The site swift.cmbi.ru.nl/gv/numbers/ is not official, unpublished, and
still poorly maintained. But just for the fun of it, I added a list of
modified cysteines. Be aware that the files are big, so better
download them and read them in the editor than opening them in the browser.
Gert
On
[biophysics.w...@gmail.com]
Sent: Tuesday, May 12, 2015 11:57 PM
To: CCP4BB@JISCMAIL.AC.UK
Subject: Re: [ccp4bb] CYS mod
Hi All,
I have same type of modified CYS !!!
No BME.
On Tue, May 12, 2015 at 2:59 PM, David Schuller schul...@cornell.edu
wrote:
So far almost everyone
:* Tuesday, May 12, 2015 11:57 PM
*To:* CCP4BB@JISCMAIL.AC.UK
*Subject:* Re: [ccp4bb] CYS mod
Hi All,
I have same type of modified CYS !!!
No BME.
On Tue, May 12, 2015 at 2:59 PM, David Schuller schul...@cornell.edu
wrote:
So far almost everyone is suggesting BME.
I didn't do
*To:* CCP4BB@JISCMAIL.AC.UK mailto:CCP4BB@JISCMAIL.AC.UK
*Subject:* Re: [ccp4bb] CYS mod
Hi All,
I have same type of modified CYS !!!
No BME.
On Tue, May 12, 2015 at 2:59 PM, David Schuller
schul...@cornell.edu mailto:schul
.
Best,
Herman
Von: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] Im Auftrag von Bio
Physics
Gesendet: Mittwoch, 13. Mai 2015 00:16
An: CCP4BB@JISCMAIL.AC.UK
Betreff: Re: [ccp4bb] CYS mod
No, it is not sulfenic acid or so, I had that in differend structure. It is L
shaped from S, and linear
Smells like BME to me...
On May 12, 2015 2:32 PM, David Schuller schul...@cornell.edu wrote:
What are the most likely modifications of a CYS residue? I am attaching
images of a couple of residues in my current structure. Blue is 2Fo-Fc,
Green is Fo-Fc positive difference, purple is
BME around ?
Jürgen
..
Jürgen Bosch
Johns Hopkins University
Bloomberg School of Public Health
Department of Biochemistry Molecular Biology
Johns Hopkins Malaria Research Institute
615 North Wolfe Street, W8708
Baltimore, MD 21205
Office: +1-410-614-4742tel:%2B1-410-614-4742
So far almost everyone is suggesting BME.
I didn't do the purification crystallization myself, but I am told:
Buffer was HEPES
cryo was ethylene glycol, glycerol and DMSO.
Some MgSO4 and KPO4
No BME, but maybe some DTT.
Purified from a proteobacterium.
On 05/12/15 15:20, Dyda wrote:
What
11:57 PM
To: CCP4BB@JISCMAIL.AC.UK
Subject: Re: [ccp4bb] CYS mod
Hi All,
I have same type of modified CYS !!!
No BME.
On Tue, May 12, 2015 at 2:59 PM, David Schuller
schul...@cornell.edu wrote:
So far almost everyone is suggesting BME.
I didn't do the purification crystallization
of Bio
Physics [biophysics.w...@gmail.com]
*Sent:* Tuesday, May 12, 2015 11:57 PM
*To:* CCP4BB@JISCMAIL.AC.UK
*Subject:* Re: [ccp4bb] CYS mod
Hi All,
I have same type of modified CYS !!!
No BME.
On Tue, May 12, 2015 at 2:59 PM, David Schuller schul...@cornell.edu
wrote:
So far
...@gmail.com]
*Sent:* Tuesday, May 12, 2015 11:57 PM
*To:* CCP4BB@JISCMAIL.AC.UK
*Subject:* Re: [ccp4bb] CYS mod
Hi All,
I have same type of modified CYS !!!
No BME.
On Tue, May 12, 2015 at 2:59 PM, David Schuller schul...@cornell.edu
wrote:
So far almost everyone is suggesting BME
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