Hello David,
I would use the SAD target function of refmac5 for the anomalous
occupancy. As of isomorphous occupancy and phasing power, I don't know.
Best,
Tim
On 12/15/2013 10:29 PM, David Schuller wrote:
I have some SIRAS data of a known structure. I want to get the
isomorphous and
Dear Bonsor,
I fully second James suggestions but have a few additional comments:
If you get a solution in P6522 with one molecule, you should get the same
solution in P65 with 2 molecules. One of the crystallographic symmetry
operators would then be non-crystallographic.
The current version of
Dear structural biologists,
there are two positions for postdoctoral researchers available with an emphasis
on high-resolution cryo-EM at OIST (Okinawa Institute of Science and Technology
Graduate University, Japan).
We are a highly motivated young international team and benefit from an
Looks like you need to search for more molecules.
Best,
Herman
Von: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] Im Auftrag von Amanda
Blythe
Gesendet: Montag, 16. Dezember 2013 11:46
An: CCP4BB@JISCMAIL.AC.UK
Betreff: [ccp4bb] Phaser output problem
I am trying to solve a structure by
Things to check - number of molecules to search for.
You can use Matthewscoeff to get a suggestion - MOLREP does it
autromatically .
Space group - has Phaser chosen as best SG an alternative to that in your
header?
Eleanor
On 16 December 2013 11:03, herman.schreu...@sanofi.com wrote:
Looks
I would find the sites from the PHIC - you need to use CAD to add
Fcalc PHIC and FOM to the original data with Fnative Fderiv DANOderiv
etc
I usually then use SCALEIT to scale native and derivative to Fcalc -
then you know you are roughly on an absolute scale
Then feed those sites into Phaser_EP
The Industrial Special Interest Group and Young Scientists Special Interest
Group of the American Crystallographic Association will be hosting a scientific
session on Sunday, May 25, 2014 during the ACA Meeting in Albuquerque, New
Mexico which will highlight research by young scientists
Dear all,
I am trying to use CAD to apply b-factor sharpening to my 3.7 A data.
Following is the script which I am running.
cad hklin1 test.mtz hklout test_out.mtz END-cad
RESOLUTION OVERALL 40 3.7
scale file_number 1 1 -50
END
END-cad
test.mtz is the refmac output.
The output fails to open
Hi,
It looks like you're missing your LABIN line.
Pete
Ashu Kumar wrote:
Dear all,
I am trying to use CAD to apply b-factor sharpening to my 3.7 A data.
Following is the script which I am running.
cad hklin1 test.mtz hklout test_out.mtz END-cad
RESOLUTION OVERALL 40 3.7
scale file_number 1
Dear Uli,
On Mon, 2013-12-16 at 15:52 +, ulrich.goh...@mdc-berlin.de wrote:
Dear colleagues,
Trying to run the latest version of ccp4 (6.4.0), say Refmac, I get
the following error when I fill in the file box for the data file:
CCP4i encountered an error when trying to extract the
Dear Jie,
Thanks for spotting this problem. I've made a fix for MrBUMP that should
address the problem. It will be included in the next CCP4 update which
will go out tomorrow (Tuesday 17th of December) all being well.
Best wishes,
Ronan
On 10/12/13 01:34, jie liu wrote:
Dear All
I just
Yes Uli. I have the same issue, but not solve it yet :-(
Partha
On Mon, Dec 16, 2013 at 10:52 AM, ulrich.goh...@mdc-berlin.de
ulrich.goh...@mdc-berlin.de wrote:
Dear colleagues,
Trying to run the latest version of ccp4 (6.4.0), say Refmac, I get the
following error when I fill in the
Didn't mlphare use to print those values in the log file ?
Jürgen
On Dec 15, 2013, at 4:29 PM, David Schuller
dj...@cornell.edumailto:dj...@cornell.edu wrote:
I have some SIRAS data of a known structure. I want to get the
isomorphous and anomalous occupancy and phasing power from my data.
Dear all, sorry if this topic does not interest you. I wonder whether anyone
has experience with freezing crystals grown in ~0.2 M Magnesium Formate. Garman
and Mitchell suggested that A major anomaly is solution 44, 0.2 M magnesium
formate, which requires 50% glycerol for cryoprotection in
Junyu,
I haven't tried it personally with this particular solution, but I have
found that 30% glucose can pretty much cryoprotect any condition I have
tried it with. If necessary, add cryoprotectant solution (mother liquor
+ 30% glucose) gradually to minimize osmotic shock and potential
On Mon, Dec 16, 2013 at 1:36 PM, Xiao, Junyu jx...@mail.ucsd.edu wrote:
Dear all, sorry if this topic does not interest you. I wonder whether
anyone has experience with freezing crystals grown in ~0.2 M Magnesium
Formate. Garman and Mitchell suggested that A major anomaly is solution
44, 0.2
Does anyone know of a good way to phosphorylate the Tyr on a protein for
structural studies? Is there a generic kinase that can be coexpressed or
purified for phosphorylation?
The pCMF Amber codon system is very expensive
and Glu really doesn't mimic pTyr all that well.
Any ideas/help would be
Dear all
I just installed CCP4 6.4 on Ubuntu 12.1. The program can work. I found it
just used 1 processor instead of 4( Intel i5) for data solving. Does anyone
know how to config CPU so that all processors can be used.
Thanks
Chang
Dear all
I just installed CCP4 6.4 on Ubuntu 12.1. The program can work. I found it
just used 1 processor instead of 4( Intel i5) for data solving. Does anyone
know how to config CPU so that all processors can be used.
Thanks
Chang
19 matches
Mail list logo
