Re: [ccp4bb] Design of SUMO fusion proteins

 Hi, I would like ask your advice on designing SUMO fusion proteins. Specifically, is it recommended to include a linker between the C-terminus of SUMO (-QIGG) and the N-terminus of the target protein? Figure 3 of Guerrero et al. (https://pubmed.ncbi.nlm.nih.gov/26297996/

Re: [ccp4bb] Design of SUMO fusion proteins

Hi Jack, We routinely include a single glycine downstream of the SUMO (so, …QIGGG). This essentially always gives good cleavage with UD, assuming the construct isn’t aggregated or otherwise unhappy. Re the His tag: Presumably you mean at the N-terminus of SUMO? In this case we’ve had good

[ccp4bb] Postdoc Opportunity at Harvard Medical School

Dear All, A postdoctoral position is available immediately in the laboratory of James Chou at Harvard Medical School. The research project investigates the structural and physical basis of immune receptor autoinhibition and activation. The goal is to use the knowledge to guide the development

[ccp4bb] Design of SUMO fusion proteins

Sorry for the non-CCP4 question… I would like ask your advice on designing SUMO fusion proteins. Specifically, is it recommended to include a linker between the C-terminus of SUMO (-QIGG) and the N-terminus of the target protein? Figure 3 of Guerrero et al.

[ccp4bb] CryoEM Current Practices webinar this week, Thurs April 26

Join the national cryoEM service centers for our next “CryoEM Current Practices” webinar this Thursday: Title: Cryo-EM studies of a malleable oligomeric SF3 helicase Speaker: Rahul Jaiswal, Ph.D., Instructor, Virginia Commonwealth University School of Medicine Time: Thursday, April 29, 2021 at

[ccp4bb] Upcoming Instruct-ERIC Events

[Instruct-ERIC logo] [Instruct-ERIC] [LinkedIn] [Twitter] Dear All Instruct-ERIC and partners are offering a variety of webinars, conferences

[ccp4bb] Online Course on Neutron Scattering Applications in Structural Biology (HANDS2021)

ORNL Online Course on Neutron Scattering Applications in Structural Biology: https://conference.sns.gov/event/291/ Lectures: June 21 - June 25, 2021 Tutorials: June 28 - 30, 2021 2:00 pm - 5:30 pm EST Registration (free) is now open and will close May 14, 2021:

Re: [ccp4bb] solving difficult protein structure

Hi Swati, I am very far from expert, but if you have anisotropic diffraction have you thought of bringing in Staraniso - public server at https://staraniso.globalphasing.org? That should help keeping reflections with higher I/sigma and rejecting those with mostly noise. Yours, Rasmus On