[ccp4bb] OT: VectorNTI alternatives - SUMMARY

2009-02-05 Thread Darren Hart
Thanks for the 38 replies, both on and off the bboard. I have tested some of them and my favorites so far are ApE and Gentle which are free and quite good. But there may be others that are also good and I missed. Darren Summary: *Firstly*, good advice from Warren DeLano: 1. Be wary of relying

Re: [ccp4bb] OT: VectorNTI alternatives

2009-01-30 Thread Jeffrey Wilson
On Jan 28, 2009, at 3:47 AM, Darren Hart wrote: ps anyone using VNTI might consider a backup of their work by exporting files to .gb format. I don't know if a locked up (expired) version permits this and you will have no notice that it is about to expire. My license recently expired. I

Re: [ccp4bb] OT: VectorNTI alternatives

2009-01-30 Thread Darren Hart
Hello, I spoke to Invitrogen (France) today and they said that, if asked, they would provide a 2 month free license so that people could do exactly this and recover their files. The also said they would send me details on a simple procedure to extract the data from a locked version which I will

Re: [ccp4bb] OT: VectorNTI alternatives

2009-01-30 Thread Thomas Schalch
I'm not sure if it was already mentioned, but for those who use Vector NTI's ContigExpress to assemble and analyze sequencing data, the Staden package (http://staden.sourceforge.net/) is a great alternative. Cheers, Thomas

Re: [ccp4bb] OT: VectorNTI alternatives

2009-01-29 Thread Francis E Reyes
Not sure if it's been mentioned, but I personally use EnzymeX(http://mekentosj.com/enzymex/ ) . Also, I find their PDF library organizer Papers (http://mekentosj.com/papers/ ) to be exceptional. Cheers FR On Jan 28, 2009, at 1:47 AM, Darren Hart wrote: Hello, After several years of

[ccp4bb] OT: VectorNTI alternatives

2009-01-28 Thread Darren Hart
Hello, After several years of offering the molecular biology software VectorNTI free to the academic community (their open access program) and building up a huge user base, Invitrogen have suddenly announced that they will no longer renew these free licences and the existing ones will be left to

Re: [ccp4bb] OT: VectorNTI alternatives

2009-01-28 Thread Sebastiano Pasqualato
Hi Darren, much much easier than VectorNTI is ApE (http://www.biology.utah.edu/jorgensen/wayned/ape/ ), which is multi-platform and very easy to use for simple tasks. Please, could you post a summary of the answers? Thanks, ciao Sebastiano On Jan 28, 2009, at 9:47 AM, Darren Hart wrote:

Re: [ccp4bb] OT: VectorNTI alternatives

2009-01-28 Thread James Stroud
I've used serial cloner (http://serialbasics.free.fr/ Serial_Cloner.html) but not ApE, which I hope to try now that I know of it. One thing that serial cloner has going for it is a nice assembly tool that makes construct design much easier. Otherwise it could be a little less clunky in its

Re: [ccp4bb] OT: VectorNTI alternatives

2009-01-28 Thread Mark Brooks
Hi Darren, My favourite program for editing sequences (apart from Vector NTI, which I suppose I'm going to have to delete soon), is BioEdit: http://www.mbio.ncsu.edu/BioEdit/bioedit.html It has an old fashioned cluttered interface, but does do sequence editing, translation into

Re: [ccp4bb] OT: VectorNTI alternatives

2009-01-28 Thread Yong-Fu Li
I like BioEdit too. It is PC based, downloadable, and very easy to use. It allows copy-paste of a word or text file, and does alignment, translation, back translation, etc, and more. Fabulous program. I also use Lasergene which has the long standing DNA Star, Megalign, but you have to buy a

Re: [ccp4bb] OT: VectorNTI alternatives - 4 Mac?

2009-01-28 Thread Mark Collins
Hi Anybody have suggestions for Mac OsX alternatives? Thanks in advance, Mark

Re: [ccp4bb] OT: VectorNTI alternatives - 4 Mac?

2009-01-28 Thread Peter Keller
On Wed, 28 Jan 2009, Jovine Luca wrote: On 28 Jan 2009, at 16:02, Mark Collins wrote: Hi Anybody have suggestions for Mac OsX alternatives? Thanks in advance, Mark Hi Mark, The latest version of DNA Strider (1.4) runs just fine on both Tiger and Leopard. For more info, you can contact the

Re: [ccp4bb] OT: VectorNTI alternatives - 4 Mac?

2009-01-28 Thread Roger Dodd
Hi all, I've recently come across the program PlasmaDNA which seems pretty good for the basics - http://research.med.helsinki.fi/plasmadna/ . It works under Mac OS X and Windows... and probably Wine on Linux too. Cheers, Roger Original Message Subject: Re: [ccp4bb] OT

Re: [ccp4bb] OT: VectorNTI alternatives

2009-01-28 Thread Darren Hart
Hello, Thanks for all the emails (only some of which reached the bb). It is clear that I am not alone in feeling aggrieved by Invitrogen suddenly introducing licence fees, having first persuaded us to put our files and time into their free product over several years. Once the thread goes quiet,

Re: [ccp4bb] OT: VectorNTI alternatives

2009-01-28 Thread Christian Biertuempfel
Hi Darren, I can recommend another free tool: pDRAW32 ( http://www.acaclone.com/ ). It runs natively under Windows but I am using it with the emulator wine on Linux. Cheers, christian Darren Hart wrote: Hello, After several years of offering the molecular biology software VectorNTI free to

Re: [ccp4bb] OT: VectorNTI alternatives - 4 Mac?

2009-01-28 Thread Cynthia Kinsland
There is GENtle which has a whole slew of tools associated with it. There are versions for several platforms. http://gentle.magnusmanske.de/ If you're used to Vector NTI, it is pretty similar (and open source for the ambitious). _ Cynthia Kinsland, Ph. D. Director,

Re: [ccp4bb] OT: VectorNTI alternatives - 4 Mac?

2009-01-28 Thread yann sterckx
Subject: Re: [ccp4bb] OT: VectorNTI alternatives - 4 Mac? From: Mark Collins mcoll...@convex.hhmi.columbia.edu To: CCP4BB@JISCMAIL.AC.UK Date: Wed Jan 28 15:02:17 2009 Hi Anybody have suggestions for Mac OsX alternatives? Thanks in advance, Mark -- Roger Dodd PhD The Institute of Cancer Research

Re: [ccp4bb] OT: VectorNTI alternatives

2009-01-28 Thread Warren DeLano
From: Darren Hart After several years of offering the molecular biology software VectorNTI free to the academic community (their open access program) and building up a huge user base, Invitrogen have suddenly announced that they will no longer renew these free licences and the existing ones

Re: [ccp4bb] OT: VectorNTI alternatives - 4 Mac?

2009-01-28 Thread Michael Giffin
For basic analysis, editing, etc, i really like ApE, A Plasmid Editor. Versions are available for OS X, linux and Windows. http://www.biology.utah.edu/jorgensen/wayned/ape/ i find it does most everything MacVector does, and a few things MacVector does not do. What VectorNTI functionality are