Dear Remie,
I meant application of GF as an ion exchange column. You can use special ion
exchange columns, but our lab often uses preparative GF columns for this task.
We just load the column, keeping sample volume the void volume. Thus, we do
not concentrate a protein before an ion
I meant application of GF as an ion exchange column.
Oh, my goodness! Ion exchange is something else!
It should read buffer-exchange = desalting column.
On Aug 20, 2014, at 11:48 AM, Alexander Aleshin wrote:
Dear Remie,
I meant application of GF as an ion exchange column. You can use special ion
. (212) 650-6070
www.khayatlab.org
Original message
Date: Wed, 20 Aug 2014 18:57:07 +
From: CCP4 bulletin board CCP4BB@JISCMAIL.AC.UK (on behalf of Alexander
Aleshin aales...@sanfordburnham.org)
Subject: Re: [ccp4bb] Removing PEG3350
To: CCP4BB@JISCMAIL.AC.UK
I meant
Hi,
There is a gray area, where you would refrain from talking about low-MW
contaminants. PEG3350 will be a highly elongated polymer, with way higher
hydrodynamic radius than a globular molecule of the same MW. It also will bind
a lot of H2O. Hence, it might not go through concentrators (but
From: CCP4 bulletin board CCP4BB@JISCMAIL.AC.UK (on behalf of Alexander Aleshin
aales...@sanfordburnham.org)
Subject: Re: [ccp4bb] Removing PEG3350
To: CCP4BB@JISCMAIL.AC.UK
I meant application of GF as an ion exchange
column.
Oh, my goodness! Ion exchange is something else
://www.khayatlab.org
Original message
Date: Wed, 20 Aug 2014 18:57:07 +
From: CCP4 bulletin board CCP4BB@JISCMAIL.AC.UKmailto:CCP4BB@JISCMAIL.AC.UK
(on behalf of Alexander Aleshin
aales...@sanfordburnham.orgmailto:aales...@sanfordburnham.org)
Subject: Re: [ccp4bb] Removing PEG3350
To: CCP4BB
@JISCMAIL.AC.UK (on behalf of Alexander
Aleshin aales...@sanfordburnham.org)
Subject: Re: [ccp4bb] Removing PEG3350
To: CCP4BB@JISCMAIL.AC.UK
I meant application of GF as an ion exchange
column.
Oh, my goodness! Ion exchange is something else!
It should read buffer-exchange
...@sanfordburnham.org)
Subject: Re: [ccp4bb] Removing PEG3350
To: CCP4BB@JISCMAIL.AC.UKmailto:CCP4BB@JISCMAIL.AC.UK
I meant application of GF as an ion exchange
column.
Oh, my goodness! Ion exchange is something else!
It should read buffer-exchange = desalting column.
On Aug 20, 2014, at 11:48 AM
)
Subject: Re: [ccp4bb] Removing PEG3350
To: CCP4BB@JISCMAIL.AC.UKmailto:CCP4BB@JISCMAIL.AC.UK
I meant application of GF as an ion exchange
column.
Oh, my goodness! Ion exchange is something else!
It should read buffer-exchange = desalting column.
On Aug 20, 2014, at 11:48 AM
/
Original message
Date: Wed, 20 Aug 2014 18:57:07 +
From: CCP4 bulletin board
CCP4BB@JISCMAIL.AC.UKmailto:CCP4BB@JISCMAIL.AC.UK (on behalf of
Alexander Aleshin
aales...@sanfordburnham.orgmailto:aales...@sanfordburnham.org)
Subject: Re: [ccp4bb] Removing PEG3350
To: CCP4BB
@JISCMAIL.AC.UK
Subject: [ccp4bb] Removing PEG3350
Hi,
Does anyone have a protocol for getting rid of PEG3350 from a protein sample?
Best wishes,
Reza
Reza Khayat, PhD
Assistant Professor
The City College of New York
Department of Chemistry, MR-1135
160 Convent Avenue
New York, NY 10031
Tel. (212
Hi,
Does anyone have a protocol for getting rid of PEG3350 from a protein sample?
Best wishes,
Reza
Reza Khayat, PhD
Assistant Professor
The City College of New York
Department of Chemistry, MR-1135
160 Convent Avenue
New York, NY 10031
Tel. (212) 650-6070
www.khayatlab.org
Remie,
Actually, concentrating of a protein solution is not the best approach to
removing low MW impurities, gel filtration chromatography is more reliable and
... faster.
Regards,
Alex
On Aug 19, 2014, at 7:03 AM, Remie Fawaz-Touma wrote:
Hi Reza, I had to do this before.
This protocol
Reza,
If your protein is not too small (20 kDa), use a spin-column (i.e., desalting
column) with G-25 sephedex. It is CHEAP, fast, and the recovery is good. We
have even used them to adjust buffer concentrations or to remove micellar
detergents; we have used protein concentrations up to 10
Hi Alex,
I disagree with you even though GF is always the last step in my purifications.
Because it involves concentration before and after the GF so during the
concentration you can already be doing the buffer exchange.
You use GF when you want to purify other protein impurities if they are
15 matches
Mail list logo
