-anchored proteins,
particularly, cases where the structural basis for the membrane requirement
is understood.
Sincerely,
Katya Heldwein, Ph.D.
Department of Molecular Biology and Microbiology
Tufts University School of Medicine
136 Harrison Ave
Boston, MA 02111
A related question: how do most people amplify their baculovirus
stocks? Adherent cultures vs suspension? Fold dilution at each stage
(P1 to P2, P2 to P3)? Duration of each amplification stage?
We have some viral stocks that go off rather quickly (1-2 months)
despite being stored with FBS in a
and
biophysical
characterization is highly desirable. Strong communication skills in oral
and written English are essential. To apply, please, send a letter of
interest, a CV, and names of 3 references to Dr. Katya Heldwein by e-mail:
katya.heldw...@tufts.edu
Hi Rongjin,
I had crystals that grew in 9% ethanol as a precipitant. To cryoprotect
them, I first replaced the mother liquor with solution containing 10% MPD,
instead of ethanol, and then briefly dunked the crystals into ~30% MPD.
Hope this helps. Good luck.
Katya
On Mon, Sep 29, 2008 at 1:24
Dear Ngo,
You could try additive screens, such as those sold by Hampton Research.
Using an additive identified during screening, I was able to optimize
conditions giving thin, fiber-like needles to get chunky plates. This was
with a soluble protein, though. I don't know whether this approach will
