4BB@JISCMAIL.AC.UK
*Subject: *[ccp4bb] Looking for proteins for undergraduate
biochemistry lab
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Loll
Date: Thursday, June 17, 2021 at 12:27 PM
To: CCP4BB@JISCMAIL.AC.UK
Subject: Re: [ccp4bb] Looking for proteins for undergraduate biochemistry lab
Transglutaminase, also known in some circles as “meat glue."
> On 17 Jun 2021, at 11:50 AM, Bryan Lepore wrote:
>
> Greetings
>
&g
Transglutaminase, also known in some circles as “meat glue."
> On 17 Jun 2021, at 11:50 AM, Bryan Lepore wrote:
>
> Greetings
>
> This enzyme meets none of the stipulations, but I will point out as it is
> somewhat unusual to find in a grocery store :
>
> A food product called Just Egg
Greetings
This enzyme meets none of the stipulations, but I will point out as it is
somewhat unusual to find in a grocery store :
A food product called Just Egg contains “transglutaminase” as an ingredient.
Make of that what you will.
-Bryan W. Lepore
(No affiliation with Just Egg or anything
All the previously suggested proteins are awesome candidates!
Here are a few more in case you want to add some extra 'zing' to your
curriculum:
- luciferase
- magneto reactive protein(s) that can be isolated with strong magnets
- spy-tag and spycatcher (covalently reactive proteins!)
- split GFP
Annex rooms 3,6,9, 203B, 203C
Office: Schlundt Annex 203A
From: CCP4 bulletin board on behalf of P. H
Date: Wednesday, June 16, 2021 at 5:19 PM
To: CCP4BB@JISCMAIL.AC.UK
Subject: [ccp4bb] Looking for proteins for undergraduate biochemistry lab
WARNING: This message has originated from an Ex
jon
Von: CCP4 bulletin board Im Auftrag von Hans-Petter
Hersleth
Gesendet: Donnerstag, 17. Juni 2021 10:12
An: CCP4BB@JISCMAIL.AC.UK
Betreff: Re: [ccp4bb] Looking for proteins for undergraduate biochemistry lab
We can recommend a small flavoprotein. which we have used for different
biochemistry
We can recommend a small flavoprotein. which we have used for different
biochemistry courses. It is easy to express and purify with IEX snd GF (no
tag), and in the 15 years we have used it, students always get crystals.
We have published it in Biochemistry and molecular biology education:
"A
Rowlett
> *Sent:* Thursday, June 17, 2021 10:45 AM
> *To:* CCP4BB@JISCMAIL.AC.UK
> *Subject:* Re: [ccp4bb] Looking for proteins for undergraduate
> biochemistry lab
>
> Human carbonic anhdydrase II is very expressible in *E. coli,* and
> purifiable in one step via affinity chro
9662 7304
+614 57 539 419
tom.p...@csiro.au
From: CCP4 bulletin board on behalf of Roger Rowlett
Sent: Thursday, June 17, 2021 10:45 AM
To: CCP4BB@JISCMAIL.AC.UK
Subject: Re: [ccp4bb] Looking for proteins for undergraduate biochemistry lab
Human carbonic
K
> *Subject:* [ccp4bb] Looking for proteins for undergraduate biochemistry
> lab
>
>
>
> Hello All,
>
>
>
> We are looking for some candidate proteins for an undergraduate level
> advanced biochemistry lab. They should be expressed in bacteria, simple
> enough to p
for proteins for undergraduate biochemistry lab
Hello All,
We are looking for some candidate proteins for an undergraduate level advanced
biochemistry lab. They should be expressed in bacteria, simple enough to purify
and it will be nice to perform some simple characterization experiments(binding
Hello All,
We are looking for some candidate proteins for an undergraduate level
advanced biochemistry lab. They should be expressed in bacteria, simple
enough to purify and it will be nice to perform some simple
characterization experiments(binding assays, enzymatic assays).
Any suggestions?
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