Hi, everyone,
I can get my protein complex but there are some non-specific aggregation
from the NMR spectra, and chaps can improve it.
So, besides chaps, is there any other detergents to be used during crystal
screening? All suggestions are welcome.
ThanksRegards,
Yuan
download the formulation table and then you will have a nice long
list of possibilities.
Mike
- Original Message -
From: 商元 shangyuan5...@gmail.com
To: CCP4BB@JISCMAIL.AC.UK
Sent: Saturday, August 27, 2011 4:55:27 AM GMT -08:00 US/Canada Pacific
Subject: [ccp4bb] Detergents to eliminate
Hi all,
I have been trying to purify cytosolic fraction of membrane protein whose
domain boundries are unknown.
hence I have made a series of deletion constructs. The expression and
purification is not a problem.
I get good yields of the proteins. But on a gelfiltration column, they run
in the
Hi,
I had set up crystallization with a bicine as buffer and peg 400 as
precipitant. I used the detergent DDAO/LDAO as an additive to the
crystallization drop (one of the hampton additive screen condition, it says
5% on the vial)
I have a clear drop and in the centre there is a shiny precipitate
Rashmi,
Yes, you should use the needle for seeding.
The first thing to do is to seed your control drop to make sure that the
needles
are not bicine, as this buffer can give needles at certain pH at high
PEG400
concentrations.
Next (or at the same time) also seed other protein drops that you
