Hi Everyone,
Sorry for the naive and non-CCP4 question.
Is it possible to precipitate proteins (TCA, acetone) from a sample
that has already been stored in protein loading dye? The protein is
too dilute in my current sample and I basically want to load all of
the sample (100uL) in a single well
Are you sure that 100 uL won't fit in your
loading well? Commercial 10x10 cm minigels have quite large
loading wells. I think it's possible to squeeze as much as 50 uL
into a standard 10-well, 1 mm gel. If you are using typical
discontinuous SDS-PAGE with a stacking
Is it possible to precipitate proteins (TCA, acetone) from a sample
that has already been stored in protein loading dye? The protein is
too dilute in my current sample and I basically want to load all of
the sample (100uL) in a single well in the gel. Unfortunately, I
already added protein dye
Is it ok for you to heat your sample? If so, you could near-boil your
sample in a heating block, say at 95 degrees C, in order to concentrate
it. I've done this in regular 1.5 mL eppendorf tubes with either the cap
open or where I've punctured the top of the cap with a narrow bore needle
(in order
