Hi,
For loops, there are servers like
SuperLooper http://bioinf-applied.charite.de/superlooper/
ModLoop http://modbase.compbio.ucsf.edu/modloop/
FALC-Loop http://falc-loop.seoklab.org/ (seems to be down)
If you want model flexible region itself, there is a program called
You can check the following site.
http://qa.nmrwiki.org/
http://nmrwiki.org/wiki/index.php?title=Blogs
+--+
Cho, Min-Kyu
Postdoctoral scholar
Dept. of Biochemistry (Sanders lab.)
Vanderbilt University School of Medicine, Nashville, TN
Phone: 615-936-3757
|
Hi all,
I have a homotetrameric coiled-coil domain sample with 45aa per each.
While I store this sample at 4oC, the sample looks clear w/o any
particles. But when I took out the sample to my bench at r.t, I can see
there are precipitates (as stack of needle like particles) at the bottom
of
AM
| To: Min-Kyu Cho
| Cc: CCP4BB@jiscmail.ac.uk
| Subject: Re: [ccp4bb] My protein precipitates at r.t and dissolves at 4
| oC
|
| Which kind of buffer you use? If it is Tris, then temperature change will
| cause pH change.
|
| Actually, this is a good way for crystallization
by detergents where
| cloud point anomalously changes with temperature...
|
| Artem
|
| On Mar 12, 2012 1:11 PM, Min-Kyu Cho min-kyu@live.com wrote:
|
| I am using KPi buffer at pH 5.5, 100mM KCl, 2mM beta-mercaptoethanol,
| 0.02% NaN3.
|
| Yes, I agree I should check CD melting
