my DNA contains a 9-1-9 palindromic sequence,i tried different blunt end
DNA,and got two kind of crystal with different lenght DNA.both don't
diffract.
2011/11/21 Michael Murphy pn1...@gmail.com
Deng, could you tell us a bit more about the DNA that you used? I think
you may want to try to
Hi all,
.
recently,I got a crystal of protein-DNA crystal.i used silver stainto prove
that it is a protein crystal.Does anyone have method to detect if there is
DNA in the crystal.
any suggestion will be appreciated.
Regards,
deng
Hi all,
usually researchers detect the helicase activity using radio-labeled
nucleic acid.
if we increase the necleic acid lenght and concentration in the reaction
mixture,can we employ non radio-labeled nucleic acid?
Best regards,
dengzq.
hello,everybody . due to excess nucleation,I often get many tiny crystals
instead of few,large crystals.i wana optimize the condition, does anyone
have adivce about this?
Best regards.
by using oil (such as parafin oil ... etc ) at reservoir
on top of the reservoir solution. You have to try several trials to find
optimum ratio.
With regards
Syed
--- On *Thu, 5/6/10, zq deng dengzq1...@gmail.com* wrote:
From: zq deng dengzq1...@gmail.com
Subject: [ccp4bb] control
hello everyone， recently i purify a protein conteining zinc binding
domain,and i want to determine its structure.i get the crystal,but poor
diffraction.so i try to adding zinc into the protein to optimize the
crystal,but the protein precipitate immidiately even the znic is 1
mM.BTW,we use the
