Re: [ccp4bb] CYS mod

2015-05-18 Thread Gert Vriend
The site swift.cmbi.ru.nl/gv/numbers/ is not official, unpublished, and still poorly maintained. But just for the fun of it, I added a list of modified cysteines. Be aware that the files are big, so better download them and read them in the editor than opening them in the browser. Gert On

Re: [ccp4bb] CYS mod

2015-05-13 Thread Bio Physics
[biophysics.w...@gmail.com] Sent: Tuesday, May 12, 2015 11:57 PM To: CCP4BB@JISCMAIL.AC.UK Subject: Re: [ccp4bb] CYS mod Hi All, I have same type of modified CYS !!! No BME. On Tue, May 12, 2015 at 2:59 PM, David Schuller schul...@cornell.edu wrote: So far almost everyone

Re: [ccp4bb] CYS mod

2015-05-13 Thread Bio Physics
:* Tuesday, May 12, 2015 11:57 PM *To:* CCP4BB@JISCMAIL.AC.UK *Subject:* Re: [ccp4bb] CYS mod Hi All, I have same type of modified CYS !!! No BME. On Tue, May 12, 2015 at 2:59 PM, David Schuller schul...@cornell.edu wrote: So far almost everyone is suggesting BME. I didn't do

Re: [ccp4bb] CYS mod

2015-05-13 Thread Roger Rowlett
*To:* CCP4BB@JISCMAIL.AC.UK mailto:CCP4BB@JISCMAIL.AC.UK *Subject:* Re: [ccp4bb] CYS mod Hi All, I have same type of modified CYS !!! No BME. On Tue, May 12, 2015 at 2:59 PM, David Schuller schul...@cornell.edu mailto:schul

[ccp4bb] AW: [ccp4bb] CYS mod

2015-05-13 Thread Herman . Schreuder
. Best, Herman Von: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] Im Auftrag von Bio Physics Gesendet: Mittwoch, 13. Mai 2015 00:16 An: CCP4BB@JISCMAIL.AC.UK Betreff: Re: [ccp4bb] CYS mod No, it is not sulfenic acid or so, I had that in differend structure. It is L shaped from S, and linear

Re: [ccp4bb] CYS mod

2015-05-12 Thread Artem Evdokimov
Smells like BME to me... On May 12, 2015 2:32 PM, David Schuller schul...@cornell.edu wrote: What are the most likely modifications of a CYS residue? I am attaching images of a couple of residues in my current structure. Blue is 2Fo-Fc, Green is Fo-Fc positive difference, purple is

Re: [ccp4bb] CYS mod

2015-05-12 Thread Jurgen Bosch
BME around ? Jürgen .. Jürgen Bosch Johns Hopkins University Bloomberg School of Public Health Department of Biochemistry Molecular Biology Johns Hopkins Malaria Research Institute 615 North Wolfe Street, W8708 Baltimore, MD 21205 Office: +1-410-614-4742tel:%2B1-410-614-4742

Re: [ccp4bb] CYS mod

2015-05-12 Thread David Schuller
So far almost everyone is suggesting BME. I didn't do the purification crystallization myself, but I am told: Buffer was HEPES cryo was ethylene glycol, glycerol and DMSO. Some MgSO4 and KPO4 No BME, but maybe some DTT. Purified from a proteobacterium. On 05/12/15 15:20, Dyda wrote: What

Re: [ccp4bb] CYS mod

2015-05-12 Thread Boaz Shaanan
11:57 PM To: CCP4BB@JISCMAIL.AC.UK Subject: Re: [ccp4bb] CYS mod Hi All, I have same type of modified CYS !!! No BME. On Tue, May 12, 2015 at 2:59 PM, David Schuller schul...@cornell.edu wrote: So far almost everyone is suggesting BME. I didn't do the purification crystallization

Re: [ccp4bb] CYS mod

2015-05-12 Thread Artem Evdokimov
of Bio Physics [biophysics.w...@gmail.com] *Sent:* Tuesday, May 12, 2015 11:57 PM *To:* CCP4BB@JISCMAIL.AC.UK *Subject:* Re: [ccp4bb] CYS mod Hi All, I have same type of modified CYS !!! No BME. On Tue, May 12, 2015 at 2:59 PM, David Schuller schul...@cornell.edu wrote: So far

Re: [ccp4bb] CYS mod

2015-05-12 Thread Bio Physics
...@gmail.com] *Sent:* Tuesday, May 12, 2015 11:57 PM *To:* CCP4BB@JISCMAIL.AC.UK *Subject:* Re: [ccp4bb] CYS mod Hi All, I have same type of modified CYS !!! No BME. On Tue, May 12, 2015 at 2:59 PM, David Schuller schul...@cornell.edu wrote: So far almost everyone is suggesting BME