thanks! Antonio

Re: [ccp4bb] PDB in in Refmac5

You need to give a bit more detail.. Here is a plan for MR but you may not have followed it.. there are many ways to kill a pig! 1) get sequence alignment between model and your sequence in astandard format. 2) run chainsaw to edit the model to renumber and rename to your sequence, truncate

Re: [ccp4bb] Alignment of Electron Density Map for structures that have different space groups

Milya Davlieva wrote: Dear all, I need to make a figure which needs to consist of 2 aligned pdb files and their electron density maps for the structures of two mutants (with different space groups). I'm able to do this when there are two structures that have the same space group. Could, you,

Re: [ccp4bb] Alignment of Electron Density Map for structures that have different space groups

Milya Davlieva wrote: Dear all, I need to make a figure which needs to consist of 2 aligned pdb files and their electron density maps for the structures of two mutants (with different space groups). I'm able to do this when there are two structures that have the same space group. Could, you,

Re: [ccp4bb] Rfactor got stuck with a data having alternate strong and weak reflections.

Ben Ammar Youssef wrote: Hi all, I just want to ask another question related to this topic: Based on the example given by Jerry McCully and if the data has alternate strong and weak reflections, how can we split it into two different files; one containing the weak reflections and the other

Re: [ccp4bb] anaerobic crystallization

Chris Although generally less popular nowadays, microbatch-under-oil has great advantages for anaerobic work. You can keep your stock solutions in the chamber all the time, so you only need to degas your protein. Microbatch finds roughly as many crystals in screening experiments as vapor

Re: [ccp4bb] stabilizing solution

James Generally you can use the reservoir solution to make your seed stocks, as is routinely done with the MMS microseeding method. However, the seeds will not be completely stable because there will not be much protein in the solution, so you must keep your seed stocks on ice, and freeze

Re: [ccp4bb] How to get rid of the skin in the screening drop

The plates I used were from Hampton, 72 well microbatch plate, untreated hydrophobic ones, HR3-087. I thought about other plates once. But what I had in mind was the thing matters would be the oil since the skin appeared at the interface. So I tried different oils for the plates, including

[ccp4bb] Alignment of Electron Density Map for structures that have different space groups

Dear all, Thank you for your replies, I actually saw the options in coot that Chris and others suggested and I already tried it before, but for some reason it did not work for me. Maybe I`m doing something wrong. So I open in coot pdb1 and map1 for mutant 1 and pbd2 and map2 for mutant 2.

[ccp4bb] CALL FOR PROPOSALS FOR BEAMTIME WITH ONLINE MICROSPEC 30th, 31st October and 1st, 2nd November 2009]

- CALL FOR PROPOSALS FOR BEAMTIME WITH ONLINE MICROSPEC Proposal Deadline 26th September 2009 There will be beamtime available at the ESRF for MX data collection with a setup that allows online monitoring of UV/VIS spectral changes of the crystal during the