[ccp4bb] Off-topic - Domain termini for construct design

of interest when it is not important to keep them true (=native) in order to optimise expression levels. Is there any good reference about this, please? Personal experiences are welcome. Many thanks, Claudia Claudia Scotti Dipartimento di Medicina Sperimentale Sezione di Patologia

[ccp4bb] Freezing under oil

and suggestions are welcome. Claudia Claudia Scotti Dipartimento di Medicina Sperimentale Sezione di Patologia Generale Universita' di Pavia Piazza Botta, 10 27100 Pavia Italia Tel. 0039 0382 986335/8/1 Facs 0039 0382 303673

[ccp4bb] Freezing under oil - Thanks and summary

? If its sodium acetate and ammonium sulphate I would try adding sodium malonate as a cryo protectant (with 2-2.3M ammonium sulphate adding 0.6-0.8M Na malonate should be sufficient) or increasing the ammonium sulphate concentration. Claudia Scotti Dipartimento di Medicina Sperimentale

Re: [ccp4bb] Warren DeLano

Claudia Scotti Dipartimento di Medicina Sperimentale Sezione di Patologia Generale Universita' di Pavia Piazza Botta, 10 27100 Pavia Italia Tel. 0039 0382 986335/8/1 Facs 0039 0382 303673 Date: Thu, 5 Nov 2009 10:54:13 -0800 From: brun...@stanford.edu Subject: [ccp4bb] Warren DeLano To: CCP4BB

[ccp4bb] Linux flavour and hard disks

Claudia Scotti Dipartimento di Medicina Sperimentale Sezione di Patologia Generale Universita' di Pavia Piazza Botta, 10 27100 Pavia Italia Tel. 0039 0382 986335/8/1 Facs 0039 0382 303673 _ More than messages–check out the rest

[ccp4bb] Off-topic: modelling of a transmembrane domain

-Rosetta models it as a beta-sheet. Please, would anyone have any suggestions on how to proceed or might please someone indicate a more appropriate forum/list for modelling problems? Many many thanks, Claudia Claudia Scotti Dipartimento di Medicina Sperimentale Sezione di Patologia

[ccp4bb] Contact distances and medium resolution structures

Dear List, Please, is there anybody who could suggest me a good review or some references concerning the relationship between the exerimental uncertainity of distances in medium resolution crystal structures (specifically 2.5-2.9 A) and appropriate cut-off distances for hydrogen bonds,

[ccp4bb] How to replace 2.45M K/Na phosphate buffer - SUMMARY

once the crystals have grown to try and stabilize your crystals at a lower phosphate concentration or in the presence of the cryoprotectant. Claudia Scotti Dipartimento di Medicina Sperimentale Sezione di Patologia Generale Universita' di Pavia Piazza Botta, 10 27100 Pavia Italia Tel. 0039 0382

[ccp4bb] How to replace 2.45M K/Na phosphate buffer

it is acting as a buffer but also as a precipitant and I was wondering if anybody has experienced nice buffer-salt combinations that proved to be useful starting from a similar condition. Thanks a lot, Claudia Claudia Scotti Dipartimento di Medicina Sperimentale Sezione di