Dear CCP4BB members
Sorry for off topic, i had question related to dispersity of proteins of my
interest, i am working on DNA binding protein, having PI around 9 to
10,Initially protein was remaining stable at high salt, but was not able to
concentrate more than 1mg/ml, after using 50mm Arg, 50mm
Dear colleagues,
This is a follow up to our earlier message, requesting prediction
targets for CASP10.
First THANK YOU to those who came forward with targets -- really much
appreciated, and has helped get this CASP off to a great start. We now
have over 70 targets released.
Second, please
Add DNA?
Also, polydispersity is not great for crystallization but may not be
the end of the world :)
Artem
On Mon, Jun 18, 2012 at 4:52 AM, Arpit Mishra ar...@igib.in wrote:
Dear CCP4BB members
Sorry for off topic, i had question related to dispersity of proteins of my
interest, i am
Well - I guess you need to check the input mtz file.
do mtzdmo this.mtz and check that the columns you have selected are present and
have non-zero values. You need a FOM there.
Eleanor
On 14 Jun 2012, at 12:58, Appu kumar wrote:
Hello Dear all
I am trying running
Wow, that's very cool! Can you divulge what the function of the
protein is? One thinks of some kind of mechanical spring...
JPK
On Mon, Jun 18, 2012 at 8:49 AM, anna anna marmottalb...@gmail.com wrote:
Hi all!
I'd like your opinion about a structure I solved.
Apart from protein structure
Certainly it's interesting, but I think your description is inaccurate.
Endless linear polymers - Each monomer is a polymer, but a collection
of monomers is called a multimer, not a polymer.
I don't suppose there are any knots? That would be really interesting.
On 06/18/12 09:49, anna anna
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of monomers is called a multimer, not a polymer.
[...]
shiver - what a terrible mixture of languages. 'multi-' has got latin
origin, whereas both poly and mer have got greek origin, and I don't
think one should mix these. Please!!! think of a
how about greek-protomers-bands (aka GPB) :-)
Nice picture, you can make decorative art with it and sell it.
Jürgen
On Jun 18, 2012, at 10:43 AM, Tim Gruene wrote:
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of monomers is called a multimer, not a polymer.
[...]
shiver - what a
On 06/18/12 10:43, Tim Gruene wrote:
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of monomers is called a multimer, not a polymer.
[...]
shiver - what a terrible mixture of languages. 'multi-' has got latin
origin, whereas both poly and mer have got greek origin, and I don't
think one
Try adding DNA then dialyzing to low salt (in some microdialyer).
=
Phoebe A. Rice
Dept. of Biochemistry Molecular Biology
The University of Chicago
phone 773 834 1723
James,
The IUCr report that Ethan referred to distinguishes carefully between
a symmetry element and a symmetry operation. A symmetry element
corresponds to a set of coaxial rotation or screw axes (assuming we're
limiting the discussion to enantiomorphic space groups) in a unit cell
related by
I have been curious and suspicious for a long time about multimer: I
always assumed it to be a more homey substitute for oligomer, as
there seems to me to be no difference in usage, and certainly not in
the etymological sense. I have often heard it used by non-experts who
don't know exactly the
Of course, oligomer (pure Greek) usually does that kind of job, but not in
this specific case, since oligo means few and in this case we have endless
chains.
I can only think of the neologism myriomer for this particular case, if
you want to stick to Greek. Myrioi can mean 1 or countless,
On 18/06/2012, Tim Gruene t...@shelx.uni-ac.gwdg.de wrote:
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of monomers is called a multimer, not a polymer.
[...]
shiver - what a terrible mixture of languages. 'multi-' has got latin
origin, whereas both poly and mer have got greek
On 06/18/12 11:17, Jacob Keller wrote:
But anyway, what is
wrong with calling her structures polymers? Is there a subtle
covalent insinuation to polymer?
subtle? No, it's not subtle.
--
===
All Things Serve the Beam
I love myriomer, but what's wrong with boring old polymer?
JPK
On Mon, Jun 18, 2012 at 10:27 AM, Emmanuel Saridakis
esari...@chem.demokritos.gr wrote:
Of course, oligomer (pure Greek) usually does that kind of job, but not in
this specific case, since oligo means few and in this case we have
Okay, I wiki'd it, and according to them seems you're right: it says
they are typically connected by covalent chemical bonds. So either
we revert to the etymological use of polymer, or move onward to
myriomer! (assuming the cross-bred multimer is out of the
question!)
JPK
On Mon, Jun 18, 2012 at
isn't a polymer considered a poly-multimer of undefined size ?
And you use multi once you run out with your greek naming scheme say when
icosahedron ?
Jürgen
P.S. where are all those greeks to shed some light on us ?
On Jun 18, 2012, at 11:48 AM, Jacob Keller wrote:
Okay, I wiki'd it, and
If the original poster could engineer a few disulfides or other covalent
linkages in there, I would drop my objections, and be even more impressed.
On 06/18/12 11:48, Jacob Keller wrote:
Okay, I wiki'd it, and according to them seems you're right: it says
they are typically connected by
If we are to strictly adhere to polymer as describing few, then were do
we stand with DNA/RNA being described as a polymer - a long polymer made up
from repeating units of nucleotides, as has been used in textbooks for
ages?! Is DNA/RNA too now a myriomer?
Cale
Hi Anna,
Interesting assembly. What is the function of your protein? Is it known if
your protein forms a fibril-like assembly in solution? Moreover, can your
crystal packing be indexed higher symmetry space group?
Cheers,
Shiva
On Mon, Jun 18, 2012 at 9:03 AM, David Schuller dj...@cornell.edu
2012/6/18 Tim Gruene t...@shelx.uni-ac.gwdg.de:
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of monomers is called a multimer, not a polymer.
[...]
shiver - what a terrible mixture of languages. 'multi-' has got latin
origin, whereas both poly and mer have got greek origin, and I
This may be somethng similar
Domain swapping of a llama VHH domain builds a crystal-wide beta-sheet
structure.
Spinelli S, Desmyter A, Frenken L, Verrips T, Tegoni M, Cambillau C.
FEBS Lett. 2004 Apr 23;564(1-2):35-40.
Remy Loris
Vrije Universiteit Brussel
On 18/06/12 15:49, anna anna wrote:
I'm further racking my brain to figure out a biological implication of this
behaviour, I thought something like plaque formation but I can't find support
in literature.
There are a variety of domain swapped crystal structures out there, but at
least the two I'm most familiar with are
I'm further racking my brain to figure out a biological implication of this
behaviour, I thought something like plaque formation but I can't find
support in literature.
A good example of domain swaps involved in disease-associated
polymerisation is the polymerisation of serpins; while
On Monday, June 18, 2012 02:06:46 pm Alexander Scouras wrote:
I'm further racking my brain to figure out a biological implication of this
behaviour, I thought something like plaque formation but I can't find
support in literature.
There are a variety of domain swapped crystal
Hi Anna,
Your structure and description remind me very much of our domain-swapped
crystal structure of trp repressor, also an infinite lattice.
One more example to add to the many that have been pointed out by others.
http://www.pdb.org/pdb/explore.do?structureId=1MI7
Cheers,
Cathy Lawson
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