Please stop sending email to my address
ewa.skrzypczak-jan...@utoledo.edu
thank you
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resources.
Best regards - Ewa SJ
From: CCP4 bulletin board [CCP4BB@JISCMAIL.AC.UK] on behalf of
Skrzypczak-Jankun, Ewa [ewa.skrzypczak-jan...@utoledo.edu]
Sent: Wednesday, April 23, 2014 3:33 PM
To: CCP4BB@JISCMAIL.AC.UK
Subject: [ccp4bb] books to go
Hi,
I have
Hi,
I have a collection of books that I would gladly give away to someone who may
cherish them. The list is enclosed.
:) Ewa SJ
Ewa Skrzypczak-Jankun, PhD
DH r.2250
Prof. emerita
Additional band on a gel might not be caused by his-tag. It is often a result
of different conformation/molecular shape and so the molecule travels with
different speed in the gel. We may wish for a homogenous sample (chemically and
structurally) but this is seldom true
See example:
Int J
I will always remember his singing. I still has the booklet with songs that he
collected years ago and wonder if it will ever be published for the
crystallographic community. Those evenings of singing with Lodovico were
wonderful and jolly celebrations of life.
If we want to celebrate his
I have seen proteins refined as 'the same', modeled to an averaged map
etc only to have one of them with much higher Bj because most likely
they are NOT the same so watch out by treating them as 'the same' you
are losing the very valuable information that you might be looking for
Ewa
What is the best and user-friendly program to calculate a surface of the
interface between two proteins? Or two domains?
Is there any common criteria how to define boundary for that interface
(other than van der Waals distance)?
Thanks in advance for good tips - Ewa
Let us not forget about the very fact that X-ray is an ionizing radiation and a
potent creator of radicals! Please see these references - seeing a crystal that
was purple in the middle (0.3mm radius of beam) and perfectly yellow at edges
(crystal was 0.5mm long) was an eye opening experience.
Have you done both experiments at the same temperature? If cryo - have you used
the same cryoprotectant?
same ionic strength? Did mutations cause changes in the surface charge
distribution/hydrophobic/hydrophilic/ pi...pi interactions - if yes how it can
effect quaternary structure of native.
