Re: [ccp4bb] secondary structure prediction

Thanks for many advices. I was not clear in the previous email. I know the close homologous protein (20% identity, total 500aa), but the fragment hits (30~40aa, identity 40~50%) are from other proteins in PDB. I am trying to see whether the fragments from non-homologous proteins may help the

Re: [ccp4bb] How to find the Rfree and R in the highest resolution bin in Refmac 5?

You can find these numbers in the header of the output model coordinate file. BR From: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] On Behalf Of Xiao Lei Sent: Wednesday, December 6, 2017 2:40 PM To: CCP4BB@JISCMAIL.AC.UK Subject: [ccp4bb] How to find the Rfree and R in the highest

[ccp4bb] How to find the Rfree and R in the highest resolution bin in Refmac 5?

Dear All, I am asking how to find the Rfree and R in the highest resolution bin in Refmac 5 output? I used Refmac5 in refinement of protein structure at 3A resolution. Output of Refmac5 gives Rfee and R as: InitialFinal R factor0.2900 0.2052

Re: [ccp4bb] secondary structure prediction

Modeller Sophia Missoury - Mail original - De: "Smith Liu" À: CCP4BB@JISCMAIL.AC.UK Envoyé: Mercredi 6 Décembre 2017 16:04:12 Objet: Re: [ccp4bb] secondary structure prediction Rosetta Smith Liu

Re: [ccp4bb] secondary structure prediction

I would try your local bioinformatician (or a remote one if there isn't any one local). With the sequence in the mail, I could have given it a shot... Gert On 12/6/2017 3:14 PM, zheng zhou wrote: Dear CCP4 community, Sorry for the off-topic question. I am trying to design constructs for

Re: [ccp4bb] secondary structure prediction

Dear Zheng, You might want to try the tools at: https://ccd.rhpc.nki.nl Tassos On Dec 6, 2017, at 15:14, zheng zhou > wrote: Dear CCP4 community, Sorry for the off-topic question. I am trying to design constructs for structure studies. It

Re: [ccp4bb] secondary structure prediction

Rosetta | | Smith Liu | | 邮箱：smith_liu...@163.com | 签名由 网易邮箱大师 定制 在2017年12月06日 22:14，zheng zhou 写道： Dear CCP4 community, Sorry for the off-topic question. I am trying to design constructs for structure studies. It only has a homolog structure in PDB with sequence identity ~20%. When I

[ccp4bb] secondary structure prediction

Dear CCP4 community, Sorry for the off-topic question. I am trying to design constructs for structure studies. It only has a homolog structure in PDB with sequence identity ~20%. When I blast against PDB sequence, there are quite a few motif hits (30~40aa, identity 40~50%). Any prediction tools

[ccp4bb] Postdoc position at Pasteur Institute, Paris

Dear all, Please, find below an ad for a Postdoctoral position in the Reyes' laboratory at Pasteur Institute in Paris to work on human transporters. Best regards, Nico A Postdoctoral position is available in the Laboratory of Nico Reyes at the Pasteur Institute in Paris for initial 2-3 years