Hello,
I got crystals of protein-nucleic acid complex, rod-shape, reproducible,
don’t visibly get damaged upon freezing; however they gave diffraction only
to about 12 A. I tried several crystals. My question is whether such
crystals worth optimization. Clearly a 4A diffracting crystal could
Hello,
I am new to building into an EM map, and I wonder if I could get a
recommendation for some standard routine for programs to use. I tried "find
helixes and strands" tool in phenix, it found some secondary structure
elements, however, it seems that there is more that could be done
Dear All,
I have a question regarding SAXS, I collected SAXS data on the protein of
interest and I can generate the molecular envelope, I also solved x-ray
structures of its domains, and I would like to fit my x-ray structures into
SAXS envelope, or alternatively find some program which could
Dear All,
Thanks to everybody who answered my previous question about heavy atoms! I
got it now.
I have some more questions now.
1) I built a loop in Coot, right now it is a separate molecule. I also know
that it is possible to merge molecules, but if I do so, I’ll get my loop
connected to my
Dear All,
I am new to the crystallography and I am seeking for an advice considering
data processing. I have a dataset with 2.8 A resolution. I used hybrid
substructure search program in phenix, I got the heavy atom sites
(selenium), and then I submitted the site to the autosol. With autosol
Dear All,
I have a question that is a little bit related to the previous discussion
about crystallisation of a minority fraction monomers. I wonder if there is
a review of some sort (or anything in principle) that would discuss role of
dimerization (or more broadly oligomerization) in proteins
