Re: [ccp4bb] Peptide solubility issues

2014-03-05 Thread Toufic El Arnaout
(containing up to 5 % solvent), from a stock of 50-100 % solvent at 1 mM.. Btw I forgot.. for SPR (depends on the system, and especially for a 12mer), some detergents and even a minor DMSO/ethanol can affect the measurements drastically. Best wishes toufic el arnaout On Wed, Mar 5, 2014 at 12:19 AM

Re: [ccp4bb] delete subject

2013-03-29 Thread Toufic El Arnaout
. Scott -- ** Toufic El Arnaout Trinity Biomedical Science Institute (TCD) 152-160 Pearse Street, Dublin 2 **

Re: [ccp4bb] Oligomerization state

2013-03-21 Thread Toufic El Arnaout
background optimization, also trying different hydrophobic compounds/detergents etc ** Toufic El Arnaout Trinity Biomedical Science Institute (TCD) 152-160 Pearse Street, Dublin 2, Ireland

Re: [ccp4bb] Thrombin cleavage of membrane protein with fusion tag

2013-02-20 Thread Toufic El Arnaout
with their favorite membrane proteins. Many thanks. Raji -- Raji Edayathumangalam Instructor in Neurology, Harvard Medical School Research Associate, Brigham and Women's Hospital Visiting Research Scholar, Brandeis University -- ** Toufic El Arnaout

Re: [ccp4bb] CCP4MG and python.exe

2013-02-16 Thread Toufic El Arnaout
Hi Rex, Maybe it is related to a 3rd party package then.. Are you using Windows? Check C++ (https://www.microsoft.com/en-us/download/details.aspx?id=) or mySQL (https://www.mysql.com/downloads/) Try to reinstall these, probably might help, but be sure about the 64 or 32 bit version. toufic

Re: [ccp4bb] Faculty positions at Molecular Biophysics Unit, IISc, Bangalore

2012-10-30 Thread Toufic El Arnaout
There is a big difference between reading does not discriminate on the basis of race, color, religion, sex, age, sexual orientation, marital status, national origin, disability, or status as a disabled, Vietnam-era, or other eligible veteran and below 35 years. Then regarding Prof. Chitta's reply,

Re: [ccp4bb] inflluence of pH for crystallization on protein 3-D structure

2012-10-28 Thread Toufic El Arnaout
. I can't remember the details but a virus protein crystal structure was once crystallized at both pH 4 and pH 7 which gave totally unrelated structures. Functional studies are important before the interpretation of the structure. Regards Toufic El Arnaout Membrane Structural and Functional Biology

[ccp4bb] Nobel Prize 2012

2012-10-10 Thread Toufic El Arnaout
Congratulations to Robert Lefkowitz and Brian Kobilka: Nobel Prize in Chemistry, 2012! Congrats to the protein structure community. toufic el arnaout

Re: [ccp4bb] [OT]: Purification in ArcticExpress

2012-09-24 Thread Toufic El Arnaout
Toufic El Arnaout Membrane Structural and Functional Biology Group Trinity College Dublin On Mon, Sep 24, 2012 at 3:38 PM, Katherine Sippel katherine.sip...@gmail.com wrote: Hi All, I've recently swapped over to expression in the ArcticExpress(DE3) cells for a particularly rock-like

Re: [ccp4bb] the lysozyme of membrane proteins?

2012-09-11 Thread Toufic El Arnaout
. Regards Toufic El Arnaout Membrane Structural and Functional Biology Group Trinity College Dublin On Tue, Sep 11, 2012 at 10:18 PM, Ho Leung Ng h...@hawaii.edu wrote: Hello, I am developing an undergraduate biochemistry lab class and would like to incorporate experiments with membrane