Re: [ccp4bb] The predicted unit cell is large and all the molecules will be around the longest axis.

2015-04-26 Thread Edward A. Berry
Also collect while rotating around the long axis, which you nearly are in this shot assuming the Phi axis is horizontal. That way the close separation is in x,y where you can see it rather than in Z which puts the spots on top of each other requiring thin slicing and (low mosaicity!) to

[ccp4bb] Postdoctoral position in Structural Biology - Bilbao (Spain)

2015-04-26 Thread David albesa-jove
A 2-years funded postdoctoral research position is immediately available at the newly established Structural Biology Group, at the Unit of Biophysics (UB), University of the Basque Country (UPV/EHU), Spain. We are seeking a highly motivated individual, interested to investigate the structural,

Re: [ccp4bb] Thin plate crystals

2015-04-26 Thread Prerana G.
Thanks a lot for your suggestions. Regards, Prerana On Fri, Apr 24, 2015 at 7:05 PM, Tanner, John J. tanne...@missouri.edu wrote: Following up on Dave's suggestion, you could try using crystal screens as additives. This has worked well in my lab. The idea is to mix the condition that you

Re: [ccp4bb] Thin plate crystals

2015-04-26 Thread Birtley, James
A reference that describes what Jack is referring to is detailed here: http://www.ncbi.nlm.nih.gov/pubmed/15858279 I have used this technique to improve every protein I have ever crystallised. In the most extreme cases poorly diffracting hopeless crystals that were also strongly anisotropic