[ccp4bb] N-terminal sequencing facility?

2011-08-08 Thread Dima Klenchin
Hello, Can anyone recommend a facility that does N-terminal protein sequencing very well? It has to be able to work with PVDF-blotted protein bands as a source. Relatively inexpensive would be a plus. Thanks! - Dima

Re: [ccp4bb] N-terminal sequencing

2011-02-09 Thread pankaj gupta
Dear Daniel, You can get it here as well... University of Pittsburgh Department of Biological Sciences Fifth and Ruskin Aves Clapp Hall 301 Pittsburgh PA 15260 email: hem...@pitt.edu ph: 412.624.0106 or Jack Presley Laboratory Manager UC Davis Molecular Structure Facility

Re: [ccp4bb] N-terminal sequencing

2011-02-09 Thread Prince, D Bryan
: [ccp4bb] N-terminal sequencing Hi, Sorry for non-crystallography related questions. I am seeking protein N-terminal sequencing service. However, the facilities I have worked with previously (Michigan state and UCSD) were both closed. Does anyone know any companies or core facilities that can do

[ccp4bb] N-terminal sequencing facility summary

2011-02-09 Thread Junyu Xiao
Dear all, Thanks for your hearty responses. Below is a summary for the information I received. Again, I really appreciate all the help. Best regards, Junyu 1. Yale University, University of Utah, and Wake Forest University are three that have core facilities which perform N-terminal

[ccp4bb] N-terminal sequencing

2011-02-08 Thread Junyu Xiao
Hi, Sorry for non-crystallography related questions. I am seeking protein N-terminal sequencing service. However, the facilities I have worked with previously (Michigan state and UCSD) were both closed. Does anyone know any companies or core facilities that can do this? Thanks, Junyu

Re: [ccp4bb] N-terminal sequencing

2011-02-08 Thread Edward A. Berry
A colleague here has had good results with the facility at Iowa state: http://www.biotech.iastate.edu/service_facilities/protein.html If you just want to identify the protein, mass spec may be cheaper. The same place will do that, or we have had excellent results with

Re: [ccp4bb] N-terminal sequencing

2011-02-08 Thread Daniel Bonsor
We have used Alphalyse (http://www.alphalyse.com/picknpost.html). Dan

Re: [ccp4bb] N-terminal sequencing

2010-04-16 Thread Andreas Förster
Beyond these (posted three weeks ago), I don't know any. http://www.mail-archive.com/ccp4bb@jiscmail.ac.uk/msg15004.html I use Jeff Keen's service, which very reliable and adaptable. Andreas On 16/04/2010 9:23, F.Xavier Gomis-Rüth wrote: Dear CCP4ers, does anybody know of a company that

Re: [ccp4bb] N-terminal sequencing

2010-04-16 Thread Tim Keys
They offer good rates for academic customers and we are pleased with our first results. All the best, Tim -Original Message- From: CCP4 bulletin board on behalf of Andreas Förster Sent: Fri 16.04.2010 11:25 To: CCP4BB@JISCMAIL.AC.UK Subject: Re: [ccp4bb] N-terminal sequencing Beyond