Hi,
Just for info if you were to use the LCP method (a course by itself), check
this about OmpF ("membrane lysozyme"):
http://www.sciencedirect.com/science/article/pii/S1047847712000834
bR protein sometimes takes weeks to give crystals and people prefer the
dark (depends on the conf state).. but good idea for spectro assays (check
reaction centres/light-harvesting complexes too).
Beta barrels are very stable too.
If you want to use the GFP fusion and you want to cleave it, it might add
extra time and steps to the students than going directly with a GFP free
his-tagged protein to trials.
RegardsToufic El Arnaout Membrane Structural and Functional Biology Group Trinity College Dublin On Tue, Sep 11, 2012 at 10:18 PM, Ho Leung Ng <[email protected]> wrote: > Hello, > > I am developing an undergraduate biochemistry lab class and > would like to incorporate experiments with membrane proteins. Does > anyone have suggestions on membrane proteins that are relatively easy > to express, purify, and assay? Bonus points for crystallizable! At the > moment, my leading candidate is aquaporin AqpZ from E. coli. I am > planning to express the membrane protein as a GFP fusion so students > can easily follow it through the course of the labs. > > > Thank you, > Ho > > Ho Leung Ng > University of Hawaii at Manoa > Assistant Professor, Department of Chemistry > [email protected] >
