Re: [ccp4bb] Staining Crystals with comassie

2013-10-16 Thread Danilo Belviso
Dear All, izit dye is a solution containing methylene blue that you could prepare in your lab. I usually prepare a solution of 0.05%w/v of dye in water and then I add a volume of dye solution equals to 10% of the volume of the drop containing the crystal to test. I prefer to add the dye

Re: [ccp4bb] Staining Crystals with comassie

2013-10-16 Thread Danilo Belviso
Dear Swastik, izit dye is a solution containing methylene blue that you could prepare in your lab. I usually prepare a solution of 0.05%w/v of dye in water and then I add a volume of dye solution equals to 10% of the volume of the drop containing the crystal to test. I prefer to add the dye

Re: [ccp4bb] Staining Crystals with comassie

2013-10-16 Thread Zhijie Li
Sent: Wednesday, October 16, 2013 3:53 AM To: CCP4BB@JISCMAIL.AC.UK Subject: Re: [ccp4bb] Staining Crystals with comassie Dear All, izit dye is a solution containing methylene blue that you could prepare in your lab. I usually prepare a solution of 0.05%w/v of dye in water and then I add a volume

Re: [ccp4bb] Staining Crystals with comassie

2013-10-16 Thread R. M. Garavito
: Danilo Belviso Sent: Wednesday, October 16, 2013 3:53 AM To: CCP4BB@JISCMAIL.AC.UK Subject: Re: [ccp4bb] Staining Crystals with comassie Dear All, izit dye is a solution containing methylene blue that you could prepare in your lab. I usually prepare a solution of 0.05%w/v of dye in water

[ccp4bb] Staining Crystals with comassie

2013-10-15 Thread Swastik Phulera
Dear All, I am looking for a method to quickly differentiate between salt and protein crystals. I have been told thats its a popular alternative to the commercially available izit dye. I would appreciate if some one would share their comassie crystal staining protocol. Swastik

Re: [ccp4bb] Staining Crystals with comassie

2013-10-15 Thread Zhijie Li
reliable. Salt crystals react to attacks from glass fibres like rocks (as indeed what they are). From: Swastik Phulera Sent: Tuesday, October 15, 2013 3:59 AM To: CCP4BB@JISCMAIL.AC.UK Subject: [ccp4bb] Staining Crystals with comassie Dear All, I am looking for a method to quickly differentiate