Hi Lingyuan
I would certainly make a seed stock and use it with RANDOM screens - this
will (usually) allow you to pick up new conditions and control the number
of crystals per drop.
However you say your crystals melt easily when you harvest them. I don't
know how David stabilized his seedstock
I have been presented with crystals grown in nmr tubes on two separate
occasions. Both diffracted very well and thankfully, neither required a
magnetic field for optimisation. On the first occasion I did use the initial
sample as a seed stock.
Best wishes,
David
From: CCP4 bulletin board
Yes, it is many years ago, a large protein batch (50 ml or so) stored in
the cold room that produced an impressive layer of crystals at the
bottom of the flask.
One student at that time tested many crystals and found only a few that
diffracted to atomic (!) resolution.
Crystallization is
Hi Lingyuan,
1) you're very lucky (but you already know that)
2) crystals grown quickly in bulk rarely diffract well. I would follow up
by a conventional droplet crystallization against a concentrating agent -
in the cold - such that your protein gradually concentrates from [X] to 2-3
times [X]
Dear all users,
I got my crystals at the bottom of the tube on ice just before I started to
screen crystals. Those crystals' shapes were good but easy to melt when I
harvested them. I shot these crystals. They only diffracted to 7A to 8A. Has
anyone ever met this situation before? How to
