[ccp4bb] Postdoctoral position availiable at the University of Massachusetts

Hi all, An NIH-funded postdoctoral position is available in the Department of Biochemistry and Molecular Pharmacology at the University of Massachusetts Medical School in Worcester, MA. Our multidisciplinary lab focuses on using biochemical, structural, cell biological, microscopy and genetic s

[ccp4bb] Fedora Core 20 issues

Hi all, So, I know this probably isn’t the right place, but I’m certain most of you here have battled/fixed this issue. I just did a clean install of FC20 on an AMD dual core system. I opted for the 32 bit version, as these workstations are used for multiple things (and I find that it’s just

Re: [ccp4bb] frozen pellet insoluble protein

I have experience with some proteins that don't tolerate freeze-thawing very well. It's hard to say exactly what the physical chemistry of this is, but it probably relates to (1) aggregation due to high concentration or protein or salts during the freezing process as water is removed, and/or (2

[ccp4bb] frozen pellet insoluble protein

Dear all, I've encountered people who refuse to freeze cells and always lyse fresh pellets. Better protein, they say. I've never had reason to do so myself, or even to believe in their voodoo. Up until now, maybe. My protein expresses well and is almost all in the soluble fraction in an e

[ccp4bb] Pipettes

Hi, This is way off topic but relevant. Does anyone routinely use the Rainin Classic Pipet series? If so, what are your thoughts and how often do they need to be calibrated? Best wishes, Reza Reza Khayat, PhD Assistant Professor The City College of New York Department of Chemistry, MR-1135 160

Re: [ccp4bb] XDS

Hi, Also, if you go from XDS to aimless with the INTEGRATE_ASCII.HKL or  XDS_ASCII.HKL file, aimless has an option to omit batches (frames) as you wish from scaling or merging.           Boaz     Boaz Shaanan, Ph.D. Dept. of Life Sci

[ccp4bb] AW: [ccp4bb] XDS

Dear Almudena, Doing two different runs (1-900 and 1001-1200) will do what you want. You will have to do it from 2 different directories (called e.g. wedge1 and wedge2, or whatever). With XSCALE you can merge the runs. Best, Herman Von: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] Im Auft

Re: [ccp4bb] XDS

Hi,  Another option to do it in xds (following advice of a Kay) is to rename the frames you don't like differently than the template.  Boaz Original message From: Almudena Ponce Salvatierra Date: To: CCP4BB@JISCMAIL.AC.UK Subject: [ccp4bb] XDS Dear

Re: [ccp4bb] XDS

Hi Almudena, Greeting from Oulu.Other possibility would be, process the group of Frames separately in XDS and merge them in the end. Thank you Rajesh On Mon, Sep 29, 2014 at 12:56 PM, Almudena Ponce Salvatierra < maps.fa...@gmail.com> wrote: > Dear all, > > I would like to ask something

Re: [ccp4bb] XDS

Hi Almudena, if you run XDS through xia2, you can define multiple wedges in your info: BEGIN SWEEP SWEEP1 IMAGE image_0001.cbf DIRECTORY /path/to/files/ START_END 1 900 END SWEEP BEGIN SWEEP SWEEP2 IMAGE image_0001.cbf DIRECTORY /path/to/files/ START_END 1001 1200 END SWEEP Andreas On 29/

[ccp4bb] XDS

Dear all, I would like to ask something regarding XDS. Is it possible, without changing the Name of the Frames, to leave some out while processing? i.e. something like defining twice the data range DATA_RANGE= 1 900 !DATA_RANGE= 901 1000 DATA_RANGE= 1001 1200 Is there a way to do so? to leave ou