Hello Jiyuan,
One small point to note- as Artem says, small molecule crystals are often
generated out of solvents and these same solvents often melt the standard
protein crystallisation plates, so be careful what you put into a plastic plate.
As Artem mentioned, synchrotrons are generally
Hi
A small organic molecule is typically crystallized from organic solvents
(or water, if soluble) by means of at least three main techniques:
1. slow evaporation of solvent leading to supersaturation and eventual
crystallization
2. supersaturation at higher temperature followed by gradual drop
Hi Everyone,
I want to crystallize a small organic molecule. I have very limited
experience in small molecule crystallography. I found that the Crystal
Screen HT from the Hampton research is good for both small molecule and
macromolecule crystallization. Plan to set up a sitting drop screen just
Good evening,
since I've been contacted off line several times about this, I'm posting
here the protocol to combine maps with 2 mtz files of original and
truncated data.
I went through this procedure as I wanted to include the maps, and this
was the tricky part of the procedure in my hands.
Dear all,
On Sat, May 30, 2020 at 03:40:53PM +0100, Eleanor Dodson wrote:
> My pennysworth. If you find your maps look better after the
> anisotroy correction use it, but it may be helpful to those wo want to mine
> your data if you deposit the whole sphere..
Agree (which is what e.g. we provide
Hey Suchi,
you could firstly increase the binning to factor to 4-5. Also, it would help to
shrink the mask radius. Most definitely keep the resolution limited to 8-10 Å!
Cheers
Schara
Dr. Schara Safarian
Max Planck Institut für Biophysik
Abteilung für Molekulare Membranbiologie
Max Planck
