Hello
could any suggest some ways/tools to calculate average alpha helical rise per
turn for a helical model from PDB.
Thank you in advance
regards
uday
Hi folks,
After a brief hiatus (okay, more than four and a half years) the CCP4
Newsletter on Protein Crystallography is back!
The latest issue is now available from the website
http://www.ccp4.ac.uk/newsletters.php in both HTML and PDF. This edition
follows hot on the heels of the 6.3.0 release
Dear All;
We are planning to establish stable CHO or 293 cell clones to express
some receptors.
I heard that it is time consuming to select high expression clones
using pcDNA3.1 vectors.
As our crystallographer are dealing with all kinds of proteins, can
anyone
Hi Jerry,
Perhaps this Nature protocols paper from the Stroud Lab at UCSF might be useful.
Overexpressing human membrane proteins in stably transfected and clonal human
embryonic kidney 293S cells
http://www.nature.com/nprot/journal/v7/n3/full/nprot.2011.453.html
-john
On Aug 17, 2012,
Quite common. And it may be a good sign for crystallizability in those
conditions. As the concentration gradients dissipate from the initially
mixed drop, your protein re-solubilizes.
Roger Rowlett
On Aug 17, 2012 5:31 PM, Yuri Pompeu yuri.pom...@ufl.edu wrote:
Hello everyone,
I have just made
