A very nice picture of Prof. Louise Johnson can be found on
http://www.flickr.com/photos/wellcomeimages/5814718414/
The picture was taken at Diamond's IO2 beam line.
Condolences to her family and friends.
Fred.
Hi there,
acceptable deviations from ideal geometry depends on the resolution of
the data set. There are several papers dealing with this, including the
paper describing the tool used in Phenix.
I couldn't find the resolution of the data set used during refinement in
your mail so it is
Thanx everybody for your nice suggestions..
On Wed, Oct 3, 2012 at 3:19 AM, Faisal Tarique faisaltari...@gmail.comwrote:
Dear all
i request you to please answer my basic query about the ideal acceptable
rmsbond length obtained during refmac refinement..is the data acceptable in
mine case
Dear Faisal -
There are a few problems with your refinement.
First and foremost, your R factors increase instead of decreasing! This
is associated with a decrease in geometry rmsd, suggesting that Refmac
is trying to fix an incorrect model using an excessively tight geometry
weight.
Professor Dame Louise Johnson was my thesis supervisor and I am saddened by
her departure.
I would like to encourage the crystallographic community to contribute to:
http://en.wikipedia.org/wiki/Louise_Johnson
so that her achievemens can be remenbered and can continue to inspire
future
A postdoctoral position is available for structural and functional
analysis of proteins involved in virulence and/or survival in pathogenic
bacteria. We are particularly interested in developing drugs and
vaccines that target multidrug resistant bacterial strains. See recent
examples of our
Hi all,
I recently got some hits from a Mosquito grown at 18C with PEG4000 and 10%
propanol. I've tried reproducing these hits using the standard 24 well format
with no success, even with playing with PEG and propanol concentrations. The
initial screening solution was from a kit that's been
Peter
We make a 96-well plate that was originally made for microbatch-under-oil,
which has a large moat or well around the whole plate.
My old friend Lesley Haire had a lot of success with this plate for a
condition that contained isopropanol.
She set up the drops without isoprop, covered them
Doesn't seem like there'd have been much isopropanol left in that tube
after 2 years. In fact, your PEG may have gone off as well, changing
the pH and god knows what else. Try screening pH and leaving out
isopropanol in your optimization.
phx
On 3 October 2012 17:37, Peter Hsu
